FastQCFastQC Report
Mon 6 Feb 2017
SRR2077441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298573
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.11744472534032141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51850.026.1797141
GGTATCA21850.025.5747151
TATCAAC68100.019.7696912
ATCAACG69500.019.1806643
TCAACGC69650.019.0144674
CAACGCA70750.018.7798825
CGCACGA1557.221388E-1018.452735
AACGCAG73100.018.1156926
CGAATCG3450.016.58018537
TACCGAT3950.015.59427
ACGCAGA85300.015.4224837
GTATAGT3500.015.0859171
GTACATG89500.015.0437771
CGCAGAG88300.014.749028
CCCTATA3000.014.6657512
TACATGG91600.014.5776932
AGAGTAC85050.014.56383611
GAGTACT43150.014.37881812
GTATATA9400.014.2767881
TATACCG1402.7618995E-514.1415465
Produced by FastQC (version 0.11.5)