FastQCFastQC Report
Mon 6 Feb 2017
SRR2077437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421329
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114400.472467805903287No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62010.2560990266089408No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52080.21508849065946845No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42870.17705152831358317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.17387145654308025No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32710.1350911007962982No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31700.13091983782459962No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29670.12253601224781928No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29300.12100792581264255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.11332619400337583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.10229919189007358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC1551.8189894E-1221.28896330
CTAGCGG1759.094947E-1218.85671829
CGCAATA1601.1041266E-917.8731236
CCGGTCA1408.376628E-817.28675321
TCGCGTA1802.8558134E-1017.1114319
CGCTTCG902.2179584E-417.10966532
CGTACAC1206.4044325E-616.5020143
ACCGTGC2301.8189894E-1216.2611758
GCGTAAC2051.05501385E-1016.09919411
CGCGTAA1958.931238E-1015.79582110
TCGTTTA1857.592462E-915.45719538
GTACATG87850.015.2550641
TACATGG85350.015.2356032
CGATACT1452.2838722E-615.1748934
AACCGTG1452.28731E-615.1726997
TCGTATG4400.014.99625540
ATGCCGT4600.014.82146744
ATACGAT1351.9526515E-514.6705781
TACGACG1655.413367E-714.6690635
CCGACAT1503.248757E-614.66875914
Produced by FastQC (version 0.11.5)