##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077438_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2798366 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.531753887804527 31.0 31.0 34.0 27.0 34.0 2 30.485005178021744 31.0 31.0 34.0 26.0 34.0 3 30.681417655874892 31.0 31.0 34.0 27.0 34.0 4 34.15634981271213 37.0 35.0 37.0 30.0 37.0 5 33.77136193049801 37.0 35.0 37.0 28.0 37.0 6 33.72143672414545 37.0 35.0 37.0 28.0 37.0 7 33.55149183487792 37.0 35.0 37.0 28.0 37.0 8 33.51726900627009 37.0 35.0 37.0 28.0 37.0 9 34.98733510913154 39.0 35.0 39.0 28.0 39.0 10 34.83775031571996 39.0 35.0 39.0 27.0 39.0 11 34.79682428960329 39.0 35.0 39.0 27.0 39.0 12 34.71676078111298 39.0 35.0 39.0 27.0 39.0 13 34.684240017210044 39.0 35.0 39.0 27.0 39.0 14 35.854527606467485 40.0 36.0 41.0 27.0 41.0 15 35.858542449415125 40.0 36.0 41.0 27.0 41.0 16 35.795091492678225 40.0 36.0 41.0 27.0 41.0 17 35.75531935422314 40.0 36.0 41.0 27.0 41.0 18 35.74898387130204 40.0 36.0 41.0 27.0 41.0 19 35.77908357948889 40.0 36.0 41.0 27.0 41.0 20 35.77546932745752 40.0 36.0 41.0 27.0 41.0 21 35.71827237752317 40.0 36.0 41.0 27.0 41.0 22 35.71632159624581 40.0 36.0 41.0 27.0 41.0 23 35.643112802256745 40.0 36.0 41.0 26.0 41.0 24 35.63165468705666 40.0 36.0 41.0 26.0 41.0 25 35.60578566206136 40.0 36.0 41.0 26.0 41.0 26 35.526427565229135 40.0 36.0 41.0 26.0 41.0 27 35.471477998231826 40.0 35.0 41.0 25.0 41.0 28 35.40812138226379 40.0 35.0 41.0 25.0 41.0 29 35.33073157692739 40.0 35.0 41.0 25.0 41.0 30 35.206020227518486 40.0 35.0 41.0 24.0 41.0 31 35.13072450136973 39.0 35.0 41.0 24.0 41.0 32 35.04968649561923 39.0 35.0 41.0 24.0 41.0 33 34.95501481936244 39.0 35.0 41.0 24.0 41.0 34 34.924892598037566 39.0 35.0 41.0 24.0 41.0 35 34.864543808779835 39.0 35.0 41.0 24.0 41.0 36 34.757389848218565 39.0 35.0 41.0 23.0 41.0 37 34.66990200709986 39.0 34.0 41.0 22.0 41.0 38 34.59183144735177 39.0 34.0 41.0 22.0 41.0 39 34.50333658999573 39.0 34.0 41.0 21.0 41.0 40 34.41054208062848 39.0 34.0 41.0 19.0 41.0 41 34.352524651886135 39.0 34.0 41.0 19.0 41.0 42 34.24243576429959 39.0 34.0 41.0 18.0 41.0 43 34.34443814711871 39.0 34.0 41.0 18.0 41.0 44 34.29210903791713 39.0 34.0 41.0 16.0 41.0 45 34.22431912051533 39.0 34.0 41.0 15.0 41.0 46 34.08038298063941 39.0 34.0 41.0 12.0 41.0 47 33.932892981118265 39.0 34.0 41.0 8.0 41.0 48 33.78381241052814 39.0 34.0 41.0 2.0 41.0 49 33.630663036929406 39.0 33.0 41.0 2.0 41.0 50 33.484907978441704 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7163110107057022 1101 2 0.8955652236953 1101 3 0.755247447194975 1101 4 0.8190151993318082 1101 5 0.8668557134203994 1101 6 0.8658597174461988 1101 7 0.8791155102574066 1101 8 0.8744752252461154 1101 9 0.9322513358220732 1101 10 0.9540332697653966 1101 11 1.060592817582787 1101 12 1.0511481571231656 1101 13 0.9854394918717446 1101 14 1.1908358183431815 1101 15 1.1941735644728055 1101 16 1.3160954203621529 1101 17 1.2734581253944555 1101 18 1.2062593443213743 1101 19 1.2680319186237554 1101 20 1.301394414248513 1101 21 1.2962229018309017 1101 22 1.339685683146854 1101 23 1.377627606707243 1101 24 1.281185345821008 1101 25 1.3251391002890998 1101 26 1.2559046155457025 1101 27 1.2054637602110247 1101 28 1.446223022104391 1101 29 1.4767886530684464 1101 30 1.4771747370630166 1101 31 1.4535868318398855 1101 32 1.4246144206698759 1101 33 1.5388177606489606 1101 34 1.3772954340505308 1101 35 1.3988508771119754 1101 36 1.2863204598102698 1101 37 1.4558313704471146 1101 38 1.5112456551840268 1101 39 1.5725601713800472 1101 40 1.6094415471263304 1101 41 1.5564425679904943 1101 42 1.501909092329484 1101 43 1.5602154203459122 1101 44 1.577707277189802 1101 45 1.5145826766770796 1101 46 1.5652776073818018 1101 47 1.578664502726106 1101 48 1.6781619744140173 1101 49 1.7518592518434204 1101 50 1.7064298956999764 1102 1 0.5289776213130182 1102 2 0.6436717453157712 1102 3 0.5532163652347286 1102 4 0.5897549467401717 1102 5 0.6027462752383741 1102 6 0.5550561582674405 1102 7 0.5233143631060386 1102 8 0.6003304254148887 1102 9 0.6548783286951618 1102 10 0.6850332063298126 1102 11 0.8232447126101761 1102 12 0.781827780876398 1102 13 0.8002855941977671 1102 14 0.803886693680461 1102 15 0.841094430796332 1102 16 0.8097726999493204 1102 17 0.8988944294308823 1102 18 0.8718822743666834 1102 19 0.900614683618528 1102 20 0.88176065884727 1102 21 0.9487845560538659 1102 22 0.8553893091542619 1102 23 0.7966718168826077 1102 24 0.8182296976199979 1102 25 0.7886064744837995 1102 26 0.7701355771258704 1102 27 0.7804389023141027 1102 28 0.8026504112285835 1102 29 0.8890258932960435 1102 30 0.9796492780033077 1102 31 0.9255184305788475 1102 32 0.9457235328225124 1102 33 1.0312104696876432 1102 34 0.9408776192735857 1102 35 1.0034860266207914 1102 36 0.9009373088911161 1102 37 0.8888994545302467 1102 38 0.9435195036437989 1102 39 0.9301970779069748 1102 40 0.9350296539872573 1102 41 0.8916579465460259 1102 42 0.8469298785471437 1102 43 0.8679443822299788 1102 44 0.8705377286298628 1102 45 0.8261491224994018 1102 46 0.9493059291565871 1102 47 0.9362197174998101 1102 48 0.9001494480993131 1102 49 0.9140068708699616 1102 50 0.8978014025015995 1103 1 0.44623978339800274 1103 2 0.6460550551081923 1103 3 0.553094842333639 1103 4 0.5185053393653902 1103 5 0.5087904790276312 1103 6 0.5120908401065378 1103 7 0.595311701555282 1103 8 0.5863825182318223 1103 9 0.6174997436865723 1103 10 0.6289898990209792 1103 11 0.6181651881565102 1103 12 0.696455618651953 1103 13 0.7078431897443167 1103 14 0.707084074098276 1103 15 0.7817899269451161 1103 16 0.7882213107034985 1103 17 0.8989495463750927 1103 18 0.7470809111852006 1103 19 0.6655349400252106 1103 20 0.6960092522030905 1103 21 0.7864890614988198 1103 22 0.7050834101138932 1103 23 0.8010519537984067 1103 24 0.7235029282498786 1103 25 0.7809511957746764 1103 26 0.6884602345209316 1103 27 0.7264681249194851 1103 28 0.7949645857792973 1103 29 0.8001992931947086 1103 30 0.9128076980462154 1103 31 0.7890468845352814 1103 32 0.9012242370576828 1103 33 0.7959222113897226 1103 34 0.8600363354870595 1103 35 0.9322583332117773 1103 36 0.8764501122916357 1103 37 0.8456200361933028 1103 38 0.827305094746464 1103 39 0.8104359268526835 1103 40 0.7946760157044892 1103 41 0.8564202895872413 1103 42 0.8185898083294845 1103 43 0.7593892363991728 1103 44 0.6867526023688981 1103 45 0.7446988960882948 1103 46 0.7522429318174702 1103 47 0.7952305742097039 1103 48 0.8590693950542345 1103 49 0.8327625029251848 1103 50 0.8752452911281878 1104 1 0.40826084626487 1104 2 0.6038610747229285 1104 3 0.46930420226453506 1104 4 0.46868463307103525 1104 5 0.5152771608558879 1104 6 0.4679170568306077 1104 7 0.4495946989742592 1104 8 0.42630447006008154 1104 9 0.39754338254608257 1104 10 0.44489552884983397 1104 11 0.5021166303382714 1104 12 0.4896611119225085 1104 13 0.6076090062310158 1104 14 0.6193663266537897 1104 15 0.6160916044478313 1104 16 0.5479436963204876 1104 17 0.5904545280557372 1104 18 0.6371927511994429 1104 19 0.5117934310462218 1104 20 0.5848699035881424 1104 21 0.5968888113910893 1104 22 0.6268883455818113 1104 23 0.6735711067339523 1104 24 0.6780513076518702 1104 25 0.6692515746410947 1104 26 0.6210474911159949 1104 27 0.6548013401752826 1104 28 0.6725651894696867 1104 29 0.6883976270664363 1104 30 0.6914597551628177 1104 31 0.6680403699048227 1104 32 0.5812657310224054 1104 33 0.6685759077259164 1104 34 0.6257936750895539 1104 35 0.7014072064595496 1104 36 0.6482898535523276 1104 37 0.8265668707510656 1104 38 0.7867594335111505 1104 39 0.7090779484615197 1104 40 0.715079619907705 1104 41 0.6892900873236627 1104 42 0.680153098650095 1104 43 0.7080567042598744 1104 44 0.7173888496609706 1104 45 0.6029531987147507 1104 46 0.6139173666864437 1104 47 0.698817553268384 1104 48 0.6458473535953715 1104 49 0.7232103764569828 1104 50 0.6725286843777454 1105 1 0.4088680807198486 1105 2 0.5769508464540678 1105 3 0.5034963099263301 1105 4 0.5139126991216685 1105 5 0.5582062386369131 1105 6 0.5720659701798496 1105 7 0.6438802349123094 1105 8 0.6048219906731518 1105 9 0.6693479847796908 1105 10 0.6437138657468822 1105 11 0.722084787248022 1105 12 0.7493169515827773 1105 13 0.8634107086102887 1105 14 0.7692640795937038 1105 15 0.8066263890164365 1105 16 0.7944700415694186 1105 17 0.8865914233951742 1105 18 0.7565613099831978 1105 19 0.7532102058750922 1105 20 0.8074045210869443 1105 21 0.8735220864737485 1105 22 0.7976452102026599 1105 23 0.8410994852200204 1105 24 0.7520268533840451 1105 25 0.7497085908582832 1105 26 0.7356617322900831 1105 27 0.843979198997971 1105 28 0.8359309367533285 1105 29 0.8195495979880292 1105 30 0.9077267308627128 1105 31 0.9677024264892751 1105 32 0.9935612878206541 1105 33 0.8079452800569698 1105 34 0.7119122461080707 1105 35 0.7801179646357141 1105 36 0.842232012864045 1105 37 0.8818508518269681 1105 38 0.8209120112655839 1105 39 0.6956533853803109 1105 40 0.8127492429409813 1105 41 0.8738146047877109 1105 42 0.9731769420689744 1105 43 0.9736269983319801 1105 44 0.9244613066042078 1105 45 0.9338928785431548 1105 46 0.924812285349212 1105 47 0.9186538673231084 1105 48 0.7870347000548108 1105 49 1.0012779732870456 1105 50 1.06452847370457 1106 1 0.4080736446719513 1106 2 0.5116772676438259 1106 3 0.35663485449571297 1106 4 0.42964626034776643 1106 5 0.3235332409243412 1106 6 0.4589628527585745 1106 7 0.4013940328293586 1106 8 0.41710715727279535 1106 9 0.38028947621185694 1106 10 0.454527261935155 1106 11 0.4214637094670053 1106 12 0.39048728181564485 1106 13 0.45061916055212947 1106 14 0.45033741985648135 1106 15 0.49504497132056 1106 16 0.5223459475589891 1106 17 0.5109801045507822 1106 18 0.5093452108791752 1106 19 0.4688152107833119 1106 20 0.42851491058947744 1106 21 0.5618907839047154 1106 22 0.5816513199330515 1106 23 0.6241087863046175 1106 24 0.5915841023588371 1106 25 0.5273194931806486 1106 26 0.662150261074629 1106 27 0.5834543934670435 1106 28 0.5893340100281534 1106 29 0.6314929032186214 1106 30 0.6491159934715114 1106 31 0.6313889441690463 1106 32 0.6580453524090117 1106 33 0.6585389183801951 1106 34 0.6558546476336389 1106 35 0.5962059917717468 1106 36 0.6476014584014607 1106 37 0.597158102926592 1106 38 0.5979810683176012 1106 39 0.576887612691678 1106 40 0.6617989499951165 1106 41 0.7492100798964074 1106 42 0.8394488738451358 1106 43 0.722474604988534 1106 44 0.7446906114784966 1106 45 0.6698946304021263 1106 46 0.7645378541075942 1106 47 0.7325564037244874 1106 48 0.7721265668262021 1106 49 0.7804869136340145 1106 50 0.7889616568333295 1107 1 0.27118433880268 1107 2 0.4417401516954378 1107 3 0.34101279942422735 1107 4 0.34747942743413773 1107 5 0.4210931482826865 1107 6 0.4306536134341954 1107 7 0.44778247815518313 1107 8 0.40500971339253056 1107 9 0.44263219610433424 1107 10 0.5169990166918552 1107 11 0.5090777236521689 1107 12 0.4388378693770747 1107 13 0.46676844187019384 1107 14 0.5494412450074861 1107 15 0.5427845230915338 1107 16 0.5860635940289285 1107 17 0.5933089033871326 1107 18 0.5907639599138861 1107 19 0.5082429633889518 1107 20 0.5499762350920179 1107 21 0.5632977084940194 1107 22 0.4708669032172921 1107 23 0.5390927582095983 1107 24 0.5293776072783487 1107 25 0.4976072809939822 1107 26 0.5219325033526019 1107 27 0.5927473735203677 1107 28 0.5263079157867026 1107 29 0.5496366101866457 1107 30 0.5719453509832704 1107 31 0.7381186145368304 1107 32 0.6419311975533404 1107 33 0.6814517451482232 1107 34 0.6093063769038949 1107 35 0.5398192928897245 1107 36 0.717497245871435 1107 37 0.6110695541643096 1107 38 0.5575849230516781 1107 39 0.548819922681389 1107 40 0.5483377321892107 1107 41 0.5883076108244367 1107 42 0.6893040485383608 1107 43 0.6454624157767697 1107 44 0.614380052935914 1107 45 0.6544531473014175 1107 46 0.6609276331412843 1107 47 0.7799555032356693 1107 48 0.6912412460540978 1107 49 0.732927439867936 1107 50 0.8114757982531273 1108 1 0.37285493350332644 1108 2 0.5381336269166219 1108 3 0.4072882291202511 1108 4 0.5058878676924508 1108 5 0.47506929551119015 1108 6 0.5648180017106199 1108 7 0.5596291936726274 1108 8 0.5059472418792126 1108 9 0.5480196834396551 1108 10 0.5515254672307179 1108 11 0.5649103047740667 1108 12 0.6255743856471412 1108 13 0.6696567814942611 1108 14 0.7062620459435109 1108 15 0.7701708064534429 1108 16 0.7059364001332895 1108 17 0.7279945638548853 1108 18 0.6636171876352108 1108 19 0.7028759048761515 1108 20 0.6395270337850079 1108 21 0.6583546078773921 1108 22 0.6998660627855386 1108 23 0.7869458282003947 1108 24 0.7429223299739434 1108 25 0.7163079426137031 1108 26 0.7033904860074642 1108 27 0.74046375097452 1108 28 0.6848470004318656 1108 29 0.7359101601699294 1108 30 0.6964126841212348 1108 31 0.7963661043914954 1108 32 0.6724259470848821 1108 33 0.6426109840125065 1108 34 0.739338007857107 1108 35 0.6977784731000085 1108 36 0.7781912993436038 1108 37 0.8095598228435748 1108 38 0.7573338813231416 1108 39 0.7669624358007638 1108 40 0.9128073975633555 1108 41 0.7973972620267631 1108 42 0.8023326551991161 1108 43 0.7275152491935373 1108 44 0.7939199236229868 1108 45 0.771610268878149 1108 46 0.8057349158098361 1108 47 0.7576821038034538 1108 48 0.6673727015021811 1108 49 0.7709878156320826 1108 50 0.7610113851966887 1109 1 0.21218291387577892 1109 2 0.36084840936691975 1109 3 0.28892530511443937 1109 4 0.2585045372564707 1109 5 0.2023564956523387 1109 6 0.13045479765701629 1109 7 0.16978067770444483 1109 8 0.14626895974769383 1109 9 0.13186547434400353 1109 10 0.16711189887188027 1109 11 0.1097663011083796 1109 12 0.18572426523847696 1109 13 0.21853884301769 1109 14 0.2481426965317084 1109 15 0.10980098346158229 1109 16 0.08596957585690745 1109 17 0.2160933429350962 1109 18 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45 -1.3900470932314661 2216 46 -1.2916950917657317 2216 47 -1.362830255579759 2216 48 -1.2997034270932133 2216 49 -1.2580262032935892 2216 50 -1.4020580226979789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70242.0 3 11373.0 4 19431.0 5 11658.0 6 8495.0 7 6663.0 8 5625.0 9 4895.0 10 4841.0 11 4673.0 12 4816.0 13 5134.0 14 5450.0 15 5945.0 16 6485.0 17 6805.0 18 7530.0 19 8451.0 20 9648.0 21 11875.0 22 15229.0 23 18158.0 24 21409.0 25 24199.0 26 26783.0 27 30043.0 28 35025.0 29 40586.0 30 47484.0 31 56677.0 32 69251.0 33 85718.0 34 108818.0 35 138883.0 36 181559.0 37 248026.0 38 396064.0 39 1034419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.42009169629569 18.144186492023394 13.828034419707128 26.60768739197379 2 14.721352565094447 19.695628542033774 38.171684313331 27.41133457954078 3 17.46696365045632 24.29650684312259 29.557736914911143 28.678792591509943 4 12.369308904665944 16.40962342453285 36.38312873865783 34.837938932143366 5 13.939608649520535 37.27074223319158 34.406861217007325 14.382787900280558 6 32.88593290852335 36.553520231137426 16.9324677952119 13.628079065127324 7 28.469027965838755 31.454026300099088 21.709020302006465 18.367925432055696 8 26.74252814155471 34.59832271222644 19.599168040101066 19.059981106117778 9 26.642807635128396 14.913622417957473 18.727540559698276 39.71602938721585 10 14.35745634886875 28.567282044609478 33.80924260827975 23.266018998242018 11 35.2912703962204 22.363166045360362 23.414739473183737 18.930824085235503 12 23.38815942345022 24.297061841112775 30.305112375014748 22.00966636042226 13 28.361661535095763 21.28309648747457 25.81635946949101 24.538882507938652 14 22.379138353124215 20.425932916924065 26.843383210927314 30.351545519024413 15 23.88258555437548 28.460416254821936 23.44846396958861 24.208534221213977 16 23.97212648187996 27.421710298880274 24.891758100133455 23.714405119106306 17 22.684687325716084 27.41405568505198 26.383009910051626 23.518247079180313 18 22.942753320596275 26.770448409202714 27.187167511354666 23.09963075884634 19 23.6401384182013 26.582687142187556 27.18543341135654 22.591741028254603 20 23.786702798940343 25.986171540114334 27.107892732908372 23.119232928036954 21 23.98152951384482 25.83833819966387 27.07890391192913 23.101228374562183 22 23.759030562340193 25.662705994062566 27.023010416249576 23.55525302734766 23 23.59566749833672 25.58713534295039 27.009780911724402 23.80741624698849 24 23.42195737451859 25.793682145337897 26.993290188531795 23.791070291611717 25 23.416126175820068 25.665006615300523 27.17535426087199 23.74351294800742 26 23.44954658456593 25.99429289285696 27.156377738707153 23.399782783869952 27 23.426429302511316 26.041417535475386 26.95117761521167 23.580975546801632 28 23.44402486314154 25.76059984992312 27.192836635346428 23.602538651588908 29 23.59339401841001 25.90184832925378 26.95369042031142 23.55106723202479 30 23.55722775816614 25.99322579203718 26.917056168729616 23.532490281067073 31 23.60499188359564 25.62953520403257 27.02678645795538 23.738686454416406 32 23.400564357405386 25.99150389738332 26.933017052973383 23.674914692237905 33 23.554774917941902 25.85161523001108 26.85836610116906 23.73524375087795 34 23.70321972797334 25.827592541891637 26.872479728816856 23.596708001318174 35 23.61690537940027 25.82786948527835 26.973133022420605 23.58209211290078 36 23.645080384266066 25.916620826242813 26.780350917827157 23.65794787166397 37 23.77975641592003 25.649669535977903 26.91026504315404 23.660309004948026 38 23.696334532114392 25.744127080800133 26.90298451449367 23.656553872591804 39 23.727044739504723 26.00724732728448 26.579084966853074 23.68662296635772 40 23.563335463313592 25.817469268959325 27.050713543386557 23.56848172434053 41 23.527145565560165 25.834558843237776 26.785721305646042 23.85257428555602 42 23.832081141864244 25.79865847604909 26.756394646340937 23.61286573574573 43 23.60265604971034 25.725380241011386 26.783669032277203 23.88829467700107 44 23.672835899409662 25.951195769056344 26.508617377845585 23.86735095368841 45 23.670988127715507 26.00345497701804 26.449978951370877 23.875577943895575 46 23.849656474664606 25.809319674749798 26.336596937182 24.0044269134036 47 23.64654364672947 25.98806298273914 26.412776939586628 23.95261643094476 48 23.60245916870835 26.157112921070464 26.305379181401573 23.935048728819616 49 23.795616003659287 25.83534641718721 26.38731256879029 23.981725010363213 50 23.867986727916936 25.950781798592388 26.312672981090678 23.8685584924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 456.0 1 331.0 2 206.0 3 354.5 4 503.0 5 565.5 6 628.0 7 631.5 8 635.0 9 820.5 10 1006.0 11 1443.5 12 1881.0 13 3503.5 14 5126.0 15 7026.5 16 8927.0 17 10550.0 18 12173.0 19 13209.0 20 14245.0 21 15811.5 22 17378.0 23 20139.0 24 22900.0 25 27258.0 26 31616.0 27 37430.0 28 43244.0 29 49327.5 30 55411.0 31 62310.5 32 69210.0 33 77867.5 34 86525.0 35 95530.0 36 104535.0 37 113915.0 38 123295.0 39 131246.5 40 139198.0 41 148150.5 42 157103.0 43 163201.0 44 169299.0 45 177307.0 46 185315.0 47 191064.5 48 196814.0 49 198008.5 50 199203.0 51 201379.0 52 203555.0 53 201022.0 54 198489.0 55 190027.5 56 181566.0 57 169093.5 58 156621.0 59 143224.0 60 129827.0 61 113622.0 62 97417.0 63 83305.0 64 69193.0 65 57724.5 66 46256.0 67 37631.5 68 29007.0 69 23197.5 70 17388.0 71 13480.0 72 9572.0 73 7824.5 74 6077.0 75 4497.5 76 2918.0 77 2292.0 78 1666.0 79 1302.0 80 938.0 81 713.0 82 488.0 83 380.0 84 272.0 85 220.0 86 168.0 87 117.5 88 67.0 89 44.5 90 22.0 91 16.5 92 11.0 93 9.0 94 7.0 95 6.5 96 6.0 97 4.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015509050638837094 2 3.2161625748740514E-4 3 3.2161625748740514E-4 4 0.0024657246407367725 5 0.03852248061904697 6 0.02583650601815488 7 0.028945463173866467 8 0.022691813722722474 9 0.029410019990237162 10 0.03016045792437444 11 0.03695013447133077 12 0.037021604750772416 13 0.034913231507243875 14 0.039951886207879886 15 0.035484993742777034 16 0.04141702693643362 17 0.04309657850331229 18 0.04566950856321153 19 0.04770641152729843 20 0.044168632694936975 21 0.04484760034963261 22 0.04281069738554571 23 0.04227467028973336 24 0.039987621347600706 25 0.037986453523234626 26 0.04116688095838786 27 0.040916734980342095 28 0.040487913303692225 29 0.038701156317651086 30 0.035413523463335386 31 0.03376970703617754 32 0.030303398483257728 33 0.023335046237697284 34 0.01972579712589418 35 0.020797851317518868 36 0.022048581207747664 37 0.017224337345436585 38 0.018725213213711144 39 0.013186266556983612 40 0.007790260459139369 41 0.00754011448109361 42 0.007325703642768674 43 0.0026801354790617097 44 0.0044668924651028495 45 0.0030017517365491147 46 0.0010005839121830383 47 4.288216766498735E-4 48 7.861730738581015E-4 49 2.1441083832493676E-4 50 3.9308653692905076E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2798366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.383683241970196 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9046273243107 43.732080350603816 2 13.57824198809569 15.854955584315968 3 4.9572069716543625 8.682600047938639 4 2.346950033779433 5.480943494276387 5 1.2626216557389434 3.6858251401557207 6 0.7250167024906603 2.539748730201147 7 0.47982255254601774 1.9609665544140211 8 0.3287877169868677 1.5356670337929461 9 0.24460704790058435 1.2852954363043032 >10 1.095305501226785 10.916591392348046 >50 0.05923783411107338 2.3677458154320785 >100 0.01683612809126586 1.5182351231731197 >500 4.92362045079725E-4 0.17520631949962331 >1k 2.4618102251118545E-4 0.26413897754411814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.573513972082279E-5 0.0 0.0 0.0 0.0 3 3.573513972082279E-5 0.0 0.0 0.0 0.0 4 3.573513972082279E-5 0.0 0.0 0.0 0.0 5 7.147027944164558E-5 0.0 0.0 0.0 0.0 6 7.147027944164558E-5 0.0 0.0 0.0 1.0720541916246838E-4 7 7.147027944164558E-5 0.0 0.0 0.0 1.0720541916246838E-4 8 7.147027944164558E-5 0.0 0.0 0.0 1.4294055888329116E-4 9 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 10 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 11 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 12 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 13 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 14 7.147027944164558E-5 0.0 0.0 0.0 1.7867569860411398E-4 15 7.147027944164558E-5 0.0 0.0 3.573513972082279E-5 2.1441083832493676E-4 16 7.147027944164558E-5 0.0 0.0 3.573513972082279E-5 2.8588111776658233E-4 17 7.147027944164558E-5 0.0 0.0 3.573513972082279E-5 2.8588111776658233E-4 18 7.147027944164558E-5 0.0 0.0 7.147027944164558E-5 3.9308653692905076E-4 19 1.4294055888329116E-4 0.0 0.0 7.147027944164558E-5 3.9308653692905076E-4 20 1.4294055888329116E-4 0.0 0.0 7.147027944164558E-5 3.9308653692905076E-4 21 1.4294055888329116E-4 0.0 0.0 7.147027944164558E-5 3.9308653692905076E-4 22 1.4294055888329116E-4 0.0 0.0 1.4294055888329116E-4 3.9308653692905076E-4 23 1.4294055888329116E-4 0.0 0.0 2.1441083832493676E-4 5.002919560915191E-4 24 1.7867569860411398E-4 0.0 0.0 3.5735139720822795E-4 5.360270958123419E-4 25 1.7867569860411398E-4 0.0 0.0 4.288216766498735E-4 5.360270958123419E-4 26 1.7867569860411398E-4 0.0 0.0 5.002919560915191E-4 5.360270958123419E-4 27 1.7867569860411398E-4 0.0 0.0 8.57643353299747E-4 5.360270958123419E-4 28 1.7867569860411398E-4 0.0 0.0 0.001715286706599494 5.717622355331647E-4 29 2.5014597804575957E-4 0.0 0.0 0.0030017517365491147 5.717622355331647E-4 30 2.5014597804575957E-4 0.0 0.0 0.005574681796448356 5.717622355331647E-4 31 2.5014597804575957E-4 0.0 0.0 0.011613920409267408 5.717622355331647E-4 32 2.5014597804575957E-4 0.0 0.0 0.01804624555901551 5.717622355331647E-4 33 2.5014597804575957E-4 3.573513972082279E-5 0.0 0.024371365289601144 5.717622355331647E-4 34 2.5014597804575957E-4 3.573513972082279E-5 0.0 0.0343414692717107 6.074973752539875E-4 35 2.8588111776658233E-4 3.573513972082279E-5 0.0 0.044204367834657796 6.074973752539875E-4 36 2.8588111776658233E-4 3.573513972082279E-5 0.0 0.05649725589862084 6.074973752539875E-4 37 2.8588111776658233E-4 3.573513972082279E-5 0.0 0.07432909061931141 6.074973752539875E-4 38 3.5735139720822795E-4 3.573513972082279E-5 0.0 0.09380474176715983 6.789676546956331E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1755 0.0 27.457308 1 GTATCAA 4135 0.0 25.22283 1 TATCAAC 5875 0.0 17.527548 2 ATCAACG 5960 0.0 17.130209 3 TCAACGC 6015 0.0 16.937298 4 CAACGCA 6085 0.0 16.706892 5 AACGCAG 6320 0.0 16.117893 6 TCGATCG 90 0.004370866 14.6616335 41 GTACATG 6510 0.0 14.22958 1 CGCTACG 545 0.0 14.129399 28 TATACAC 1065 0.0 14.049157 3 CTAATAC 385 0.0 13.716441 3 TACATGG 6620 0.0 13.660514 2 ACGCAGA 7470 0.0 13.547959 7 CGTGCCG 245 1.5352271E-9 13.470302 10 CGCAGAG 7705 0.0 13.163304 8 CCTATAC 335 0.0 13.136394 3 AGAGTAC 7490 0.0 12.954381 11 TATCTCG 550 0.0 12.799038 36 ACATGGG 6990 0.0 12.780265 3 >>END_MODULE