##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077439_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 315901 Sequences flagged as poor quality 0 Sequence length 50 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.485607832833704 31.0 22.0 33.0 7.0 34.0 2 25.90922789101649 31.0 23.0 34.0 7.0 34.0 3 26.638693767984275 31.0 25.0 34.0 7.0 34.0 4 28.7856512008509 35.0 25.0 37.0 7.0 37.0 5 30.352154630722918 35.0 31.0 37.0 7.0 37.0 6 29.477212797680284 35.0 28.0 37.0 7.0 37.0 7 29.87735398115232 35.0 30.0 37.0 7.0 37.0 8 29.349657012798314 35.0 27.0 37.0 7.0 37.0 9 30.077631916328215 37.0 27.0 39.0 7.0 39.0 10 30.678627164839618 37.0 29.0 39.0 7.0 39.0 11 30.310173123858423 37.0 27.0 39.0 2.0 39.0 12 30.80182082361246 37.0 29.0 39.0 2.0 39.0 13 30.55144174915559 37.0 28.0 39.0 2.0 39.0 14 31.18021152196416 37.0 28.0 40.0 2.0 41.0 15 31.38607981614493 38.0 30.0 40.0 2.0 41.0 16 31.24559276482189 37.0 30.0 40.0 2.0 41.0 17 31.216533027752366 37.0 30.0 40.0 2.0 41.0 18 30.961855138160374 37.0 30.0 40.0 2.0 41.0 19 31.084716414319676 37.0 30.0 40.0 2.0 41.0 20 30.991718924599795 37.0 28.0 40.0 2.0 41.0 21 30.952288849987813 37.0 28.0 40.0 2.0 41.0 22 30.945267662970362 36.0 28.0 40.0 2.0 41.0 23 30.41976758541442 36.0 27.0 40.0 2.0 41.0 24 30.520555490485943 36.0 27.0 40.0 2.0 41.0 25 30.36645024865385 36.0 27.0 40.0 2.0 41.0 26 30.278951317026536 36.0 25.0 40.0 2.0 41.0 27 30.023130664353705 35.0 25.0 40.0 2.0 41.0 28 30.03662223291474 35.0 25.0 40.0 2.0 41.0 29 29.87666389153564 35.0 25.0 40.0 2.0 41.0 30 29.675132399074393 35.0 25.0 40.0 2.0 41.0 31 29.555221414303848 35.0 25.0 40.0 2.0 41.0 32 29.362876344171116 35.0 25.0 40.0 2.0 41.0 33 29.338634572223576 35.0 25.0 40.0 2.0 41.0 34 29.15062630381037 35.0 25.0 39.0 2.0 41.0 35 29.080560048876073 35.0 25.0 39.0 2.0 41.0 36 28.610874926005298 35.0 25.0 39.0 2.0 41.0 37 28.592555895676178 35.0 25.0 39.0 2.0 41.0 38 28.411663780741435 35.0 25.0 39.0 2.0 41.0 39 28.171522090781604 35.0 25.0 38.0 2.0 40.0 40 28.196938281296987 35.0 25.0 39.0 2.0 40.0 41 28.019746692792996 35.0 25.0 38.0 2.0 40.0 42 27.76107704628982 34.0 24.0 38.0 2.0 40.0 43 27.802656528469363 34.0 24.0 38.0 2.0 40.0 44 27.7163953263839 34.0 24.0 38.0 2.0 40.0 45 27.538143279065277 34.0 23.0 38.0 2.0 40.0 46 27.18871102022469 33.0 22.0 38.0 2.0 40.0 47 26.958657933972987 33.0 22.0 38.0 2.0 40.0 48 26.41457608554579 33.0 20.0 38.0 2.0 40.0 49 26.19659956758605 33.0 20.0 38.0 2.0 40.0 50 25.921712815090803 33.0 15.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1540961673564958 1101 2 1.1414481899321558 1101 3 1.138651820919062 1101 4 1.5126605720270305 1101 5 0.8438025950043233 1101 6 1.4096027887632196 1101 7 1.5891954792864453 1101 8 1.1732133900627346 1101 9 1.8498287054935076 1101 10 1.5906288761099177 1101 11 1.9970222279254735 1101 12 1.97515804445802 1101 13 2.090677428696509 1101 14 1.7419859554405939 1101 15 1.8315118762317901 1101 16 1.706164243385146 1101 17 1.8989264275372868 1101 18 2.015449049642534 1101 19 1.4652992599627055 1101 20 1.9605123354647667 1101 21 2.071709901600876 1101 22 1.8494219332957726 1101 23 1.7852923301423154 1101 24 1.8997259315289625 1101 25 1.9568338326905277 1101 26 1.3558488338237424 1101 27 1.7304242097252427 1101 28 1.983080182717618 1101 29 1.7461091670180906 1101 30 1.8504761237518537 1101 31 1.7587432229431315 1101 32 1.5555723702485622 1101 33 1.6066770585784553 1101 34 1.7216169423251486 1101 35 1.6333943757687912 1101 36 1.6997210256786737 1101 37 1.341983154817047 1101 38 1.5513486742611207 1101 39 1.6415850211528067 1101 40 1.6278802951483797 1101 41 1.7503696535827729 1101 42 1.889714852421843 1101 43 1.9356847837124498 1101 44 1.599067871754162 1101 45 1.9298868733398251 1101 46 2.093227340070424 1101 47 2.040581165879356 1101 48 1.820244222006579 1101 49 2.30173935755467 1101 50 2.1908957720529862 1102 1 1.0968179804329488 1102 2 0.8967418255781361 1102 3 0.8881446911238129 1102 4 1.02528635520531 1102 5 1.0991411978242631 1102 6 1.1742346075355172 1102 7 1.2335850748816597 1102 8 1.5949805000724275 1102 9 1.5318908001643976 1102 10 1.629475518061291 1102 11 1.2004637156394438 1102 12 1.6109039499470157 1102 13 1.17750404526641 1102 14 1.4780886005096967 1102 15 1.8174521398393573 1102 16 1.6769405766618561 1102 17 1.99647022056838 1102 18 1.944930528163212 1102 19 1.489161840279074 1102 20 1.6974743531918257 1102 21 2.024999000558637 1102 22 1.9766440611424514 1102 23 2.0540461398612173 1102 24 2.0528691369889813 1102 25 2.12269775202941 1102 26 1.6457322189525954 1102 27 1.7874825571316357 1102 28 1.6321413680523236 1102 29 1.3930419061185475 1102 30 1.789625544674582 1102 31 1.9390915686507633 1102 32 1.7164299442195627 1102 33 1.5160032504583825 1102 34 1.5905276375176562 1102 35 1.7290195693774564 1102 36 1.6911422881570637 1102 37 1.565824750452233 1102 38 1.7396262316442304 1102 39 1.3779574692656844 1102 40 1.5994310630877848 1102 41 1.585120285621386 1102 42 1.8047868498637136 1102 43 1.8561128712085768 1102 44 1.6675378886418315 1102 45 1.9403742175785297 1102 46 2.0007548449918318 1102 47 2.274690266236096 1102 48 2.401394981951821 1102 49 2.1398025714085556 1102 50 2.364229238622393 1103 1 0.582370542652729 1103 2 1.0974449893287321 1103 3 0.8332337293553032 1103 4 1.1658996540450417 1103 5 0.2503505811214488 1103 6 1.3309535657249896 1103 7 0.9883433441068199 1103 8 1.232608027523046 1103 9 1.1487589496669308 1103 10 1.1226578446288222 1103 11 1.5005872312636832 1103 12 1.1685040510936702 1103 13 1.1759673941931865 1103 14 1.4780788748699898 1103 15 1.0348979923403085 1103 16 0.8548954691449069 1103 17 1.4078988197844886 1103 18 0.5728178274503257 1103 19 0.9208246328992615 1103 20 1.014637189577389 1103 21 0.9340788577862469 1103 22 1.278970434159632 1103 23 1.098482587668574 1103 24 1.2311157986631507 1103 25 0.8339775489580532 1103 26 1.1493793338415799 1103 27 1.0296704370394387 1103 28 1.2681836162311981 1103 29 1.0547890200349634 1103 30 1.2763500464786866 1103 31 1.4063404769477081 1103 32 1.269123460135571 1103 33 1.4679196877620448 1103 34 1.1905461162330937 1103 35 1.23956216281654 1103 36 1.4134606861496906 1103 37 1.4794562070612933 1103 38 1.1450249342438923 1103 39 1.2613859517741233 1103 40 1.4098977965371766 1103 41 0.957262163253386 1103 42 1.3451433918152595 1103 43 1.6818585846328773 1103 44 1.3844439681392124 1103 45 1.1561715741496599 1103 46 1.3110416541067025 1103 47 0.9960020605193627 1103 48 1.5586050849463469 1103 49 1.4787459779111174 1103 50 1.1427083430854879 1104 1 -0.1818075725851216 1104 2 0.19630801097176942 1104 3 -0.014275688607298775 1104 4 -0.036193009934724074 1104 5 0.11345994645067847 1104 6 -0.49598984852403305 1104 7 -0.07449018335269386 1104 8 -0.17956490235272682 1104 9 -0.18438106972944013 1104 10 0.2167448748881391 1104 11 0.0068715587017535995 1104 12 0.2180915126542473 1104 13 0.10050673033038393 1104 14 0.7314305973946382 1104 15 0.20758906854661063 1104 16 0.32024272757964667 1104 17 0.6579658298676812 1104 18 0.1681061657464653 1104 19 0.1689130179806213 1104 20 -0.09090247308167321 1104 21 -0.30756193892709405 1104 22 -0.08468719548660175 1104 23 0.3375258298844379 1104 24 0.17030963081142048 1104 25 0.1633893709987646 1104 26 0.07027332145324294 1104 27 0.05632339303696199 1104 28 0.2070460530589635 1104 29 0.49445801459130934 1104 30 0.5259589512658884 1104 31 0.5398196892015363 1104 32 0.4935170798009594 1104 33 0.2904769874749995 1104 34 0.6142106661933759 1104 35 0.48813302049687124 1104 36 0.5694114252099922 1104 37 0.7085965773468281 1104 38 0.6197682247070162 1104 39 0.07079952826065039 1104 40 0.2621832058835025 1104 41 0.34371296810540386 1104 42 0.2700482374971287 1104 43 0.41355508500082294 1104 44 0.6597311368048082 1104 45 0.5586316970583347 1104 46 0.39737988201896 1104 47 0.5839217263949052 1104 48 0.5719729873601693 1104 49 0.7360062158334628 1104 50 0.7261395517524498 1105 1 0.215652231429722 1105 2 0.5841255354699122 1105 3 0.5812147499808482 1105 4 0.7626605720270305 1105 5 0.29895133642308735 1105 6 0.44072407022775195 1105 7 0.6957172184168812 1105 8 1.0687511474998068 1105 9 1.1072657764317242 1105 10 0.943031622105341 1105 11 0.6503403057286796 1105 12 0.9174921405678624 1105 13 0.8174508840740806 1105 14 1.3725351545252629 1105 15 1.3357452858427727 1105 16 1.2095967376643202 1105 17 0.8560202490475852 1105 18 1.5145337178347518 1105 19 1.234177978498174 1105 20 1.0682926558308985 1105 21 1.0096961716237587 1105 22 0.9506805145314736 1105 23 1.3212191035976915 1105 24 0.9390851992635199 1105 25 1.1701061439033396 1105 26 1.2000135935491407 1105 27 0.9098292440501865 1105 28 0.9528742330608679 1105 29 0.9741412036313619 1105 30 1.1135196020127225 1105 31 1.2846013465129253 1105 32 1.0593481139556573 1105 33 1.1728097816905816 1105 34 0.9108617935837273 1105 35 1.1185202338923617 1105 36 1.3612770897519013 1105 37 1.3669259465790624 1105 38 1.301691923689038 1105 39 1.3317452042191675 1105 40 1.2695278924023867 1105 41 1.658264390424879 1105 42 1.8020718318268791 1105 43 1.7941516029344164 1105 44 1.5569626085962653 1105 45 1.2640973996556149 1105 46 1.4278955322901048 1105 47 1.2798260171379354 1105 48 1.4233334668578408 1105 49 1.218215330094715 1105 50 1.736433529490057 1106 1 0.7778336407250741 1106 2 0.9777537213769598 1106 3 1.0076202972072323 1106 4 0.9489424310974961 1106 5 1.2128006617604186 1106 6 1.2038799922667316 1106 7 0.7416505017752009 1106 8 1.1850742359555788 1106 9 0.4090836175111825 1106 10 0.5771208275026432 1106 11 1.2302690913358738 1106 12 1.3869636548107422 1106 13 1.3695873158581904 1106 14 1.4486408516766893 1106 15 1.815823996487456 1106 16 1.3990645037812612 1106 17 1.4429767707867178 1106 18 0.15409518710012193 1106 19 0.8773563892927783 1106 20 0.5072356843166546 1106 21 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0.4320641191131749 1107 9 0.5768130275995276 1107 10 0.5097761424071727 1107 11 1.0832364609568508 1107 12 0.6695762224938413 1107 13 0.5884174668983704 1107 14 0.682200982768169 1107 15 0.9257021669063796 1107 16 0.8634774419402831 1107 17 0.8024809030181252 1107 18 0.7863087807165385 1107 19 0.7808901595977069 1107 20 0.49188230728616134 1107 21 0.5466706595863897 1107 22 0.7467638778085117 1107 23 1.1505220141258974 1107 24 1.0519849477363898 1107 25 1.1774543293147666 1107 26 0.7709442439264329 1107 27 0.7371379037699128 1107 28 0.5701218076206942 1107 29 0.7215001687768883 1107 30 0.9877380712904795 1107 31 0.9373322124848968 1107 32 0.6991792314547567 1107 33 0.48233188014548745 1107 34 0.4264744418050732 1107 35 0.6530153830120149 1107 36 0.636196241746152 1107 37 0.8190998596225398 1107 38 0.9052312697185023 1107 39 0.9743431201372417 1107 40 0.6803794913962768 1107 41 0.8070247210033905 1107 42 0.8952087344499482 1107 43 0.9397319119534622 1107 44 0.8591904203102452 1107 45 0.9359622936548959 1107 46 0.6108815186357752 1107 47 1.0220286761390156 1107 48 1.228238245873289 1107 49 0.9784560774896107 1107 50 1.0600591133923203 1108 1 -0.4154338449652819 1108 2 -0.7418470045758525 1108 3 -0.2745676826894581 1108 4 -0.7106275814670724 1108 5 -0.5477657908432398 1108 6 -0.7700310785136679 1108 7 -0.3424186731515064 1108 8 -0.8406045997277936 1108 9 -0.4658727555168767 1108 10 -0.7384505113218722 1108 11 -0.6760987014883604 1108 12 -0.7786705068533664 1108 13 -0.3958566322082646 1108 14 -0.7931311014155895 1108 15 -0.6168246842329133 1108 16 -0.15829623892430789 1108 17 -0.7523673587679376 1108 18 -0.49775285143121906 1108 19 -0.5498389199352012 1108 20 -0.42524721391034603 1108 21 -0.4298743265279761 1108 22 -0.2925425095839991 1108 23 -0.9394920388068151 1108 24 -0.6336308718507198 1108 25 -0.5118336554277647 1108 26 -0.5092277372024867 1108 27 -0.8096215568651459 1108 28 -0.8260203277558915 1108 29 -0.674767439213209 1108 30 -0.7876157614796178 1108 31 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-2.1784612732919335 2215 42 -1.975419510997284 2215 43 -2.0631204247685453 2215 44 -1.934688026263295 2215 45 -2.26524080734708 2215 46 -1.954232209148909 2215 47 -1.960901185632359 2215 48 -2.2536384454161116 2215 49 -2.316413373252729 2215 50 -2.1243590830532284 2216 1 -0.9040413207208573 2216 2 -1.8672589243574187 2216 3 -1.907888462277068 2216 4 -2.703330716765066 2216 5 -2.3479258511012198 2216 6 -2.4795217102809204 2216 7 -2.063678441534897 2216 8 -2.314335886800574 2216 9 -2.695370919531566 2216 10 -2.8903665267629783 2216 11 -2.9826611720655194 2216 12 -3.0205027260417694 2216 13 -2.9191595227082168 2216 14 -2.560384655694847 2216 15 -2.8490062113960803 2216 16 -2.9668830009994416 2216 17 -2.4733917444190077 2216 18 -2.273527260620561 2216 19 -2.260883077735784 2216 20 -2.279074537137916 2216 21 -2.359589042656399 2216 22 -2.5745780994531273 2216 23 -4.419249123827058 2216 24 -3.1236977205467014 2216 25 -2.485894478325797 2216 26 -2.5806428581892114 2216 27 -2.613029898829179 2216 28 -2.814863186243791 2216 29 -2.5820266426780414 2216 30 -2.6020400153163585 2216 31 -2.552514621442274 2216 32 -2.598437528107034 2216 33 -2.6975643335773647 2216 34 -3.343528810756318 2216 35 -3.289584300602467 2216 36 -3.3773166724010117 2216 37 -3.7560343085348826 2216 38 -3.6462663869588603 2216 39 -3.6927239565492833 2216 40 -3.5645235970586064 2216 41 -3.4316282750491816 2216 42 -3.4547299104147022 2216 43 -3.7393437692363847 2216 44 -3.913255949387711 2216 45 -3.689161525443552 2216 46 -3.943828042416282 2216 47 -4.1210101032634014 2216 48 -4.1735048313454755 2216 49 -4.722400676749807 2216 50 -4.6692131999180475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25693.0 3 1237.0 4 2975.0 5 1893.0 6 1656.0 7 2136.0 8 2513.0 9 1869.0 10 1554.0 11 1386.0 12 1427.0 13 1430.0 14 1377.0 15 1250.0 16 1185.0 17 1147.0 18 1133.0 19 1484.0 20 2440.0 21 4001.0 22 6052.0 23 9227.0 24 11265.0 25 10050.0 26 7777.0 27 5754.0 28 4886.0 29 4525.0 30 5437.0 31 6644.0 32 8706.0 33 10399.0 34 12249.0 35 20618.0 36 27021.0 37 27464.0 38 37422.0 39 40619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.13443075743802 23.636640070444617 9.809033001909986 15.419896170207375 2 9.387977484312191 37.542723739352724 31.587850144603426 21.481448631731663 3 21.8743565946475 45.14559570228981 21.45054053968445 11.529507163378238 4 9.017816412387855 27.53687465152821 30.99371483602818 32.45159410005575 5 8.94560592571941 54.568956504383145 24.20344632034495 12.2819912495525 6 19.62130162521024 46.69874221207236 15.774453380126888 17.905502782590517 7 19.686346045439286 51.37793654714626 13.998201176835718 14.937516230578726 8 17.356133621768173 42.69321280726704 16.83796444396583 23.11268912699896 9 22.72701358755899 38.19085927849745 15.15377062680138 23.92835650714218 10 17.040397097161463 38.21331618576393 18.18740477238819 26.55888194468642 11 17.333633164730962 42.32690279444974 22.499390307633348 17.840073733185953 12 30.877275232670854 42.36193384481852 15.419947922276215 11.340843000234413 13 27.654843430280067 34.314520471577985 18.855517475119257 19.175118623022687 14 25.70385058854833 36.40510370867808 25.218884228931998 12.672161473841593 15 24.814273902037996 40.09212648066073 20.428761963295138 14.66483765400614 16 12.255953267208799 38.92583006776128 33.89077730646536 14.927439358564563 17 18.631523250987737 42.856780759201705 19.88524210999902 18.626453879811546 18 14.011245743248594 43.47065811356617 29.100182149362475 13.417913993822761 19 17.00603127138187 46.464230506069285 23.67464078457211 12.855097437976736 20 15.353225571389647 42.77359219855909 22.113270433476746 19.759911796574514 21 17.592677664532356 41.96973105180952 23.04799500692251 17.389596276735617 22 13.807304718066012 38.04770337611156 16.254882642345063 31.890109263477363 23 16.261867577319425 44.37341409252038 23.15828821779638 16.20643011236382 24 19.147979222095525 37.55257823387812 21.407893069808694 21.891549474217662 25 16.259840648809618 45.21898908618587 23.39547924157704 15.125691023427477 26 31.158749873263712 33.786943627699486 20.76003751394099 14.294268985095812 27 12.87818786630762 37.967685727863135 29.01346428005703 20.140662125772216 28 20.617658335947574 41.350977092899086 26.594291762126122 11.437072809027217 29 13.853982497164186 31.86029416424276 32.27885401418224 22.006869324410815 30 13.255359388711307 47.5082446041798 28.507164331355472 10.729231675753418 31 20.49445449390327 41.605709922987 21.41411197455498 16.485723608554746 32 15.555309138720357 45.509528410981034 22.488000380185973 16.447162070112633 33 13.561410789696279 41.1432807544994 23.90092918489607 21.394379270908246 34 16.25059789098903 40.62142651246622 24.44400097563139 18.683974620913354 35 16.001064540964233 36.985828298413644 24.3466221418184 22.666485018803723 36 15.54728220402085 38.79437904976157 29.739389426656736 15.918949319560843 37 26.554914760124216 38.20774447049877 21.73870333988212 13.498637429494897 38 23.99858071547147 34.357773750839534 25.402658624054336 16.24098690963466 39 24.696257538134088 33.55768256220544 28.188820757107386 13.557239142553085 40 19.349586800419377 40.03807327680413 25.9506441098867 14.6616958128898 41 13.385572391425532 36.97423098363148 34.154081994628285 15.4861146303147 42 17.526210763042034 42.71863418960438 23.49561306262076 16.259541984732824 43 13.324128845934613 42.975033883491456 28.599566797978394 15.101270472595537 44 16.626660924417646 45.75094683775191 22.43245468478853 15.189937553041913 45 16.50188428286411 41.525160718244294 21.357000348354816 20.615954650536782 46 15.79607386131528 42.53137459188488 23.879524100563366 17.793027446236472 47 14.98533891488971 38.45700787202107 17.948588039341107 28.60906517374811 48 18.35367070247057 42.5124383624116 22.60124082759844 16.53265010751939 49 19.16080992484156 36.19355594758863 22.88737635913889 21.758257768430923 50 16.38544754376631 46.42142332345266 24.110739530288058 13.082389602492968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27631.0 1 21431.0 2 15231.0 3 13157.0 4 11083.0 5 7818.0 6 4553.0 7 3162.5 8 1772.0 9 1520.0 10 1268.0 11 1506.5 12 1745.0 13 2884.0 14 4023.0 15 5375.5 16 6728.0 17 6080.5 18 5433.0 19 4330.5 20 3228.0 21 2877.0 22 2526.0 23 2350.5 24 2175.0 25 2212.5 26 2250.0 27 2341.5 28 2433.0 29 2754.5 30 3076.0 31 3834.0 32 4592.0 33 4987.0 34 5382.0 35 4729.0 36 4076.0 37 4153.5 38 4231.0 39 4340.5 40 4450.0 41 4749.5 42 5049.0 43 7273.5 44 9498.0 45 14502.0 46 19506.0 47 55696.0 48 91886.0 49 60974.0 50 30062.0 51 22625.5 52 15189.0 53 10270.0 54 5351.0 55 4935.0 56 4519.0 57 4478.5 58 4438.0 59 4100.5 60 3763.0 61 3185.5 62 2608.0 63 2221.5 64 1835.0 65 1465.5 66 1096.0 67 921.5 68 747.0 69 668.5 70 590.0 71 543.5 72 497.0 73 453.5 74 410.0 75 353.0 76 296.0 77 267.5 78 239.0 79 204.0 80 169.0 81 137.5 82 106.0 83 80.5 84 55.0 85 48.5 86 42.0 87 30.5 88 19.0 89 16.5 90 14.0 91 12.5 92 11.0 93 8.5 94 6.0 95 4.5 96 3.0 97 3.5 98 4.0 99 5.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06077853504737244 2 0.0664765227080636 3 0.06267786426760281 4 0.07502350419910035 5 0.0823042662099835 6 0.05951231556721885 7 0.04400112693533734 8 0.055080547386681264 9 0.05413088277656608 10 0.0664765227080636 11 0.05318121816645088 12 0.06932551653840918 13 0.06172819965748763 14 0.0661599678380252 15 0.07850560776952273 16 0.07407383958898517 17 0.08863536361075147 18 0.07154140062867798 19 0.06742618731817879 20 0.0845201503002523 21 0.0835704856901371 22 0.07660627854929233 23 0.07280762010883156 24 0.05729643147695005 25 0.07787249802944594 26 0.09021813796094347 27 0.07945527237963793 28 0.10193066815236419 29 0.09338368666132743 30 0.07597316880921555 31 0.07597316880921555 32 0.0842035954302139 33 0.07850560776952273 34 0.0658434129679868 35 0.08610292465044428 36 0.09370024153136584 37 0.1016141132823258 38 0.07882216263956113 39 0.05476399251664288 40 0.06141164478744923 41 0.05476399251664288 42 0.060461980177334035 43 0.03577070031433899 44 0.03577070031433899 45 0.04146868797503015 46 0.037986584404607775 47 0.03133893213380141 48 0.045583901285529325 49 0.05318121816645088 50 0.04210179771510695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 315901.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.349061889642645 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40172873414653 27.7131126523816 2 5.911220615558607 3.706224418409565 3 1.996324420389369 1.877486934197739 4 0.9623152112448501 1.2067071645863736 5 0.5987963486549802 0.9385851896638504 6 0.38674367881088945 0.7274430913482389 7 0.26658049923257127 0.5849933998309598 8 0.18983762824137654 0.4760985245377508 9 0.12824137652475967 0.361822216453889 >10 0.8562888763228048 5.295013311132285 >50 0.12925115114306485 2.8923618475408435 >100 0.11309475725018177 7.846129008771735 >500 0.02625414007593505 5.611884736040722 >1k 0.028273689312545442 21.311740070465113 >5k 0.0020195492366103882 3.734714356713021 >10k+ 0.0030293238549155828 15.715683077926313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26444 8.3709769832954 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12507 3.9591517595702452 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10695 3.3855543350606676 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6441 2.038929917917322 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5357 1.6957844387956986 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4544 1.438425329454481 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4452 1.4093022814109484 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4413 1.396956641479451 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4254 1.3466244171433455 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3941 1.247542742821327 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3679 1.1646053668712666 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3413 1.0804017714410528 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3276 1.0370337542457924 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3163 1.0012630539314533 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3039 0.9620102500466918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2523 0.7986679371068786 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2343 0.7416880604999667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.6691969952611736 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2013 0.6372249533872953 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1953 0.6182316611849915 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1923 0.6087350150838395 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1690 0.5349777303648928 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1626 0.5147182186824353 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1560 0.49382559725990105 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1468 0.4647025492163684 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1462 0.46280321999613805 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1421 0.44982447032456374 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1314 0.4159530992304551 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1283 0.4061398982592648 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1209 0.3827148378764233 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1184 0.3748009661254634 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1058 0.3349150525006252 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1006 0.3184541992586285 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 927 0.293446364525595 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 893 0.2826834989442895 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 882 0.2792013953738671 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 879 0.2782517307637519 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 823 0.26052465804160163 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 809 0.2560928898610641 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 794 0.2513445668104881 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 749 0.23709959765876018 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 722 0.22855261616772346 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 660 0.20892621422534274 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.20860965935530434 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 658 0.20829310448526595 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 640 0.2025951168245748 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 640 0.2025951168245748 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 637 0.20164545221445956 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 613 0.19404813533353804 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 611 0.19341502559346124 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 609 0.19278191585338444 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 603 0.19088258663315405 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 597 0.18898325741292368 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 590 0.18676737332265486 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 576 0.1823356051421173 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 576 0.1823356051421173 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 566 0.1791700564417333 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 514 0.1627092031997366 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 501 0.15859398988923745 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 496 0.15701121553904546 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 480 0.1519463376184311 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 469 0.1484642340480087 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 467 0.1478311243079319 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 462 0.14624834995773994 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 454 0.14371591099743272 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 450 0.14244969151727915 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 445 0.14086691716708716 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 440 0.13928414281689516 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 435 0.13770136846670317 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 434 0.1373848135966648 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 432 0.13675170385658797 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 409 0.12947094184570485 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 400 0.12662194801535925 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 396 0.12535572853520563 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 390 0.12345639931497526 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 390 0.12345639931497526 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 388 0.12282328957489846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 357 0.11301008860370812 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 345 0.10921143016324734 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 337 0.10667899120294017 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 330 0.10446310711267137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 3.1655487003839813E-4 0.0 13 0.0 0.0 0.0 3.1655487003839813E-4 0.0 14 0.0 0.0 0.0 3.1655487003839813E-4 0.0 15 0.0 0.0 0.0 3.1655487003839813E-4 0.0 16 0.0 0.0 0.0 3.1655487003839813E-4 0.0 17 0.0 0.0 0.0 3.1655487003839813E-4 0.0 18 0.0 0.0 0.0 3.1655487003839813E-4 0.0 19 0.0 0.0 0.0 9.496646101151943E-4 0.0 20 0.0 0.0 0.0 0.0012662194801535925 0.0 21 0.0 0.0 0.0 0.0034821035704223793 0.0 22 0.0 0.0 0.0 0.004115213310499175 0.0 23 0.0 0.0 0.0 0.009496646101151944 0.0 24 0.0 0.0 0.0 0.014561524021766312 0.0 25 0.0 0.0 0.0 0.017410517852111895 0.0 26 0.0 0.0 0.0 0.022475395772726267 0.0 27 0.0 0.0 0.0 0.025640944473110248 0.0 28 0.0 0.0 0.0 0.03165548700383981 0.0 29 0.0 0.0 0.0 0.045900456155567725 0.0 30 0.0 0.0 0.0 0.06521030322791001 0.0 31 0.0 0.0 0.0 0.0819877113399451 0.0 32 0.0 0.0 0.0 0.099398229192057 0.0 33 0.0 0.0 0.0 0.12472261879512886 0.0 34 0.0 0.0 0.0 0.15827743501919905 0.0 35 0.0 0.0 0.0 0.19721368403392203 0.0 36 0.0 0.0 0.0 0.23678304278872178 0.0 37 0.0 0.0 0.0 0.27540273693340633 0.0 38 0.0 0.0 0.0 0.3251018515294349 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 705 0.0 32.14439 1 GAAAACA 445 0.0 30.159693 1 TATATCC 30 0.0057364465 29.335657 3 TGGCTTA 30 0.0057364465 29.335657 6 CCAACCA 30 0.00574089 29.331013 38 TACAAAA 65 5.903785E-7 27.07907 2 ACATAGG 50 8.317795E-5 26.40209 3 TACATAG 45 0.0013952849 24.44638 2 TACATGA 55 1.5891295E-4 24.001902 2 CGTCACT 55 1.5891295E-4 24.001902 1 GTACAAA 145 0.0 22.760424 1 CATGAGA 70 3.2036987E-5 22.001743 4 GTCACTA 60 2.864736E-4 22.001743 2 TCACTAC 70 3.2036987E-5 22.001743 3 ACGGCTA 95 6.8489135E-7 20.837154 41 ACATGAG 75 5.4529075E-5 20.53496 3 GAACATA 75 5.4529075E-5 20.53496 1 GCACAAG 65 4.918442E-4 20.309301 28 AGCACAA 55 0.0044743503 20.001585 27 CGGCTAC 100 1.1206102E-6 19.795296 42 >>END_MODULE