##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2421329
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.495126849758954	31.0	31.0	34.0	27.0	34.0
2	30.395353543446596	31.0	30.0	34.0	26.0	34.0
3	30.587126326079602	31.0	31.0	34.0	27.0	34.0
4	34.03034862259528	37.0	35.0	37.0	30.0	37.0
5	33.69365542642078	37.0	35.0	37.0	28.0	37.0
6	33.64635082634371	37.0	35.0	37.0	28.0	37.0
7	33.45868859622133	37.0	35.0	37.0	27.0	37.0
8	33.419965646964954	37.0	35.0	37.0	27.0	37.0
9	34.82826290851016	39.0	35.0	39.0	27.0	39.0
10	34.69585380590576	39.0	35.0	39.0	27.0	39.0
11	34.657218824868494	39.0	35.0	39.0	27.0	39.0
12	34.572868453646734	39.0	35.0	39.0	27.0	39.0
13	34.556195791649955	39.0	35.0	39.0	27.0	39.0
14	35.6566183281991	40.0	36.0	41.0	27.0	41.0
15	35.67849102703515	40.0	36.0	41.0	27.0	41.0
16	35.62953402862643	40.0	36.0	41.0	27.0	41.0
17	35.62289717754175	40.0	36.0	41.0	27.0	41.0
18	35.640983112992906	40.0	36.0	41.0	27.0	41.0
19	35.69141492130974	40.0	36.0	41.0	27.0	41.0
20	35.697606149350214	40.0	36.0	41.0	27.0	41.0
21	35.63270377548859	40.0	36.0	41.0	26.0	41.0
22	35.6328260224034	40.0	35.0	41.0	27.0	41.0
23	35.56653432887476	40.0	35.0	41.0	26.0	41.0
24	35.55365627719323	40.0	35.0	41.0	26.0	41.0
25	35.52355669138725	40.0	35.0	41.0	26.0	41.0
26	35.4438756567158	40.0	35.0	41.0	26.0	41.0
27	35.38010324082353	40.0	35.0	41.0	25.0	41.0
28	35.31518765107922	39.0	35.0	41.0	25.0	41.0
29	35.24347496767271	39.0	35.0	41.0	25.0	41.0
30	35.1098388529605	39.0	35.0	41.0	24.0	41.0
31	35.01304820617107	39.0	35.0	41.0	24.0	41.0
32	34.90766062769661	39.0	34.0	41.0	24.0	41.0
33	34.807281455762514	39.0	34.0	41.0	24.0	41.0
34	34.75350189916364	39.0	34.0	41.0	24.0	41.0
35	34.69051706727999	39.0	34.0	41.0	23.0	41.0
36	34.5860459276703	39.0	34.0	41.0	22.0	41.0
37	34.466336875327556	39.0	34.0	41.0	21.0	41.0
38	34.38976405106452	39.0	34.0	41.0	21.0	41.0
39	34.299848554244385	39.0	34.0	41.0	19.0	41.0
40	34.21141406227737	39.0	34.0	41.0	18.0	41.0
41	34.151300793902855	39.0	34.0	41.0	18.0	41.0
42	34.025486416757076	39.0	33.0	41.0	16.0	41.0
43	34.10366786174039	39.0	34.0	41.0	16.0	41.0
44	34.02290395068163	39.0	34.0	41.0	15.0	41.0
45	33.93956294249976	39.0	33.0	41.0	12.0	41.0
46	33.756787697995605	39.0	33.0	41.0	8.0	41.0
47	33.593960176415514	39.0	33.0	41.0	2.0	41.0
48	33.41654851529883	39.0	33.0	41.0	2.0	41.0
49	33.27580679866305	39.0	33.0	41.0	2.0	41.0
50	33.12073906519932	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8227513129852859
1101	2	0.9406182537622243
1101	3	0.8129430181823913
1101	4	0.8753687720283452
1101	5	0.9056694193402564
1101	6	0.9168898268474024
1101	7	0.8263869272040836
1101	8	0.8334861032295962
1101	9	0.9348930257573969
1101	10	0.9268934333096084
1101	11	1.0763420228787837
1101	12	1.0113432894888277
1101	13	0.9570518120659983
1101	14	1.0690378836749304
1101	15	1.0435879737028841
1101	16	1.1294964009490442
1101	17	1.1568219676321263
1101	18	1.2151605622944786
1101	19	1.135897833388519
1101	20	1.1296877528726057
1101	21	1.0542749643521105
1101	22	1.1696478568304585
1101	23	1.2506880622348078
1101	24	1.2094859773824638
1101	25	1.1659962652911346
1101	26	1.2310427294239474
1101	27	1.1701879590440711
1101	28	1.2629532724825978
1101	29	1.3574908206245908
1101	30	1.5273014107843679
1101	31	1.4333946858441493
1101	32	1.4001458718987791
1101	33	1.3288246026877104
1101	34	1.3421544674867434
1101	35	1.4540034366797272
1101	36	1.3392690870099386
1101	37	1.437059124127579
1101	38	1.4614149189605925
1101	39	1.4296248975094699
1101	40	1.5330304431048845
1101	41	1.486935545628107
1101	42	1.5721752718365423
1101	43	1.5263441045933206
1101	44	1.5661797457908762
1101	45	1.513467524465952
1101	46	1.5704220699711016
1101	47	1.5314174140986054
1101	48	1.6186639760264754
1101	49	1.7316267227616606
1101	50	1.7821574267050764
1102	1	0.3170459969548354
1102	2	0.47256401262308856
1102	3	0.3378648135629909
1102	4	0.4398633876662785
1102	5	0.38467539251035987
1102	6	0.38035320025952046
1102	7	0.27837171042983755
1102	8	0.27741902588041967
1102	9	0.45096686806596153
1102	10	0.43918971319900635
1102	11	0.598637565540244
1102	12	0.4345976181859612
1102	13	0.4368787226392712
1102	14	0.5489873137360348
1102	15	0.6019969676238333
1102	16	0.5452685156759998
1102	17	0.6053320364768737
1102	18	0.5234162647687768
1102	19	0.5664519365004068
1102	20	0.47851307597005643
1102	21	0.5287666343260184
1102	22	0.5669204220134247
1102	23	0.615441811867008
1102	24	0.527884734552579
1102	25	0.5235311894346069
1102	26	0.47557944824651344
1102	27	0.5747433294085482
1102	28	0.5001199041823625
1102	29	0.5638044633684842
1102	30	0.5823903620435331
1102	31	0.6077870086707335
1102	32	0.49767114062972695
1102	33	0.6392795665050883
1102	34	0.677265970775693
1102	35	0.5703330516129128
1102	36	0.4297708794156492
1102	37	0.5572763173222128
1102	38	0.5280712850049767
1102	39	0.5262488482587457
1102	40	0.5489974360788068
1102	41	0.5916958783484887
1102	42	0.45841028451231125
1102	43	0.5685119184559326
1102	44	0.6690704587325058
1102	45	0.5605924654390009
1102	46	0.6451626923993246
1102	47	0.6864042414268354
1102	48	0.6711375543619766
1102	49	0.7250279644223809
1102	50	0.6263583495715537
1103	1	0.2537476716638878
1103	2	0.4146588181865276
1103	3	0.2473169850077852
1103	4	0.3376777504252928
1103	5	0.3551550973074029
1103	6	0.2651105093710697
1103	7	0.3859504785252099
1103	8	0.32936339147791927
1103	9	0.2775351337486214
1103	10	0.40626323008802245
1103	11	0.4354243949469989
1103	12	0.3664738600253017
1103	13	0.352859420907798
1103	14	0.4479370724643914
1103	15	0.4058573139196042
1103	16	0.4058986604389858
1103	17	0.4293456612285027
1103	18	0.4076058758956549
1103	19	0.4395714369261796
1103	20	0.5332959314909189
1103	21	0.5387013490577814
1103	22	0.4525292781019843
1103	23	0.6143064158976586
1103	24	0.4288214362272882
1103	25	0.4352541528180822
1103	26	0.4400983242045058
1103	27	0.5105934571405939
1103	28	0.5560381556725673
1103	29	0.49000372536109893
1103	30	0.60197594251472
1103	31	0.4513862138935565
1103	32	0.5107281942771849
1103	33	0.49565197638303005
1103	34	0.48254556203314536
1103	35	0.5283234007471691
1103	36	0.38634198358823113
1103	37	0.5890674044638544
1103	38	0.3237000105229981
1103	39	0.46351822095247286
1103	40	0.5339534394849963
1103	41	0.48553635521479777
1103	42	0.42945768729403255
1103	43	0.6179016431224085
1103	44	0.506992684108603
1103	45	0.46238071409071324
1103	46	0.5724973503612887
1103	47	0.5666199666610083
1103	48	0.6620543866072168
1103	49	0.6075144815952456
1103	50	0.6865343359468028
1104	1	0.47974042540800355
1104	2	0.6532708701664056
1104	3	0.471538233157208
1104	4	0.5022580465915425
1104	5	0.6063808322323041
1104	6	0.6474933809311025
1104	7	0.6071557619285386
1104	8	0.5577437510257752
1104	9	0.5717455238238855
1104	10	0.6101977773101268
1104	11	0.5407692263921859
1104	12	0.603674022283478
1104	13	0.6409110233733699
1104	14	0.7481102685937628
1104	15	0.7641700440130634
1104	16	0.8487533888340906
1104	17	0.8328112604943101
1104	18	0.8250342514782503
1104	19	0.633470826892065
1104	20	0.7407295166397247
1104	21	0.810154310281348
1104	22	0.821185848265003
1104	23	0.8821809342381854
1104	24	0.7617996353750769
1104	25	0.7047864473437215
1104	26	0.7103959591310485
1104	27	0.7846489869879605
1104	28	0.8120904307076131
1104	29	0.7669249516108891
1104	30	0.7869351569181475
1104	31	0.9004052661004991
1104	32	0.9446190205292595
1104	33	0.9654525078109799
1104	34	0.8623096518059796
1104	35	0.9245370497473715
1104	36	0.9278568166159786
1104	37	0.9480117361323508
1104	38	0.9139935296786916
1104	39	0.9040248087562048
1104	40	0.860165925091863
1104	41	0.9569268838960028
1104	42	0.8872238597861184
1104	43	0.9017898634050425
1104	44	0.920503247617269
1104	45	0.8391433914202153
1104	46	0.7657921079654031
1104	47	0.9409581828982638
1104	48	0.889825763920193
1104	49	0.9005481123623582
1104	50	0.9607189383925174
1105	1	0.3673685032517007
1105	2	0.493153533762122
1105	3	0.30915245681187997
1105	4	0.43831528502283135
1105	5	0.3424022800313793
1105	6	0.2626814200603391
1105	7	0.26031046044577977
1105	8	0.16745957866019268
1105	9	0.20552321114634964
1105	10	0.3342513074857507
1105	11	0.380179163114164
1105	12	0.326165807514343
1105	13	0.3440644315115975
1105	14	0.351312309339761
1105	15	0.46441167714147014
1105	16	0.4745916908993024
1105	17	0.5272345205727476
1105	18	0.4109693211517609
1105	19	0.4108781293864183
1105	20	0.430536928731307
1105	21	0.5282150730699726
1105	22	0.4343819130880817
1105	23	0.513675887001817
1105	24	0.5115449980078921
1105	25	0.5406137665063753
1105	26	0.42391381511763626
1105	27	0.46351947110571246
1105	28	0.5671292834787636
1105	29	0.6145698244714737
1105	30	0.5167389868557706
1105	31	0.5588877618387684
1105	32	0.6077316588721828
1105	33	0.5671887707219199
1105	34	0.5182679498470648
1105	35	0.4883517006333946
1105	36	0.451398661289268
1105	37	0.4077023820589929
1105	38	0.4548861041579002
1105	39	0.35780714144311077
1105	40	0.3875495448816082
1105	41	0.27725180596715404
1105	42	0.30803376346820954
1105	43	0.3177647092047522
1105	44	0.3933118711197494
1105	45	0.4025549340845487
1105	46	0.32013174530245436
1105	47	0.2767600035535054
1105	48	0.23769486910957482
1105	49	0.3968635007733923
1105	50	0.42707211250080945
1106	1	0.3577330389143043
1106	2	0.5322286202581736
1106	3	0.38533774187317604
1106	4	0.39261923201138416
1106	5	0.3859144158896015
1106	6	0.34630732361280536
1106	7	0.44826348892322443
1106	8	0.45391382796371715
1106	9	0.4912721043885071
1106	10	0.4486810983743226
1106	11	0.6090150660564433
1106	12	0.4049530322364987
1106	13	0.48876715740085075
1106	14	0.6553276022241832
1106	15	0.5142688300165759
1106	16	0.5733620307907614
1106	17	0.5632254388288587
1106	18	0.5407256932628286
1106	19	0.4382053262586254
1106	20	0.43812736138914943
1106	21	0.4732191576689857
1106	22	0.6321589258573468
1106	23	0.6102163181569722
1106	24	0.5561235921590395
1106	25	0.5405436407262272
1106	26	0.48475202765703784
1106	27	0.4775150285920091
1106	28	0.5365753899952708
1106	29	0.5106784811802783
1106	30	0.684738043345277
1106	31	0.5919393170373226
1106	32	0.5457486657399571
1106	33	0.5989880798463787
1106	34	0.4719407648142706
1106	35	0.44795242083426245
1106	36	0.6384072685457411
1106	37	0.5492849660938148
1106	38	0.5814781642802842
1106	39	0.5909922088412713
1106	40	0.6979818020118955
1106	41	0.6128874506279871
1106	42	0.6983033105740617
1106	43	0.6883790019316152
1106	44	0.5633058694458413
1106	45	0.5288473658735668
1106	46	0.5330522836862954
1106	47	0.5546384163794258
1106	48	0.46257504054433696
1106	49	0.47925901563777984
1106	50	0.6421884074553077
1107	1	0.2640855099242607
1107	2	0.394049795083113
1107	3	0.24068680028325318
1107	4	0.24085809459181462
1107	5	0.21916672093126977
1107	6	0.2836939244450889
1107	7	0.2324945598466499
1107	8	0.20877721661687332
1107	9	0.20220212976756358
1107	10	0.24898503534930683
1107	11	0.22992001237042103
1107	12	0.29069578814546304
1107	13	0.295223509327748
1107	14	0.26653003560230815
1107	15	0.17823406343835302
1107	16	0.315924707130236
1107	17	0.28452929139324823
1107	18	0.21640017658614852
1107	19	0.21939849800007494
1107	20	0.15101265385716545
1107	21	0.2389956307589145
1107	22	0.20969623637099488
1107	23	0.39720632153045443
1107	24	0.27732538731068956
1107	25	0.1939938724436132
1107	26	0.29940150162019563
1107	27	0.3326770307643301
1107	28	0.2701636315695808
1107	29	0.2838795575183397
1107	30	0.40011683075615423
1107	31	0.4148644118963034
1107	32	0.35119194343582194
1107	33	0.26539416187994647
1107	34	0.31774613162243526
1107	35	0.25178645606965944
1107	36	0.25427243158613066
1107	37	0.4170150298252864
1107	38	0.37203311837356523
1107	39	0.40094454185313566
1107	40	0.3734277590824888
1107	41	0.12598829893978802
1107	42	0.08481826169091988
1107	43	0.11947333680404881
1107	44	0.2508612179454346
1107	45	0.38047235811632163
1107	46	0.3730902302205692
1107	47	0.30850064240159014
1107	48	0.44476661024911124
1107	49	0.4627358616931474
1107	50	0.5165404384713455
1108	1	0.3238840176381821
1108	2	0.5089910201181809
1108	3	0.37286837827586794
1108	4	0.3284907820172549
1108	5	0.3810061157406466
1108	6	0.3304544707718833
1108	7	0.29868984874680393
1108	8	0.3472599979369022
1108	9	0.35687600265320185
1108	10	0.36003270883396254
1108	11	0.3462923767730359
1108	12	0.31320534918229015
1108	13	0.3887283472434504
1108	14	0.4364523697422129
1108	15	0.4866013234198121
1108	16	0.5264731940019729
1108	17	0.5144102965978021
1108	18	0.30373095934641725
1108	19	0.3637745970912718
1108	20	0.33405660710189267
1108	21	0.37016450245904053
1108	22	0.3948861039909701
1108	23	0.4397186018043229
1108	24	0.33639481584756936
1108	25	0.4839511607718876
1108	26	0.3425535683776104
1108	27	0.3058818538990522
1108	28	0.4594515714643279
1108	29	0.513187601272918
1108	30	0.6552178926023302
1108	31	0.6287545116628266
1108	32	0.4918240345652265
1108	33	0.5682699212109981
1108	34	0.5808318819389697
1108	35	0.360707937594384
1108	36	0.548911590131091
1108	37	0.5188922858925977
1108	38	0.5897068154190634
1108	39	0.6683049270196264
1108	40	0.6092270782329265
1108	41	0.5871392586411872
1108	42	0.44204199002497546
1108	43	0.46360578821069254
1108	44	0.5487908411432727
1108	45	0.5770720403325811
1108	46	0.6432154379380535
1108	47	0.6359770444955473
1108	48	0.7450695696661569
1108	49	0.7285178034451221
1108	50	0.7469998956151187
1109	1	0.2184670939272486
1109	2	0.31971408936814427
1109	3	0.28771311876528927
1109	4	0.25151971645941984
1109	5	0.2429515938322382
1109	6	0.30608348777851546
1109	7	0.19841118471580188
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2210	46	-0.809091049130835
2210	47	-0.7089378179953201
2210	48	-0.4972089393433876
2210	49	-0.26587470855902495
2210	50	-0.15038582652780264
2211	1	-0.21779006952824886
2211	2	-0.3712884231754927
2211	3	-0.3082676440737657
2211	4	-0.29171893216541633
2211	5	-0.3713458966078136
2211	6	-0.3356987043169255
2211	7	-0.31396300595856985
2211	8	-0.3534970401648394
2211	9	-0.5911735397092244
2211	10	-0.47104367161369964
2211	11	-0.5871291011214907
2211	12	-0.5294982946695868
2211	13	-0.36608976982674335
2211	14	-0.4215170928753551
2211	15	-0.5979401093113736
2211	16	-0.5074606872082867
2211	17	-0.537043156866595
2211	18	-0.46588651838612805
2211	19	-0.5214757987115277
2211	20	-0.3607029624002891
2211	21	-0.3477488478689281
2211	22	-0.42923460006511505
2211	23	-0.32399984133815707
2211	24	-0.29917172477218656
2211	25	-0.3260455590285929
2211	26	-0.37490579449022476
2211	27	-0.2618560176648117
2211	28	-0.5651303081866814
2211	29	-0.31603422638811907
2211	30	-0.2785021100711518
2211	31	-0.43749311803921387
2211	32	-0.34137767976989153
2211	33	-0.24822526477042572
2211	34	-0.23594893781945814
2211	35	-0.4076857356700003
2211	36	-0.3639759228457393
2211	37	-0.29544421773979224
2211	38	-0.30013151600327603
2211	39	-0.3302817567715195
2211	40	-0.2878341376323661
2211	41	-0.3293155588873802
2211	42	-0.4597140727043225
2211	43	-0.549114127728771
2211	44	-0.5369650080437722
2211	45	-0.4531987645885849
2211	46	-0.4312431995306767
2211	47	-0.4954449689360345
2211	48	-0.4995531660538646
2211	49	-0.5376456564213328
2211	50	-0.5101341880399204
2212	1	-0.9396761929776893
2212	2	-2.4405227024931477
2212	3	-1.3309913951456167
2212	4	-0.8254157980926067
2212	5	-0.789217859242676
2212	6	-0.5687173069290665
2212	7	-0.7590800615237754
2212	8	-0.7028641322625191
2212	9	-0.7698846188371817
2212	10	-0.5829554642967238
2212	11	-0.8113279370451849
2212	12	-0.6893180861725483
2212	13	-2.2079670151953934
2212	14	-1.5323753716856885
2212	15	-1.1115749941031083
2212	16	-0.9508944917416216
2212	17	-0.8198615890857539
2212	18	-0.9491575397238492
2212	19	-0.7352940351717692
2212	20	-0.7180302740788278
2212	21	-0.778851632332838
2212	22	-0.7925810850895587
2212	23	-0.7258081299364463
2212	24	-0.6427269825536044
2212	25	-0.7264970452799773
2212	26	-0.7521846627279274
2212	27	-0.788066069747714
2212	28	-0.7716165457828694
2212	29	-0.8216057735177742
2212	30	-0.7354042664522069
2212	31	-0.8047583658672579
2212	32	-0.6978930054879271
2212	33	-0.5387422613322386
2212	34	-0.8021059419910586
2212	35	-0.7425328920648369
2212	36	-0.6738806351214777
2212	37	-0.7524847839518003
2212	38	-0.7993647475971102
2212	39	-0.7079769573692758
2212	40	-0.6796044048846568
2212	41	-0.7040985008407219
2212	42	-0.674429767331425
2212	43	-0.9249762252167102
2212	44	-0.8132495036612539
2212	45	-0.8153781404209042
2212	46	-0.773342534777484
2212	47	-0.8239786243065836
2212	48	-0.8146645142814251
2212	49	-0.7963202582173565
2212	50	-0.8184300928183674
2213	1	-0.8369392735960837
2213	2	-1.810046107271102
2213	3	-1.0445718746717567
2213	4	-0.729555301427574
2213	5	-0.679080789965532
2213	6	-0.6692886266378224
2213	7	-0.5678606041344452
2213	8	-0.5884798832963654
2213	9	-0.6045346323054517
2213	10	-0.6607181687806118
2213	11	-0.5105555703007738
2213	12	-0.5653844868234899
2213	13	-0.5950754410271131
2213	14	-0.7267822026791748
2213	15	-0.7264903673276351
2213	16	-0.7341111466148291
2213	17	-0.6791561589316331
2213	18	-0.680728065293323
2213	19	-0.7203267428238362
2213	20	-0.800195636486869
2213	21	-0.769390488632709
2213	22	-0.9075777564038248
2213	23	-0.8012565953215969
2213	24	-0.7035824757224702
2213	25	-0.7060527041155069
2213	26	-0.8082477556282655
2213	27	-0.7240417654569882
2213	28	-0.7784046920329004
2213	29	-0.843084868394385
2213	30	-0.8063140653493406
2213	31	-0.6525366600449161
2213	32	-0.5465255925536425
2213	33	-0.7174820781358946
2213	34	-0.7528330888537837
2213	35	-0.7827207360115835
2213	36	-0.7390142221885867
2213	37	-0.6611371337806204
2213	38	-0.68181943350735
2213	39	-0.7122723576419929
2213	40	-0.7311795640963226
2213	41	-0.7306378260875164
2213	42	-0.6867053601794311
2213	43	-0.8246199092972191
2213	44	-0.7159037990615289
2213	45	-0.6238924579565861
2213	46	-0.6634963940292096
2213	47	-0.6541342839795234
2213	48	-0.6151158059091841
2213	49	-0.6710520443049859
2213	50	-0.6324467906783795
2214	1	-0.8473930382396588
2214	2	-1.770275534231537
2214	3	-0.9445455372196143
2214	4	-0.63926314208436
2214	5	-0.6015648116618308
2214	6	-0.6933376725241658
2214	7	-0.5546056283489804
2214	8	-0.6282284857857192
2214	9	-0.787501565334999
2214	10	-0.7299441755453628
2214	11	-0.6741591024034221
2214	12	-0.6835380926669217
2214	13	-0.684847041172894
2214	14	-0.9002931605421054
2214	15	-0.9018401174007238
2214	16	-0.9482709553635758
2214	17	-0.7650395158521306
2214	18	-0.8993103485409151
2214	19	-0.891123691542937
2214	20	-0.9339548629276067
2214	21	-0.9109491243581331
2214	22	-0.9557235984125754
2214	23	-0.913872806268266
2214	24	-0.9269857642853125
2214	25	-0.9012253321021291
2214	26	-0.9546190981948541
2214	27	-0.9265118130671013
2214	28	-0.9376796176221163
2214	29	-0.9377356992144854
2214	30	-0.9661761419894219
2214	31	-0.9543410067856399
2214	32	-1.0277556685635858
2214	33	-0.9637284130331949
2214	34	-1.01325621036154
2214	35	-0.9965651347252447
2214	36	-1.0313587166067748
2214	37	-1.0197665061071675
2214	38	-1.113650461882827
2214	39	-1.025638791457105
2214	40	-1.0437354002430865
2214	41	-1.0921813232195774
2214	42	-1.0600456823697257
2214	43	-1.1577537164779557
2214	44	-1.1019015388836877
2214	45	-1.2843082876904006
2214	46	-1.3169493958933742
2214	47	-1.2018156155009763
2214	48	-1.1735904593084499
2214	49	-1.1674957400426536
2214	50	-1.2730659303061884
2215	1	-0.495542984138595
2215	2	-0.2768633498632056
2215	3	-0.5909976198577205
2215	4	-0.49568890733630866
2215	5	-0.461657946388641
2215	6	-0.6330613778907903
2215	7	-0.645000540826608
2215	8	-0.7235146146777041
2215	9	-0.6729697417752476
2215	10	-0.7344377238672166
2215	11	-0.8053249803069349
2215	12	-0.7813037693346985
2215	13	-0.757842520953254
2215	14	-0.9300333237554099
2215	15	-0.819869422985775
2215	16	-0.7704867730986109
2215	17	-0.9430058467888358
2215	18	-0.9076503813049257
2215	19	-0.884819400873603
2215	20	-0.8931119734252349
2215	21	-0.8485450652615683
2215	22	-1.0027351467142083
2215	23	-1.075664183468966
2215	24	-0.9907428555694793
2215	25	-1.048548087919258
2215	26	-1.0778654906146343
2215	27	-1.0198432250542808
2215	28	-1.1443250736384556
2215	29	-1.0757763306295018
2215	30	-1.0090387202337183
2215	31	-1.0125625986714937
2215	32	-1.0349883992009623
2215	33	-0.9133143123748795
2215	34	-1.0012811676368685
2215	35	-1.1193964152518916
2215	36	-0.9608554250374439
2215	37	-0.9802547808394451
2215	38	-0.9614795935354863
2215	39	-0.8623031322836852
2215	40	-0.9577556653888593
2215	41	-1.1219945481485354
2215	42	-1.0084322572162705
2215	43	-0.8206928451980389
2215	44	-0.8330518690518574
2215	45	-0.9520611469568507
2215	46	-0.8021746231683977
2215	47	-0.8457262122306446
2215	48	-0.877615805909187
2215	49	-0.7979347929201239
2215	50	-0.8361705223234992
2216	1	-0.8850065327159236
2216	2	-0.5272429753976304
2216	3	-0.6516593845636045
2216	4	-2.005913918393361
2216	5	-1.1886730945531738
2216	6	-0.914529754715204
2216	7	-0.8368404346788907
2216	8	-0.7830246028835148
2216	9	-0.9576474286394685
2216	10	-0.8984682044805155
2216	11	-1.0582403569839158
2216	12	-0.9684499435938818
2216	13	-1.1344098584145499
2216	14	-1.1964696351220567
2216	15	-1.1790440138747584
2216	16	-1.172164681103915
2216	17	-1.1558108372060545
2216	18	-1.2181407616675983
2216	19	-1.1965739711227528
2216	20	-1.2563656959668776
2216	21	-1.2260964805645287
2216	22	-1.2471550199266517
2216	23	-1.193339806349698
2216	24	-1.2816263142160977
2216	25	-1.3166982427180187
2216	26	-1.4665504382779133
2216	27	-1.5244835022177767
2216	28	-1.5033817226138382
2216	29	-1.3631593101370996
2216	30	-1.392484466953455
2216	31	-1.3169015711025693
2216	32	-1.4375564000855263
2216	33	-1.3951558647853162
2216	34	-1.5412837476162267
2216	35	-1.548039714676925
2216	36	-1.5563054471515514
2216	37	-1.563696841874382
2216	38	-1.6129455746647778
2216	39	-1.6857007808167808
2216	40	-1.7174803447342768
2216	41	-1.6763224635252172
2216	42	-1.7283109981863447
2216	43	-1.5176245126018415
2216	44	-1.4009357331238022
2216	45	-1.3799719165278361
2216	46	-1.3701017940367421
2216	47	-1.3370547173168887
2216	48	-1.334169874905207
2216	49	-1.3447973537339237
2216	50	-1.4489229917323172
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60609.0
3	9201.0
4	16503.0
5	9528.0
6	7250.0
7	5407.0
8	4674.0
9	4102.0
10	4016.0
11	4088.0
12	4216.0
13	4592.0
14	4907.0
15	5319.0
16	5747.0
17	6202.0
18	6725.0
19	7504.0
20	9052.0
21	11199.0
22	14506.0
23	17937.0
24	20871.0
25	23325.0
26	24950.0
27	28384.0
28	33008.0
29	38427.0
30	43875.0
31	51728.0
32	63235.0
33	78658.0
34	100241.0
35	128484.0
36	161024.0
37	211882.0
38	334762.0
39	855191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79810080543727	18.855716481454785	14.311454194498705	25.03472851860924
2	14.278315708612881	21.47324904029422	38.769429008617216	25.479006242475677
3	18.1093633973507	25.93413845824719	30.054821291839744	25.90167685256236
4	11.451458959121226	17.039657933135395	38.86186692085718	32.6470161868862
5	12.328908617948523	39.706793745021365	34.75712543340946	13.207172203620644
6	30.412709927337968	38.537728775511134	17.800483256832127	13.249078040318773
7	26.75767210281138	33.27009800924404	22.244140679867865	17.728089208076717
8	26.158545905194668	34.11864091707116	20.455767840545285	19.267045337188886
9	26.719003812296847	15.59926761596769	20.009270474777203	37.67245809695826
10	14.54946189915515	29.23880936150875	34.287785833798104	21.923942905537995
11	34.12300109280135	23.204448879000655	24.253290282331115	18.419259745866874
12	23.86222129307289	25.816836571538115	30.305729610382524	20.015212525006465
13	27.942934177479817	22.145706987138254	26.333552715567834	23.5778061198141
14	21.73636937193401	21.45041343997012	29.390906841002966	27.422310347092903
15	23.77145085803432	29.353218620270233	24.766692998228404	22.10863752346704
16	22.240769234583162	28.48179298559338	26.964321424498692	22.313116355324762
17	21.812660934984745	27.90377120871057	27.50223945472996	22.781328401574726
18	21.186554481570898	28.194500627436042	28.952382211640764	21.666562679352293
19	22.254800888383862	27.979174629409638	29.00275812199783	20.763266360208668
20	22.792847400495244	26.553511243441168	28.351371016824565	22.302270339239016
21	23.540356425530646	26.46974605786223	28.951114310116076	21.03878320649104
22	22.421776094192623	26.049031663080235	28.447355460137707	23.081836782589438
23	22.106931530881614	26.945380639014253	28.97623766712571	21.971450162978424
24	22.908820772120723	26.328986940162068	28.687723653184843	22.074468634532362
25	21.964327768068518	26.85461313766077	28.77237777695146	22.408681317319253
26	22.63861097120541	26.37235097908053	29.222970390628195	21.766067659085866
27	21.602396740551843	26.714352471421776	29.4130406848444	22.270210103181977
28	21.367463452968344	27.747689754931816	28.868002640988372	22.01684415111147
29	22.084675938301665	27.7231003525513	28.42312105197704	21.769102657169995
30	22.816554014972485	27.188734279717735	28.38331047247608	21.6114012328337
31	22.68735780249363	26.078945204873893	28.62186111979852	22.61183587283396
32	22.068796277896872	27.585396314169365	28.61618786011707	21.72961954781669
33	22.821334562705413	26.141506674796382	28.427742168949433	22.60941659354877
34	22.147688433617287	26.90242917086978	29.10560279838369	21.844279597129244
35	23.316071807726214	26.936282994285353	28.435623294467362	21.31202190352107
36	22.811581127626546	26.54440784445915	28.287822945299133	22.356188082615173
37	22.193339185211958	26.724097691950227	29.68868694170496	21.393876181132853
38	22.90300407626431	26.78165135795615	28.430497793331583	21.884846772447954
39	21.9186314997278	27.515648582850126	28.565303558137057	22.00041635928502
40	23.220237755964344	26.43789821150219	28.63364252677873	21.708221505754732
41	22.052238790558707	27.59191805880757	28.184900487412808	22.17094266322092
42	22.48597473862811	27.677851218028334	27.976921872799355	21.859252170544206
43	22.216720061488367	26.36441769632561	27.826253236420044	23.59260900576598
44	22.4280997094458	26.85443585608171	28.124944501463094	22.592519933009395
45	22.537246057119773	26.460694274021453	27.850340917116394	23.151718751742376
46	22.47300943415318	27.11549826973757	27.83696752459104	22.574524771518217
47	23.00059471693126	26.78741347694646	27.856871458543274	22.355120347579007
48	21.998147286203807	27.34565915269938	27.940543002907926	22.715650558188884
49	23.18935987966924	26.840954088792667	27.69965713001884	22.27002890151925
50	22.423255735195788	26.530063547955844	28.62623336600145	22.420447350846914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	971.0
1	670.5
2	370.0
3	575.0
4	780.0
5	906.5
6	1033.0
7	982.5
8	932.0
9	1222.5
10	1513.0
11	2164.0
12	2815.0
13	5189.0
14	7563.0
15	10374.5
16	13186.0
17	15010.5
18	16835.0
19	17490.5
20	18146.0
21	19657.5
22	21169.0
23	24807.5
24	28446.0
25	34243.0
26	40040.0
27	46846.5
28	53653.0
29	60136.5
30	66620.0
31	73148.0
32	79676.0
33	87503.0
34	95330.0
35	103409.0
36	111488.0
37	117305.0
38	123122.0
39	130213.0
40	137304.0
41	142797.0
42	148290.0
43	148616.5
44	148943.0
45	159824.0
46	170705.0
47	185552.0
48	200399.0
49	191918.5
50	183438.0
51	167147.0
52	150856.0
53	142649.0
54	134442.0
55	126393.0
56	118344.0
57	109192.0
58	100040.0
59	89421.5
60	78803.0
61	68406.5
62	58010.0
63	47958.0
64	37906.0
65	31091.0
66	24276.0
67	20697.0
68	17118.0
69	14662.5
70	12207.0
71	9410.0
72	6613.0
73	5511.5
74	4410.0
75	3289.5
76	2169.0
77	1817.5
78	1466.0
79	1174.5
80	883.0
81	637.0
82	391.0
83	341.0
84	291.0
85	244.5
86	198.0
87	129.5
88	61.0
89	51.5
90	42.0
91	30.0
92	18.0
93	14.0
94	10.0
95	6.5
96	3.0
97	4.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016850250420327017
2	5.781948673641625E-4
3	2.8909743368208123E-4
4	0.001982382402391414
5	0.04068013888240714
6	0.027670754366713485
7	0.030603028336917453
8	0.02391248772884643
9	0.030603028336917453
10	0.03217241440547732
11	0.03898685391369781
12	0.03944114988091251
13	0.0370044715113064
14	0.042373423851116476
15	0.03770656527882002
16	0.043488513952461644
17	0.04530569782132044
18	0.04799017399122548
19	0.04972475859331797
20	0.04617299012236668
21	0.04675118498973085
22	0.04530569782132044
23	0.044190607719975274
24	0.04163003045021969
25	0.039606348414445124
26	0.04328201578554587
27	0.043034217985246946
28	0.04224952495096701
29	0.040143243648426134
30	0.03671537407762431
31	0.03469169204184974
32	0.0314703206379637
33	0.02399508699561274
34	0.02077371559172669
35	0.021930105326455018
36	0.023251693594715957
37	0.018750033555952124
38	0.02003032219082991
39	0.014165774250421979
40	0.007888229976182501
41	0.007351334742201493
42	0.006690540608071022
43	0.0023953787362229586
44	0.003882165538016519
45	0.002725775803288194
46	8.259926676630892E-4
47	3.716967004483901E-4
48	6.607941341304713E-4
49	1.2389890014946338E-4
50	3.3039706706523565E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2421329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.18673683141011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.31095797666457	35.07553323193945
2	13.880283863438267	13.65451739072413
3	5.792814601952465	8.547889420181566
4	2.971723068900563	5.846774421033696
5	1.698072816843557	4.1761330381327655
6	1.0991753805329554	3.2438910104303718
7	0.7121550608968247	2.4520008494442274
8	0.500559618273224	1.969671536994894
9	0.353894969858024	1.5666244873529795
>10	1.5308735395713	12.84946866310454
>50	0.0974331213080162	3.311240214876343
>100	0.0491723978987668	4.258324776986912
>500	0.0011873580606524448	0.41644988998271987
>1k	0.0014417919307911226	1.6783143317414908
>5k	1.6962258009307326E-4	0.47593677199340495
>10k+	8.481129004653663E-5	0.4772299650805989
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11440	0.472467805903287	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	6201	0.2560990266089408	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5208	0.21508849065946845	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	4287	0.17705152831358317	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4210	0.17387145654308025	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	3271	0.1350911007962982	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	3170	0.13091983782459962	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	2967	0.12253601224781928	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	2930	0.12100792581264255	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2744	0.11332619400337583	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2477	0.10229919189007358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.1299633383154456E-5	0.0
2	0.0	0.0	0.0	4.1299633383154456E-5	0.0
3	0.0	0.0	0.0	4.1299633383154456E-5	0.0
4	0.0	0.0	0.0	4.1299633383154456E-5	0.0
5	0.0	0.0	0.0	4.1299633383154456E-5	0.0
6	0.0	0.0	0.0	4.1299633383154456E-5	0.0
7	0.0	0.0	0.0	4.1299633383154456E-5	0.0
8	0.0	0.0	0.0	4.1299633383154456E-5	0.0
9	0.0	0.0	0.0	4.1299633383154456E-5	0.0
10	0.0	0.0	0.0	4.1299633383154456E-5	0.0
11	0.0	0.0	0.0	4.1299633383154456E-5	0.0
12	0.0	0.0	0.0	4.1299633383154456E-5	0.0
13	0.0	0.0	0.0	4.1299633383154456E-5	0.0
14	0.0	0.0	0.0	4.1299633383154456E-5	0.0
15	0.0	0.0	0.0	4.1299633383154456E-5	0.0
16	0.0	0.0	0.0	4.1299633383154456E-5	0.0
17	0.0	0.0	0.0	4.1299633383154456E-5	0.0
18	0.0	0.0	0.0	8.259926676630891E-5	0.0
19	0.0	0.0	0.0	8.259926676630891E-5	0.0
20	0.0	0.0	0.0	8.259926676630891E-5	0.0
21	0.0	0.0	0.0	1.6519853353261782E-4	0.0
22	0.0	0.0	0.0	1.6519853353261782E-4	0.0
23	0.0	0.0	0.0	3.716967004483901E-4	0.0
24	0.0	0.0	0.0	6.607941341304713E-4	0.0
25	0.0	0.0	0.0	6.607941341304713E-4	0.0
26	0.0	0.0	0.0	9.91191201195707E-4	0.0
27	0.0	0.0	0.0	0.0012802886348777882	0.0
28	0.0	0.0	0.0	0.0020649816691577227	0.0
29	0.0	0.0	0.0	0.0028496747034376575	0.0
30	0.0	0.0	0.0	0.004914656372595381	0.0
31	0.0	0.0	0.0	0.009870612378573916	0.0
32	0.0	0.0	0.0	0.01511566581823453	0.0
33	0.0	0.0	0.0	0.020649816691577227	0.0
34	0.0	0.0	0.0	0.028909743368208122	0.0
35	0.0	0.0	0.0	0.03630237774379277	0.0
36	0.0	0.0	0.0	0.04427320698674158	0.0
37	0.0	0.0	0.0	0.05864547940407933	0.0
38	0.0	0.0	0.0	0.07429804045629487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	155	1.8189894E-12	21.288963	30
CTAGCGG	175	9.094947E-12	18.856718	29
CGCAATA	160	1.1041266E-9	17.87312	36
CCGGTCA	140	8.376628E-8	17.286753	21
TCGCGTA	180	2.8558134E-10	17.111431	9
CGCTTCG	90	2.2179584E-4	17.109665	32
CGTACAC	120	6.4044325E-6	16.502014	3
ACCGTGC	230	1.8189894E-12	16.261175	8
GCGTAAC	205	1.05501385E-10	16.099194	11
CGCGTAA	195	8.931238E-10	15.795821	10
TCGTTTA	185	7.592462E-9	15.457195	38
GTACATG	8785	0.0	15.255064	1
TACATGG	8535	0.0	15.235603	2
CGATACT	145	2.2838722E-6	15.174893	4
AACCGTG	145	2.28731E-6	15.172699	7
TCGTATG	440	0.0	14.996255	40
ATGCCGT	460	0.0	14.821467	44
ATACGAT	135	1.9526515E-5	14.670578	1
TACGACG	165	5.413367E-7	14.669063	5
CCGACAT	150	3.248757E-6	14.668759	14
>>END_MODULE