##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3298573
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.614985025342776	31.0	31.0	34.0	27.0	34.0
2	30.572729783454847	31.0	31.0	34.0	27.0	34.0
3	30.7373018575002	31.0	31.0	34.0	27.0	34.0
4	34.20479946934629	37.0	35.0	37.0	30.0	37.0
5	33.85215334024743	37.0	35.0	37.0	28.0	37.0
6	33.8054646660844	37.0	35.0	37.0	29.0	37.0
7	33.62351386493493	37.0	35.0	37.0	28.0	37.0
8	33.58864848526924	37.0	35.0	37.0	28.0	37.0
9	35.04239378664653	39.0	35.0	39.0	28.0	39.0
10	34.90452265267435	39.0	35.0	39.0	27.0	39.0
11	34.853658536585364	39.0	35.0	39.0	27.0	39.0
12	34.77529101220437	39.0	35.0	39.0	27.0	39.0
13	34.743722512735054	39.0	35.0	39.0	27.0	39.0
14	35.90022352089828	40.0	36.0	41.0	27.0	41.0
15	35.90665175516807	40.0	36.0	41.0	27.0	41.0
16	35.84778144973599	40.0	36.0	41.0	27.0	41.0
17	35.81683564377687	40.0	36.0	41.0	27.0	41.0
18	35.81132113795875	40.0	36.0	41.0	27.0	41.0
19	35.8492087335948	40.0	36.0	41.0	27.0	41.0
20	35.850045459051536	40.0	36.0	41.0	27.0	41.0
21	35.7907210178462	40.0	36.0	41.0	27.0	41.0
22	35.79059641851188	40.0	36.0	41.0	27.0	41.0
23	35.72351589611629	40.0	36.0	41.0	27.0	41.0
24	35.70721187616584	40.0	36.0	41.0	27.0	41.0
25	35.68830127452083	40.0	36.0	41.0	27.0	41.0
26	35.60037203966685	40.0	35.0	41.0	26.0	41.0
27	35.54489289762573	40.0	35.0	41.0	26.0	41.0
28	35.47869700018766	40.0	35.0	41.0	26.0	41.0
29	35.395816008922644	40.0	35.0	41.0	25.0	41.0
30	35.262520489920945	39.0	35.0	41.0	25.0	41.0
31	35.177581335929204	39.0	35.0	41.0	25.0	41.0
32	35.09337219458232	39.0	35.0	41.0	24.0	41.0
33	34.98903162064323	39.0	35.0	41.0	24.0	41.0
34	34.96144726825812	39.0	35.0	41.0	24.0	41.0
35	34.892849423068704	39.0	35.0	41.0	24.0	41.0
36	34.779705951634234	39.0	34.0	41.0	24.0	41.0
37	34.69076021661488	39.0	34.0	41.0	23.0	41.0
38	34.60847524065709	39.0	34.0	41.0	23.0	41.0
39	34.510663550571714	39.0	34.0	41.0	22.0	41.0
40	34.41220612670995	39.0	34.0	41.0	21.0	41.0
41	34.34495098334946	39.0	34.0	41.0	20.0	41.0
42	34.228784083299054	39.0	34.0	41.0	19.0	41.0
43	34.316435319151644	39.0	34.0	41.0	19.0	41.0
44	34.24386242171994	39.0	34.0	41.0	18.0	41.0
45	34.166741800166314	39.0	34.0	41.0	15.0	41.0
46	34.001509440597495	39.0	34.0	41.0	13.0	41.0
47	33.842485220123976	39.0	33.0	41.0	8.0	41.0
48	33.673802580691714	39.0	33.0	41.0	2.0	41.0
49	33.50942301413369	39.0	33.0	41.0	2.0	41.0
50	33.3561191460671	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6335982520455943
1101	2	0.8136316142969484
1101	3	0.6677174670185053
1101	4	0.7039271502545859
1101	5	0.77158625300126
1101	6	0.8232036859388785
1101	7	0.7515447602465741
1101	8	0.8293871693760622
1101	9	0.845729705703846
1101	10	0.8541308793059059
1101	11	0.9936515500074492
1101	12	0.9252347208607645
1101	13	0.9965302720766118
1101	14	1.1554262516722318
1101	15	1.1477658330018272
1101	16	1.1013352722130207
1101	17	1.120496033546857
1101	18	1.018487648129664
1101	19	1.0561533196007318
1101	20	1.048655614081241
1101	21	1.0726067547135756
1101	22	1.1243271857173909
1101	23	1.1895286040860924
1101	24	1.1426947520234023
1101	25	1.0719926435362908
1101	26	1.0451977580634733
1101	27	1.1551369177493243
1101	28	1.1555925066287926
1101	29	1.1727741715566822
1101	30	1.2333285322520666
1101	31	1.2297357139750105
1101	32	1.262468068074142
1101	33	1.373265638624872
1101	34	1.27098558619236
1101	35	1.1678821115784643
1101	36	1.1396011303702522
1101	37	1.255772865351652
1101	38	1.252681552037707
1101	39	1.2616073845679452
1101	40	1.2071325156621882
1101	41	1.174144707666109
1101	42	1.3858467453642191
1101	43	1.285271269216416
1101	44	1.379174950355889
1101	45	1.293691781854676
1101	46	1.2998630619938254
1101	47	1.3720136309485724
1101	48	1.3637129920079474
1101	49	1.4477157192949335
1101	50	1.3943032873852346
1102	1	0.5254741182582912
1102	2	0.6177123272951484
1102	3	0.5115819172488294
1102	4	0.5450805249791983
1102	5	0.5093520851662916
1102	6	0.5349633494678159
1102	7	0.5442456615083415
1102	8	0.5270319921279167
1102	9	0.648915045179109
1102	10	0.6592967915830883
1102	11	0.69330726801266
1102	12	0.6816923215016644
1102	13	0.7115348785256401
1102	14	0.7131956888843263
1102	15	0.6635212505864487
1102	16	0.7872421017744102
1102	17	0.8620101533145288
1102	18	0.8165587877250928
1102	19	0.7411218355230247
1102	20	0.7338370280608117
1102	21	0.8286307883606767
1102	22	0.7292774761239613
1102	23	0.7748875866616984
1102	24	0.8659543955242981
1102	25	0.7643694911229133
1102	26	0.7667366725437432
1102	27	0.6670161086165436
1102	28	0.7547516093726401
1102	29	0.803231972477974
1102	30	0.7930841901731611
1102	31	0.8328091367669188
1102	32	0.9406138321438391
1102	33	0.8746021497404328
1102	34	0.9829046328576894
1102	35	1.007643978191723
1102	36	0.8853553462725117
1102	37	0.9266872655118732
1102	38	0.7823151190315016
1102	39	0.7725769419482802
1102	40	0.7445036752856566
1102	41	0.7462737282949874
1102	42	0.740642299139509
1102	43	0.8030811230117294
1102	44	0.8753489139048583
1102	45	0.7855009947527378
1102	46	0.8248923830432915
1102	47	0.8633668855049521
1102	48	0.8869000939506719
1102	49	1.0170149782574782
1102	50	0.9924781722240041
1103	1	0.4413246113549363
1103	2	0.6551383627981053
1103	3	0.6262075582744693
1103	4	0.4986876255709163
1103	5	0.5278230910834552
1103	6	0.5880521068642679
1103	7	0.6421857009560696
1103	8	0.6495972781239701
1103	9	0.7270302325558333
1103	10	0.8072127679144501
1103	11	0.7788713705767591
1103	12	0.8163921242630039
1103	13	0.7750812296104499
1103	14	0.8481913496338365
1103	15	0.8612861421051861
1103	16	0.8953935810643472
1103	17	0.9645348081468796
1103	18	0.812356026383874
1103	19	0.816364044596007
1103	20	0.8094007361476017
1103	21	0.9188240822462816
1103	22	0.9222100205026607
1103	23	0.9320816695019332
1103	24	0.8530064665302177
1103	25	0.8279545009848448
1103	26	0.8275725699796439
1103	27	0.8557987516145715
1103	28	0.9754644298854558
1103	29	0.8489436095588374
1103	30	0.9328475037826252
1103	31	0.9725440480095244
1103	32	0.9395917414140555
1103	33	0.8717879485569995
1103	34	0.9391409247709035
1103	35	1.017369816456025
1103	36	1.0132661943987458
1103	37	1.0335424923363306
1103	38	0.9605407600571425
1103	39	0.9514152062480861
1103	40	0.9632299080272801
1103	41	0.9029605133048477
1103	42	0.9031555141296437
1103	43	1.0346878291261277
1103	44	1.081851083530104
1103	45	1.120024466153133
1103	46	1.1743428761399386
1103	47	1.023972013710079
1103	48	1.1406933878362722
1103	49	1.172461132103635
1103	50	1.2841689020070461
1104	1	0.4923777421564317
1104	2	0.650547782730996
1104	3	0.6017343168570548
1104	4	0.566663678750011
1104	5	0.5618441517676658
1104	6	0.5949515962846661
1104	7	0.5877978652301792
1104	8	0.6610396316969656
1104	9	0.7495156328382677
1104	10	0.690227447802485
1104	11	0.7093141240361618
1104	12	0.6278024096505348
1104	13	0.695133311434553
1104	14	0.8550452481399589
1104	15	0.8246381947588262
1104	16	0.8857713867891022
1104	17	0.8833030034614495
1104	18	0.790023626118824
1104	19	0.7747588580499638
1104	20	0.841704217091177
1104	21	0.8900254994282619
1104	22	0.887113323332585
1104	23	0.8157222585520003
1104	24	0.8133649733891417
1104	25	0.8024819299084172
1104	26	0.8040837832195535
1104	27	0.8946736992139961
1104	28	0.8530906887066223
1104	29	0.8923871144955982
1104	30	0.912751183317134
1104	31	0.950572897785527
1104	32	0.9364467410566704
1104	33	0.988137507233084
1104	34	0.891656248920377
1104	35	0.9281072494943672
1104	36	1.0092068643920058
1104	37	1.0174089109575135
1104	38	0.9830802512548118
1104	39	1.0550578430256579
1104	40	1.0186296302317928
1104	41	1.0002606039071296
1104	42	1.0139847085420541
1104	43	0.9675020828550203
1104	44	1.0150059168431582
1104	45	0.8854130417654673
1104	46	0.9606555564027417
1104	47	1.0578628698340395
1104	48	1.1188487717175661
1104	49	1.0802212466218322
1104	50	1.031911668795992
1105	1	0.4439364030783217
1105	2	0.5900127967787157
1105	3	0.457521510363069
1105	4	0.49403608053475523
1105	5	0.5088898003462603
1105	6	0.5858350239592127
1105	7	0.560538619254821
1105	8	0.4958210375113268
1105	9	0.49943742389585566
1105	10	0.5133841155267547
1105	11	0.5250944354617957
1105	12	0.5425852792481436
1105	13	0.5198854262564652
1105	14	0.6206848907622629
1105	15	0.6961246934659471
1105	16	0.6749034476273081
1105	17	0.6233716556619484
1105	18	0.6611024496969264
1105	19	0.634268314396266
1105	20	0.681366918514648
1105	21	0.7868170168426829
1105	22	0.6995960989721652
1105	23	0.7236666163956542
1105	24	0.6935691060094342
1105	25	0.6482899919053864
1105	26	0.586631195235455
1105	27	0.7300060929670877
1105	28	0.8650063824555758
1105	29	0.8699402666876708
1105	30	0.7525442840698702
1105	31	0.7415199348889843
1105	32	0.8787415316743576
1105	33	0.8381724157811234
1105	34	0.7307115186166868
1105	35	0.8266768420414934
1105	36	0.8984251428296375
1105	37	0.8171429775619501
1105	38	0.831682568170784
1105	39	0.8651700561892852
1105	40	0.971715568869385
1105	41	0.9511335092026556
1105	42	0.8955537232396651
1105	43	0.9076964594749555
1105	44	0.950142654123951
1105	45	0.8973269728435014
1105	46	1.0673693783484595
1105	47	1.0028873862874264
1105	48	1.0043435994279761
1105	49	1.0655633350649936
1105	50	1.1597307543836308
1106	1	0.5135939633696189
1106	2	0.5894693456881761
1106	3	0.4785991486137853
1106	4	0.5193856556665182
1106	5	0.4901760929107297
1106	6	0.5795945208134157
1106	7	0.6116961939381582
1106	8	0.579395399678738
1106	9	0.5841169812875293
1106	10	0.5393109251745685
1106	11	0.6005086232885546
1106	12	0.6398948384426149
1106	13	0.6142860885934027
1106	14	0.6640893965319563
1106	15	0.7031429350007699
1106	16	0.8099751124230039
1106	17	0.8839075395681633
1106	18	0.7809874421297422
1106	19	0.6758441975753016
1106	20	0.7433870764635273
1106	21	0.7830664127556446
1106	22	0.7790153270436164
1106	23	0.8579762604274279
1106	24	0.7517749182735756
1106	25	0.8311985327492408
1106	26	0.7296526454382288
1106	27	0.7458188191683348
1106	28	0.8145837487724421
1106	29	0.8488275194286032
1106	30	0.9047395628740276
1106	31	0.8338608308617879
1106	32	0.8299789821922374
1106	33	0.8724054411247479
1106	34	0.7742736551229399
1106	35	0.7764619840000506
1106	36	0.8534339522744503
1106	37	0.8968289886483731
1106	38	0.9600765904739035
1106	39	1.0359351220063644
1106	40	0.9341743432430647
1106	41	0.8801593042872398
1106	42	0.8799741481230541
1106	43	0.8424687319178261
1106	44	0.9595264550471612
1106	45	0.916812320987006
1106	46	0.9497100016299314
1106	47	0.9099972824071756
1106	48	0.9847033853349885
1106	49	0.9652751912294093
1106	50	1.0299387724176157
1107	1	0.30746125863288043
1107	2	0.56860535683753
1107	3	0.41570904603817027
1107	4	0.424409275703276
1107	5	0.48488512243217485
1107	6	0.39037018476420826
1107	7	0.3438774823231725
1107	8	0.3088698372717644
1107	9	0.3225830892030501
1107	10	0.4514689869876065
1107	11	0.3811730722219622
1107	12	0.4579235630540879
1107	13	0.4483076145750715
1107	14	0.46802318453985947
1107	15	0.4273713374758046
1107	16	0.5821603485401923
1107	17	0.47522892141648043
1107	18	0.5247673994261959
1107	19	0.4534092655288191
1107	20	0.44566317210406226
1107	21	0.500056932403929
1107	22	0.444459866546822
1107	23	0.5805542790733611
1107	24	0.5231727388338214
1107	25	0.5493177822988144
1107	26	0.4926970123776897
1107	27	0.5362266898098227
1107	28	0.5381529996024099
1107	29	0.6267988000508637
1107	30	0.6805952927026055
1107	31	0.6213249120131081
1107	32	0.610344397890124
1107	33	0.5722272511113573
1107	34	0.5738767510272211
1107	35	0.6038608154189902
1107	36	0.6121805248784256
1107	37	0.5500765684661104
1107	38	0.5735989189928858
1107	39	0.5472027384340876
1107	40	0.5062023429821281
1107	41	0.48440838749560555
1107	42	0.4787456781702062
1107	43	0.4828042741992178
1107	44	0.4616725671197699
1107	45	0.4243173299525793
1107	46	0.46181568870075296
1107	47	0.49949347364938035
1107	48	0.4848341352713845
1107	49	0.547191923613731
1107	50	0.6329219644618931
1108	1	0.27611109822747437
1108	2	0.4086214182042376
1108	3	0.3011680497603848
1108	4	0.29807220448613236
1108	5	0.29092928085196235
1108	6	0.27381080709493943
1108	7	0.1635356461000228
1108	8	0.15413399520957682
1108	9	0.24236774163519215
1108	10	0.2680391644644473
1108	11	0.26817937895256705
1108	12	0.2520923215016637
1108	13	0.2402729370772576
1108	14	0.25609183680420244
1108	15	0.2975468283984668
1108	16	0.2868485732690189
1108	17	0.1548221718045184
1108	18	0.14590300960490765
1108	19	0.1389640543211783
1108	20	0.21020929308392766
1108	21	0.28559689005559363
1108	22	0.22551414792673796
1108	23	0.2356761845045341
1108	24	0.13439476532398942
1108	25	0.1150523878871681
1108	26	0.13275608702756614
1108	27	0.14032828118973129
1108	28	0.12313342755445689
1108	29	0.21582149482003388
1108	30	0.2771288743025906
1108	31	0.3224072877684634
1108	32	0.14178887066464085
1108	33	0.2727491451179347
1108	34	0.2582861428731036
1108	35	0.2656439781917257
1108	36	0.3237152846392348
1108	37	0.25197108677535596
1108	38	0.22209601271408985
1108	39	0.3062009789282527
1108	40	0.3460358786754938
1108	41	0.24495076989745002
1108	42	0.19433166739836594
1108	43	0.17465616153253194
1108	44	0.1932321188355246
1108	45	0.16850376825042446
1108	46	0.11348221355176946
1108	47	0.16129076840171308
1108	48	0.14244308316484222
1108	49	0.19292160383529477
1108	50	0.296900976538339
1109	1	0.1513987033605666
1109	2	0.3209446700199656
1109	3	0.12385424453558969
1109	4	0.2225889773142029
1109	5	0.19852321600380662
1109	6	0.1691231333350487
1109	7	0.18431621341880344
1109	8	0.16208710598357357
1109	9	0.13616213124626597
1109	10	0.2249140050141989
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2211	5	-0.2503842784700723
2211	6	-0.3397260444715755
2211	7	-0.3320376718249918
2211	8	-0.4118649775690528
2211	9	-0.4391198033057364
2211	10	-0.46234411750781845
2211	11	-0.4941472774418898
2211	12	-0.44203646637712524
2211	13	-0.488139363898604
2211	14	-0.5067452202065823
2211	15	-0.6109726888074931
2211	16	-0.5717897164074088
2211	17	-0.6972322709278913
2211	18	-0.6776139395476335
2211	19	-0.5960160432648536
2211	20	-0.5325584264846412
2211	21	-0.6484298176999275
2211	22	-0.6419967663002808
2211	23	-0.6221018072776943
2211	24	-0.7091562105363067
2211	25	-0.6529592967558742
2211	26	-0.546757266850193
2211	27	-0.5272051035046701
2211	28	-0.4956165724455417
2211	29	-0.6842619669159689
2211	30	-0.7689612643722938
2211	31	-0.7515044995967131
2211	32	-0.668046773916771
2211	33	-0.6184039108656307
2211	34	-0.5484453671423068
2211	35	-0.6398484460506992
2211	36	-0.6195628355456648
2211	37	-0.5352400072153998
2211	38	-0.48351518399880433
2211	39	-0.5583245732032367
2211	40	-0.5820554156234294
2211	41	-0.6731186959474371
2211	42	-0.665841034193825
2211	43	-0.6715431194125117
2211	44	-0.6017601770042305
2211	45	-0.6733747628230233
2211	46	-0.6474833745324631
2211	47	-0.6968467508586826
2211	48	-0.707810512109937
2211	49	-0.8088471429546402
2211	50	-0.8407627368668997
2212	1	-0.7438697041734521
2212	2	-2.1679731961776483
2212	3	-1.1072753602997878
2212	4	-0.5679769489944775
2212	5	-0.423344359540053
2212	6	-0.45372099545459577
2212	7	-0.4925992019762404
2212	8	-0.4106172319930792
2212	9	-0.46089497249914757
2212	10	-0.4058153278433494
2212	11	-0.4701276170786812
2212	12	-0.4568057338902065
2212	13	-1.8517662410972235
2212	14	-1.2311656546631085
2212	15	-0.876811022051541
2212	16	-0.6212186328553315
2212	17	-0.6286714416569836
2212	18	-0.6387545888217545
2212	19	-0.49976965927170625
2212	20	-0.5216254743944972
2212	21	-0.530480584650519
2212	22	-0.49411313475798835
2212	23	-0.5464508144382805
2212	24	-0.5237411063417383
2212	25	-0.47320882355003846
2212	26	-0.4795077980907081
2212	27	-0.5444224299809832
2212	28	-0.5646712545047947
2212	29	-0.5400433368915074
2212	30	-0.5199820050733521
2212	31	-0.4011541710311519
2212	32	-0.45346132008558726
2212	33	-0.4040755952998367
2212	34	-0.4036690461837509
2212	35	-0.5407545682628054
2212	36	-0.5202309039730153
2212	37	-0.5232473642268829
2212	38	-0.5683065270105345
2212	39	-0.3785676268987075
2212	40	-0.2827552915185052
2212	41	-0.41335648187983054
2212	42	-0.42152140150869144
2212	43	-0.53859638631846
2212	44	-0.5506664465351321
2212	45	-0.5529699932653358
2212	46	-0.44889897425390046
2212	47	-0.4934886635031006
2212	48	-0.4351286626786788
2212	49	-0.4444571097409451
2212	50	-0.4591160921641304
2213	1	-0.7370597245792538
2213	2	-2.0203486172472793
2213	3	-1.0277712475561884
2213	4	-0.6870093829181201
2213	5	-0.6010458768168547
2213	6	-0.5679439599580078
2213	7	-0.48757240629490894
2213	8	-0.530718860311822
2213	9	-0.5754808787871823
2213	10	-0.5051185327359349
2213	11	-0.46331648272220605
2213	12	-0.5542960774793286
2213	13	-0.5369224994872965
2213	14	-0.6157674308491607
2213	15	-0.6924267799838049
2213	16	-0.6906706554272333
2213	17	-0.7301865936970273
2213	18	-0.709064022386606
2213	19	-0.7237329165835789
2213	20	-0.8129446297875091
2213	21	-0.7105398955507454
2213	22	-0.7275621868194051
2213	23	-0.82409250348136
2213	24	-0.7508187575229286
2213	25	-0.757987867293707
2213	26	-0.7374118283380895
2213	27	-0.8061308245600216
2213	28	-0.6711898760281088
2213	29	-0.7083380451587118
2213	30	-0.752489002066703
2213	31	-0.6984190819279661
2213	32	-0.6065677865118531
2213	33	-0.7006941857484961
2213	34	-0.7454899977517542
2213	35	-0.7145392473618699
2213	36	-0.7343716378544656
2213	37	-0.6621208081702719
2213	38	-0.6547549182014976
2213	39	-0.7285872751789029
2213	40	-0.6163288154060922
2213	41	-0.5947598205978224
2213	42	-0.7002215456576693
2213	43	-0.7069064529239952
2213	44	-0.67861514645179
2213	45	-0.7122692188471049
2213	46	-0.6874237839173176
2213	47	-0.6519697400094486
2213	48	-0.753279643458697
2213	49	-0.7057628387129284
2213	50	-0.6334342714365846
2214	1	-0.9168258232279598
2214	2	-1.7706678170387704
2214	3	-0.961941156673543
2214	4	-0.6050878385862433
2214	5	-0.5673725491227657
2214	6	-0.5070932138245539
2214	7	-0.48227050779144776
2214	8	-0.49851187368054894
2214	9	-0.5966850328392255
2214	10	-0.6960889310617304
2214	11	-0.589637729061657
2214	12	-0.64609375222566
2214	13	-0.5658401946165483
2214	14	-0.7261663551960353
2214	15	-0.7750481272173388
2214	16	-0.7880298311298262
2214	17	-0.7053430746940492
2214	18	-0.7613149786099953
2214	19	-0.7347323092009859
2214	20	-0.8759857142836367
2214	21	-0.8504587376779398
2214	22	-0.8069415993587725
2214	23	-0.692621404951332
2214	24	-0.7442076095468906
2214	25	-0.7805391103984434
2214	26	-0.7974818178014829
2214	27	-0.7456110027546288
2214	28	-0.791254749121606
2214	29	-0.7712552910289503
2214	30	-0.64801976925731
2214	31	-0.7750235919243238
2214	32	-0.8002346562509359
2214	33	-0.7567055935792482
2214	34	-0.7555194357984405
2214	35	-0.7604677830721727
2214	36	-0.846792342434334
2214	37	-0.6718749346051496
2214	38	-0.9241556435977145
2214	39	-0.885969771529389
2214	40	-0.7623100169320125
2214	41	-0.791960677790442
2214	42	-0.7785763082332267
2214	43	-0.8763492261203183
2214	44	-0.8621091317358633
2214	45	-0.970116832436652
2214	46	-0.8365674570191217
2214	47	-0.8155457729697915
2214	48	-0.7555057574244017
2214	49	-0.7415761666615737
2214	50	-0.7927687167949102
2215	1	-0.3775292811751356
2215	2	-0.16125367837057425
2215	3	-0.45192856433757456
2215	4	-0.37953175075262635
2215	5	-0.3951638732850711
2215	6	-0.47750879406381586
2215	7	-0.549807973996856
2215	8	-0.5783105952186389
2215	9	-0.7372768339521443
2215	10	-0.6414437183319208
2215	11	-0.6580647810902676
2215	12	-0.6362929476201487
2215	13	-0.6515756030607633
2215	14	-0.7961493536085911
2215	15	-0.8036254916231869
2215	16	-0.8149924591321067
2215	17	-0.8229190251557199
2215	18	-0.7783983416422302
2215	19	-0.7320711767527044
2215	20	-0.7337554176426835
2215	21	-0.8155278518659514
2215	22	-0.7966511357740558
2215	23	-0.7152315823656892
2215	24	-0.821420675297226
2215	25	-0.8017955702557487
2215	26	-0.8503165852466239
2215	27	-0.8667009829793031
2215	28	-1.0291843679644757
2215	29	-0.9951395100527165
2215	30	-0.8034974906578114
2215	31	-0.9630103155465832
2215	32	-0.7715234671951592
2215	33	-0.8984966226863875
2215	34	-1.0152477750743216
2215	35	-0.9989216497591684
2215	36	-0.9900935677973592
2215	37	-0.8817761905787762
2215	38	-0.9916274683150519
2215	39	-1.0137998285899599
2215	40	-0.9189850876982888
2215	41	-1.015041285869323
2215	42	-1.0227520351381116
2215	43	-0.9355872433716499
2215	44	-0.8907377716475509
2215	45	-0.9207447087411254
2215	46	-0.8474383062862643
2215	47	-0.8599460512278156
2215	48	-0.8033847030276107
2215	49	-0.7376645307132463
2215	50	-0.9152589382575798
2216	1	-1.385240095683514
2216	2	-1.0314346268601788
2216	3	-1.0717366508901733
2216	4	-2.628428623021641
2216	5	-1.8322026981705264
2216	6	-1.5229022565610677
2216	7	-1.4559068191280389
2216	8	-1.444496875362141
2216	9	-1.5692996502364238
2216	10	-1.503092281056638
2216	11	-1.703952309127807
2216	12	-1.5542715051677973
2216	13	-1.6677831034714288
2216	14	-1.737662422901316
2216	15	-1.862935772649898
2216	16	-1.8525023403440741
2216	17	-1.8892605186553268
2216	18	-2.0032483715799074
2216	19	-1.883348375906742
2216	20	-1.9965588689456766
2216	21	-1.9624707397950658
2216	22	-1.9504451950085482
2216	23	-1.9497907888878103
2216	24	-1.927236016868413
2216	25	-2.072269905988243
2216	26	-2.207292676420039
2216	27	-2.374470012379902
2216	28	-2.2597856734408452
2216	29	-2.2474664156317203
2216	30	-2.271504463793768
2216	31	-2.113106375060589
2216	32	-2.155350203862021
2216	33	-2.160854161752134
2216	34	-2.2037167194554783
2216	35	-2.1844554565999843
2216	36	-2.327385118454302
2216	37	-2.27503105142344
2216	38	-2.4402294905561064
2216	39	-2.588511398014038
2216	40	-2.4562800383421397
2216	41	-2.3777660037544166
2216	42	-2.393279367178266
2216	43	-2.265302674350629
2216	44	-2.183637353627063
2216	45	-2.114901475416829
2216	46	-2.139912850366798
2216	47	-2.1833413414157086
2216	48	-2.2058934584880987
2216	49	-2.2712112725275304
2216	50	-2.300659947935088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74573.0
3	12046.0
4	20821.0
5	12623.0
6	9472.0
7	7574.0
8	6565.0
9	5722.0
10	5580.0
11	5534.0
12	5800.0
13	6072.0
14	6745.0
15	7187.0
16	7716.0
17	8352.0
18	9170.0
19	10062.0
20	12073.0
21	14986.0
22	18777.0
23	22755.0
24	26631.0
25	30105.0
26	32823.0
27	37114.0
28	44050.0
29	50565.0
30	58040.0
31	69627.0
32	84573.0
33	105337.0
34	132337.0
35	169720.0
36	218890.0
37	294286.0
38	467408.0
39	1186862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.295276743682585	18.224035249321886	14.478430954008148	26.00225705298738
2	14.970550750085795	20.1045548415731	37.81315217931726	27.11174222902385
3	17.601356226527663	24.558232006412485	29.601517508831115	28.23889425822873
4	12.508530390016556	16.815001723510164	36.2617443499335	34.41472353653978
5	14.00619222087468	37.36873980814366	34.04835924949725	14.57670872148441
6	32.641365365394506	36.66205132310559	16.998443761947733	13.698139549552172
7	28.388363976447017	31.784817201183774	21.455324757163343	18.371494065205862
8	27.054679251164558	34.03033039461898	19.530331304312494	19.384659049903966
9	26.85131105155232	15.124961259492562	18.71171058066183	39.31201710829329
10	14.677401159412554	28.659373131178107	33.3030581659253	23.360167543484035
11	35.118301516849414	22.61426411649549	23.305800663877513	18.96163370277758
12	23.824889977730177	24.42047052622353	29.919961471355087	21.834678024691208
13	28.37772470525797	21.465195799689145	25.793547897949125	24.36353159710376
14	22.638413612030327	20.526956045445818	26.853018666438594	29.98161167608526
15	23.870588552838804	28.34993095989851	23.699724537121984	24.0797559501407
16	23.862411829770533	27.492673268888225	25.002964660938325	23.641950240402913
17	22.708349888175203	27.27111705299349	26.45495071155996	23.565582347271345
18	22.802562182422804	26.653994667326653	27.524747250027527	23.01869590022302
19	23.690927217740164	26.329045660395558	27.578374210752838	22.40165291111144
20	23.906078162447887	25.738698643224524	27.47417261687759	22.881050577449997
21	23.967612416615257	25.68019566487295	27.484769792294117	22.867422126217672
22	23.745515392111457	25.348039541283264	27.47705491261896	23.429390153986322
23	23.568101231884693	25.405297859389176	27.449522207559696	23.577078701166435
24	23.271297083718	25.620311948397617	27.437544204012358	23.67084676387202
25	23.48751275292028	25.44024111966091	27.556407030756226	23.515839096662585
26	23.339261635252935	25.805249216147867	27.5607504356756	23.294738712923596
27	23.41443293966359	25.829860668813957	27.353162399383717	23.402543992138735
28	23.48445030530422	25.6874083882612	27.40565260919634	23.422488697238236
29	23.585841537825583	25.837758952154182	27.22836011147373	23.3480393985465
30	23.555610818286034	25.80858135853314	27.323844992084624	23.311962831096203
31	23.591726023625114	25.48032205755828	27.31581601753855	23.61213590127806
32	23.391282434317123	25.756766616659828	27.219368082172547	23.6325828668505
33	23.51497262735542	25.723129657246098	27.179304618993527	23.582593096404956
34	23.719976991621106	25.62402040795905	27.301042563378413	23.35496003704143
35	23.673160472444383	25.636493635973306	27.211338568160702	23.47900732342161
36	23.68413471932766	25.75521195785495	27.03198357474827	23.52866974806912
37	23.659659806561905	25.479456374548743	27.280423383098757	23.580460435790595
38	23.691835608463364	25.647305320226323	27.138066331512018	23.5227927397983
39	23.7501428828282	25.918293155844307	26.891563985583467	23.43999997574403
40	23.717994583955363	25.605939700722907	27.200889657489142	23.475176057832588
41	23.54385209619326	25.623673583235306	27.126117221892095	23.70635709867934
42	23.82548544984453	25.74475864544577	26.96784243435573	23.461913470353966
43	23.66722995198432	25.534529875040395	27.05179253880393	23.74644763417135
44	23.738708763015524	25.774443566931893	26.714668792908213	23.772178877144366
45	23.7259607003779	25.81521022402573	26.62866965488911	23.830159420707258
46	23.811793210454802	25.671584597073494	26.539937948342025	23.97668424412968
47	23.693666791468413	25.888894110014505	26.550181669907975	23.867257428609108
48	23.69852299185099	26.13452536864571	26.38754486806364	23.77940677143966
49	23.86391678818397	25.858659964772613	26.373580066513668	23.903843180529744
50	23.93700777853164	25.94682052746557	26.33374624003279	23.78242545397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	829.0
1	605.5
2	382.0
3	608.5
4	835.0
5	948.5
6	1062.0
7	1004.0
8	946.0
9	1260.5
10	1575.0
11	2334.0
12	3093.0
13	6028.0
14	8963.0
15	12207.0
16	15451.0
17	17476.0
18	19501.0
19	20233.5
20	20966.0
21	22201.5
22	23437.0
23	26659.5
24	29882.0
25	34981.5
26	40081.0
27	45816.0
28	51551.0
29	58377.0
30	65203.0
31	72364.0
32	79525.0
33	89238.5
34	98952.0
35	109284.5
36	119617.0
37	128822.5
38	138028.0
39	147802.0
40	157576.0
41	168849.0
42	180122.0
43	188110.5
44	196099.0
45	206114.5
46	216130.0
47	223030.0
48	229930.0
49	232296.0
50	234662.0
51	236973.0
52	239284.0
53	235012.0
54	230740.0
55	221772.5
56	212805.0
57	200010.5
58	187216.0
59	170458.0
60	153700.0
61	134373.0
62	115046.0
63	98474.5
64	81903.0
65	68755.0
66	55607.0
67	45406.0
68	35205.0
69	28556.5
70	21908.0
71	17379.5
72	12851.0
73	10610.5
74	8370.0
75	6344.0
76	4318.0
77	3357.5
78	2397.0
79	1844.0
80	1291.0
81	996.5
82	702.0
83	561.0
84	420.0
85	323.5
86	227.0
87	159.5
88	92.0
89	69.5
90	47.0
91	35.5
92	24.0
93	18.0
94	12.0
95	10.0
96	8.0
97	4.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014157637257080562
2	5.153743755254166E-4
3	2.728452576311029E-4
4	0.002182762061048823
5	0.036682529081514946
6	0.02658725454916414
7	0.02989171378047416
8	0.023009950060223012
9	0.030043294479158107
10	0.03155910146599757
11	0.038744026583616614
12	0.038986555701510925
13	0.03631873540467347
14	0.0415937437188748
15	0.0368037936404621
16	0.0424425956315049
17	0.04398871875808115
18	0.04668685519465538
19	0.04856645585833632
20	0.04520136434755271
21	0.04568642258334134
22	0.04432219629518583
23	0.04335207982360857
24	0.04126026618177012
25	0.03907750412072129
26	0.04229101493282095
27	0.04223038265334737
28	0.04153311143940122
29	0.039229084819405236
30	0.03616715470598953
31	0.034863560697307595
32	0.03104372709047215
33	0.022888685501275853
34	0.01952359399049225
35	0.02003896836601767
36	0.02194888516943539
37	0.017189251230759484
38	0.017977470863916004
39	0.012611514130504313
40	0.006821131440777572
41	0.0064573377639361015
42	0.005729750410253161
43	0.002031181362364877
44	0.003274143091573235
45	0.0020614975021016665
46	9.398003318404656E-4
47	2.4252911789431368E-4
48	3.941098165782598E-4
49	9.094841921036764E-5
50	2.122129781575245E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3298573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.90330287872364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.73124591812757	45.19723818577295
2	13.014516722627809	15.331960406663192
3	4.379813891283777	7.739565125721885
4	1.9727362159804733	4.648027153189
5	1.0688346894139338	3.1478946718917733
6	0.640298108149512	2.262940403820267
7	0.4501758437712034	1.8561790852038118
8	0.3173882543193255	1.4956173179456478
9	0.21686245165453544	1.14965232055467
>10	1.1187933340786143	11.645583218204198
>50	0.06346219313181964	2.5537414956235094
>100	0.025043956423639886	2.361854910909773
>500	6.730920935767123E-4	0.2626789256668267
>1k	1.0355262942865762E-4	0.1916796753042859
>5k	5.177631471432881E-5	0.1553871035282954
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5095	0.15446073195894106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3315	0.10049800322745622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.0316139736789213E-5	0.0
9	0.0	0.0	0.0	3.0316139736789213E-5	0.0
10	3.0316139736789213E-5	0.0	0.0	3.0316139736789213E-5	0.0
11	3.0316139736789213E-5	0.0	0.0	3.0316139736789213E-5	0.0
12	3.0316139736789213E-5	0.0	0.0	3.0316139736789213E-5	0.0
13	3.0316139736789213E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
14	3.0316139736789213E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
15	6.0632279473578426E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
16	6.0632279473578426E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
17	6.0632279473578426E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
18	6.0632279473578426E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
19	6.0632279473578426E-5	3.0316139736789213E-5	0.0	3.0316139736789213E-5	0.0
20	6.0632279473578426E-5	3.0316139736789213E-5	0.0	9.094841921036763E-5	0.0
21	6.0632279473578426E-5	3.0316139736789213E-5	0.0	1.2126455894715685E-4	0.0
22	6.0632279473578426E-5	3.0316139736789213E-5	0.0	1.2126455894715685E-4	0.0
23	6.0632279473578426E-5	3.0316139736789213E-5	0.0	1.2126455894715685E-4	0.0
24	6.0632279473578426E-5	3.0316139736789213E-5	0.0	1.8189683842073526E-4	0.0
25	9.094841921036763E-5	3.0316139736789213E-5	0.0	2.425291178943137E-4	0.0
26	9.094841921036763E-5	3.0316139736789213E-5	0.0	3.031613973678921E-4	0.0
27	9.094841921036763E-5	3.0316139736789213E-5	0.0	3.637936768414705E-4	0.0
28	9.094841921036763E-5	3.0316139736789213E-5	0.0	5.456905152622058E-4	0.0
29	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.0010307487510508332	0.0
30	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.0017583361047337743	0.0
31	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.0033347753710468134	0.0
32	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.005184059894990955	0.0
33	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.007336505816302989	0.0
34	9.094841921036763E-5	3.0316139736789213E-5	0.0	0.010519700488665856	0.0
35	1.5158069868394605E-4	3.0316139736789213E-5	0.0	0.013854475859712669	0.0
36	1.5158069868394605E-4	3.0316139736789213E-5	0.0	0.017674309466548112	0.0
37	1.5158069868394605E-4	6.0632279473578426E-5	0.0	0.02270678866285512	0.0
38	1.5158069868394605E-4	6.0632279473578426E-5	0.0	0.029376339404948746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2670	0.0	29.664179	1
GTATCAA	6050	0.0	25.164703	1
TCGCGTA	100	2.4832476E-5	17.601547	9
TATCAAC	8730	0.0	17.287722	2
ATCAACG	9065	0.0	16.649607	3
TCAACGC	9050	0.0	16.628582	4
CAACGCA	9125	0.0	16.421318	5
AACGCAG	9440	0.0	15.894983	6
TCGCACG	155	2.66773E-7	15.614749	22
CGCGTAA	110	0.0010179784	14.00123	10
CGTGCCG	285	5.456968E-12	13.895957	10
GTACATG	8355	0.0	13.7456455	1
TGCGATA	225	6.715709E-9	13.69113	13
TAGTACT	405	0.0	13.581029	4
ATACCGT	180	1.4467205E-6	13.445218	6
ACGCAGA	11185	0.0	13.277474	7
TACATGG	8445	0.0	13.207975	2
GCGTAAC	200	3.2759272E-7	13.20116	11
GTGGTAT	905	0.0	13.127628	1
GCGATAC	235	1.2358214E-8	13.10853	14
>>END_MODULE