FastQCFastQC Report
Mon 6 Feb 2017
SRR2077438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798366
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.027.4573081
GTATCAA41350.025.222831
TATCAAC58750.017.5275482
ATCAACG59600.017.1302093
TCAACGC60150.016.9372984
CAACGCA60850.016.7068925
AACGCAG63200.016.1178936
TCGATCG900.00437086614.661633541
GTACATG65100.014.229581
CGCTACG5450.014.12939928
TATACAC10650.014.0491573
CTAATAC3850.013.7164413
TACATGG66200.013.6605142
ACGCAGA74700.013.5479597
CGTGCCG2451.5352271E-913.47030210
CGCAGAG77050.013.1633048
CCTATAC3350.013.1363943
AGAGTAC74900.012.95438111
TATCTCG5500.012.79903836
ACATGGG69900.012.7802653
Produced by FastQC (version 0.11.5)