##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3702514
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.584085569966785	31.0	31.0	34.0	27.0	34.0
2	30.53587292310036	31.0	31.0	34.0	27.0	34.0
3	30.71927614588358	31.0	31.0	34.0	27.0	34.0
4	34.19101507786331	37.0	35.0	37.0	30.0	37.0
5	33.827814560593154	37.0	35.0	37.0	28.0	37.0
6	33.778459176656725	37.0	35.0	37.0	29.0	37.0
7	33.594956021773314	37.0	35.0	37.0	28.0	37.0
8	33.569758007667225	37.0	35.0	37.0	28.0	37.0
9	35.035591222612524	39.0	35.0	39.0	28.0	39.0
10	34.900704764384415	39.0	35.0	39.0	27.0	39.0
11	34.84111714364888	39.0	35.0	39.0	27.0	39.0
12	34.77008756752844	39.0	35.0	39.0	27.0	39.0
13	34.73640828907062	39.0	35.0	39.0	27.0	39.0
14	35.898945689334326	40.0	36.0	41.0	27.0	41.0
15	35.903233586692714	40.0	36.0	41.0	27.0	41.0
16	35.84936343252179	40.0	36.0	41.0	27.0	41.0
17	35.81933032528709	40.0	36.0	41.0	27.0	41.0
18	35.81529793000107	40.0	36.0	41.0	27.0	41.0
19	35.857054423021765	40.0	36.0	41.0	27.0	41.0
20	35.85432870746741	40.0	36.0	41.0	27.0	41.0
21	35.79624141866851	40.0	36.0	41.0	27.0	41.0
22	35.79699252994047	40.0	36.0	41.0	27.0	41.0
23	35.73006962296429	40.0	36.0	41.0	27.0	41.0
24	35.715482237204235	40.0	36.0	41.0	27.0	41.0
25	35.69544342033548	40.0	36.0	41.0	27.0	41.0
26	35.615436970663715	40.0	36.0	41.0	26.0	41.0
27	35.55858829973364	40.0	35.0	41.0	26.0	41.0
28	35.49571048212107	40.0	35.0	41.0	25.0	41.0
29	35.416745757072086	40.0	35.0	41.0	25.0	41.0
30	35.28815772202347	39.0	35.0	41.0	25.0	41.0
31	35.20954275932515	39.0	35.0	41.0	25.0	41.0
32	35.125473934737315	39.0	35.0	41.0	24.0	41.0
33	35.02616519478387	39.0	35.0	41.0	24.0	41.0
34	35.001429839292975	39.0	35.0	41.0	24.0	41.0
35	34.93462360979594	39.0	35.0	41.0	24.0	41.0
36	34.82829882614894	39.0	35.0	41.0	24.0	41.0
37	34.739272289044685	39.0	34.0	41.0	23.0	41.0
38	34.65573742597597	39.0	34.0	41.0	23.0	41.0
39	34.57034058480265	39.0	34.0	41.0	22.0	41.0
40	34.47635471466145	39.0	34.0	41.0	21.0	41.0
41	34.41439222106925	39.0	34.0	41.0	20.0	41.0
42	34.30136118323928	39.0	34.0	41.0	19.0	41.0
43	34.39406171050265	39.0	34.0	41.0	19.0	41.0
44	34.32959551267058	39.0	34.0	41.0	18.0	41.0
45	34.25836985356436	39.0	34.0	41.0	16.0	41.0
46	34.10907831813735	39.0	34.0	41.0	14.0	41.0
47	33.95994478346334	39.0	34.0	41.0	9.0	41.0
48	33.80715400400917	39.0	34.0	41.0	2.0	41.0
49	33.65555997897645	39.0	33.0	41.0	2.0	41.0
50	33.51358428354356	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7473058414425857
1101	2	0.980876456202413
1101	3	0.7994479969520292
1101	4	0.8032968494552648
1101	5	0.8847938798436132
1101	6	0.9595971889450183
1101	7	0.9502520749258494
1101	8	0.928231154062054
1101	9	0.992837314791835
1101	10	1.0720872676415567
1101	11	1.106660878181259
1101	12	1.1354052363627218
1101	13	1.128832304250409
1101	14	1.281206674591047
1101	15	1.250623823076424
1101	16	1.238041950082824
1101	17	1.241915993677253
1101	18	1.2621372305715468
1101	19	1.2117410996516753
1101	20	1.177742274090285
1101	21	1.2684254729799633
1101	22	1.2611681421913161
1101	23	1.3182997243330021
1101	24	1.2883756519875362
1101	25	1.2386029684145967
1101	26	1.1986218121890886
1101	27	1.1880733231227154
1101	28	1.209963654470286
1101	29	1.2802618907023273
1101	30	1.3874483641261577
1101	31	1.3935329095098226
1101	32	1.4444423741921213
1101	33	1.4075909313252737
1101	34	1.3611506416175914
1101	35	1.3513910015415007
1101	36	1.3211862008324005
1101	37	1.474043366069992
1101	38	1.4059096701884002
1101	39	1.4090446845799889
1101	40	1.4549518832656645
1101	41	1.469841896924585
1101	42	1.4258712792874988
1101	43	1.4054336380900452
1101	44	1.4000067497968018
1101	45	1.430949029393652
1101	46	1.4119018280972924
1101	47	1.4024619641559397
1101	48	1.5221613302993262
1101	49	1.451280637505782
1101	50	1.4574211495195044
1102	1	0.5531874030941317
1102	2	0.7077320195724823
1102	3	0.5447436881662142
1102	4	0.5857526211034312
1102	5	0.6038412816608911
1102	6	0.5812907930087334
1102	7	0.585605976969596
1102	8	0.6196599633971118
1102	9	0.7684403000196554
1102	10	0.7184128004850976
1102	11	0.7812651302120486
1102	12	0.7774062986387378
1102	13	0.7285860474817625
1102	14	0.7840250518424838
1102	15	0.8083440490330176
1102	16	0.7970517538420694
1102	17	0.7288950055846755
1102	18	0.7902663503519989
1102	19	0.8422915725678664
1102	20	0.7871083030638601
1102	21	0.8912843722622199
1102	22	0.8637207855527151
1102	23	0.847741712018923
1102	24	0.9052760979277323
1102	25	0.8884425930413471
1102	26	0.8838861297797962
1102	27	0.8223769377123205
1102	28	0.9177312610151205
1102	29	0.8898623321562695
1102	30	0.9521101056116521
1102	31	0.9550957619744409
1102	32	1.014660179249013
1102	33	1.025821420763009
1102	34	0.9653015425220559
1102	35	0.9297965830867838
1102	36	0.9267453628806095
1102	37	0.9646995004804069
1102	38	0.8957609563551259
1102	39	0.8963012197038367
1102	40	0.9150023836284475
1102	41	0.9220360410232686
1102	42	0.9448897780184637
1102	43	0.9736360935264301
1102	44	0.9692945995974824
1102	45	0.8769352618512727
1102	46	0.9817176212465526
1102	47	0.9460813038451121
1102	48	1.0287856126429773
1102	49	1.0501056621235634
1102	50	1.0064089953148425
1103	1	0.4335236087445402
1103	2	0.607409118130704
1103	3	0.467350536755216
1103	4	0.5438707983465463
1103	5	0.5212702634204689
1103	6	0.5003944453753846
1103	7	0.49628253747028594
1103	8	0.44475860727797567
1103	9	0.5862635896123933
1103	10	0.5153477463848901
1103	11	0.584931349835891
1103	12	0.5918902854041335
1103	13	0.7218970688273885
1103	14	0.8243372724407223
1103	15	0.8005999020817427
1103	16	0.8058910887922508
1103	17	0.8682728531403967
1103	18	0.8196853151400134
1103	19	0.764935563187997
1103	20	0.7347506875421033
1103	21	0.8517617711689738
1103	22	0.8469028738583972
1103	23	0.8456452283988938
1103	24	0.8501047895110148
1103	25	0.8503349360275934
1103	26	0.8115331185314503
1103	27	0.8515482889594921
1103	28	0.8308121489974312
1103	29	0.8103533565000234
1103	30	0.8334298054772731
1103	31	0.7202371961520697
1103	32	0.8112156072055186
1103	33	0.7982944241948005
1103	34	0.9166580631904182
1103	35	0.8744900910612756
1103	36	0.841633294330471
1103	37	0.890313247448816
1103	38	0.7125432652376276
1103	39	0.6760784879777191
1103	40	0.7153906684295279
1103	41	0.7365605159487103
1103	42	0.7181997152183115
1103	43	0.7355329203254399
1103	44	0.774148895759339
1103	45	0.6861753961247388
1103	46	0.8256039760004654
1103	47	0.829543730832583
1103	48	0.8874204991155139
1103	49	0.9514301268767795
1103	50	0.9028651770872784
1104	1	0.48074237042387225
1104	2	0.6945592572445278
1104	3	0.539111468610205
1104	4	0.578119791328561
1104	5	0.6714187207104487
1104	6	0.6708270478420602
1104	7	0.6509018431469613
1104	8	0.6951224833628586
1104	9	0.7579611935324948
1104	10	0.8235476757898113
1104	11	0.8280639024707455
1104	12	0.818182798173531
1104	13	0.8598050668341202
1104	14	0.9181498217534525
1104	15	0.9083279864636182
1104	16	0.9750955445872762
1104	17	0.9237647347067792
1104	18	0.974037293878439
1104	19	0.8723963206316228
1104	20	0.891104244288087
1104	21	0.9115308837473606
1104	22	0.9098871768358521
1104	23	0.9369329656191141
1104	24	0.9779960730955821
1104	25	0.8546292967268982
1104	26	0.9190167155581506
1104	27	0.9580279824938174
1104	28	0.8976656849801614
1104	29	0.9554148157417472
1104	30	0.9757328659813993
1104	31	0.8241230397632151
1104	32	0.7920085560033243
1104	33	0.8697060142450468
1104	34	0.8942747526966741
1104	35	1.0402509606264942
1104	36	1.0641339172281903
1104	37	0.986929355583861
1104	38	1.1235893181424075
1104	39	1.1341590286529382
1104	40	1.0308089442486335
1104	41	0.9531488013189247
1104	42	1.1014017209489708
1104	43	1.091493013344838
1104	44	1.117780285651783
1104	45	1.0128136171288489
1104	46	0.9740456935371071
1104	47	1.0766280504206378
1104	48	1.037116317395487
1104	49	1.1148270995938532
1104	50	1.0405572372176692
1105	1	0.44320095264074055
1105	2	0.5941272995708289
1105	3	0.48373753694235333
1105	4	0.48044550776371153
1105	5	0.5468038649841063
1105	6	0.612501456790767
1105	7	0.6109426751202562
1105	8	0.563553962111321
1105	9	0.6357461026600291
1105	10	0.5441300015987451
1105	11	0.5684671554624927
1105	12	0.5801731436163422
1105	13	0.5457750240391661
1105	14	0.48267086568714035
1105	15	0.592629927241326
1105	16	0.7006826904806474
1105	17	0.7028555886736356
1105	18	0.6455755174563578
1105	19	0.5867035439752186
1105	20	0.6925956441886001
1105	21	0.712240946932603
1105	22	0.7983001666037026
1105	23	0.6805684500735651
1105	24	0.642048263613276
1105	25	0.6077967807867068
1105	26	0.6366389031570705
1105	27	0.5517159328993557
1105	28	0.6312329482821539
1105	29	0.6111102234037276
1105	30	0.6623022505316456
1105	31	0.6511619149261136
1105	32	0.6896955835014467
1105	33	0.6416484466097003
1105	34	0.6206591652219018
1105	35	0.699684246135817
1105	36	0.7293687755855416
1105	37	0.6718217310119101
1105	38	0.7007733610935745
1105	39	0.6976094280313632
1105	40	0.7896389997875204
1105	41	0.7408423241120659
1105	42	0.7849500738983721
1105	43	0.7487988841871243
1105	44	0.7526026208336773
1105	45	0.7625212260792011
1105	46	0.742486370527736
1105	47	0.6717476378460745
1105	48	0.6230129969842224
1105	49	0.6887835358968033
1105	50	0.7765001135645164
1106	1	0.327953310705027
1106	2	0.4677015229708559
1106	3	0.41977659349226926
1106	4	0.4144151580085875
1106	5	0.3248237266975238
1106	6	0.2792157916527245
1106	7	0.3246183261354645
1106	8	0.3182775385160923
1106	9	0.3099073335527578
1106	10	0.3556857007786718
1106	11	0.40316077226628977
1106	12	0.326289956767404
1106	13	0.31745905651295914
1106	14	0.25304214784836887
1106	15	0.37322452591231325
1106	16	0.41142392728178123
1106	17	0.4398808751502443
1106	18	0.4423472411274929
1106	19	0.38242933042253213
1106	20	0.30122926949352546
1106	21	0.34730754736862224
1106	22	0.3208725788408344
1106	23	0.4652027335299067
1106	24	0.48346724056177237
1106	25	0.4291474682931735
1106	26	0.38828506076190195
1106	27	0.3898028313234079
1106	28	0.41842043542763463
1106	29	0.3605419133944707
1106	30	0.45959120891977534
1106	31	0.4988133240307917
1106	32	0.43625290936243744
1106	33	0.4649922546887595
1106	34	0.39271889246301583
1106	35	0.3950728546317066
1106	36	0.47054624337289397
1106	37	0.4236986778873728
1106	38	0.3654078190597616
1106	39	0.41426975832864343
1106	40	0.4871855160100793
1106	41	0.3802638489043062
1106	42	0.41572360748590853
1106	43	0.4915903499077814
1106	44	0.42923646926190173
1106	45	0.45753111704794236
1106	46	0.4575141612206224
1106	47	0.4758863881312152
1106	48	0.46322100025503943
1106	49	0.5802665828885623
1106	50	0.5499058726387176
1107	1	0.26557304558812334
1107	2	0.49391082420437726
1107	3	0.3165898383043739
1107	4	0.3075458470824515
1107	5	0.37266880654002676
1107	6	0.3449945510860388
1107	7	0.37621753935888336
1107	8	0.28013796150501236
1107	9	0.23643961560149762
1107	10	0.29106522261266576
1107	11	0.36326646402621776
1107	12	0.36676299532344103
1107	13	0.461239210114627
1107	14	0.3945289902515725
1107	15	0.4318806868249325
1107	16	0.46796260572750725
1107	17	0.5035703576590862
1107	18	0.44438627692953503
1107	19	0.3638483214756363
1107	20	0.38617521469605265
1107	21	0.33364937817883344
1107	22	0.3557027156726704
1107	23	0.4491547524981385
1107	24	0.4382589534208421
1107	25	0.47602094622347835
1107	26	0.5208761851038162
1107	27	0.43988617900519955
1107	28	0.42748134435427687
1107	29	0.43460058001088697
1107	30	0.46192868245997687
1107	31	0.530182902195321
1107	32	0.4716070978778859
1107	33	0.41750898387315516
1107	34	0.412083668998676
1107	35	0.3530024036910362
1107	36	0.3899714885795049
1107	37	0.3794283770014033
1107	38	0.4163935921294595
1107	39	0.5056098817613233
1107	40	0.46225376172107957
1107	41	0.4585096100565451
1107	42	0.43591005280796224
1107	43	0.4467866056220302
1107	44	0.42411834867500176
1107	45	0.3938884715443507
1107	46	0.4773307735252601
1107	47	0.550052770741523
1107	48	0.47795800104847075
1107	49	0.45187702058052537
1107	50	0.5471576254931207
1108	1	0.22669368056367745
1108	2	0.38615355347451796
1108	3	0.2668934196831927
1108	4	0.20962029212398647
1108	5	0.1998944946068164
1108	6	0.2715007976152748
1108	7	0.18766348226738216
1108	8	0.23808177824090393
1108	9	0.2085810840572151
1108	10	0.32849512350741605
1108	11	0.3347403313995301
1108	12	0.3448733013529832
1108	13	0.32072640546728337
1108	14	0.2378639372872584
1108	15	0.34837953790485443
1108	16	0.2238262505938735
1108	17	0.23953789761972644
1108	18	0.2801581497588117
1108	19	0.3649267469524702
1108	20	0.3371340098788451
1108	21	0.38837246895980115
1108	22	0.3445890386253083
1108	23	0.4079514608138268
1108	24	0.35525280746959
1108	25	0.25148474377677843
1108	26	0.261578716480777
1108	27	0.3035670172981
1108	28	0.24690936268781627
1108	29	0.4464648468040622
1108	30	0.5487242677437862
1108	31	0.5506897060300489
1108	32	0.5021531806741493
1108	33	0.40870611395431666
1108	34	0.464251136094056
1108	35	0.43132020883859923
1108	36	0.5877889416507145
1108	37	0.542135553851864
1108	38	0.4721338127163719
1108	39	0.4917354586812692
1108	40	0.5324829791553753
1108	41	0.511109655559963
1108	42	0.4328953370567916
1108	43	0.4909240957476726
1108	44	0.4981738011507062
1108	45	0.5160171209975175
1108	46	0.6160534333753844
1108	47	0.5042916623351701
1108	48	0.4992429761553794
1108	49	0.5174351966861934
1108	50	0.5254690569892944
1109	1	0.12687308910056316
1109	2	0.28286724515143646
1109	3	0.26078188270470903
1109	4	0.2291399844763049
1109	5	0.15227980090342186
1109	6	0.13462811182348844
1109	7	0.1531396523345876
1109	8	0.17754629559710366
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2211	5	-0.14994962904079756
2211	6	-0.21144041583060869
2211	7	-0.1902214989048332
2211	8	-0.12890321867676136
2211	9	-0.2441316188908047
2211	10	-0.32003572761556853
2211	11	-0.35775001158679487
2211	12	-0.13796447487411712
2211	13	-0.19595691282344774
2211	14	-0.18904162161453542
2211	15	-0.2665675468851063
2211	16	-0.2602221831323561
2211	17	-0.20093013722959796
2211	18	-0.2939247798378446
2211	19	-0.23223116958854462
2211	20	-0.20994482139374782
2211	21	-0.2155465285585052
2211	22	-0.23363217539345982
2211	23	-0.10036935617399934
2211	24	-0.1723656814116552
2211	25	-0.26113632086803307
2211	26	-0.21137992127403749
2211	27	-0.19257481864534753
2211	28	-0.18920087097509253
2211	29	-0.1679547081701216
2211	30	-0.26244392833498864
2211	31	-0.28295875718904995
2211	32	-0.13588171020268192
2211	33	-0.10441516247157523
2211	34	-0.09415292133252251
2211	35	-0.1516733509126169
2211	36	-0.18198253016984722
2211	37	-0.05134910371214829
2211	38	-0.0663225096802833
2211	39	-0.1781996472830869
2211	40	-0.10897798226793043
2211	41	-0.09823219286666784
2211	42	-0.1349764969796894
2211	43	-0.10571247952911733
2211	44	-0.21299199907453925
2211	45	-0.2502761196277774
2211	46	-0.11069089607693172
2211	47	-0.10417919166460621
2211	48	-0.08544580468139884
2211	49	-0.12578923670550068
2211	50	-0.13605315518095296
2212	1	-0.9327649534059219
2212	2	-2.41760967434028
2212	3	-1.2651544244571227
2212	4	-0.7761687410124054
2212	5	-0.6708511810440143
2212	6	-0.7096039008871671
2212	7	-0.6725234640932456
2212	8	-0.665933557013318
2212	9	-0.6398977850944476
2212	10	-0.6614412968528285
2212	11	-0.699635521292862
2212	12	-0.6352152230449661
2212	13	-2.1888138879348915
2212	14	-1.5075475372291365
2212	15	-1.0824553026810193
2212	16	-0.8608048941291528
2212	17	-0.777180120580212
2212	18	-0.885303710056931
2212	19	-0.7319306043506444
2212	20	-0.8500612374082834
2212	21	-0.7387081909454025
2212	22	-0.8630676595470987
2212	23	-0.7564948581968096
2212	24	-0.8101912294919273
2212	25	-0.9008249352815625
2212	26	-0.8922787462830328
2212	27	-0.9162977931861533
2212	28	-0.8717458821529647
2212	29	-0.7736550023910738
2212	30	-0.8224642651222851
2212	31	-0.7364266140590061
2212	32	-0.880981007142907
2212	33	-0.8104072415698269
2212	34	-0.8256400305013187
2212	35	-0.7496867987096039
2212	36	-0.7561875307341737
2212	37	-0.8422541047671928
2212	38	-0.8279071527069277
2212	39	-0.840423556643124
2212	40	-0.7070730996864185
2212	41	-0.7756681207702698
2212	42	-0.74846695144835
2212	43	-0.8338939844794169
2212	44	-0.8890028271343979
2212	45	-0.8523732237561958
2212	46	-0.7719139144319129
2212	47	-0.8133564736295043
2212	48	-0.7405457045046688
2212	49	-0.7692543700863581
2212	50	-0.7112472169231907
2213	1	-0.8461140161250569
2213	2	-2.0066362251997027
2213	3	-1.0722105151890347
2213	4	-0.6991750628502515
2213	5	-0.6749225742621903
2213	6	-0.6413878968817599
2213	7	-0.6467251404618537
2213	8	-0.6114576657351805
2213	9	-0.6814589803558633
2213	10	-0.6483820924761048
2213	11	-0.660178471612781
2213	12	-0.7074377079011356
2213	13	-0.7389868502953902
2213	14	-0.7733483714022995
2213	15	-0.6931205926682935
2213	16	-0.8991632684154709
2213	17	-0.7980118870632964
2213	18	-0.8755635421765362
2213	19	-0.8448747213383072
2213	20	-0.909479511573771
2213	21	-0.8289251898559513
2213	22	-0.8384790338412955
2213	23	-0.9651880298813396
2213	24	-0.840120576185214
2213	25	-0.8416924546012723
2213	26	-0.9283079752924266
2213	27	-0.9356462449232268
2213	28	-0.9732248634388654
2213	29	-0.9692342642416492
2213	30	-0.9487228487756099
2213	31	-0.9409139120069483
2213	32	-0.8919224567247142
2213	33	-0.9454234436967326
2213	34	-0.9906245267419536
2213	35	-0.9386891394943433
2213	36	-0.94812355238858
2213	37	-0.8978802431958144
2213	38	-1.061915789543633
2213	39	-0.9658772269405915
2213	40	-0.998135588897533
2213	41	-0.9888594061461689
2213	42	-0.9900084670706519
2213	43	-1.0635377346629653
2213	44	-0.9786246915536836
2213	45	-0.9673187009489439
2213	46	-0.9138463726211867
2213	47	-0.9014671781355119
2213	48	-1.0581412358119522
2213	49	-1.0967568716746996
2213	50	-1.0700299587213067
2214	1	-0.8264128563223174
2214	2	-1.790279346700018
2214	3	-0.9325527956926116
2214	4	-0.4877619977808507
2214	5	-0.5184457110770069
2214	6	-0.4548340885246702
2214	7	-0.3817230116899637
2214	8	-0.45667590967942573
2214	9	-0.5589690766410413
2214	10	-0.6030869771901877
2214	11	-0.5849212874910634
2214	12	-0.5489318315414735
2214	13	-0.4650908145221777
2214	14	-0.643564303756925
2214	15	-0.6932849528431362
2214	16	-0.7458219930578593
2214	17	-0.5550384163775917
2214	18	-0.7271065765923268
2214	19	-0.7734145209349137
2214	20	-0.7405094293444634
2214	21	-0.8085528780237397
2214	22	-0.7005926248281042
2214	23	-0.7501327609160171
2214	24	-0.6577079410890576
2214	25	-0.6303474188234901
2214	26	-0.6058917611875501
2214	27	-0.5848359756291899
2214	28	-0.6671316362382598
2214	29	-0.7772001427177813
2214	30	-0.598430270085764
2214	31	-0.6502316045799432
2214	32	-0.5479202143948783
2214	33	-0.5983886243013217
2214	34	-0.6905521555291401
2214	35	-0.667318625252662
2214	36	-0.7564632403960587
2214	37	-0.702896779411482
2214	38	-0.812181490510774
2214	39	-0.9230843415036745
2214	40	-0.86160466791452
2214	41	-0.7782488054598318
2214	42	-0.8550521517199101
2214	43	-0.8951490655655263
2214	44	-0.8606853673535042
2214	45	-0.9566756325360828
2214	46	-0.8458407242563339
2214	47	-0.8680244529981067
2214	48	-0.8803914197722946
2214	49	-0.8667872791859779
2214	50	-0.95244563492016
2215	1	-0.5281003367454034
2215	2	-0.2934966789707474
2215	3	-0.5371432984167477
2215	4	-0.5076514815582769
2215	5	-0.665164411033345
2215	6	-0.6359212587128695
2215	7	-0.7583464912989086
2215	8	-0.7552522082098037
2215	9	-0.9137337065156643
2215	10	-0.9259025240023107
2215	11	-0.8445381504885745
2215	12	-0.8575500131033351
2215	13	-0.8279325872400989
2215	14	-0.9525148798413525
2215	15	-1.0143525097720314
2215	16	-1.0351834299185398
2215	17	-1.013494938336997
2215	18	-1.0206158961946201
2215	19	-1.0199362732818287
2215	20	-0.9939400976623034
2215	21	-1.047189126516507
2215	22	-1.0376850560572422
2215	23	-1.0339594655227984
2215	24	-1.0337233523881864
2215	25	-1.0454335043790692
2215	26	-1.1006856787863057
2215	27	-1.067002976452578
2215	28	-1.183677900753807
2215	29	-1.0627431176509603
2215	30	-0.8731541227078878
2215	31	-0.9096919624029809
2215	32	-1.019308403177348
2215	33	-1.0201297592358713
2215	34	-0.9612837494853324
2215	35	-1.105758124383243
2215	36	-0.9859705343522052
2215	37	-0.9272646240631914
2215	38	-0.9953953027917919
2215	39	-0.9553053536835421
2215	40	-0.8817702660286173
2215	41	-1.051796611457732
2215	42	-1.0440001549958353
2215	43	-0.9063933180479111
2215	44	-0.8676637799923341
2215	45	-0.912040048391809
2215	46	-0.8197291410231173
2215	47	-0.8101409976986744
2215	48	-0.8129147810056239
2215	49	-0.799616271565796
2215	50	-0.8067443838039239
2216	1	-1.4113341690017371
2216	2	-1.0207829380257643
2216	3	-1.1880899163146275
2216	4	-2.509068567884917
2216	5	-1.839690678915666
2216	6	-1.6013664100136822
2216	7	-1.514798016155737
2216	8	-1.4875001736673852
2216	9	-1.6414266307322762
2216	10	-1.6200836575740425
2216	11	-1.8475697842563363
2216	12	-1.7279533161892715
2216	13	-1.8029896991515173
2216	14	-1.9229640015228853
2216	15	-2.025222551054263
2216	16	-1.9139788568185736
2216	17	-1.9541255274704028
2216	18	-1.9884142888727823
2216	19	-1.9903236123269537
2216	20	-1.991595160971528
2216	21	-1.9950476493571472
2216	22	-2.0229657132044423
2216	23	-1.9869198165158792
2216	24	-2.0300993352769297
2216	25	-2.1089352638089096
2216	26	-2.2473862215213813
2216	27	-2.4111888071973553
2216	28	-2.2676033705821936
2216	29	-2.2335458179753402
2216	30	-2.0928803663849536
2216	31	-2.028681458720037
2216	32	-2.079172075135247
2216	33	-2.0822635728093104
2216	34	-2.119291361021503
2216	35	-2.2448810902375413
2216	36	-2.272886453050482
2216	37	-2.282510181479047
2216	38	-2.4107280710537964
2216	39	-2.5192010303166086
2216	40	-2.451625322270921
2216	41	-2.4558114143235166
2216	42	-2.601770940145233
2216	43	-2.313374216422453
2216	44	-2.1807430583288294
2216	45	-2.1462851587298886
2216	46	-2.2024677600584717
2216	47	-2.259301822344529
2216	48	-2.2249463041147592
2216	49	-2.1743316641383785
2216	50	-2.1368884177488248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88366.0
3	14064.0
4	24365.0
5	14472.0
6	10784.0
7	8476.0
8	7210.0
9	6288.0
10	6192.0
11	5993.0
12	6187.0
13	6596.0
14	7145.0
15	7787.0
16	8290.0
17	9086.0
18	9758.0
19	10965.0
20	13154.0
21	15968.0
22	19969.0
23	24539.0
24	29195.0
25	32600.0
26	35755.0
27	40637.0
28	47240.0
29	54865.0
30	64141.0
31	76326.0
32	93375.0
33	115445.0
34	145377.0
35	186293.0
36	242412.0
37	328367.0
38	519760.0
39	1365072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39724676023497	18.474859034361742	14.065850415698037	26.062043789705253
2	14.535302199620096	20.38188760414239	38.51707885179296	26.565731344444554
3	17.340711049098974	25.020898036219304	29.94116679509337	27.697224119588356
4	11.981611005955005	16.682845221735334	37.27052920890917	34.06501456340049
5	13.382459786531484	38.008911498621615	34.654440983274796	13.954187731572109
6	31.925932189607153	37.42649992827285	17.35659364365031	13.29097423846969
7	27.751831131958483	32.18115636817323	22.016810400607987	18.050202099260307
8	26.500604188554377	34.78548402216411	19.94505706765534	18.76885472162617
9	26.546628846630277	15.075313618752311	19.16472437779439	39.21333315682302
10	14.052826159550522	29.013753908035667	34.177980562757135	22.755439369656678
11	34.85140168356036	22.818957143648415	23.858084858899485	18.47155631389174
12	23.23311033536157	24.84345110075978	30.739108584521276	21.18432997935738
13	27.97195164027463	21.806105567982527	26.286839950188845	23.935102841554002
14	22.055332401327433	20.690476949606293	27.72264520351107	29.531545445555203
15	23.34874569761055	28.936779225649605	24.10583655148507	23.60863852525478
16	23.259327830408534	27.96883279144412	25.551609712232647	23.220229665914697
17	22.079337325355773	27.717021973957014	27.0734995261939	23.130141174493314
18	22.165729840677432	27.23742526603751	28.092674166678826	22.504170726606237
19	23.09184320611252	26.747688497218526	28.20645434645209	21.954013950216865
20	23.338877972542686	26.22492753059279	28.04087793363294	22.395316563231585
21	23.479124131140384	26.153093307767882	27.929020208450652	22.438762352641085
22	23.164563426452702	26.027236246976056	27.895496121594967	22.91270420497628
23	22.908139498437684	26.0626558397975	27.80192795513327	23.227276706631546
24	22.682221021345583	26.278762496622534	27.89113753039719	23.14787895163469
25	22.882540200623495	26.052576260543596	28.021551651931258	23.04333188690165
26	22.78365373703443	26.30612198577881	27.919678050752296	22.990546226434468
27	22.890612102188427	26.38848171302257	27.770417839445745	22.950488345343256
28	22.935641247618214	26.200863333300912	27.915479151062794	22.948016268018083
29	22.932353072299655	26.408983384467398	27.733335494879164	22.92532804835378
30	23.0137164697335	26.473405308752284	27.656706485596043	22.856171735918174
31	23.062148342778404	26.12394545049274	27.748353585637187	23.065552621091665
32	22.926550399744094	26.43011989080794	27.62459717596305	23.01873253348492
33	22.93903401683255	26.298654811734696	27.611235890705682	23.151075280727074
34	23.07037939612902	26.274781853236483	27.777692232675832	22.877146517958664
35	23.13818155196629	26.27850805450552	27.63633740774889	22.9469729857793
36	23.15697651487359	26.338223208926152	27.53746830477769	22.967331971422567
37	23.193274496069396	25.969292642676674	27.69599732458023	23.1414355366737
38	23.090365619922228	26.15668884959864	27.68400195040514	23.06894358007399
39	23.15450454806492	26.54055333124078	27.371722527251386	22.93321959344292
40	23.195373388067853	26.242938722944036	27.571704003582703	22.989983885405408
41	23.000880004451343	26.310755555939707	27.503434394290615	23.18493004531834
42	23.23348386248124	26.32312636048179	27.351845744492643	23.09154403254433
43	23.063493216746743	26.117841080999032	27.51938120589548	23.29928449635874
44	23.142311775984865	26.399804232817093	27.255799481249376	23.202084509948662
45	23.182610908075354	26.46357929274773	27.150293605818693	23.203516193358222
46	23.34011887710909	26.23706981187469	26.993265863222994	23.42954544779322
47	23.21706135075869	26.47875204220287	27.012364898607615	23.291821708430824
48	23.14909695907349	26.61068632190769	26.9162914687143	23.323925250304526
49	23.258830761385784	26.424897475011562	26.94060140790901	23.375670355693646
50	23.37980549914477	26.52489410542004	26.860261828282113	23.235038567153087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	671.0
1	492.0
2	313.0
3	539.5
4	766.0
5	873.5
6	981.0
7	939.5
8	898.0
9	1249.5
10	1601.0
11	2345.5
12	3090.0
13	5829.0
14	8568.0
15	11739.5
16	14911.0
17	16978.5
18	19046.0
19	20400.5
20	21755.0
21	24314.5
22	26874.0
23	31435.0
24	35996.0
25	43922.5
26	51849.0
27	60558.5
28	69268.0
29	77492.0
30	85716.0
31	95965.5
32	106215.0
33	118637.5
34	131060.0
35	144173.5
36	157287.0
37	167326.0
38	177365.0
39	187796.0
40	198227.0
41	209035.5
42	219844.0
43	226973.0
44	234102.0
45	242426.0
46	250750.0
47	254673.5
48	258597.0
49	259096.0
50	259595.0
51	257076.0
52	254557.0
53	247519.0
54	240481.0
55	227624.0
56	214767.0
57	199638.0
58	184509.0
59	167529.0
60	150549.0
61	130791.0
62	111033.0
63	95134.5
64	79236.0
65	65968.0
66	52700.0
67	42469.5
68	32239.0
69	26102.5
70	19966.0
71	15620.5
72	11275.0
73	9359.5
74	7444.0
75	5697.0
76	3950.0
77	3028.5
78	2107.0
79	1613.0
80	1119.0
81	864.0
82	609.0
83	479.0
84	349.0
85	251.5
86	154.0
87	110.0
88	66.0
89	48.0
90	30.0
91	24.5
92	19.0
93	11.5
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014800754298295698
2	2.9709543299498664E-4
3	3.511127844486206E-4
4	0.0022147114095989913
5	0.03924360583106506
6	0.02665756294236835
7	0.03011467343540092
8	0.023092417746428506
9	0.03038476019266909
10	0.03149211589746859
11	0.03859539761362145
12	0.03873044099225553
13	0.03605658209530065
14	0.04148532591639086
15	0.0370829117729197
16	0.04248464691828309
17	0.04410516746189211
18	0.04645492225012518
19	0.04815646882091466
20	0.04434824554343346
21	0.04553662727541341
22	0.044213202164799376
23	0.043159863811453515
24	0.04089113505040089
25	0.03921659715533824
26	0.04207951678238084
27	0.041782421349385854
28	0.04078310034749362
29	0.03940565788542596
30	0.03627265150111519
31	0.034814183011867075
32	0.030951942382932245
33	0.023659599936691665
34	0.020607619579561347
35	0.021525914554273124
36	0.023011391719248056
37	0.017960769358333285
38	0.0194192378475814
39	0.013504337863408484
40	0.007562429203508751
41	0.007022255688972412
42	0.006509090850162889
43	0.0023497547882330764
44	0.0042403620891102636
45	0.0027278762484085135
46	9.453036504385939E-4
47	4.591474873558885E-4
48	6.482082174436072E-4
49	5.401735145363393E-5
50	2.9709543299498664E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3702514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.490712426504466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77005123053621	40.93552698522187
2	13.629623618282638	15.126350493672302
3	5.1622743875065655	8.593748505115089
4	2.55374227962559	5.668359138004382
5	1.3854931299586208	3.84410004217157
6	0.8397783826605764	2.795994044052784
7	0.553494363654389	2.1499657594265744
8	0.3814171847252353	1.6932089049691967
9	0.28537321813280725	1.425200686347036
>10	1.3518855532961112	13.109633257486788
>50	0.06671109695184474	2.504627032371405
>100	0.019764139444872843	1.7906169715123557
>500	2.4463452247498084E-4	0.07946772119311421
>1k	1.4678070211235933E-4	0.2832004584556514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4750	0.12829120970238062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	2.700867572681697E-5
15	0.0	0.0	0.0	0.0	2.700867572681697E-5
16	0.0	0.0	0.0	0.0	2.700867572681697E-5
17	0.0	0.0	0.0	0.0	2.700867572681697E-5
18	0.0	0.0	0.0	2.700867572681697E-5	2.700867572681697E-5
19	0.0	0.0	0.0	2.700867572681697E-5	2.700867572681697E-5
20	0.0	0.0	0.0	2.700867572681697E-5	2.700867572681697E-5
21	0.0	0.0	0.0	8.10260271804509E-5	2.700867572681697E-5
22	0.0	0.0	0.0	8.10260271804509E-5	2.700867572681697E-5
23	0.0	0.0	0.0	1.0803470290726788E-4	2.700867572681697E-5
24	0.0	0.0	0.0	2.4307808154135272E-4	2.700867572681697E-5
25	0.0	0.0	0.0	2.4307808154135272E-4	2.700867572681697E-5
26	0.0	0.0	0.0	3.241041087218036E-4	2.700867572681697E-5
27	0.0	0.0	0.0	3.241041087218036E-4	2.700867572681697E-5
28	0.0	0.0	0.0	4.591474873558885E-4	2.700867572681697E-5
29	0.0	0.0	0.0	9.453036504385939E-4	2.700867572681697E-5
30	0.0	0.0	0.0	0.00178257259796992	2.700867572681697E-5
31	0.0	0.0	0.0	0.003916257980388461	2.700867572681697E-5
32	0.0	0.0	0.0	0.005806865281265648	2.700867572681697E-5
33	0.0	0.0	0.0	0.008129611393771908	2.700867572681697E-5
34	0.0	0.0	0.0	0.010803470290726787	2.700867572681697E-5
35	0.0	0.0	0.0	0.01488178032547615	2.700867572681697E-5
36	0.0	0.0	0.0	0.019365220496127766	2.700867572681697E-5
37	0.0	0.0	0.0	0.02557721591329567	2.700867572681697E-5
38	0.0	0.0	0.0	0.03189724603337084	2.700867572681697E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2705	0.0	28.388134	1
GTATCAA	6415	0.0	25.1412	1
TATCAAC	9160	0.0	17.557846	2
TCAACGC	9545	0.0	17.011229	4
ATCAACG	9510	0.0	16.981062	3
CAACGCA	9650	0.0	16.758184	5
CGTGCCG	225	1.8189894E-12	16.623241	10
AACGCAG	9910	0.0	16.317633	6
GTACATG	10010	0.0	14.419442	1
GTGCCGA	345	0.0	14.030416	11
TACATGG	10050	0.0	13.988917	2
TATACTG	1040	0.0	13.962961	5
CAACGTA	95	0.006241064	13.895402	9
ACGCAGA	11855	0.0	13.640468	7
ATACCGT	210	4.020694E-8	13.6197	6
GTTACGG	275	4.3655746E-11	13.601202	25
CGCAGAG	12090	0.0	13.338934	8
ACATGGG	10545	0.0	13.269663	3
AGAGTAC	12170	0.0	12.510725	11
TAGGACC	550	0.0	12.4009285	4
>>END_MODULE