##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077439_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 315901 Sequences flagged as poor quality 0 Sequence length 50 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.444550666189723 31.0 30.0 34.0 25.0 34.0 2 29.193905685642022 31.0 30.0 34.0 23.0 34.0 3 29.496595452372738 31.0 30.0 34.0 25.0 34.0 4 32.72720884074441 35.0 33.0 37.0 25.0 37.0 5 32.59490789836056 35.0 33.0 37.0 25.0 37.0 6 32.29629852390464 36.0 33.0 37.0 19.0 37.0 7 32.16229768186869 36.0 33.0 37.0 17.0 37.0 8 32.03219046473421 37.0 33.0 37.0 17.0 37.0 9 33.2168147615867 38.0 33.0 39.0 15.0 39.0 10 33.38351888724632 38.0 33.0 39.0 17.0 39.0 11 33.04787259299591 38.0 33.0 39.0 15.0 39.0 12 33.21009430169578 38.0 33.0 39.0 16.0 39.0 13 32.97531821678311 38.0 33.0 39.0 13.0 39.0 14 33.70173567035242 38.0 32.0 41.0 13.0 41.0 15 33.812966720586516 38.0 33.0 41.0 16.0 41.0 16 33.69726591558748 38.0 33.0 40.0 13.0 41.0 17 34.02236776711691 39.0 33.0 41.0 17.0 41.0 18 34.053877638880536 39.0 33.0 41.0 17.0 41.0 19 34.15550757990636 39.0 34.0 41.0 18.0 41.0 20 34.23775486623974 39.0 34.0 41.0 18.0 41.0 21 34.18663442027724 39.0 34.0 41.0 18.0 41.0 22 34.23643166688298 39.0 34.0 41.0 18.0 41.0 23 34.00669196995261 39.0 33.0 41.0 17.0 41.0 24 33.99004118378859 39.0 33.0 41.0 17.0 41.0 25 33.87061769351791 38.0 33.0 41.0 17.0 41.0 26 33.662207463730724 38.0 33.0 41.0 15.0 41.0 27 33.24184158961194 38.0 32.0 41.0 10.0 41.0 28 33.297270347355656 38.0 33.0 41.0 10.0 41.0 29 33.00540675718025 38.0 32.0 40.0 9.0 41.0 30 32.75052943802014 37.0 32.0 40.0 8.0 41.0 31 32.46732362354029 37.0 32.0 40.0 2.0 41.0 32 32.06526728310452 35.0 31.0 40.0 2.0 41.0 33 31.886296656230908 35.0 31.0 40.0 2.0 41.0 34 31.700779674644906 35.0 31.0 40.0 2.0 41.0 35 31.57191018705227 35.0 31.0 40.0 2.0 41.0 36 31.12045862469571 35.0 30.0 40.0 2.0 41.0 37 31.060329027131917 35.0 30.0 40.0 2.0 41.0 38 30.883238736186335 35.0 30.0 40.0 2.0 41.0 39 30.500555553796918 35.0 30.0 39.0 2.0 41.0 40 30.49192626803967 35.0 30.0 39.0 2.0 41.0 41 30.31312658079588 35.0 30.0 39.0 2.0 41.0 42 30.2019841659254 35.0 29.0 39.0 2.0 41.0 43 30.259508516908777 35.0 29.0 40.0 2.0 41.0 44 30.0882080145362 35.0 28.0 40.0 2.0 41.0 45 29.833498469457204 35.0 27.0 40.0 2.0 41.0 46 29.37889085504636 35.0 25.0 40.0 2.0 41.0 47 29.071120382651525 35.0 24.0 40.0 2.0 41.0 48 28.63851016615965 35.0 23.0 40.0 2.0 41.0 49 28.41359793099737 35.0 23.0 40.0 2.0 41.0 50 28.287504629614975 35.0 23.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4792552880179244 1101 2 0.7614062745129786 1101 3 0.5522753545377128 1101 4 0.5943166114540617 1101 5 0.35904483636000606 1101 6 0.4943061393119734 1101 7 0.7555106452634917 1101 8 0.6494201861062194 1101 9 0.4122998542570855 1101 10 0.7537430845708144 1101 11 0.7930699708279008 1101 12 1.1062270047052536 1101 13 0.7544434036975645 1101 14 0.8329961089267144 1101 15 1.0052010902796056 1101 16 0.6660929997857394 1101 17 1.0910936457889449 1101 18 0.3719097395445843 1101 19 0.5927651090972077 1101 20 0.3962291874234083 1101 21 0.3920456160260315 1101 22 0.4791031486557955 1101 23 0.6190341363382288 1101 24 0.45360206168124506 1101 25 0.4802695491631539 1101 26 0.6879773645867076 1101 27 0.8328812021296415 1101 28 0.8645913537457943 1101 29 0.9565303082777987 1101 30 1.0261094233398893 1101 31 0.9192501135947566 1101 32 0.7290502768546148 1101 33 0.6793616227324151 1101 34 0.8466478645543134 1101 35 1.2174727203434337 1101 36 1.286472398627641 1101 37 1.0541375681256824 1101 38 1.2198360153640948 1101 39 1.287127047967548 1101 40 1.361422998114108 1101 41 1.2902079439126695 1101 42 1.246093682731658 1101 43 1.1313950540502198 1101 44 1.6546714730581726 1101 45 1.661051497953622 1101 46 1.5611710059803023 1101 47 1.8063655545539419 1101 48 1.8241466440044185 1101 49 1.9035283066324098 1101 50 2.079149777528105 1102 1 0.6667334289750251 1102 2 0.5256183996616208 1102 3 0.33192121306099054 1102 4 0.7167422407446793 1102 5 0.5445494180153929 1102 6 0.2688657108247696 1102 7 0.6201594516348408 1102 8 0.7288771824988487 1102 9 0.8991294686385913 1102 10 1.0238808839772418 1102 11 0.8796267843540235 1102 12 1.2896830817689988 1102 13 1.1334067604471372 1102 14 0.9705207159635805 1102 15 1.0234627592612497 1102 16 0.9310495814810977 1102 17 1.050578222048273 1102 18 1.0611416002862413 1102 19 0.7839355546719418 1102 20 1.0444210073948312 1102 21 0.9478199007981445 1102 22 0.9270961137912508 1102 23 1.2527615153515512 1102 24 1.0898026352625294 1102 25 0.9842466458825498 1102 26 0.8929327471574311 1102 27 1.2214675341072976 1102 28 0.9865523222387935 1102 29 1.1308880271430226 1102 30 1.1057163103124026 1102 31 1.0326923007980966 1102 32 1.3970026716064439 1102 33 1.4175977103884208 1102 34 1.3203970276652441 1102 35 1.4613396974501462 1102 36 1.2756702841711949 1102 37 0.9987579952139072 1102 38 1.1916016264679925 1102 39 1.3330585179944308 1102 40 1.4519269302436548 1102 41 1.3507687345355137 1102 42 1.2697014490622358 1102 43 0.8859592222074646 1102 44 1.184289851641804 1102 45 0.8670161437621822 1102 46 0.9430772144481203 1102 47 1.2149323008291617 1102 48 1.6626045697186456 1102 49 1.6225684073588802 1102 50 1.7046831381748326 1103 1 0.5805788496575701 1103 2 0.45534092716086505 1103 3 0.3649737995948712 1103 4 0.4995930202547001 1103 5 0.6141655470162846 1103 6 0.3431744609828087 1103 7 0.3692671241919996 1103 8 0.3323871658680062 1103 9 0.3432167659805714 1103 10 0.7282554767160789 1103 11 0.6494403438409009 1103 12 0.5749166430064889 1103 13 0.5709438394485105 1103 14 0.5615818805116746 1103 15 1.0428897245698892 1103 16 0.7129910136788027 1103 17 0.8103986867393402 1103 18 0.5747963984305926 1103 19 0.4569789991044999 1103 20 0.6321462775244697 1103 21 0.5241418583749038 1103 22 0.3240675495874399 1103 23 0.491127218855695 1103 24 0.3754981643859594 1103 25 0.6846240007030673 1103 26 0.5538629073387185 1103 27 0.5484046383953327 1103 28 0.8135040149863855 1103 29 0.996435921055749 1103 30 0.9458802845978056 1103 31 1.1247308921364407 1103 32 1.3038796714411092 1103 33 1.2741591132146937 1103 34 1.2220562217987379 1103 35 1.3767995743235453 1103 36 1.1980888562527703 1103 37 1.653419980605996 1103 38 1.565771299978266 1103 39 1.3501707810535315 1103 40 1.4491745742074365 1103 41 1.4247759898627343 1103 42 1.6785624793965077 1103 43 1.7687993035138163 1103 44 1.921060550234504 1103 45 1.7699688479763012 1103 46 1.7761414717496464 1103 47 1.8548842561690222 1103 48 2.273734591795847 1103 49 2.220194378707145 1103 50 2.072779149689989 1104 1 0.6232898583177118 1104 2 0.634406489826258 1104 3 0.5526458936721532 1104 4 0.6937663107665628 1104 5 0.533914271387971 1104 6 0.7078067827481718 1104 7 0.33967059799471855 1104 8 0.3520174651472203 1104 9 0.44432645295819384 1104 10 0.3378279280180294 1104 11 0.7001097136537098 1104 12 0.25371660206941726 1104 13 0.4904342704086275 1104 14 0.21496186910778192 1104 15 0.14787868618164168 1104 16 0.037563489348052315 1104 17 0.3331358171482677 1104 18 0.3788248009589452 1104 19 0.6089286270157785 1104 20 0.6621911620965548 1104 21 0.86728709237412 1104 22 0.7285160444191305 1104 23 0.44824563907471315 1104 24 0.6721700781452 1104 25 0.6165027684972841 1104 26 0.47857768805736356 1104 27 0.7171628319009571 1104 28 0.6875737931951917 1104 29 0.47264124969755983 1104 30 0.6088122559613538 1104 31 0.4519301630667343 1104 32 0.46609768082738867 1104 33 0.6591722471910018 1104 34 0.45408168075688593 1104 35 0.3915710333889102 1104 36 0.3904687132654381 1104 37 0.6948297752871042 1104 38 0.5922829256185196 1104 39 0.4071366619559029 1104 40 0.34683677515970857 1104 41 0.5645370928243345 1104 42 0.5115148705599459 1104 43 0.36551069230387867 1104 44 -0.36426107379750405 1104 45 -0.5121655328261632 1104 46 -0.13276867625735278 1104 47 -0.3687442301828483 1104 48 -0.18582786891187553 1104 49 -0.3029521223918188 1104 50 -0.10050161524838686 1105 1 0.483488697628907 1105 2 0.7985916292040542 1105 3 0.708568260716202 1105 4 0.7971770233533775 1105 5 0.7874201224011941 1105 6 0.6945349722639165 1105 7 0.7013916521284784 1105 8 0.6255071426279528 1105 9 0.5267163302296254 1105 10 0.7392121921223023 1105 11 0.7297976596150804 1105 12 0.8350599566503334 1105 13 0.8761825341323473 1105 14 1.0022180768901023 1105 15 0.7349493740324675 1105 16 0.9715849906324223 1105 17 1.0174094352626284 1105 18 0.6886145450365717 1105 19 0.7838406239713507 1105 20 0.6712863956613973 1105 21 0.7817481331885006 1105 22 0.8412312951088836 1105 23 0.7230387129972655 1105 24 0.7196203683174005 1105 25 0.6171116544263171 1105 26 0.42710779936931687 1105 27 0.5039338337085937 1105 28 0.5436531386428172 1105 29 0.7887957545020541 1105 30 0.9495648009142599 1105 31 0.5714240266382333 1105 32 1.181453022850036 1105 33 0.9767300437850466 1105 34 1.2286844778266257 1105 35 1.263010477780501 1105 36 1.0845273185361073 1105 37 1.0874327626336964 1105 38 1.2237261755471636 1105 39 1.033694553688374 1105 40 1.2563886731713154 1105 41 1.2455855182833773 1105 42 1.4892286941733062 1105 43 1.4374591272767638 1105 44 1.3503236469712157 1105 45 1.4878249533311951 1105 46 1.4872579625020421 1105 47 1.2246721907095477 1105 48 1.0831855456062485 1105 49 1.0789287642983147 1105 50 1.1132458873679205 1106 1 0.6842383228163165 1106 2 0.7512715392490321 1106 3 0.6245352772079578 1106 4 0.6714836200550209 1106 5 0.8983459095076398 1106 6 0.9766751521739607 1106 7 1.0600310824133388 1106 8 0.7296300811586889 1106 9 0.9984179794050405 1106 10 1.409995550443135 1106 11 1.1387556806045893 1106 12 0.7673125803384906 1106 13 0.9589036735626308 1106 14 0.8147930394088405 1106 15 1.0533151911239216 1106 16 0.7110902380087296 1106 17 0.9950331234185512 1106 18 1.1751775135523204 1106 19 0.7922739073296725 1106 20 1.1307878949117693 1106 21 1.1155374885108387 1106 22 1.3937050582273258 1106 23 1.19237904283235 1106 24 1.318570893055032 1106 25 1.2910434385342597 1106 26 1.5096602731324396 1106 27 1.3762164424042425 1106 28 1.1111194055093847 1106 29 1.175958423167721 1106 30 1.3460415625334505 1106 31 1.102627175064022 1106 32 1.314117940391263 1106 33 1.175361977818028 1106 34 1.0799588406452187 1106 35 1.1753605527430189 1106 36 1.1748571536185644 1106 37 1.4629200190055087 1106 38 1.2612823974362186 1106 39 1.117277762084175 1106 40 1.7561450449854092 1106 41 1.2642324448201094 1106 42 1.3557954108149843 1106 43 1.4292132502152946 1106 44 1.6651662256818582 1106 45 1.2521802756700282 1106 46 1.240425128918833 1106 47 1.1878655940078993 1106 48 0.9393324721429792 1106 49 1.1996746413597847 1106 50 1.6676499353439311 1107 1 0.6010237317647338 1107 2 0.8817752440082209 1107 3 0.8657008514456308 1107 4 0.7064116622322842 1107 5 0.6052246498895144 1107 6 1.1955231145564085 1107 7 1.1176151519488187 1107 8 1.15624735103615 1107 9 0.913529122180762 1107 10 1.0376850631248118 1107 11 0.8817380117530647 1107 12 1.059008932575594 1107 13 1.0689421460619215 1107 14 1.0728972001383923 1107 15 1.1385246525089272 1107 16 0.9587391408300476 1107 17 1.3205175919280947 1107 18 1.359257735837872 1107 19 1.489374220809296 1107 20 1.419741300440407 1107 21 1.2657761604971611 1107 22 1.5769481474265277 1107 23 1.6396574697346011 1107 24 1.361104378378485 1107 25 1.4378267101216053 1107 26 1.3337732682877501 1107 27 1.2816916490158121 1107 28 1.5494921612802628 1107 29 1.4785909395541523 1107 30 1.0792989008064637 1107 31 1.036030566343591 1107 32 0.9176908956491729 1107 33 0.8114228213631947 1107 34 0.7223783333781881 1107 35 0.8642142147549912 1107 36 0.703284055869922 1107 37 0.9352768158137152 1107 38 0.8777685758346259 1107 39 0.9669500333865422 1107 40 0.5876319358375035 1107 41 0.7454058560483787 1107 42 0.8539430312196572 1107 43 0.8197803633529404 1107 44 0.9650379840175667 1107 45 0.7832076338917418 1107 46 0.5695252999077205 1107 47 0.7650243286901457 1107 48 1.0511877015530686 1107 49 1.1970207513626328 1107 50 1.2133716509324195 1108 1 0.3281375270603455 1108 2 0.6414450000894618 1108 3 0.5457624166746591 1108 4 0.35844264560297745 1108 5 0.30157256035226254 1108 6 0.5897998904121309 1108 7 0.7023509902731746 1108 8 0.5185453401283979 1108 9 0.24410763457745333 1108 10 0.37194410551782653 1108 11 0.3966608879199569 1108 12 -0.08132800321588718 1108 13 0.18236102383486497 1108 14 0.0443145387806112 1108 15 0.33137605947516846 1108 16 -0.11017878335103148 1108 17 0.06143337470990673 1108 18 0.27668002646590395 1108 19 0.3774861089211825 1108 20 0.44005949898827623 1108 21 -0.0017230866671553713 1108 22 -0.026265624447532332 1108 23 0.05214450423120809 1108 24 -0.011632932157866094 1108 25 -0.003360093505385464 1108 26 0.1965145939477395 1108 27 0.1846678594082931 1108 28 0.06913280771516384 1108 29 0.26343050190568107 1108 30 0.38115254955006606 1108 31 0.14949126832103588 1108 32 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-2.2033399134319147 2216 24 -2.3920114275914415 2216 25 -2.7457101547048985 2216 26 -2.6412596269354154 2216 27 -2.447240623785383 2216 28 -2.4822277044776584 2216 29 -2.325826630191113 2216 30 -2.3332811195959664 2216 31 -2.5303882157200164 2216 32 -2.3708297802922225 2216 33 -2.3540586316446834 2216 34 -2.6214321901363498 2216 35 -2.6955257281773406 2216 36 -2.881308412972018 2216 37 -2.9521993443897188 2216 38 -3.2668003858590744 2216 39 -2.8533903028100305 2216 40 -2.589850729488713 2216 41 -3.019367425638219 2216 42 -2.863361334604793 2216 43 -2.065127012569235 2216 44 -2.5823200490698532 2216 45 -2.775401304738338 2216 46 -2.951701359268199 2216 47 -3.1343384649644044 2216 48 -3.0384758061865504 2216 49 -3.1283240940667802 2216 50 -3.2596949230855294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12954.0 3 1858.0 4 3310.0 5 1723.0 6 1247.0 7 898.0 8 822.0 9 806.0 10 834.0 11 982.0 12 1119.0 13 1268.0 14 1396.0 15 1355.0 16 1430.0 17 1353.0 18 1403.0 19 1578.0 20 1924.0 21 2914.0 22 4024.0 23 4452.0 24 4292.0 25 4257.0 26 3930.0 27 4403.0 28 5172.0 29 5968.0 30 6762.0 31 8153.0 32 9909.0 33 12545.0 34 16362.0 35 26061.0 36 34033.0 37 30950.0 38 35855.0 39 57599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.11177951210245 16.22251420791844 15.343602083300354 17.32210419667875 2 10.31978676661454 26.563954187744145 38.360166889312374 24.75609215632894 3 22.767892472641744 34.174314104735345 29.771668972241304 13.286124450381607 4 9.319294824513364 17.460026021469293 36.72667643790778 36.49400271610957 5 9.21018878321758 44.204915774372225 31.270050893244534 15.31484454916566 6 22.6681907560391 39.020065417631095 18.963197041324563 19.34854678500524 7 19.445869351825465 41.111427757195784 20.149140305880117 19.293562585098638 8 22.155379583514073 29.55485968850627 22.116118328357075 26.173642399622587 9 24.91870034546416 22.511217294106846 21.933016050942506 30.63706630948649 10 18.65834929782619 26.643340035781577 24.96477256534887 29.733538101043365 11 20.914295847794694 28.402325640311098 28.55591163580169 22.12746687609252 12 33.72727474246555 30.70937954228062 23.128224128594635 12.435121586659195 13 33.18534609696858 20.47068807731647 26.79983406851957 19.54413175719538 14 28.721760223954806 21.787458277650753 36.03037577031966 13.460405728074786 15 26.673422210962983 26.112289812850314 31.3866810221983 15.82760695398841 16 12.951597375288188 25.126675280585747 43.438853842061256 18.482873502064805 17 18.374650063970208 28.37965367416997 32.09214243188123 21.15355382997859 18 14.06221794331789 26.06274999129088 44.35348253900893 15.521549526382294 19 17.194234807146003 29.645265188103508 40.653413859391875 12.507086145358615 20 14.506495398432998 25.75515736535745 39.60704581298573 20.13130142322382 21 15.966101802266847 25.854976264294464 40.304779759887765 17.87414217355092 22 12.917037454594974 22.201356054584206 33.92543283222862 30.9561736585922 23 14.67356193709385 27.097716073622742 40.930481486642265 17.298240502641146 24 18.265421484148625 21.218454795631096 38.944571643913264 21.571552076307015 25 15.63858725770545 28.26909618313842 40.94140596548896 15.150910593667168 26 28.980669841913254 17.680254357519253 38.73030249290637 14.608773307661128 27 12.856224487533924 21.40814562175206 46.201274950360535 19.534354940353477 28 19.201525126909182 24.097701859223438 44.580827609357236 12.119945404510144 29 13.244347330419911 15.03578440686554 49.57787066945342 22.14199759326113 30 12.851741369155063 29.812277935057285 46.99986699853698 10.336113697250669 31 19.454005972304465 25.07639546408859 39.192693901979474 16.27690466162747 32 14.927693637610073 28.240170482791815 39.674613453068154 17.157522426529958 33 13.277331678403161 23.654947059121536 42.12947717716196 20.93824408531334 34 14.79511388894166 24.31150541107037 41.876745378901575 19.016635321086394 35 14.904981730909276 20.03045903860889 42.88518652203373 22.17937270844811 36 16.022645237281054 21.540205428271083 46.619995693858684 15.817153640589183 37 24.710851346088695 21.78849484392463 39.268492690942594 14.232161119044076 38 23.21411609749302 17.900315984777006 42.354941457329396 16.530626460400587 39 23.51207342516756 16.938570691352787 45.15400859244156 14.39534729103809 40 18.089750692520777 22.77958053027305 43.75464978235061 15.376018994855558 41 13.286691148537418 19.908192984677726 50.086108648854 16.71900721793086 42 17.300873749525138 25.698366468279094 40.933582373053056 16.06717740914271 43 13.415217081625224 25.729435413665936 45.216606783899714 15.63874072080913 44 16.674686437512268 28.694845608858895 38.89662726426622 15.73384068936262 45 16.40534234910143 24.729732724262483 37.719023333998116 21.145901592637966 46 15.616017727128838 25.510921177587843 39.591959480848374 19.28110161443495 47 14.593542260208928 22.51630262741374 33.82842671731561 29.06172839506173 48 17.774724752926577 25.8877865640175 37.756491019253055 18.580997663802872 49 18.847676962086226 20.36840655775069 37.798867366675005 22.98504911348809 50 16.358024691358025 29.556505223171893 39.551123773346 14.534346312124091 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10499.0 1 6499.0 2 2499.0 3 3956.0 4 5413.0 5 5201.0 6 4989.0 7 3386.5 8 1784.0 9 2178.5 10 2573.0 11 3976.0 12 5379.0 13 9117.5 14 12856.0 15 14641.0 16 16426.0 17 14437.5 18 12449.0 19 9621.5 20 6794.0 21 5734.0 22 4674.0 23 4047.5 24 3421.0 25 3122.5 26 2824.0 27 2855.0 28 2886.0 29 2961.5 30 3037.0 31 3673.0 32 4309.0 33 4652.5 34 4996.0 35 4331.5 36 3667.0 37 3640.5 38 3614.0 39 3673.5 40 3733.0 41 4041.5 42 4350.0 43 6513.0 44 8676.0 45 13995.5 46 19315.0 47 56585.0 48 93855.0 49 61868.5 50 29882.0 51 22288.5 52 14695.0 53 9805.0 54 4915.0 55 5037.5 56 5160.0 57 4821.0 58 4482.0 59 4139.5 60 3797.0 61 3113.0 62 2429.0 63 2083.0 64 1737.0 65 1398.0 66 1059.0 67 831.5 68 604.0 69 571.0 70 538.0 71 496.5 72 455.0 73 401.0 74 347.0 75 319.5 76 292.0 77 246.5 78 201.0 79 159.5 80 118.0 81 99.0 82 80.0 83 62.0 84 44.0 85 36.5 86 29.0 87 20.5 88 12.0 89 8.5 90 5.0 91 2.5 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017727072722150294 2 9.496646101151944E-4 3 0.0 4 0.0025324389603071846 5 0.044950791545452534 6 0.02659060908322544 7 0.02880649317349423 8 0.02152573116261107 9 0.029756157783609426 10 0.03038926752368622 11 0.037036919794492584 12 0.037353474664530976 13 0.03418792596414699 14 0.03893624901472297 15 0.03513759057426219 16 0.04210179771510695 17 0.04083557823495336 18 0.044317681805375736 19 0.045583901285529325 20 0.04400112693533734 21 0.04178524284506855 22 0.04178524284506855 23 0.04083557823495336 24 0.039252803884761364 25 0.037353474664530976 26 0.03956935875479976 27 0.039252803884761364 28 0.039252803884761364 29 0.03830313927464617 30 0.03640381005441578 31 0.03482103570422379 32 0.029123048043532624 33 0.024374724992956655 34 0.021209176292572674 35 0.021209176292572674 36 0.023108505512803062 37 0.018993292202303887 38 0.01994295681241908 39 0.01329530454161272 40 0.00823042662099835 41 0.00664765227080636 42 0.00664765227080636 43 0.0018993292202303888 44 0.004748323050575972 45 0.003165548700383981 46 3.165548700383981E-4 47 3.165548700383981E-4 48 9.496646101151944E-4 49 0.0 50 3.165548700383981E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 315901.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.500698003488434 #Duplication Level Percentage of deduplicated Percentage of total 1 86.57946412284184 25.54154624391819 2 6.555213374395072 3.867667402129148 3 2.362838410610239 2.091161471473658 4 1.2168295902052728 1.4358928904941737 5 0.7114268239030828 1.0493793941772898 6 0.44960458403528164 0.7958189432765328 7 0.2940134988679407 0.6071522407336476 8 0.22319272906763382 0.5267473037438944 9 0.1641754209007114 0.4358960560428742 >10 1.1041601837047847 6.32160075466681 >50 0.14700674943397035 3.0506392825600424 >100 0.12661895206721535 7.850560776952273 >500 0.027899091133454228 5.3836486747430365 >1k 0.030045175066796864 21.803982893374823 >5k 0.0042921678666852665 8.521023991693601 >10k+ 0.0032191259000139494 10.717281680020006 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11943 3.780614812868589 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11639 3.6843821323769155 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10274 3.252284734774502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8027 2.5409859417982217 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6823 2.1598538782719903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6590 2.0860965935530436 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5478 1.7340875780703449 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4902 1.5517519729282276 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4655 1.4735629200287432 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4478 1.4175327080319469 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4419 1.3988559706996813 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4378 1.385877221028107 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4277 1.3539051791542287 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3677 1.16397225713119 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3206 1.0148749133431043 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3162 1.000946499061415 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3052 0.966125463357191 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.9626433597867686 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2597 0.82209299748972 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2202 0.6970538238245527 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2111 0.6682473306510583 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1954 0.6185482160550299 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1747 0.5530213579570815 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1730 0.5476399251664288 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1574 0.49825736544043864 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1376 0.4355795011728358 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1358 0.42988151351214465 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1356 0.4292484037720678 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.3842976122266153 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1177 0.3725850820351946 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1109 0.3510593508725835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1070 0.338713710941086 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1035 0.327634290489742 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1016 0.32161974795901244 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1006 0.3184541992586285 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 969 0.30674166906720773 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 947 0.299777461926363 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 888 0.2811007245940975 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 885 0.2801510599839823 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 841 0.2662226457022928 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 744 0.23551682330856819 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 686 0.2171566408463411 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 669 0.21177520805568834 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 662 0.20955932396541954 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 662 0.20955932396541954 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 655 0.20734343987515078 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 633 0.20037923273430602 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 632 0.2000626778642676 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 598 0.18929981228296208 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 595 0.18835014767284686 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 589 0.1864508184526165 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 584 0.1848680441024245 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 560 0.17727072722150294 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 553 0.17505484313123415 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 547 0.17315551391100376 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 540 0.170939629820735 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 534 0.1690403006005046 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 512 0.16207609345965981 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 511 0.16175953858962144 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 507 0.16049331910946785 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 504 0.15954365449935265 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 495 0.15669466066900706 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 493 0.15606155092893026 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 474 0.1500470083982007 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 457 0.14466557560754795 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 449 0.14213313664724073 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 443 0.14023380742701036 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 396 0.12535572853520563 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 395 0.12503917366516726 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 395 0.12503917366516726 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.11934118600447607 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.1190246311344377 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 376 0.1190246311344377 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 373 0.11807496652432249 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 372 0.11775841165428409 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.1164921921741305 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 358 0.11332664347374652 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 352 0.11142731425351612 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 352 0.11142731425351612 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 351 0.11111075938347774 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 347 0.10984453990332413 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.10731210094301695 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 327 0.10351344250255619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 3.1655487003839813E-4 0.0 13 0.0 0.0 0.0 3.1655487003839813E-4 0.0 14 0.0 0.0 0.0 3.1655487003839813E-4 0.0 15 0.0 0.0 0.0 3.1655487003839813E-4 0.0 16 0.0 0.0 0.0 6.331097400767963E-4 0.0 17 0.0 0.0 0.0 6.331097400767963E-4 0.0 18 0.0 0.0 0.0 6.331097400767963E-4 0.0 19 0.0 0.0 0.0 0.0012662194801535925 0.0 20 0.0 0.0 0.0 0.0015827743501919905 0.0 21 0.0 0.0 0.0 0.004115213310499175 0.0 22 0.0 0.0 0.0 0.0053814327906527674 0.0 23 0.0 0.0 0.0 0.010762865581305535 0.0 24 0.0 0.0 0.0 0.015511188631881508 0.0 25 0.0 0.0 0.0 0.017727072722150294 0.0 26 0.0 0.0 0.0 0.022158840902687868 0.0 27 0.0 0.0 0.0 0.02532438960307185 0.0 28 0.0 0.0 0.0 0.031338932133801414 0.0 29 0.0 0.0 0.0 0.045583901285529325 0.0 30 0.0 0.0 0.0 0.06521030322791001 0.0 31 0.0 0.0 0.0 0.0829373759500603 0.0 32 0.0 0.0 0.0 0.100981003542249 0.0 33 0.0 0.0 0.0 0.12820472236555122 0.0 34 0.0 0.0 0.0 0.16175953858962144 0.0 35 0.0 0.0 0.0 0.200379232734306 0.0 36 0.0 0.0 0.0 0.24343069505952813 0.0 37 0.0 0.0 0.0 0.2855324927746351 0.0 38 0.0 0.0 0.0 0.335548162240702 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTAGG 20 7.834445E-4 44.01521 1 AGCGGTG 20 7.8466826E-4 44.001266 8 GTACCAG 40 4.100748E-7 38.51331 1 GTTATCA 30 1.2963176E-4 36.679344 1 ATCTAAA 25 0.0023461853 35.21217 18 AATCTAA 25 0.0023461853 35.21217 17 TACATAG 25 0.00234801 35.206593 2 AAGCGGT 25 0.002349836 35.201015 7 GCTTATG 45 1.0312015E-6 34.234055 1 CTTATGT 45 1.0324802E-6 34.22863 2 GAACAAA 210 0.0 33.5354 1 GTACATA 40 1.8115456E-5 33.01141 1 TACCAGC 35 3.2089936E-4 31.434454 2 TGTAGGA 30 0.005733419 29.338825 2 ACAAGCG 30 0.005733419 29.338825 5 GCGGTGG 30 0.0057378607 29.334179 9 AAGCATG 30 0.0057378607 29.334179 31 TAACAAC 70 3.5610356E-8 28.295492 1 TTATGTA 55 4.9386217E-6 28.005243 3 TTGGTAT 40 7.0063747E-4 27.509506 17 >>END_MODULE