FastQCFastQC Report
Mon 6 Feb 2017
SRR2077440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776198
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52160.13812835026129458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.029.1416871
GTATCAA69700.024.4666421
TATCAAC97300.017.548152
ATCAACG99400.017.2659573
TCAACGC100200.017.1502914
CAACGCA101900.016.82215
AACGCAG105200.016.417526
TGCGATA2001.2769306E-915.4021213
GCGTAAT900.00435576714.6694611
CGAATCG2102.5556801E-914.66382837
CGTGCCG2601.6370905E-1114.38545210
GTACATG94550.014.3591611
GCACCGT4700.014.0435556
AATACCG1100.001017032514.0030395
ACGCAGA122750.013.890467
TACATGG94600.013.8856322
CGCAGAG125150.013.5537648
CGCGATC1952.4080146E-713.53942723
ACCCGTT1302.3319402E-413.538897
ACATGGG98000.013.1793643
Produced by FastQC (version 0.11.5)