FastQCFastQC Report
Mon 6 Feb 2017
SRR2077435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702514
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.12829120970238062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27050.028.3881341
GTATCAA64150.025.14121
TATCAAC91600.017.5578462
TCAACGC95450.017.0112294
ATCAACG95100.016.9810623
CAACGCA96500.016.7581845
CGTGCCG2251.8189894E-1216.62324110
AACGCAG99100.016.3176336
GTACATG100100.014.4194421
GTGCCGA3450.014.03041611
TACATGG100500.013.9889172
TATACTG10400.013.9629615
CAACGTA950.00624106413.8954029
ACGCAGA118550.013.6404687
ATACCGT2104.020694E-813.61976
GTTACGG2754.3655746E-1113.60120225
CGCAGAG120900.013.3389348
ACATGGG105450.013.2696633
AGAGTAC121700.012.51072511
TAGGACC5500.012.40092854
Produced by FastQC (version 0.11.5)