##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077437_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2421329
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.835947531293765	31.0	30.0	34.0	20.0	34.0
2	28.77238409154642	31.0	30.0	34.0	16.0	34.0
3	28.895150555748515	31.0	30.0	34.0	18.0	34.0
4	32.2078779876671	35.0	33.0	37.0	19.0	37.0
5	32.33314927463389	35.0	33.0	37.0	22.0	37.0
6	32.34406228975905	36.0	33.0	37.0	22.0	37.0
7	32.17721094489844	36.0	33.0	37.0	19.0	37.0
8	32.1014938490391	36.0	33.0	37.0	18.0	37.0
9	33.25580249524125	38.0	33.0	39.0	17.0	39.0
10	33.16166452390402	38.0	33.0	39.0	17.0	39.0
11	33.21572780898424	38.0	33.0	39.0	17.0	39.0
12	33.131761524352946	38.0	33.0	39.0	17.0	39.0
13	33.13586092596256	38.0	33.0	39.0	16.0	39.0
14	33.92439317416179	39.0	33.0	41.0	13.0	41.0
15	34.03914420551689	39.0	33.0	41.0	16.0	41.0
16	33.94789225256047	39.0	33.0	41.0	13.0	41.0
17	33.78410079753722	39.0	33.0	41.0	11.0	41.0
18	33.61035736985763	38.0	33.0	41.0	11.0	41.0
19	33.977083246432024	39.0	33.0	41.0	16.0	41.0
20	34.01421326882881	39.0	33.0	41.0	16.0	41.0
21	34.02145268156455	39.0	33.0	41.0	16.0	41.0
22	33.92683769946174	39.0	33.0	41.0	13.0	41.0
23	33.65753972301988	39.0	33.0	41.0	10.0	41.0
24	33.81462618256338	39.0	33.0	41.0	13.0	41.0
25	33.70500497867081	39.0	33.0	41.0	10.0	41.0
26	33.75355393670171	39.0	33.0	41.0	10.0	41.0
27	33.72942627788293	39.0	33.0	41.0	10.0	41.0
28	33.60885654118049	39.0	33.0	41.0	10.0	41.0
29	33.57814489480777	39.0	33.0	41.0	10.0	41.0
30	33.34155209804202	38.0	32.0	41.0	9.0	41.0
31	33.26332770143999	38.0	32.0	41.0	8.0	41.0
32	33.2558202540836	38.0	32.0	41.0	8.0	41.0
33	33.23176941258293	38.0	32.0	40.0	8.0	41.0
34	33.19164516676585	38.0	32.0	40.0	7.0	41.0
35	33.129603205512346	38.0	32.0	40.0	2.0	41.0
36	33.010151449885576	38.0	32.0	40.0	2.0	41.0
37	32.88760882969642	38.0	32.0	40.0	2.0	41.0
38	32.783174033764105	38.0	32.0	40.0	2.0	41.0
39	32.723448568947056	38.0	32.0	40.0	2.0	41.0
40	32.698453617827234	38.0	32.0	40.0	2.0	41.0
41	32.57692779461197	38.0	32.0	40.0	2.0	41.0
42	32.38604130211136	38.0	31.0	40.0	2.0	41.0
43	32.272250900228755	38.0	31.0	40.0	2.0	41.0
44	32.18032658924087	38.0	31.0	40.0	2.0	41.0
45	32.09122758617272	38.0	31.0	40.0	2.0	41.0
46	31.90267245797659	38.0	31.0	40.0	2.0	41.0
47	31.7086756900859	38.0	30.0	40.0	2.0	41.0
48	31.49615479763386	38.0	30.0	40.0	2.0	41.0
49	31.38896862012556	38.0	30.0	40.0	2.0	41.0
50	31.175936025215904	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9646858761198231
1101	2	0.8762199846749326
1101	3	1.0839150437765213
1101	4	1.026256558409095
1101	5	0.9706787169278641
1101	6	0.9966571401452526
1101	7	1.0520218895321705
1101	8	1.001232162640406
1101	9	1.3700178544443276
1101	10	1.3275040792137247
1101	11	1.3324291984090024
1101	12	1.3340096079522823
1101	13	1.2737646186022573
1101	14	1.6133423767424944
1101	15	1.6002337018520052
1101	16	1.4739956140695654
1101	17	1.6971498331355832
1101	18	1.8408474304220732
1101	19	1.5199524223505563
1101	20	1.5901040301631184
1101	21	1.5060662603883301
1101	22	1.4727545441802903
1101	23	1.4894709283156047
1101	24	1.4135398103091532
1101	25	1.4311839461198517
1101	26	1.4126263638914978
1101	27	1.5188610285144293
1101	28	1.5820013756879732
1101	29	1.5570308552507797
1101	30	1.7334022436597465
1101	31	1.6297776530630088
1101	32	1.691557913938631
1101	33	1.5324423568318153
1101	34	1.6211982524594006
1101	35	1.6183839814205285
1101	36	1.6655626769474239
1101	37	1.6091392953501042
1101	38	1.658162244810903
1101	39	1.6983470347586618
1101	40	1.6837123513203238
1101	41	1.6457412342780557
1101	42	1.7685266441240657
1101	43	1.8237356759922747
1101	44	1.6415643955295565
1101	45	1.6627976538263667
1101	46	1.6394801412736406
1101	47	1.6654006785166047
1101	48	1.595872728907647
1101	49	1.6895077844054747
1101	50	1.7429739384071645
1102	1	0.3201965087653136
1102	2	0.29974273578873323
1102	3	0.2573719477699612
1102	4	0.2823033701093891
1102	5	0.17574881528771158
1102	6	0.38111866496645064
1102	7	0.4421988794467637
1102	8	0.35120465462021144
1102	9	0.5894017040212489
1102	10	0.567127920840278
1102	11	0.3528284089580467
1102	12	0.4403559550365941
1102	13	0.4226642815559387
1102	14	0.8009922175077548
1102	15	0.5354901499982461
1102	16	0.6939769592799578
1102	17	0.8383548013373527
1102	18	0.9745046623033176
1102	19	0.7227690227749619
1102	20	0.6286848404123404
1102	21	0.5125371405345547
1102	22	0.7314248480546013
1102	23	0.8826979958177503
1102	24	0.6230507326494958
1102	25	0.704655237424916
1102	26	0.5717689483203401
1102	27	0.6118141037941669
1102	28	0.6629451865782912
1102	29	0.4723110245988593
1102	30	0.7319959894986283
1102	31	0.7819854233804548
1102	32	0.7286763660068019
1102	33	0.5172922416358858
1102	34	0.4277788723933966
1102	35	0.4661447084117114
1102	36	0.565389477098897
1102	37	0.5903465596758721
1102	38	0.6375064659507004
1102	39	0.6827490617132739
1102	40	0.6820219233968601
1102	41	0.7591369580962279
1102	42	0.6692669411327401
1102	43	0.7769464660213217
1102	44	0.8104272652883324
1102	45	0.6457000552408019
1102	46	0.6374453759427077
1102	47	0.634912162709071
1102	48	0.5970769761199826
1102	49	0.5463008526441975
1102	50	0.5888680497422207
1103	1	0.2324871701334672
1103	2	0.19982789048643212
1103	3	0.3408235515167668
1103	4	0.4032230408445585
1103	5	0.34918054960505174
1103	6	0.35046297379415137
1103	7	0.49641403698295505
1103	8	0.482626738071815
1103	9	0.4721720701864527
1103	10	0.6017574979052824
1103	11	0.48339894543264705
1103	12	0.566384907633811
1103	13	0.49107188870893026
1103	14	0.7936121437070156
1103	15	0.5536564855077586
1103	16	0.6707013419083978
1103	17	0.8417609892453868
1103	18	0.36281008381907043
1103	19	0.4380684834618762
1103	20	0.5333115789874157
1103	21	0.5298519290670498
1103	22	0.6715327106716913
1103	23	0.8741825260476688
1103	24	0.5850149676764644
1103	25	0.35381788289752336
1103	26	0.4528362205315233
1103	27	0.4761342854575261
1103	28	0.5610433983296375
1103	29	0.5009797728247989
1103	30	0.7393760632993676
1103	31	0.669297373423035
1103	32	0.7008591647578655
1103	33	0.6055692782524105
1103	34	0.48227787892192
1103	35	0.4843110439212168
1103	36	0.6298231983591904
1103	37	0.6326400595339479
1103	38	0.5182898891695515
1103	39	0.6279662061924114
1103	40	0.6124789202745191
1103	41	0.7233719850618883
1103	42	0.6800532028415134
1103	43	0.7468584728336936
1103	44	0.8425022014223131
1103	45	0.5843886728962104
1103	46	0.5292989098626641
1103	47	0.5537313509009536
1103	48	0.637951231016384
1103	49	0.4668231347901006
1103	50	0.6450701502247647
1104	1	0.6594130586563409
1104	2	0.5841606124385095
1104	3	0.7091201103637879
1104	4	0.6853891068201108
1104	5	0.6352501126175909
1104	6	0.7343803224088816
1104	7	0.6757301214612852
1104	8	0.7476965373220068
1104	9	0.9815025875437513
1104	10	0.9081684120690738
1104	11	0.897608573093791
1104	12	0.7933577273330741
1104	13	0.6572133938773348
1104	14	1.0327350289667763
1104	15	0.907349966716744
1104	16	0.9725195809579645
1104	17	1.1331864323808034
1104	18	1.2246159597383866
1104	19	0.9876843619855791
1104	20	0.9377566114995517
1104	21	0.8259852794097071
1104	22	0.9166776393484
1104	23	1.075010314808658
1104	24	1.0693806933021293
1104	25	1.0856410879204077
1104	26	0.8860618325527341
1104	27	0.9456182252119376
1104	28	0.9875342204532842
1104	29	1.029910739310452
1104	30	1.1706772123697604
1104	31	1.1887717157756086
1104	32	1.1544171909183163
1104	33	1.0431513277338738
1104	34	0.9977218661200098
1104	35	1.0019167598759324
1104	36	0.8949916210959898
1104	37	0.9107890572401374
1104	38	0.9135450634029283
1104	39	0.772292069580935
1104	40	0.7580562817000214
1104	41	1.0110638925658648
1104	42	0.9154741088220106
1104	43	0.9334539513225053
1104	44	1.0021111462482821
1104	45	0.869934273920947
1104	46	0.8102419496979358
1104	47	0.8178807170018558
1104	48	0.6436891881751912
1104	49	0.5767850006389033
1104	50	0.8191212273838424
1105	1	0.4550315496794006
1105	2	0.4951552134000039
1105	3	0.42786601031969695
1105	4	0.45238606415276195
1105	5	0.4011116909566539
1105	6	0.2947855137813633
1105	7	0.38835730685722325
1105	8	0.4997104171640814
1105	9	0.5073488791412828
1105	10	0.5065618979267512
1105	11	0.48918754421201527
1105	12	0.501111761621921
1105	13	0.5112503562045987
1105	14	0.7110082298654987
1105	15	0.607504827651745
1105	16	0.6805995325324403
1105	17	0.5481713952155687
1105	18	0.8137956758457321
1105	19	0.7018562160531445
1105	20	0.6872591974345497
1105	21	0.6161570574453563
1105	22	0.6375467922895339
1105	23	0.916519319567918
1105	24	0.729913423572583
1105	25	0.7249569737841455
1105	26	0.7116190483444385
1105	27	0.5472201959838472
1105	28	0.7370634639187514
1105	29	0.5486346023745057
1105	30	0.9574427761564408
1105	31	0.9181866548411293
1105	32	0.6946239328163983
1105	33	0.6904698815141685
1105	34	0.7497616308105108
1105	35	0.7825724176779261
1105	36	0.7684147122661358
1105	37	0.742818257821753
1105	38	0.8625157504733423
1105	39	0.8736753713347483
1105	40	0.802484649206761
1105	41	0.9567275004934004
1105	42	1.0380248806000125
1105	43	0.8085837425886311
1105	44	0.8985889833319831
1105	45	0.799628939339577
1105	46	0.6505878361782713
1105	47	0.776447960506573
1105	48	0.9094106792599099
1105	49	0.9259451802534819
1105	50	0.78688578251138
1106	1	0.2797166439439316
1106	2	0.24902465376895933
1106	3	0.21874455884355015
1106	4	0.15447080341691333
1106	5	0.31227280083828646
1106	6	0.18420763181671163
1106	7	0.17727051208806444
1106	8	0.3272633826193925
1106	9	-1.106167455156509
1106	10	-0.7342468435704959
1106	11	0.043865616791286755
1106	12	0.23112887413372363
1106	13	0.40027858592937093
1106	14	0.6404084176568716
1106	15	0.526418479137547
1106	16	0.6039607067112129
1106	17	0.5712450536957334
1106	18	-1.3407783920050633
1106	19	-0.05792655795879398
1106	20	0.1607662241287926
1106	21	0.27623099005356977
1106	22	0.4209359985658807
1106	23	-0.9355776689396507
1106	24	0.18606614112870545
1106	25	0.3940903375356726
1106	26	0.4561116701105732
1106	27	0.5766347732210235
1106	28	0.6766929083677482
1106	29	0.579198969188802
1106	30	-0.14594630261849062
1106	31	0.5581262920267349
1106	32	0.5368354631339614
1106	33	0.6939288128519223
1106	34	0.633505382102598
1106	35	0.5598529399237364
1106	36	0.787754400634455
1106	37	0.7528207440994095
1106	38	0.7006959591567394
1106	39	0.6877309455601548
1106	40	0.6976803046870046
1106	41	0.8622357439154626
1106	42	0.912394090100328
1106	43	0.8446821263260063
1106	44	0.8060398019366275
1106	45	0.8923775693823686
1106	46	0.8955098645072646
1106	47	0.8466739203418072
1106	48	0.9295235726587059
1106	49	0.8722010555643784
1106	50	1.0274559142112416
1107	1	0.4008398225352856
1107	2	0.5064065370193944
1107	3	0.42307996950111004
1107	4	0.4976375302298095
1107	5	0.5123667995388956
1107	6	0.4627396638739114
1107	7	0.5633075286351854
1107	8	0.40749918524211637
1107	9	0.8061830211751442
1107	10	0.7442356597596032
1107	11	0.752084209063689
1107	12	0.6722561585506739
1107	13	0.6058403031644559
1107	14	0.940271625430178
1107	15	0.8509582575582542
1107	16	0.6892393808001316
1107	17	0.9659132744585648
1107	18	0.8555609527157628
1107	19	0.7342057272713802
1107	20	0.8178486115193166
1107	21	0.6547428165077349
1107	22	0.8168325847013094
1107	23	0.9582014464666955
1107	24	0.8794002332750779
1107	25	0.8141649717980854
1107	26	0.7505968639917171
1107	27	0.6318732743358595
1107	28	0.6254696458657918
1107	29	0.7290435154553663
1107	30	0.8773844042805621
1107	31	1.0116364935033602
1107	32	0.8651368072036121
1107	33	0.8429535175104164
1107	34	0.656661826704692
1107	35	0.6066658222162431
1107	36	0.7091801926896792
1107	37	0.6356608930738261
1107	38	0.8045183474943087
1107	39	0.8752921763313779
1107	40	0.8054020508863928
1107	41	0.7513738940645638
1107	42	0.7965643677464911
1107	43	0.8556731508618469
1107	44	0.6086000312511004
1107	45	0.7293979105717696
1107	46	0.8692741977650051
1107	47	0.9024009441152998
1107	48	0.7496015009932258
1107	49	0.8075041557862299
1107	50	1.0072703108945795
1108	1	0.17035110686330412
1108	2	0.29156217577352095
1108	3	0.3281939123240214
1108	4	0.2968569849404261
1108	5	0.23236810280765496
1108	6	0.22600118208954711
1108	7	0.14985143759478348
1108	8	0.27323418331105387
1108	9	0.27037253321368837
1108	10	0.21261297022611103
1108	11	0.28826371569699205
1108	12	0.22229871706664994
1108	13	0.2808542714025535
1108	14	0.14456001540055752
1108	15	0.28410519359795927
1108	16	0.5835902308651271
1108	17	0.289231714599012
1108	18	0.6481432728491399
1108	19	0.4974115459185029
1108	20	0.3942648796077677
1108	21	0.4458819326458823
1108	22	0.45018667112073985
1108	23	0.2572160192365516
1108	24	0.3564840148177595
1108	25	0.4172213826739366
1108	26	0.3966625889046185
1108	27	0.3665563625742365
1108	28	0.3869350516656809
1108	29	0.4134004903055981
1108	30	0.5073578664342548
1108	31	0.6553603802669343
1108	32	0.6031165007736305
1108	33	0.5252384106170922
1108	34	0.5385408859642595
1108	35	0.3878800843689021
1108	36	0.4871697315738359
1108	37	0.39736275112763053
1108	38	0.35869127103101306
1108	39	0.6020923668802496
1108	40	0.5060456229177817
1108	41	0.3908336934969938
1108	42	0.37218054151411906
1108	43	0.5990241322651215
1108	44	0.4178440356729709
1108	45	0.28107049420384556
1108	46	0.48196497239935
1108	47	0.4799997624464254
1108	48	0.5067915495103641
1108	49	0.41730678859141435
1108	50	0.4941709427198546
1109	1	-0.11312479279759557
1109	2	-0.03764992790539523
1109	3	-0.055563515703287436
1109	4	-0.154364779425741
1109	5	-0.06567136701818299
1109	6	-0.11256405082209398
1109	7	-0.11044074609934285
1109	8	-0.07089043819387086
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2211	9	-0.15327609394364572
2211	10	-0.2836300033020791
2211	11	-0.10903997711267266
2211	12	-0.1437556456897866
2211	13	-0.24223386339696873
2211	14	-0.11868517383865651
2211	15	-0.43671942137793707
2211	16	-0.36074621251583494
2211	17	-0.11371746640901392
2211	18	0.12617210133309698
2211	19	-0.06369621711293405
2211	20	-0.14491704059384602
2211	21	-0.14225034472756448
2211	22	-0.21352057646678446
2211	23	0.05937543020743874
2211	24	-0.05127995542027719
2211	25	-0.15829880078183578
2211	26	-0.14399563125996195
2211	27	-0.19531302046942045
2211	28	-0.2758871305539543
2211	29	-0.17206264906532454
2211	30	-0.07198779191131877
2211	31	-0.12359536730539133
2211	32	-0.15365698512564308
2211	33	-0.17844929869529835
2211	34	-0.32294158486529057
2211	35	-0.3566794574176342
2211	36	-0.19092394425474168
2211	37	-0.220099778442858
2211	38	-0.18066628907223503
2211	39	-0.23991621376298156
2211	40	-0.3124926467538529
2211	41	-0.12265036460723877
2211	42	-0.0876739358881693
2211	43	-0.1706936010315374
2211	44	-0.12816395030274208
2211	45	-0.21126209196514978
2211	46	-0.43463851201045145
2211	47	-0.31016120833569616
2211	48	-0.22102692444441274
2211	49	-0.17819108502380132
2211	50	-0.025375179113282798
2212	1	-0.30172834784588787
2212	2	-0.34338549036532484
2212	3	-0.5683563351538936
2212	4	-0.368079826030268
2212	5	-0.30559802565322514
2212	6	-0.36601130633326306
2212	7	-0.329169918490301
2212	8	-0.417436489666688
2212	9	-0.5817011168970652
2212	10	-0.35205381310160533
2212	11	-0.3370685193152596
2212	12	-0.3662165007978544
2212	13	-0.4504968200184507
2212	14	-0.6785479771615925
2212	15	-0.5719394847376549
2212	16	-0.6222480143250948
2212	17	-0.25414264927817953
2212	18	-0.15692508056554288
2212	19	-0.4958667148644622
2212	20	-0.6242273901751716
2212	21	-0.40992117196066147
2212	22	-0.2954022608758322
2212	23	-0.24136128506809484
2212	24	-0.27852973553613936
2212	25	-0.3464588323238402
2212	26	-0.36559969642838297
2212	27	-0.3593623076036465
2212	28	-0.4050075112637046
2212	29	-0.4253804871147935
2212	30	-0.16516724770150404
2212	31	-0.3646888876849985
2212	32	-0.3897746445106307
2212	33	-0.3139438381241746
2212	34	-0.4735918629256801
2212	35	-0.24469914911111346
2212	36	-0.27925891810300385
2212	37	-1.5194197229686424
2212	38	-0.888886233873734
2212	39	-0.5921906016027734
2212	40	-0.5027302043441964
2212	41	-1.3592749293193478
2212	42	-0.8811746017442275
2212	43	-0.7567865837633931
2212	44	-0.5805137466858881
2212	45	-0.47103239230345295
2212	46	-0.3866010641553892
2212	47	-0.3717807412163019
2212	48	-0.5350905488519686
2212	49	-0.3979321318101867
2212	50	-0.2710753756993576
2213	1	-0.5384075337205374
2213	2	-0.6399553390322446
2213	3	-0.5647151577063347
2213	4	-0.6935612293539286
2213	5	-0.7104639780132445
2213	6	-0.6396345074821852
2213	7	-0.6134812713323825
2213	8	-0.6435096593431275
2213	9	-0.7138323550425483
2213	10	-0.6726965495185411
2213	11	-0.7523173891671391
2213	12	-0.506849704752085
2213	13	-0.5597280959867348
2213	14	-2.6427449962781573
2213	15	-1.3759184156224222
2213	16	-1.012709619993764
2213	17	-0.7166742787710305
2213	18	-0.8147407927996113
2213	19	-0.7277663406838997
2213	20	-0.9526389441340726
2213	21	-0.9043884356972498
2213	22	-0.7851744029165033
2213	23	-1.5139192369982482
2213	24	-1.0259850006516515
2213	25	-0.8992711181749016
2213	26	-0.9142735954847652
2213	27	-0.7913890096646412
2213	28	-0.6780034261219257
2213	29	-1.1709413322799662
2213	30	-0.9451004367220079
2213	31	-0.8499330401541556
2213	32	-0.9572237965680657
2213	33	-0.9498723600062746
2213	34	-0.9453664222760736
2213	35	-0.9345434475679184
2213	36	-0.8690026581500945
2213	37	-1.0379586881311873
2213	38	-0.9803113440274096
2213	39	-1.0427526400703755
2213	40	-1.1813954222998397
2213	41	-1.3550552444349293
2213	42	-1.1149314527656529
2213	43	-1.1445940335993292
2213	44	-1.1245322310103596
2213	45	-1.1435690601843582
2213	46	-1.2498845439648534
2213	47	-1.3358132799916547
2213	48	-1.376160878329049
2213	49	-1.4596253093303204
2213	50	-1.4095113679338205
2214	1	-0.7606476074871722
2214	2	-0.6786575855652188
2214	3	-0.9018749491779232
2214	4	-0.986233513498032
2214	5	-0.8059962836327124
2214	6	-0.7973476194385292
2214	7	-0.9912446802040513
2214	8	-1.0302934415128107
2214	9	-1.1145702934485584
2214	10	-1.2561643702807537
2214	11	-1.0918256488016311
2214	12	-1.155394771384067
2214	13	-1.2101992248472087
2214	14	-1.6270112934949452
2214	15	-1.4005682166154898
2214	16	-1.1619009472993227
2214	17	-1.6663472984864356
2214	18	-1.7246117460861434
2214	19	-1.2799373507401555
2214	20	-1.2767850277508543
2214	21	-1.4367505800175024
2214	22	-1.2659626872124292
2214	23	-0.9986913330118625
2214	24	-1.0871491078739197
2214	25	-1.3059673609659441
2214	26	-1.3959628083799416
2214	27	-1.330708744293041
2214	28	-1.3867201160810012
2214	29	-1.1269200359467604
2214	30	-1.086078179262259
2214	31	-1.0673926486920067
2214	32	-1.1001589586855616
2214	33	-1.163418557815504
2214	34	-1.3182808014774352
2214	35	-1.217074180150405
2214	36	-1.404239339473314
2214	37	-1.3566028834369988
2214	38	-1.404711496991414
2214	39	-1.2571047421486803
2214	40	-1.302916440089998
2214	41	-1.1895368054295972
2214	42	-1.1493512105345545
2214	43	-1.3331250934954042
2214	44	-1.300909903544138
2214	45	-1.3180125080561318
2214	46	-1.3612682274507115
2214	47	-1.3099972713100918
2214	48	-1.3616592268646883
2214	49	-1.3862842797291215
2214	50	-1.314219990143613
2215	1	-0.3962078062959371
2215	2	-0.5314846189056297
2215	3	-0.43537703232165015
2215	4	-0.24749159073614635
2215	5	-0.4666507800883366
2215	6	-0.3604557507777102
2215	7	-0.4494974939649339
2215	8	-0.48051616598165836
2215	9	-0.37623893202312075
2215	10	-0.7617682544336901
2215	11	-0.5957481022595488
2215	12	-0.6947179013580822
2215	13	-0.6933384010953247
2215	14	-0.6749220823592239
2215	15	-0.6539189431877048
2215	16	-0.5314722595787487
2215	17	-0.6990228235701181
2215	18	-0.6043807294917798
2215	19	-0.7427349065855964
2215	20	-0.7996164346818588
2215	21	-0.7158618815778439
2215	22	-1.025969487766588
2215	23	-0.8831874599825085
2215	24	-0.6552648740359786
2215	25	-0.7071186518071855
2215	26	-0.7222134530421371
2215	27	-0.6768304059540995
2215	28	-0.7333999793620549
2215	29	-0.6962216013382587
2215	30	-0.5527567279380463
2215	31	-0.6958202291104527
2215	32	-0.8746213609749915
2215	33	-0.814111127262052
2215	34	-0.8002453020791194
2215	35	-0.9290556694970462
2215	36	-0.8546946479504953
2215	37	-0.7180463958600214
2215	38	-0.6114080203661061
2215	39	-0.7894322760503272
2215	40	-0.8017264695169324
2215	41	-0.9087613905705147
2215	42	-0.8229229677572967
2215	43	-0.8770052267761521
2215	44	-0.8736782347033163
2215	45	-0.8053227751232477
2215	46	-0.7015364827672812
2215	47	-0.6567675137030768
2215	48	-0.792093349972415
2215	49	-0.8332184685671109
2215	50	-0.9703011771310415
2216	1	-0.9073413386562486
2216	2	-1.0964835132001909
2216	3	-0.9465483429511679
2216	4	-2.2953354651280122
2216	5	-1.3522720022614152
2216	6	-1.108309760802772
2216	7	-1.0221036416871812
2216	8	-1.0859184584776038
2216	9	-1.0538032472228807
2216	10	-1.4073970322606115
2216	11	-1.212714230816239
2216	12	-1.297497829118658
2216	13	-1.4219079868243512
2216	14	-1.291222023388272
2216	15	-1.2034296685304753
2216	16	-1.3744740655643
2216	17	-1.2413206637588203
2216	18	-1.0192579961879318
2216	19	-1.3608399117455576
2216	20	-1.3382120044887316
2216	21	-1.4091327425538083
2216	22	-1.6366459952116656
2216	23	-3.487349519543173
2216	24	-2.2530893985826417
2216	25	-1.7512694700292144
2216	26	-1.8412454079293568
2216	27	-1.8241840842675288
2216	28	-2.115928911987737
2216	29	-1.7521592395514425
2216	30	-1.4894136275399923
2216	31	-1.5261045796927952
2216	32	-1.720688735293141
2216	33	-1.7229106997226182
2216	34	-1.810928259472604
2216	35	-1.9804534654575363
2216	36	-1.8415883528008585
2216	37	-1.7144141534893862
2216	38	-1.9350258885660203
2216	39	-2.1058271234629764
2216	40	-2.195874523475357
2216	41	-2.0533434339141685
2216	42	-2.2822763143590983
2216	43	-2.243238825478791
2216	44	-2.369775094499868
2216	45	-2.4175288417604293
2216	46	-2.354002205898638
2216	47	-2.3375005964098428
2216	48	-2.4872835313081083
2216	49	-2.7804578906517357
2216	50	-2.661539382939683
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	137814.0
3	6812.0
4	13631.0
5	10236.0
6	8402.0
7	6994.0
8	6093.0
9	5524.0
10	5317.0
11	5357.0
12	5423.0
13	5663.0
14	5854.0
15	6232.0
16	6751.0
17	7277.0
18	8020.0
19	9128.0
20	11935.0
21	16359.0
22	20767.0
23	23757.0
24	25512.0
25	26364.0
26	27609.0
27	31311.0
28	36079.0
29	40134.0
30	45771.0
31	54303.0
32	66539.0
33	82237.0
34	104648.0
35	132707.0
36	169646.0
37	226587.0
38	357416.0
39	661120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.915523912092965	18.309750890385654	13.052694134654248	23.722031062867135
2	15.274546651584329	21.263246469364653	39.490338720296315	23.971868158754706
3	19.243944643113224	26.23546472583364	29.492994428687318	25.027596202365814
4	11.932684053092569	16.76397052654029	39.69088165356905	31.612463766798086
5	12.569028573696063	39.685147034995424	34.731582184510486	13.014242206798032
6	30.11419685102173	38.32944384360656	18.266619664934957	13.289739640436752
7	27.10711524691044	33.40589024981313	22.240678233627488	17.246316269648936
8	25.863222496205356	34.3030600568546	20.632045612612647	19.2016718343274
9	27.018162553865327	16.43807822063833	19.85764842201656	36.68611080347978
10	14.902099676572277	29.18883533784683	34.114929665008894	21.794135320572003
11	33.92113246204128	23.729147649251157	24.257468498068746	18.092251390638825
12	23.979709076361257	26.070257777718997	30.04574302241193	19.904290123507813
13	27.777024686959894	22.80152814984597	25.98689566872674	23.4345514944674
14	21.900145723795962	22.033264370077276	28.910634034896017	27.155955871230752
15	23.889199350565608	29.72774348002923	24.341802874838386	22.041254294566773
16	22.36963271325314	28.923211345285253	26.644656354774927	22.06249958668668
17	22.090392654434254	28.22336796322422	27.07881651834635	22.60742286399518
18	21.477653146413843	28.87567263739988	28.072599376751338	21.574074839434942
19	22.47776433689349	28.53083236452891	28.19322141351907	20.798181885058536
20	23.08434400257259	27.066853383175836	27.51660153908853	22.332201075163038
21	23.967242752564406	27.05684489328433	27.992335204203734	20.98357714994753
22	22.808764322743915	26.547608567256503	27.53004660897685	23.113580501022735
23	22.585676734612907	27.499338722742976	27.969382862999886	21.945601679644234
24	23.305147959780363	26.82079152397313	27.774294955849893	22.09976556039661
25	22.395918365659913	27.26914640391531	27.849739380009204	22.485195850415572
26	22.92897372037988	26.941581324211768	28.30650945053582	21.82293550487253
27	21.953898132464335	27.11985675420301	28.58277760279878	22.343467510533873
28	21.755460259201858	28.187840109934857	27.918832090957537	22.137867539905752
29	22.384207251915498	28.296690690901727	27.57224322419427	21.746858832988504
30	23.257765229248324	27.652478479230652	27.51157618375081	21.57818010777021
31	23.055882414937688	26.62803870731489	27.67821826987577	22.637860607871655
32	22.34113347425226	28.085425968376736	27.747981075971396	21.825459481399612
33	23.090925323387033	26.58891159431791	27.630082268603022	22.690080813692035
34	22.437549721559268	27.218836851295087	28.358181198276668	21.985432228868984
35	23.625941198451095	27.413593269997055	27.565458140849913	21.395007390701934
36	23.03953463569419	27.158928094567635	27.532291014735264	22.269246255002905
37	22.572853061916316	27.17955608104477	28.80801683402855	21.439574023010362
38	23.14039099889931	27.322349035481214	27.63780005976706	21.899459905852417
39	22.09540464907162	28.09303102080892	27.840833017896433	21.97073131222303
40	23.306888900735643	26.941913578486737	27.912038684332657	21.839158836444966
41	22.365785041183113	28.172785591613618	27.444167668075526	22.01726169912774
42	22.686285286861366	28.22081072480897	27.224782110992184	21.868121877337476
43	22.408726794352475	26.9013632930576	27.07914999528987	23.610759917300054
44	22.71724216005345	27.3266155138413	27.468951535101223	22.487190791004032
45	22.70052490488578	26.992894942997577	27.176429853469564	23.13015029864708
46	22.58422774244488	27.51441652381324	27.310806494862444	22.590549238879433
47	23.289735749811925	27.083919461179644	27.34906614083511	22.277278648173326
48	22.071619612073864	27.75221747927756	27.618055179668815	22.55810772897976
49	23.31417402874309	27.299405799856203	27.21217655760601	22.1742436137947
50	22.55909061235418	26.92910613957309	28.22958302930339	22.282220218769343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2328.0
1	1962.0
2	1596.0
3	1538.5
4	1481.0
5	1482.5
6	1484.0
7	1410.0
8	1336.0
9	1504.5
10	1673.0
11	2110.5
12	2548.0
13	4299.0
14	6050.0
15	8431.0
16	10812.0
17	11515.0
18	12218.0
19	12728.0
20	13238.0
21	15403.5
22	17569.0
23	21800.0
24	26031.0
25	32876.5
26	39722.0
27	46637.0
28	53552.0
29	60496.0
30	67440.0
31	74490.0
32	81540.0
33	89490.5
34	97441.0
35	105153.0
36	112865.0
37	119047.5
38	125230.0
39	132262.5
40	139295.0
41	144244.0
42	149193.0
43	149424.5
44	149656.0
45	160303.5
46	170951.0
47	185731.5
48	200512.0
49	191615.0
50	182718.0
51	166651.0
52	150584.0
53	141853.0
54	133122.0
55	125877.5
56	118633.0
57	109460.0
58	100287.0
59	89979.0
60	79671.0
61	69062.0
62	58453.0
63	48558.0
64	38663.0
65	31896.5
66	25130.0
67	21363.0
68	17596.0
69	15095.5
70	12595.0
71	9779.0
72	6963.0
73	5722.0
74	4481.0
75	3538.0
76	2595.0
77	2124.0
78	1653.0
79	1323.0
80	993.0
81	765.0
82	537.0
83	446.0
84	355.0
85	280.5
86	206.0
87	171.0
88	136.0
89	105.5
90	75.0
91	58.5
92	42.0
93	32.5
94	23.0
95	18.0
96	13.0
97	15.5
98	18.0
99	22.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06570771671259874
2	0.07111796868579198
3	0.06657500901364498
4	0.07979089169625442
5	0.08429255173501825
6	0.06442742807772095
7	0.04790757472445917
8	0.06079306034000336
9	0.058025984903332016
10	0.06702930498085968
11	0.05773688746964994
12	0.07124186758594143
13	0.0690116873832511
14	0.06950728298384895
15	0.08231016933262683
16	0.07669341919251782
17	0.08788561983935268
18	0.07223305878713715
19	0.06938338408369948
20	0.08156677593173005
21	0.08078208289745012
22	0.07590872615823789
23	0.07305905145480024
24	0.06261024420886216
25	0.0787171012282924
26	0.0889594103073147
27	0.08222757006586053
28	0.10085370472166318
29	0.0926763773117986
30	0.07937789536242287
31	0.07590872615823789
32	0.08140157739819744
33	0.07999738986317019
34	0.06624461194657975
35	0.08561414000327919
36	0.0923046806113502
37	0.09990381315385062
38	0.07995609022978703
39	0.058025984903332016
40	0.06351883614329155
41	0.058025984903332016
42	0.06273414310901163
43	0.041836528617135466
44	0.04129963338315446
45	0.04464490368718997
46	0.04196042751728493
47	0.03229631330562679
48	0.046503387189431915
49	0.052409234763223005
50	0.04390151028629319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2421329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.21033412676529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19140238370204	38.735579077863754
2	12.514761354740841	13.067997436954984
3	5.21436376229965	8.16731022864485
4	2.690830684163103	5.619566763948323
5	1.5433666735696274	4.028984485359228
6	0.9836176212801482	3.0813002796006366
7	0.6318395541790278	2.309198796673542
8	0.44129048819653477	1.8431939068563565
9	0.3173163410897205	1.4910472972959241
>10	1.3419253067793668	12.013421831745202
>50	0.08492426957365218	3.0536062325443245
>100	0.04164294815412167	3.6969819247724267
>500	9.595102137231885E-4	0.33593776604034903
>1k	0.0015192245050600827	1.6186130315527985
>5k	1.5991836895369292E-4	0.4713648957820759
>10k+	7.995918447684646E-5	0.4658960443652437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11160	0.46090390855600377	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	6249	0.2580814090113322	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5042	0.20823275151786477	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	4114	0.16990669173829745	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3810	0.1573516031898185	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	3434	0.14182294103775242	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	2809	0.11601067017328087	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	2642	0.10911363139829407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1299633383154456E-5
2	0.0	0.0	0.0	0.0	4.1299633383154456E-5
3	0.0	0.0	0.0	0.0	4.1299633383154456E-5
4	0.0	0.0	0.0	0.0	4.1299633383154456E-5
5	0.0	0.0	0.0	0.0	4.1299633383154456E-5
6	0.0	0.0	0.0	0.0	4.1299633383154456E-5
7	0.0	0.0	0.0	0.0	4.1299633383154456E-5
8	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
9	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
10	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
11	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
12	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
13	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
14	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
15	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
16	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
17	0.0	0.0	0.0	4.1299633383154456E-5	4.1299633383154456E-5
18	0.0	0.0	0.0	8.259926676630891E-5	4.1299633383154456E-5
19	0.0	0.0	0.0	8.259926676630891E-5	4.1299633383154456E-5
20	0.0	0.0	0.0	8.259926676630891E-5	4.1299633383154456E-5
21	0.0	0.0	0.0	2.064981669157723E-4	4.1299633383154456E-5
22	0.0	0.0	0.0	2.064981669157723E-4	4.1299633383154456E-5
23	0.0	0.0	0.0	3.716967004483901E-4	4.1299633383154456E-5
24	0.0	0.0	0.0	6.194945007473168E-4	8.259926676630891E-5
25	0.0	0.0	0.0	6.194945007473168E-4	8.259926676630891E-5
26	0.0	0.0	0.0	9.498915678125525E-4	8.259926676630891E-5
27	0.0	0.0	0.0	0.0011150901013451703	8.259926676630891E-5
28	0.0	0.0	0.0	0.001899783135625105	8.259926676630891E-5
29	0.0	0.0	0.0	0.0027257758032881944	8.259926676630891E-5
30	0.0	0.0	0.0	0.004708158205679608	8.259926676630891E-5
31	0.0	0.0	0.0	0.009664114211658144	8.259926676630891E-5
32	0.0	0.0	0.0	0.014743969117786141	8.259926676630891E-5
33	0.0	0.0	0.0	0.020154221090979377	1.2389890014946338E-4
34	0.0	0.0	0.0	0.028083750700545033	1.2389890014946338E-4
35	0.0	0.0	0.0	0.035063388742298134	1.2389890014946338E-4
36	0.0	0.0	0.0	0.04299291835186379	1.2389890014946338E-4
37	0.0	0.0	0.0	0.05662179736830476	1.2389890014946338E-4
38	0.0	0.0	0.0	0.0722743584205203	1.2389890014946338E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	180	0.0	25.667835	10
CCGTCGT	215	0.0	22.512655	9
TACCGTC	220	0.0	22.001001	7
ACCGTCG	225	0.0	21.512093	8
ATACCGT	215	0.0	20.465202	6
TCGTCCG	75	0.0012909829	17.600801	10
CGCGGGA	140	8.40173E-8	17.282215	44
GTCGTAG	325	0.0	16.92385	11
TCCGACC	275	0.0	16.799028	19
CGAACGA	160	2.1314918E-8	16.50041	16
CGTCGAG	80	0.0019890696	16.50007	34
GTACATG	9000	0.0	15.669603	1
TACATGG	8795	0.0	15.634595	2
GGCGTTA	200	1.2823875E-9	15.396883	42
CCGACCA	315	0.0	15.364192	20
ACATGGG	9110	0.0	14.900481	3
AGCGCGT	105	7.158894E-4	14.667639	32
CGTGCGC	195	1.5170372E-8	14.6673355	10
CCGATAA	150	3.252031E-6	14.667335	9
GCGTCGA	90	0.0043605613	14.666729	33
>>END_MODULE