FastQCFastQC Report
Mon 6 Feb 2017
SRR2077435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702514
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.025.906791
GTATCAA57750.025.5639081
ATCAACG74450.019.5618043
TATCAAC75050.019.4653572
TCAACGC74750.019.3957884
CAACGCA74950.019.3138945
AACGCAG77550.018.6929636
ACGCAGA91550.015.7155347
TCGATCG1401.5937076E-615.709146541
GTACATG105800.015.5758891
GAGTACT44650.015.32441512
TACATGG106350.015.2466692
CGCAGAG93750.015.2059478
GTATAGA6800.014.8835181
AGAGTAC90650.014.44070111
AGTACTT48400.014.36437313
ACATGGG112850.014.3090283
GTACTTT47050.014.30891814
CAGAGTA97400.013.84708110
GCAGAGT100650.013.6619029
Produced by FastQC (version 0.11.5)