FastQCFastQC Report
Mon 6 Feb 2017
SRR2077438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798366
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.024.475171
GTATCAA38100.024.1374741
TATCAAC50150.017.9861372
TCAACGC49550.017.9822544
ATCAACG50200.017.9231153
CAACGCA50550.017.582375
AACGCAG52150.017.042326
CGTGCCG2600.016.07787110
CGCACGA2353.6379788E-1215.91554635
ACCGTGC6050.014.909178
GTGCCGA3300.014.66674511
AATAGCG1057.163667E-414.6664825
ACGCAGA60800.014.29258257
GTACATG69200.014.1161861
TGGACCG3500.013.8283975
CGCAGAG62700.013.7895478
TACATGG71400.013.4958712
GCACCGT4250.013.4581726
AGAGTAC61450.013.38981811
ACCGCGA1150.001428472913.38658739
Produced by FastQC (version 0.11.5)