FastQCFastQC Report
Mon 6 Feb 2017
SRR2077434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983815
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.11706489846052788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53450.023.2987061
GGTATCA26000.021.7481771
TATCAAC67250.018.1232812
TCAACGC68100.017.7349534
CAACGCA68900.017.5606675
ATCAACG68950.017.5157343
AACGCAG71100.017.0470946
GTATAGA6500.015.570681
TTATGCG1004.947634E-415.39962518
ACGCAGA82000.014.782077
GTACATG78100.014.7055941
GTATTAC3650.014.46711
CGCAGAG83550.014.3235268
AGAGTAC77150.014.23050911
TTAGGCG1402.7564574E-514.1444130
GAGTACT41000.014.00618112
TACATGG79300.013.982242
TAGCCCG950.006245065513.8939315
AGTACTT43750.013.42753513
CAGAGTA84950.013.26057610
Produced by FastQC (version 0.11.5)