FastQCFastQC Report
Mon 6 Feb 2017
SRR2077436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767296
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40600.025.0368921
GGTATCA18450.024.8045541
TATCAAC54600.018.4140262
TCAACGC54950.018.09594
ATCAACG55900.017.866433
CAACGCA56100.017.6844585
AACGCAG58200.016.8186666
GCGTTAT953.3458092E-416.2120881
GTACATG71850.015.5255021
GTATATA7450.015.35718251
TACATGG72300.015.1536132
GAGTACT35200.015.12563512
GTACTAG2850.014.6680771
ACGCAGA66750.014.59872157
AGTACTT38300.014.24602813
ACATGGG75350.014.1888363
CGCAGAG69400.014.0412768
CTAGCGA950.00624377213.8943154
AGAGTAC64450.013.893270511
GTACTTT37850.013.6614
Produced by FastQC (version 0.11.5)