FastQCFastQC Report
Mon 6 Feb 2017
SRR2077437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421329
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111600.46090390855600377No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62490.2580814090113322No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50420.20823275151786477No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41140.16990669173829745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38100.1573516031898185No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34340.14182294103775242No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28090.11601067017328087No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26420.10911363139829407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1800.025.66783510
CCGTCGT2150.022.5126559
TACCGTC2200.022.0010017
ACCGTCG2250.021.5120938
ATACCGT2150.020.4652026
TCGTCCG750.001290982917.60080110
CGCGGGA1408.40173E-817.28221544
GTCGTAG3250.016.9238511
TCCGACC2750.016.79902819
CGAACGA1602.1314918E-816.5004116
CGTCGAG800.001989069616.5000734
GTACATG90000.015.6696031
TACATGG87950.015.6345952
GGCGTTA2001.2823875E-915.39688342
CCGACCA3150.015.36419220
ACATGGG91100.014.9004813
AGCGCGT1057.158894E-414.66763932
CGTGCGC1951.5170372E-814.667335510
CCGATAA1503.252031E-614.6673359
GCGTCGA900.004360561314.66672933
Produced by FastQC (version 0.11.5)