FastQCFastQC Report
Mon 6 Feb 2017
SRR2077436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767296
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.10963771132542381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.025.6934261
GTATCAA48250.023.8030321
TATACCG1203.1610398E-718.3358655
TCAACGC68400.016.5984824
TATCAAC67800.016.582212
ATCAACG68600.016.5497913
CAACGCA69400.016.2961985
GTGCCGA2201.8189894E-1116.0013411
AACGCAG71600.015.855796
GTACATG69250.013.5347081
ACGCAGA85400.013.2160857
TACATGG70900.013.095362
GTAATAC3203.6379788E-1213.063833
ACCGTGC4550.013.0555678
CGCAGAG86450.013.0301198
CGTGCCG2207.386734E-813.00108910
GTATTAT5600.012.9653731
GAGTACT53000.012.95202912
TAGACGG1200.00196071512.8351065
TATACTG6350.012.8206685
Produced by FastQC (version 0.11.5)