##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077436_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2767296 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.375096484076874 31.0 30.0 34.0 26.0 34.0 2 30.308759164180486 31.0 30.0 34.0 26.0 34.0 3 30.486260956543862 31.0 31.0 34.0 26.0 34.0 4 33.97025218841787 37.0 35.0 37.0 30.0 37.0 5 33.5933796023266 37.0 35.0 37.0 28.0 37.0 6 33.5364279787923 37.0 35.0 37.0 28.0 37.0 7 33.33532733758875 37.0 35.0 37.0 27.0 37.0 8 33.29545592520641 37.0 35.0 37.0 27.0 37.0 9 34.72180316091954 39.0 35.0 39.0 27.0 39.0 10 34.57011826707371 39.0 35.0 39.0 27.0 39.0 11 34.53455214042878 39.0 35.0 39.0 27.0 39.0 12 34.45643436770045 39.0 35.0 39.0 26.0 39.0 13 34.43423508002035 39.0 35.0 39.0 26.0 39.0 14 35.56331487488147 40.0 36.0 41.0 26.0 41.0 15 35.570730055621084 40.0 36.0 41.0 27.0 41.0 16 35.508214878350564 40.0 36.0 41.0 26.0 41.0 17 35.47239615856056 40.0 36.0 41.0 26.0 41.0 18 35.463327739425054 40.0 36.0 41.0 26.0 41.0 19 35.49452823261407 40.0 35.0 41.0 25.0 41.0 20 35.489947949189386 40.0 35.0 41.0 26.0 41.0 21 35.4271198310553 40.0 35.0 41.0 25.0 41.0 22 35.4347850753949 40.0 35.0 41.0 25.0 41.0 23 35.367912214667314 40.0 35.0 41.0 25.0 41.0 24 35.3516450715789 40.0 35.0 41.0 25.0 41.0 25 35.332738528874394 40.0 35.0 41.0 25.0 41.0 26 35.25297113138602 39.0 35.0 41.0 25.0 41.0 27 35.19017914961031 39.0 35.0 41.0 25.0 41.0 28 35.12091731423021 39.0 35.0 41.0 24.0 41.0 29 35.0393279215523 39.0 35.0 41.0 24.0 41.0 30 34.90741142255834 39.0 34.0 41.0 24.0 41.0 31 34.82314540981521 39.0 34.0 41.0 23.0 41.0 32 34.74428142128634 39.0 34.0 41.0 23.0 41.0 33 34.648070896644235 39.0 34.0 41.0 22.0 41.0 34 34.62251815490645 39.0 34.0 41.0 22.0 41.0 35 34.56335390214852 39.0 34.0 41.0 22.0 41.0 36 34.455969654131685 39.0 34.0 41.0 21.0 41.0 37 34.364107417493464 39.0 34.0 41.0 19.0 41.0 38 34.27773718460186 39.0 34.0 41.0 19.0 41.0 39 34.18168746675455 39.0 34.0 41.0 18.0 41.0 40 34.08822836443951 39.0 33.0 41.0 16.0 41.0 41 34.023787119267325 39.0 33.0 41.0 16.0 41.0 42 33.91321745125928 39.0 33.0 41.0 15.0 41.0 43 34.0029335495733 39.0 33.0 41.0 15.0 41.0 44 33.94117181537501 39.0 33.0 41.0 14.0 41.0 45 33.87444855917112 39.0 33.0 41.0 9.0 41.0 46 33.718626775017924 39.0 33.0 41.0 7.0 41.0 47 33.5697525671269 39.0 33.0 41.0 2.0 41.0 48 33.40717183850228 39.0 33.0 41.0 2.0 41.0 49 33.24991363410347 39.0 33.0 41.0 2.0 41.0 50 33.103167134993875 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6284340185875159 1101 2 0.7442062046765479 1101 3 0.6163841442736917 1101 4 0.6478848017590408 1101 5 0.6334809893255269 1101 6 0.7360044752882544 1101 7 0.7771114408508026 1101 8 0.8132363812295438 1101 9 0.8226728121558722 1101 10 0.8236521656145186 1101 11 0.9562251220598483 1101 12 0.9878390807456441 1101 13 0.9336627678253748 1101 14 1.0194100759831741 1101 15 0.9527075451516893 1101 16 0.9537567768710815 1101 17 0.9627364335435331 1101 18 0.9820175152775619 1101 19 0.8892050647647594 1101 20 0.9225031817467482 1101 21 0.990780614493147 1101 22 0.8904263030787263 1101 23 1.0215229040042288 1101 24 1.0010808863669567 1101 25 0.9337032479403575 1101 26 0.8186929684993203 1101 27 0.9244146318708104 1101 28 0.9797771894808704 1101 29 1.0115064299338812 1101 30 1.0122923834325732 1101 31 1.095515091512489 1101 32 1.0721477076195143 1101 33 1.1204086487633802 1101 34 0.8846606785880979 1101 35 0.9649097631720664 1101 36 0.9296016520560357 1101 37 1.0521585504505282 1101 38 1.0114677680816655 1101 39 1.0358404442570261 1101 40 1.0496856266297812 1101 41 1.0116654296732648 1101 42 1.125048575176379 1101 43 1.0448114197751721 1101 44 1.073955475657499 1101 45 1.0977961709570039 1101 46 1.0627707289290313 1101 47 1.1385769103935246 1101 48 1.2006200026632854 1101 49 1.145190247152179 1101 50 1.1621865699684903 1102 1 0.6700861904162316 1102 2 0.8105550060542797 1102 3 0.6887366546137272 1102 4 0.7581095276671661 1102 5 0.7898339387869271 1102 6 0.7099467754501347 1102 7 0.7943099987066731 1102 8 0.867051688590756 1102 9 0.8918975266797915 1102 10 0.9166529337336655 1102 11 0.9681419240069005 1102 12 1.0172722257476252 1102 13 0.8640488164887827 1102 14 1.0589624628017305 1102 15 1.0148757120448053 1102 16 1.0392663381438751 1102 17 1.0663620394449183 1102 18 0.9510849733237663 1102 19 1.064960817177429 1102 20 1.0784674664660372 1102 21 1.1237398823252036 1102 22 1.133748280239267 1102 23 1.1046336017752836 1102 24 1.0235626303080565 1102 25 1.02870414637718 1102 26 1.01185459714074 1102 27 1.0111960204245136 1102 28 1.0444392407966276 1102 29 1.0772807580498736 1102 30 1.0858461275457572 1102 31 1.0325651901917965 1102 32 1.1051362711852164 1102 33 1.1839552768907993 1102 34 1.181347171504349 1102 35 1.1802786562973324 1102 36 1.168185855208364 1102 37 1.1641223136702905 1102 38 1.2103130322040698 1102 39 1.2302017048740126 1102 40 1.2925081861867653 1102 41 1.2834231468050774 1102 42 1.17047515418124 1102 43 1.1497224682369307 1102 44 1.1944465227245473 1102 45 1.1701804312134172 1102 46 1.2835006669434676 1102 47 1.3641177902414867 1102 48 1.4333910535586298 1102 49 1.354816123871771 1102 50 1.3577793268915315 1103 1 0.4310880122506724 1103 2 0.5678025341077522 1103 3 0.48148533637131763 1103 4 0.4345904754529428 1103 5 0.4970640873685497 1103 6 0.597952825733536 1103 7 0.5864355745341641 1103 8 0.5904502574538597 1103 9 0.6424938243011411 1103 10 0.6029493120371399 1103 11 0.6689009962523116 1103 12 0.6425673644529937 1103 13 0.6106254223121326 1103 14 0.8370779627751332 1103 15 0.773168382576884 1103 16 0.781715001433831 1103 17 0.8380425278179686 1103 18 0.8073714666110021 1103 19 0.8054808650157739 1103 20 0.7094914149769025 1103 21 0.8796911714162192 1103 22 0.7939514829800061 1103 23 0.8491612444626142 1103 24 0.7849344265879736 1103 25 0.7428634160515841 1103 26 0.761839490086416 1103 27 0.8259132703516059 1103 28 0.8381378156912405 1103 29 0.8174070332392986 1103 30 0.9393285028280474 1103 31 0.9658984519232519 1103 32 0.8371040587058118 1103 33 0.8988933865819746 1103 34 1.0013134798054963 1103 35 0.9576563554361428 1103 36 0.8914552015225894 1103 37 0.8679267080396471 1103 38 0.7783725871799234 1103 39 0.84737916898154 1103 40 0.8890639134674885 1103 41 0.8478843116700432 1103 42 0.7613098724927596 1103 43 0.8634208102370096 1103 44 0.831727646379619 1103 45 0.9052903164501132 1103 46 1.0626182917403142 1103 47 1.075497248864579 1103 48 1.0478864431795998 1103 49 0.9857976765332666 1103 50 0.8841306366491182 1104 1 0.4941998634979399 1104 2 0.6520082103622649 1104 3 0.5338806790567965 1104 4 0.5849275833032905 1104 5 0.5953925503931146 1104 6 0.5424318046915388 1104 7 0.5713218190660143 1104 8 0.6187604654126062 1104 9 0.6764951870563252 1104 10 0.7363192752365464 1104 11 0.6846780028516761 1104 12 0.7627152652978069 1104 13 0.8028263428925086 1104 14 0.7898794269054648 1104 15 0.8310789369672449 1104 16 0.8499446680333165 1104 17 0.8818933777439426 1104 18 0.7753907886436266 1104 19 0.7927708890757401 1104 20 0.8588613347171403 1104 21 0.7896549827936994 1104 22 0.8389677480152642 1104 23 0.9086039560837946 1104 24 0.8377395825298706 1104 25 0.7326011776962531 1104 26 0.7802129045198711 1104 27 0.8586579759279758 1104 28 0.9841210803657532 1104 29 0.9460892272441086 1104 30 1.0083761329621908 1104 31 0.9372129097890394 1104 32 0.8490498407390419 1104 33 0.9825818766221772 1104 34 0.9401213151378371 1104 35 1.0325338327470277 1104 36 1.0275705199684992 1104 37 1.0292433521674198 1104 38 0.9841972347782217 1104 39 0.9947336145989425 1104 40 0.8962892765011716 1104 41 0.9427722391358131 1104 42 1.0030478642936131 1104 43 1.0749290183647204 1104 44 1.058896508066617 1104 45 0.9893321893537887 1104 46 1.0217998825275032 1104 47 0.9738602907487959 1104 48 1.1030018638432324 1104 49 1.1343972493474297 1104 50 1.1559498527389422 1105 1 0.4680466049917378 1105 2 0.5846918665753336 1105 3 0.4818732094022664 1105 4 0.5295979456207078 1105 5 0.6119087139681056 1105 6 0.5726045730996034 1105 7 0.6695787908523982 1105 8 0.591820738472876 1105 9 0.6376954247271485 1105 10 0.5799273011975146 1105 11 0.6913536801155828 1105 12 0.6917590972828194 1105 13 0.6908141720568111 1105 14 0.780742158944669 1105 15 0.7813365225189486 1105 16 0.7482679134153827 1105 17 0.707029679350839 1105 18 0.780413543666377 1105 19 0.7907268546787662 1105 20 0.7951929769311334 1105 21 0.7158999796269327 1105 22 0.7560395650870859 1105 23 0.71706542925115 1105 24 0.6826805892355878 1105 25 0.7012658463609256 1105 26 0.7579988440705137 1105 27 0.8394458155393423 1105 28 0.8040093620187392 1105 29 0.8272058230665849 1105 30 0.7905377621826446 1105 31 0.777383523489064 1105 32 0.8055361648724286 1105 33 0.8248128482649122 1105 34 0.8216071495648478 1105 35 0.8172445468695031 1105 36 0.8968143480358535 1105 37 0.7839383303918055 1105 38 0.8668116791573652 1105 39 0.8341213510455034 1105 40 0.8277659697425719 1105 41 0.7749893684117666 1105 42 0.7481312123181354 1105 43 0.7806350770119508 1105 44 0.7605207008672821 1105 45 0.8116663910997559 1105 46 0.7743613733242896 1105 47 0.7898772802951939 1105 48 0.7405231660704175 1105 49 0.8616141500937431 1105 50 0.8910291525241263 1106 1 0.4117996967661206 1106 2 0.5391437789305087 1106 3 0.47840950501285207 1106 4 0.49866385618164344 1106 5 0.46994360509718547 1106 6 0.33751486916009554 1106 7 0.4852819028295201 1106 8 0.3245329387157412 1106 9 0.5218748725261335 1106 10 0.48525478247963605 1106 11 0.46241580475327027 1106 12 0.4943409480479559 1106 13 0.4018893731806088 1106 14 0.4567251206716705 1106 15 0.5129190416737899 1106 16 0.6232551956695005 1106 17 0.6159011365212947 1106 18 0.6353870049809629 1106 19 0.5562727952767759 1106 20 0.5311178173456739 1106 21 0.586301914342684 1106 22 0.6321024206917158 1106 23 0.5254785081108864 1106 24 0.5321577725475279 1106 25 0.5792471260547671 1106 26 0.6159362010127296 1106 27 0.5724762965911125 1106 28 0.5277015380830505 1106 29 0.6304075661665038 1106 30 0.6417756317727452 1106 31 0.5972947393271468 1106 32 0.6769474813196155 1106 33 0.7482349711547513 1106 34 0.6489559405563128 1106 35 0.5846873331968112 1106 36 0.6639615108321735 1106 37 0.684752643749512 1106 38 0.657706096781709 1106 39 0.6942636387508543 1106 40 0.7114283460918216 1106 41 0.7477489007871299 1106 42 0.7236017419427938 1106 43 0.6571523728766238 1106 44 0.5781747663112071 1106 45 0.5297205193367134 1106 46 0.5397774121955479 1106 47 0.5947187629658828 1106 48 0.6397509305145661 1106 49 0.7597593821663082 1106 50 0.8020247294997986 1107 1 0.38028662235354105 1107 2 0.5850533762243444 1107 3 0.43325798821705064 1107 4 0.5136516186340927 1107 5 0.44474087914718297 1107 6 0.40649919354100206 1107 7 0.43439726397954814 1107 8 0.3785568947970148 1107 9 0.2928106562594124 1107 10 0.46818622339679195 1107 11 0.4514134700865995 1107 12 0.42730234605165407 1107 13 0.4762078132437537 1107 14 0.5426801754012374 1107 15 0.44899188862639505 1107 16 0.5734036851480155 1107 17 0.5786073163734997 1107 18 0.5187586646657252 1107 19 0.41281751915504117 1107 20 0.5770887880780293 1107 21 0.57577012693163 1107 22 0.5180007695249529 1107 23 0.6566375499751018 1107 24 0.5912437993011892 1107 25 0.6295313588285794 1107 26 0.48920005048228177 1107 27 0.5193379091805781 1107 28 0.536058229760755 1107 29 0.5196885660615038 1107 30 0.6140214565745978 1107 31 0.5693997476473314 1107 32 0.5087952701347191 1107 33 0.5335578478589937 1107 34 0.5642426838461816 1107 35 0.5387398055375385 1107 36 0.5177862587414381 1107 37 0.6133892952399975 1107 38 0.518051864724896 1107 39 0.5384053438566951 1107 40 0.5399578601042734 1107 41 0.39140460201343075 1107 42 0.4374671817588194 1107 43 0.43129938349233043 1107 44 0.603721183516889 1107 45 0.5251352122853987 1107 46 0.5353547315860254 1107 47 0.5546215200451243 1107 48 0.5589293292978894 1107 49 0.5159851906928736 1107 50 0.6133984972699764 1108 1 0.30716401198431953 1108 2 0.49542849112563303 1108 3 0.3490752898673115 1108 4 0.3622357224071777 1108 5 0.2956840177525848 1108 6 0.25746343459912424 1108 7 0.37798612468424153 1108 8 0.25283245125145726 1108 9 0.33775522419034587 1108 10 0.2171133673877108 1108 11 0.43529986701725676 1108 12 0.3101473787275495 1108 13 0.37100043829172336 1108 14 0.270806569064149 1108 15 0.35573753836099087 1108 16 0.3687490690961752 1108 17 0.41500376125791405 1108 18 0.4043690588564033 1108 19 0.4616977989861013 1108 20 0.3872322669970458 1108 21 0.5233912798213041 1108 22 0.44871300759989907 1108 23 0.626830178804795 1108 24 0.5337382053230328 1108 25 0.3784741207238582 1108 26 0.4652337233109023 1108 27 0.44489430616452097 1108 28 0.4158838879822966 1108 29 0.4788746987831871 1108 30 0.5340389298233248 1108 31 0.6624141880975145 1108 32 0.4878041798618753 1108 33 0.5839526987769545 1108 34 0.5368970913807161 1108 35 0.6013063318032508 1108 36 0.6073527464586093 1108 37 0.5599710478655524 1108 38 0.5749415178057902 1108 39 0.601282138857961 1108 40 0.5666499555746967 1108 41 0.5718700615292676 1108 42 0.5624849020733862 1108 43 0.49520763922104294 1108 44 0.6416415075502684 1108 45 0.5877800892419103 1108 46 0.5657103486567436 1108 47 0.45469069848171983 1108 48 0.5381981543833092 1108 49 0.48556281883359986 1108 50 0.5195063201476913 1109 1 0.25188892301127197 1109 2 0.3813901050457247 1109 3 0.34351494319589904 1109 4 0.27993284279104813 1109 5 0.26037585863777934 1109 6 0.33421975117047253 1109 7 0.3900355492838443 1109 8 0.3778760333889295 1109 9 0.36305023868956 1109 10 0.3242281480300022 1109 11 0.2957619300934553 1109 12 0.2876282070759757 1109 13 0.3796784723135147 1109 14 0.33594265465149675 1109 15 0.4425061545746587 1109 16 0.46552618257238976 1109 17 0.426654358469186 1109 18 0.42768863144779345 1109 19 0.4194719492466419 1109 20 0.3606498828943714 1109 21 0.3062661535374076 1109 22 0.3499199463092353 1109 23 0.35825040461333657 1109 24 0.24666217726780815 1109 25 0.28671921156112745 1109 26 0.31231119838286503 1109 27 0.24616736403304174 1109 28 0.2449022244305894 1109 29 0.2889891873815955 1109 30 0.3199556017270666 1109 31 0.3948748928791659 1109 32 0.2459775799973869 1109 33 0.23668325633785514 1109 34 0.1495968813520463 1109 35 0.12539715375628901 1109 36 0.06625166177696684 1109 37 0.140640699752403 1109 38 0.11927103414836893 1109 39 0.13088155286899195 1109 40 0.2063827116504484 1109 41 0.2592624792671572 1109 42 0.06706249200890824 1109 43 0.07394008424100917 1109 44 0.08114564554285408 1109 45 0.07109809863273142 1109 46 -0.012461086915891428 1109 47 0.18734268408971388 1109 48 0.11983920867759679 1109 49 0.03479582074376708 1109 50 -0.10880266123174209 1110 1 0.1572966345771185 1110 2 0.20891431389978266 1110 3 0.14608684082595857 1110 4 0.22148861976569378 1110 5 0.15875784372361323 1110 6 0.206630367209236 1110 7 0.21451480500127218 1110 8 0.12521465398137366 1110 9 0.1601735831158848 1110 10 0.2868077208865003 1110 11 0.2759055511602355 1110 12 0.20395033718714473 1110 13 0.1918450461663994 1110 14 0.19191370747066827 1110 15 0.22814656700015945 1110 16 0.2028427904867698 1110 17 0.23270642458975743 1110 18 0.22103106928623362 1110 19 0.330044252077208 1110 20 0.3095921005524289 1110 21 0.3470959998774461 1110 22 0.23418514253400957 1110 23 0.29639301053494904 1110 24 0.26414732929571016 1110 25 0.3095191363096035 1110 26 0.24120563882831192 1110 27 0.3198362991436099 1110 28 0.28008472277038976 1110 29 0.24487442988284158 1110 30 0.17111227228672732 1110 31 0.2754103685582976 1110 32 0.2595941391912291 1110 33 0.33484506104515077 1110 34 0.17749681183551047 1110 35 0.2793933901082397 1110 36 0.3478065168668678 1110 37 0.3639124472546058 1110 38 0.2022596344003844 1110 39 0.4114287664991636 1110 40 0.4407835374876612 1110 41 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28 -1.2164211878235776 2215 29 -1.3446850311772067 2215 30 -1.0593319759223903 2215 31 -1.1220200141497685 2215 32 -1.095128856380832 2215 33 -1.2191813927881512 2215 34 -1.193736330484512 2215 35 -1.2637097929083652 2215 36 -1.3094725023686422 2215 37 -1.2197295703349198 2215 38 -1.3137450190463866 2215 39 -1.2232007386295294 2215 40 -1.3290967753554668 2215 41 -1.3459622290104178 2215 42 -1.405043064385552 2215 43 -1.2987835435831414 2215 44 -1.2145718536370396 2215 45 -1.2087573028533498 2215 46 -1.162872960968869 2215 47 -1.1834944460177113 2215 48 -1.2204736802205502 2215 49 -1.1492592901914591 2215 50 -1.225095898895045 2216 1 -1.5303310364290468 2216 2 -1.1332921407372432 2216 3 -1.3139994822835455 2216 4 -2.6671087771518813 2216 5 -1.972369487619261 2216 6 -1.8825920981552784 2216 7 -1.7036217640178002 2216 8 -1.6621214048233632 2216 9 -1.8412688413701233 2216 10 -1.89770269369059 2216 11 -2.032128505301756 2216 12 -1.9355861725439425 2216 13 -1.9927518151854642 2216 14 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-2.4278260795555155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74469.0 3 11446.0 4 19830.0 5 11447.0 6 8753.0 7 6742.0 8 5752.0 9 5186.0 10 4832.0 11 4796.0 12 5106.0 13 5338.0 14 5724.0 15 6363.0 16 6769.0 17 7331.0 18 7987.0 19 8685.0 20 10391.0 21 13000.0 22 16150.0 23 19349.0 24 22690.0 25 25567.0 26 28281.0 27 32601.0 28 37566.0 29 43513.0 30 50347.0 31 59475.0 32 73146.0 33 89949.0 34 112914.0 35 143722.0 36 185934.0 37 248767.0 38 390542.0 39 956836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.0394870186101 18.52799033136192 14.213714778068038 26.21880787195994 2 14.940127106807807 20.152720415975402 38.03873769142902 26.86841478578777 3 17.474069387042697 24.80695005111501 29.774302575553186 27.944677986289108 4 12.416256902628357 16.773084257508287 36.70545015381735 34.105208686046 5 13.68820193138113 37.79019429854671 34.439902726007126 14.081701044065031 6 32.400348161524974 37.09792621231202 17.1327134477389 13.369012178424105 7 27.990970409837846 32.029235918827666 21.858837648113475 18.12095602322101 8 26.72660949976795 34.69708338935805 19.681916956187948 18.894390154686054 9 26.62651181637741 15.07090115624425 18.901504704392657 39.40108232298568 10 14.2622957930679 28.888308920436923 33.932419858923076 22.9169754275721 11 35.21835761296372 22.626256126542795 23.46204706601953 18.693339194473946 12 23.396257654362447 24.62384766576062 30.39725865463972 21.58263602523721 13 28.279635816531695 21.60649358818175 26.03284244864436 24.08102814664219 14 22.345164815659015 20.604117418029603 27.16511137678312 29.885606389528263 15 23.707315697730323 28.778461322677458 23.7419829678509 23.772240011741324 16 23.72305258469601 27.713034248595868 25.162176153815007 23.40173701289311 17 22.57367468714356 27.600816328006232 26.61008768837817 23.215421296472037 18 22.65851469906629 27.094771504122622 27.51759065032802 22.72912314648306 19 23.49539028887523 26.675512491413283 27.6019017318052 22.227195487906286 20 23.64461441182117 26.07807388302547 27.571374186963872 22.705937518189486 21 23.845353467356087 25.989751257954634 27.46751805315839 22.69737722153089 22 23.551756164099295 25.747372523415102 27.41161877719801 23.289252535287595 23 23.348342512974114 25.734675543231678 27.41177512621629 23.50520681757791 24 23.126808914300753 26.00747895459485 27.39177965715674 23.473932473947656 25 23.33829076377928 25.879919803862165 27.495302250220337 23.286487182138213 26 23.217403155829523 26.11098799437045 27.476547465962238 23.19506138383779 27 23.23395991225292 26.26356948981466 27.332535106784494 23.16993549114793 28 23.236376217085418 26.146897279491217 27.3198688278997 23.296857675523665 29 23.368900752728834 26.300284650217158 27.196784616597053 23.13402998045696 30 23.47582533102169 26.19174314712445 27.237731194627884 23.094700327225983 31 23.46031843576429 25.852183484646652 27.312021139883637 23.375476939705422 32 23.390131455758844 26.216650773603007 27.131628546207608 23.261589224430544 33 23.434655817295667 25.960416567267114 27.17605504494306 23.428872570494164 34 23.512022230029334 26.053526429243707 27.285279111492795 23.149172229234168 35 23.44468934268557 26.18316814147777 27.15053627413631 23.221606241700346 36 23.55083084625847 26.175605979389417 27.007048629798557 23.266514544553548 37 23.561268739306154 25.87262151137533 27.282022627041275 23.284087122277246 38 23.592809869532232 26.032000873230814 27.091841400176452 23.2833478570605 39 23.619608398725504 26.259967848448223 26.965596104804806 23.154827648021463 40 23.636619896461063 25.96550509111607 27.18267484618234 23.215200166240525 41 23.340762292928442 26.146480483712363 27.01901883524948 23.493738388109715 42 23.663601023302576 26.127716227599894 26.895012466034167 23.31367028306336 43 23.428134272561405 25.958203074041002 27.0701786655787 23.5434839878189 44 23.474080849204665 26.152888785455048 26.821109127959986 23.5519212373803 45 23.543745455754404 26.225988676065214 26.691435063724235 23.538830804456143 46 23.631769691455034 25.99300612623447 26.602088188433477 23.773135993877016 47 23.582821340992318 26.187062162531337 26.611989907830534 23.618126588645804 48 23.468003138091568 26.393829046282512 26.586183507529213 23.551984308096703 49 23.657153883902467 26.156599190400442 26.58640534760672 23.599841578090366 50 23.704086481564126 26.25285703186686 26.46862730572635 23.574429180842664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 501.0 1 382.0 2 263.0 3 408.5 4 554.0 5 625.0 6 696.0 7 681.0 8 666.0 9 841.0 10 1016.0 11 1514.0 12 2012.0 13 3877.0 14 5742.0 15 7784.5 16 9827.0 17 11382.5 18 12938.0 19 14039.5 20 15141.0 21 16594.5 22 18048.0 23 20963.0 24 23878.0 25 29112.0 26 34346.0 27 40354.0 28 46362.0 29 52938.0 30 59514.0 31 66832.5 32 74151.0 33 83255.0 34 92359.0 35 101385.0 36 110411.0 37 118813.0 38 127215.0 39 135288.0 40 143361.0 41 151512.5 42 159664.0 43 165867.5 44 172071.0 45 179662.5 46 187254.0 47 191690.5 48 196127.0 49 196918.0 50 197709.0 51 196674.0 52 195639.0 53 189788.0 54 183937.0 55 175888.0 56 167839.0 57 156423.0 58 145007.0 59 131467.0 60 117927.0 61 103932.0 62 89937.0 63 77160.5 64 64384.0 65 53621.5 66 42859.0 67 35040.5 68 27222.0 69 22076.5 70 16931.0 71 13305.0 72 9679.0 73 8042.0 74 6405.0 75 4951.0 76 3497.0 77 2730.0 78 1963.0 79 1515.5 80 1068.0 81 806.5 82 545.0 83 430.5 84 316.0 85 239.5 86 163.0 87 115.0 88 67.0 89 58.5 90 50.0 91 33.0 92 16.0 93 13.0 94 10.0 95 7.0 96 4.0 97 4.5 98 5.0 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014888179652628415 2 6.143180924628229E-4 3 2.529545086611624E-4 4 0.001915226994148801 5 0.039352494276000835 6 0.027608177802446864 7 0.03020999560581882 8 0.023849996530909596 9 0.030318404680959317 10 0.031149540923703138 11 0.03808772173269502 12 0.03841294895811651 13 0.03606408566340572 14 0.041376130345290134 15 0.03606408566340572 16 0.04220726658803395 17 0.045206584333587735 18 0.04835044751266218 19 0.05044635629871181 20 0.046073856934711714 21 0.046904993177455535 22 0.04477294803302574 23 0.043761129998381096 24 0.040978630403108306 25 0.03895499433381901 26 0.042532493813455446 27 0.04206272115451329 28 0.04188203936261246 29 0.03953317606790167 30 0.036569994680728045 31 0.03472704040333958 32 0.03216135895834779 33 0.024717269132033582 34 0.021248178727537637 35 0.022187724045421957 36 0.023813860172529427 37 0.018718633640926016 38 0.020200224334512823 39 0.014454543352066421 40 0.007986135202016697 41 0.007118862600892712 42 0.006396135433289391 43 0.002132045144429797 44 0.003432954046115775 45 0.0021681815028099633 46 6.143180924628229E-4 47 7.22727167603321E-5 48 3.252272254214945E-4 49 7.22727167603321E-5 50 3.613635838016605E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2767296.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.13040681589125 #Duplication Level Percentage of deduplicated Percentage of total 1 78.66099880335398 49.65900855000071 2 12.30864408534374 15.54099416919528 3 3.931989695789921 7.446843272733307 4 1.7358824284588137 4.383478555726486 5 0.9604411345510618 3.0316519773462334 6 0.5719094133943773 2.166292435765486 7 0.3642147730736992 1.6095118754750104 8 0.2611426923970576 1.3188835526418718 9 0.1914455388264774 1.087743127428272 >10 0.9585552924942903 10.47827805117773 >50 0.042506390183152165 1.8018998178799606 >100 0.012097180588368658 1.225676088003536 >500 0.0 0.0 >1k 1.725715451367886E-4 0.24973852662619647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3034 0.10963771132542381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 3.613635838016605E-5 0.0 0.0 0.0 3.613635838016605E-5 13 3.613635838016605E-5 0.0 0.0 0.0 3.613635838016605E-5 14 3.613635838016605E-5 0.0 0.0 0.0 3.613635838016605E-5 15 3.613635838016605E-5 0.0 0.0 0.0 1.0840907514049816E-4 16 3.613635838016605E-5 0.0 0.0 0.0 1.0840907514049816E-4 17 3.613635838016605E-5 0.0 0.0 0.0 1.0840907514049816E-4 18 3.613635838016605E-5 0.0 0.0 0.0 1.0840907514049816E-4 19 1.0840907514049816E-4 0.0 0.0 0.0 1.0840907514049816E-4 20 1.0840907514049816E-4 0.0 0.0 0.0 1.445454335206642E-4 21 1.0840907514049816E-4 3.613635838016605E-5 0.0 3.613635838016605E-5 1.445454335206642E-4 22 1.445454335206642E-4 3.613635838016605E-5 0.0 1.0840907514049816E-4 1.445454335206642E-4 23 1.8068179190083026E-4 3.613635838016605E-5 0.0 1.0840907514049816E-4 1.445454335206642E-4 24 2.890908670413284E-4 3.613635838016605E-5 0.0 1.8068179190083026E-4 1.8068179190083026E-4 25 2.890908670413284E-4 3.613635838016605E-5 0.0 2.1681815028099633E-4 1.8068179190083026E-4 26 2.890908670413284E-4 3.613635838016605E-5 0.0 2.890908670413284E-4 1.8068179190083026E-4 27 2.890908670413284E-4 3.613635838016605E-5 0.0 3.974999421818266E-4 1.8068179190083026E-4 28 3.252272254214945E-4 3.613635838016605E-5 0.0 7.949998843636532E-4 1.8068179190083026E-4 29 3.252272254214945E-4 3.613635838016605E-5 0.0 0.001300908901685978 1.8068179190083026E-4 30 3.252272254214945E-4 3.613635838016605E-5 0.0 0.0021681815028099633 1.8068179190083026E-4 31 3.252272254214945E-4 3.613635838016605E-5 0.0 0.004083408496958764 1.8068179190083026E-4 32 3.252272254214945E-4 3.613635838016605E-5 0.0 0.00668522630033072 1.8068179190083026E-4 33 3.613635838016605E-4 3.613635838016605E-5 0.0 0.00943158953722334 2.1681815028099633E-4 34 3.613635838016605E-4 7.22727167603321E-5 0.0 0.013804088901223432 2.1681815028099633E-4 35 4.3363630056199266E-4 7.22727167603321E-5 0.0 0.019079997224727675 2.1681815028099633E-4 36 4.697726589421587E-4 7.22727167603321E-5 0.0 0.025584541733157565 2.1681815028099633E-4 37 4.697726589421587E-4 7.22727167603321E-5 0.0 0.0337513587270751 2.1681815028099633E-4 38 5.420453757024908E-4 7.22727167603321E-5 0.0 0.042677039246976106 2.1681815028099633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2235 0.0 25.693426 1 GTATCAA 4825 0.0 23.803032 1 TATACCG 120 3.1610398E-7 18.335865 5 TCAACGC 6840 0.0 16.598482 4 TATCAAC 6780 0.0 16.58221 2 ATCAACG 6860 0.0 16.549791 3 CAACGCA 6940 0.0 16.296198 5 GTGCCGA 220 1.8189894E-11 16.00134 11 AACGCAG 7160 0.0 15.85579 6 GTACATG 6925 0.0 13.534708 1 ACGCAGA 8540 0.0 13.216085 7 TACATGG 7090 0.0 13.09536 2 GTAATAC 320 3.6379788E-12 13.06383 3 ACCGTGC 455 0.0 13.055567 8 CGCAGAG 8645 0.0 13.030119 8 CGTGCCG 220 7.386734E-8 13.001089 10 GTATTAT 560 0.0 12.965373 1 GAGTACT 5300 0.0 12.952029 12 TAGACGG 120 0.001960715 12.835106 5 TATACTG 635 0.0 12.820668 5 >>END_MODULE