FastQCFastQC Report
Mon 6 Feb 2017
SRR2077440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776198
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.1131296611035756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55950.024.3413121
GGTATCA24700.024.2284341
TCAACGC75850.017.664364
ATCAACG76050.017.5885093
TATCAAC75500.017.5131262
CAACGCA76200.017.436555
AACGCAG78650.016.7806246
TCGATCG1602.14186E-816.4946141
CGTACAC2650.015.7736563
GTACATG97650.015.1633881
TACATGG98850.014.7561482
ACGCAGA90550.014.4548257
TGGACCG3350.014.4462955
CGCAGAG93300.014.0291438
ACATGGG102500.013.9727463
AGAGTAC88950.013.82672411
GAGTACT48150.013.25119812
GTATAAA9800.013.0213291
CAGAGTA97300.012.95689710
TATACCG1902.6598027E-612.7355495
Produced by FastQC (version 0.11.5)