FastQCFastQC Report
Mon 6 Feb 2017
SRR2077441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298573
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50950.15446073195894106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33150.10049800322745622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.029.6641791
GTATCAA60500.025.1647031
TCGCGTA1002.4832476E-517.6015479
TATCAAC87300.017.2877222
ATCAACG90650.016.6496073
TCAACGC90500.016.6285824
CAACGCA91250.016.4213185
AACGCAG94400.015.8949836
TCGCACG1552.66773E-715.61474922
CGCGTAA1100.001017978414.0012310
CGTGCCG2855.456968E-1213.89595710
GTACATG83550.013.74564551
TGCGATA2256.715709E-913.6911313
TAGTACT4050.013.5810294
ATACCGT1801.4467205E-613.4452186
ACGCAGA111850.013.2774747
TACATGG84450.013.2079752
GCGTAAC2003.2759272E-713.2011611
GTGGTAT9050.013.1276281
GCGATAC2351.2358214E-813.1085314
Produced by FastQC (version 0.11.5)