##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077434_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2983815
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.875015709754123	31.0	30.0	34.0	26.0	34.0
2	29.90424473367149	31.0	30.0	34.0	25.0	34.0
3	29.978727903707167	31.0	30.0	34.0	25.0	34.0
4	33.37208204932276	37.0	35.0	37.0	27.0	37.0
5	33.456127474391	37.0	35.0	37.0	28.0	37.0
6	33.529359226359546	37.0	35.0	37.0	28.0	37.0
7	33.40148802791058	37.0	35.0	37.0	27.0	37.0
8	33.3378734941677	37.0	35.0	37.0	27.0	37.0
9	34.65395475255671	39.0	35.0	39.0	27.0	39.0
10	34.56963618723011	39.0	35.0	39.0	27.0	39.0
11	34.634033611333145	39.0	35.0	39.0	27.0	39.0
12	34.5629320182384	39.0	35.0	39.0	27.0	39.0
13	34.57309082500088	39.0	35.0	39.0	27.0	39.0
14	35.536769538325935	40.0	36.0	41.0	25.0	41.0
15	35.63417001389161	40.0	36.0	41.0	26.0	41.0
16	35.54413460620045	40.0	36.0	41.0	25.0	41.0
17	35.38533923852518	40.0	36.0	41.0	25.0	41.0
18	35.22296321990472	40.0	35.0	41.0	24.0	41.0
19	35.553209900747866	40.0	36.0	41.0	26.0	41.0
20	35.59211077094257	40.0	36.0	41.0	26.0	41.0
21	35.59588915532632	40.0	36.0	41.0	26.0	41.0
22	35.52079100078255	40.0	36.0	41.0	25.0	41.0
23	35.27861814489169	40.0	35.0	41.0	24.0	41.0
24	35.42700402002135	40.0	35.0	41.0	25.0	41.0
25	35.34256614434876	40.0	35.0	41.0	25.0	41.0
26	35.39060531567808	40.0	35.0	41.0	25.0	41.0
27	35.36905739799552	40.0	35.0	41.0	25.0	41.0
28	35.26939237184611	40.0	35.0	41.0	25.0	41.0
29	35.23506551176933	40.0	35.0	41.0	25.0	41.0
30	35.018369436442946	39.0	35.0	41.0	24.0	41.0
31	34.929264716478734	39.0	35.0	41.0	24.0	41.0
32	34.94578953453884	39.0	35.0	41.0	24.0	41.0
33	34.936525220229804	39.0	35.0	41.0	24.0	41.0
34	34.929746984983986	39.0	35.0	41.0	24.0	41.0
35	34.8704416996362	39.0	35.0	41.0	24.0	41.0
36	34.748253159126826	39.0	35.0	41.0	23.0	41.0
37	34.659649475587464	39.0	34.0	41.0	22.0	41.0
38	34.567344155049824	39.0	34.0	41.0	22.0	41.0
39	34.50141379408576	39.0	34.0	41.0	21.0	41.0
40	34.472340610929294	39.0	34.0	41.0	21.0	41.0
41	34.3566025373557	39.0	34.0	41.0	19.0	41.0
42	34.18592238459824	39.0	34.0	41.0	18.0	41.0
43	34.09579782928901	39.0	33.0	41.0	16.0	41.0
44	34.03220072290004	39.0	33.0	41.0	15.0	41.0
45	33.95023619091666	39.0	33.0	41.0	15.0	41.0
46	33.80387691596161	39.0	33.0	41.0	9.0	41.0
47	33.624698582184216	38.0	33.0	41.0	7.0	41.0
48	33.457212997454604	38.0	33.0	41.0	2.0	41.0
49	33.336398201631134	38.0	33.0	40.0	2.0	41.0
50	33.15239885850832	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9100415528572583
1101	2	0.987540371947734
1101	3	1.0819405583681103
1101	4	1.0894633374371736
1101	5	0.9196389967747081
1101	6	0.9904311770920913
1101	7	1.041972496631253
1101	8	1.0576177636105228
1101	9	1.3081983254533611
1101	10	1.2910894717358943
1101	11	1.281950823741461
1101	12	1.2649234645353644
1101	13	1.266684969142979
1101	14	1.4929446193804523
1101	15	1.4025631618270893
1101	16	1.294046472741762
1101	17	1.534073534662241
1101	18	1.6606902833924124
1101	19	1.3520956519020046
1101	20	1.4269975431847968
1101	21	1.4198743793639963
1101	22	1.2716996756508507
1101	23	1.340030382141471
1101	24	1.284046747029727
1101	25	1.358548403430909
1101	26	1.3413990868543024
1101	27	1.3513826857579332
1101	28	1.3829803537809937
1101	29	1.257450010634713
1101	30	1.5434745409952768
1101	31	1.4602479023414077
1101	32	1.4583712119583012
1101	33	1.442764752581752
1101	34	1.413015480207335
1101	35	1.3832586428263127
1101	36	1.4357179444516177
1101	37	1.486476349670852
1101	38	1.3892107873833908
1101	39	1.4002209959471585
1101	40	1.4066502334699464
1101	41	1.3384135914153674
1101	42	1.3911710842320133
1101	43	1.344615007375424
1101	44	1.2375722548741166
1101	45	1.2765837042145733
1101	46	1.2469578258841736
1101	47	1.2268482717847107
1101	48	1.196323487684964
1101	49	1.3560388995676291
1101	50	1.3404107317267417
1102	1	0.6530135236024321
1102	2	0.6354187643317424
1102	3	0.5153515208657282
1102	4	0.5936290797146668
1102	5	0.5450795749440616
1102	6	0.6560881427403871
1102	7	0.7113639057816457
1102	8	0.6911342635711648
1102	9	0.8010149295881845
1102	10	0.9323077355429135
1102	11	0.684529222505212
1102	12	0.7539760695661215
1102	13	0.7521461691344982
1102	14	1.0740349665421505
1102	15	0.9499917552264634
1102	16	1.1221554052115223
1102	17	1.1664410938796834
1102	18	1.2668073557412285
1102	19	1.1190773688598767
1102	20	1.0665220313589856
1102	21	0.9307669233962557
1102	22	1.0000908295047424
1102	23	1.220825343694159
1102	24	1.1140185344769122
1102	25	1.1291941097261855
1102	26	0.9217877147959257
1102	27	0.9357827278067674
1102	28	1.0090257300972922
1102	29	0.8991925002899848
1102	30	1.1451098125122101
1102	31	1.1532226049827585
1102	32	1.0998153764203735
1102	33	0.9287397407674973
1102	34	0.8050645226963624
1102	35	0.8745355864852442
1102	36	1.0494362916001094
1102	37	1.04512233245746
1102	38	1.0695296215016938
1102	39	1.0101141026541711
1102	40	0.9764424059323673
1102	41	1.071527464197196
1102	42	1.166816426807003
1102	43	1.1446553308021095
1102	44	1.1535847664260928
1102	45	1.0110404131391846
1102	46	1.0135605920968018
1102	47	1.1943899369292197
1102	48	1.1684522355083615
1102	49	1.0957950078052505
1102	50	0.9941367132736119
1103	1	0.6766088277395745
1103	2	0.6375939734200671
1103	3	0.7650251158349306
1103	4	0.7010546460218521
1103	5	0.6997687649061675
1103	6	0.6891529800892258
1103	7	0.8070051400455966
1103	8	0.7658373970491539
1103	9	0.8756216023798302
1103	10	0.9499264963219574
1103	11	0.9854609028843768
1103	12	0.9633238417725636
1103	13	0.9231266002910985
1103	14	1.1409149425506442
1103	15	1.1060694225679768
1103	16	1.1698356854421874
1103	17	1.3622989009346114
1103	18	1.1094061364822707
1103	19	1.1239896499232387
1103	20	1.1514713817694329
1103	21	1.0682785880799486
1103	22	1.1296296989875643
1103	23	1.2819298906922043
1103	24	1.1242460466929458
1103	25	0.939394775778986
1103	26	1.0417768732743369
1103	27	1.1287319772220528
1103	28	1.1354636358492272
1103	29	1.1537659065115946
1103	30	1.2858384823080087
1103	31	1.2771033750399639
1103	32	1.1521274184961925
1103	33	1.134028641038384
1103	34	1.1401065980026814
1103	35	1.059187619502417
1103	36	1.1573487473438746
1103	37	1.0406511838384347
1103	38	1.0944082340754875
1103	39	1.1238213619906858
1103	40	1.1280575813831248
1103	41	1.1845695982456022
1103	42	1.1446480400449985
1103	43	1.1383280868931251
1103	44	1.0826037684599115
1103	45	0.9934163449612683
1103	46	0.8925067590984881
1103	47	0.8781256449542667
1103	48	0.9472319666035887
1103	49	0.7976954688275626
1103	50	0.8939804799397422
1104	1	0.771767672288874
1104	2	0.7679073111689902
1104	3	0.799051453164946
1104	4	0.6990053023250766
1104	5	0.7628134823778012
1104	6	0.8269431841766561
1104	7	0.8417435671290434
1104	8	0.9072083866312752
1104	9	1.0591556489017435
1104	10	1.061067472951457
1104	11	1.0352411546540807
1104	12	1.0566536994357563
1104	13	0.9654457580970046
1104	14	1.2435704830156666
1104	15	1.1687415874202287
1104	16	1.2703095159789228
1104	17	1.3769630507833241
1104	18	1.405267739219262
1104	19	1.2561904489892655
1104	20	1.1839758624424448
1104	21	1.0598139450782682
1104	22	1.1238396145876095
1104	23	1.3489932672381215
1104	24	1.2767243888388649
1104	25	1.2786471956466983
1104	26	1.1087808625186923
1104	27	1.1528192841626108
1104	28	1.330030382408971
1104	29	1.3244002805511812
1104	30	1.458348805352614
1104	31	1.4976385504784702
1104	32	1.3864200604551726
1104	33	1.4038645576263562
1104	34	1.2810102992870895
1104	35	1.3531155595288524
1104	36	1.2672769754316846
1104	37	1.241458414990447
1104	38	1.2818088452930425
1104	39	1.2169203648317435
1104	40	1.1940408291940088
1104	41	1.3347708354895076
1104	42	1.3543850724650852
1104	43	1.3109109324323782
1104	44	1.189562732899546
1104	45	1.2192941460792284
1104	46	1.2290218351558195
1104	47	1.2663111727100969
1104	48	1.234221126772816
1104	49	1.2500516891782851
1104	50	1.2672770381134342
1105	1	0.5812526753049632
1105	2	0.6363049331689474
1105	3	0.5877177069732227
1105	4	0.5492514505591828
1105	5	0.4696180227219884
1105	6	0.45125185896349507
1105	7	0.43403454149962073
1105	8	0.5329383294370587
1105	9	0.7125090720876841
1105	10	0.717458478637937
1105	11	0.6520951777434831
1105	12	0.5751528821890659
1105	13	0.6664739803760469
1105	14	0.9049623916206286
1105	15	0.8246505511409836
1105	16	0.8372972794749458
1105	17	0.723522449260841
1105	18	1.008085598163504
1105	19	0.802649070113219
1105	20	0.8235251785645517
1105	21	0.7990566103972156
1105	22	0.8618920646102168
1105	23	1.160108695975758
1105	24	0.8904812207379393
1105	25	0.821262939451664
1105	26	0.8288834637876832
1105	27	0.7892983761259131
1105	28	0.8757097718231535
1105	29	0.9149079658036428
1105	30	1.1555383686192826
1105	31	1.1540881604960234
1105	32	0.9528705924740279
1105	33	0.9193148086256429
1105	34	0.8694165647981578
1105	35	0.9167646337812911
1105	36	0.9726198725365407
1105	37	0.8996061947249387
1105	38	1.0403105409518645
1105	39	1.044678871197668
1105	40	1.0111328953426835
1105	41	1.0805652842381832
1105	42	1.1102104534535044
1105	43	1.0885171880605569
1105	44	1.0440235720492907
1105	45	0.9415183044818605
1105	46	0.8953690990115035
1105	47	1.0030085921710707
1105	48	1.1762438168431828
1105	49	1.0652211114370047
1105	50	1.0283950882273203
1106	1	0.45044586627356153
1106	2	0.5156998805911819
1106	3	0.5343323044839074
1106	4	0.617875826069195
1106	5	0.4787296345596843
1106	6	0.4522279226698842
1106	7	0.5241716534124023
1106	8	0.5339814880206077
1106	9	-0.47104975986897557
1106	10	-0.046136743461310914
1106	11	0.40338633723811057
1106	12	0.5874959853801087
1106	13	0.7095741992289462
1106	14	0.8928387691132755
1106	15	0.851865910688602
1106	16	0.8835671427220504
1106	17	0.9886782158055851
1106	18	-0.6048382190366937
1106	19	0.4206786701365246
1106	20	0.636612093209564
1106	21	0.7299858185334713
1106	22	0.9065861791633409
1106	23	-0.44224532386568427
1106	24	0.584586307991195
1106	25	0.7979347667345422
1106	26	0.9306833623410142
1106	27	0.8893470885762014
1106	28	1.0713578941131772
1106	29	1.0332461630251046
1106	30	0.253894357754227
1106	31	0.8746981274792631
1106	32	0.8733405528517579
1106	33	0.9931891620895001
1106	34	0.9947292450727261
1106	35	0.9029705548257283
1106	36	1.0269794259397003
1106	37	0.9290081213876888
1106	38	0.9570242181739133
1106	39	0.9803760826109809
1106	40	0.9824275684900883
1106	41	1.007325340303602
1106	42	1.1716022170080151
1106	43	1.0670423641870599
1106	44	1.0359628680572683
1106	45	1.0183096665853597
1106	46	0.9772529524821749
1106	47	1.054525490614246
1106	48	1.0471845154676132
1106	49	1.02296914251135
1106	50	1.0192184414429022
1107	1	0.41034773564889093
1107	2	0.3876646515124129
1107	3	0.44973596412424754
1107	4	0.4309021590809792
1107	5	0.47424152695888466
1107	6	0.43619414817542435
1107	7	0.5021248410912591
1107	8	0.324965525456939
1107	9	0.5880830903071086
1107	10	0.7101978962686317
1107	11	0.5424944164276155
1107	12	0.6546633573318061
1107	13	0.601658381374115
1107	14	0.8964545681899807
1107	15	0.768058847613112
1107	16	0.6595742280545238
1107	17	0.832984358137665
1107	18	0.864230241566041
1107	19	0.7341876222303867
1107	20	0.7661710993025608
1107	21	0.780151967504203
1107	22	0.8515565521946371
1107	23	0.9958937115611661
1107	24	0.8162784481425476
1107	25	0.781056552997903
1107	26	0.8110074763236881
1107	27	0.6999132367012422
1107	28	0.7661092049784912
1107	29	0.7207922074033846
1107	30	0.952019101044506
1107	31	1.0750256011519213
1107	32	0.9886341384386128
1107	33	0.8227884921504014
1107	34	0.6730692007240435
1107	35	0.5967400020587021
1107	36	0.7150648850745824
1107	37	0.5940732653536713
1107	38	0.6417590208142272
1107	39	0.6085140436289649
1107	40	0.7830570682979783
1107	41	0.695139986899008
1107	42	0.7817811166557291
1107	43	0.8150554285376188
1107	44	0.7990210201009234
1107	45	0.8199315050315548
1107	46	0.8872208528354619
1107	47	0.8678737618826844
1107	48	0.6617308198951193
1107	49	0.7976627197566373
1107	50	0.8679301406407092
1108	1	0.29322627519506383
1108	2	0.29371305469292963
1108	3	0.29097801726165073
1108	4	0.3070099304804117
1108	5	0.39631866708222674
1108	6	0.3541687786624834
1108	7	0.4154718372977584
1108	8	0.4193666403413232
1108	9	0.5168574290171932
1108	10	0.3728948701200352
1108	11	0.5133850306666474
1108	12	0.44493823841014546
1108	13	0.48811463752011264
1108	14	0.3447700337052808
1108	15	0.5086639484801552
1108	16	0.5165614460895895
1108	17	0.3625370087877968
1108	18	0.677464036342684
1108	19	0.5598547673913217
1108	20	0.5012830221474687
1108	21	0.5457877728852836
1108	22	0.5269222232262436
1108	23	0.3703005244150859
1108	24	0.4995248260141878
1108	25	0.5563630367737105
1108	26	0.4163949472523356
1108	27	0.3919567906387158
1108	28	0.389692943576037
1108	29	0.46724246429047156
1108	30	0.5448781448941418
1108	31	0.6282445323193286
1108	32	0.5984484788086277
1108	33	0.6788825008785082
1108	34	0.5885809326765497
1108	35	0.4404426324760209
1108	36	0.5535829222178847
1108	37	0.4389459975944874
1108	38	0.4157531430185273
1108	39	0.6426472402514491
1108	40	0.5290494695525183
1108	41	0.615594475291168
1108	42	0.657979369613173
1108	43	0.6678711625131228
1108	44	0.6357925263118887
1108	45	0.5805900667613457
1108	46	0.6272461137705392
1108	47	0.8449504723370609
1108	48	0.7795500779141307
1108	49	0.6703372134412646
1108	50	0.7166452125091212
1109	1	-0.1616005463162864
1109	2	-0.16440892492780534
1109	3	-0.16767859210445835
1109	4	-0.21461196545444494
1109	5	-0.2608003900496101
1109	6	-0.21324541131063768
1109	7	-0.23725969862498886
1109	8	-0.21203786474949027
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2211	8	-0.08815872577591932
2211	9	-0.03929666581537106
2211	10	-0.17630163473612726
2211	11	-0.12369808522083048
2211	12	-0.013268791595073992
2211	13	-0.08984231050994396
2211	14	-9.9106225171397E-5
2211	15	-0.15110550503415965
2211	16	-0.06758718988988477
2211	17	0.026819160370266104
2211	18	0.2282305387511343
2211	19	0.03195891972195852
2211	20	0.052383475204564434
2211	21	-3.9671048645573137E-4
2211	22	0.03432538862210066
2211	23	0.3175386310890147
2211	24	0.10385713254125761
2211	25	0.07517368107539824
2211	26	0.09564040629015835
2211	27	0.018848292469883177
2211	28	-0.08179433988253493
2211	29	-0.0012267938689376479
2211	30	0.12536029740027033
2211	31	0.1107208749801245
2211	32	0.03713567544279783
2211	33	0.020175399289342977
2211	34	-0.00579053719584266
2211	35	0.04234584174591305
2211	36	0.11865813154319227
2211	37	0.0629650435421425
2211	38	0.028927775515789733
2211	39	-0.08227154660850289
2211	40	-0.16759088809010336
2211	41	0.08032289504095047
2211	42	0.18142495888758958
2211	43	0.05640697064967526
2211	44	0.1301252484876727
2211	45	0.03600615421382969
2211	46	-0.134541083325459
2211	47	-0.01451514196864423
2211	48	0.053676453595905116
2211	49	0.01305458617193267
2211	50	0.034249967530392667
2212	1	-0.27416544646116137
2212	2	-0.29747008575668943
2212	3	-0.4220243081262609
2212	4	-0.34642453685330565
2212	5	-0.19694626027671092
2212	6	-0.26736385787562256
2212	7	-0.21622645814595387
2212	8	-0.2039790364962215
2212	9	-0.3571248128835762
2212	10	-0.10029498295244821
2212	11	-0.251290686250492
2212	12	-0.20911324920134433
2212	13	-0.3385853056350072
2212	14	-0.38488793487339734
2212	15	-0.35331881771355
2212	16	-0.35233729240025013
2212	17	-0.1122737368004465
2212	18	0.033163793113523354
2212	19	-0.22805930434292065
2212	20	-0.2985814890307097
2212	21	-0.23028053207286092
2212	22	-0.14058007404134543
2212	23	0.1281122335310414
2212	24	-0.04767004409549003
2212	25	-0.22101065534084086
2212	26	-0.20934569786037116
2212	27	-0.1447760184326654
2212	28	-0.2783376903768584
2212	29	-0.21526023502799063
2212	30	-0.04765077371914117
2212	31	-0.1683679217759888
2212	32	-0.2727235436560207
2212	33	-0.22502984924133784
2212	34	-0.24388065519666213
2212	35	-0.12552513187475256
2212	36	-0.13709247212895548
2212	37	-1.4712378814202722
2212	38	-0.7933279081144491
2212	39	-0.3902427525859977
2212	40	-0.3508593741259176
2212	41	-1.20650475086547
2212	42	-0.6651671409802873
2212	43	-0.40694762409268037
2212	44	-0.30706414510656543
2212	45	-0.2564440713089695
2212	46	-0.2352281305713504
2212	47	-0.05373196278053172
2212	48	-0.13844209394612506
2212	49	-0.03234839346266938
2212	50	0.025332021382503456
2213	1	-0.9238708647635008
2213	2	-0.927878773690999
2213	3	-0.9247382779714464
2213	4	-0.9535368101575656
2213	5	-1.0301752042708472
2213	6	-0.9473535046070438
2213	7	-0.9046447485533022
2213	8	-0.8877297283203518
2213	9	-1.0098549826569823
2213	10	-1.1862292231758147
2213	11	-1.0220489438541307
2213	12	-1.0371163625246638
2213	13	-1.0102410300838685
2213	14	-2.795789706402097
2213	15	-1.6878066751101244
2213	16	-1.2672772975952427
2213	17	-0.9313403639386806
2213	18	-1.0233675314899031
2213	19	-1.0565086729128552
2213	20	-1.213198872425643
2213	21	-1.2232782163550766
2213	22	-1.2045050826002281
2213	23	-1.745907028321298
2213	24	-1.3344875245982095
2213	25	-1.3706767899645413
2213	26	-1.4112583908024376
2213	27	-1.4225166745618836
2213	28	-1.2770161800846296
2213	29	-1.5945417659166168
2213	30	-1.2775879657254663
2213	31	-1.1355265828633918
2213	32	-1.2433861620233415
2213	33	-1.2456032073809098
2213	34	-1.1969603395688253
2213	35	-1.4186668257172173
2213	36	-1.3214767509476175
2213	37	-1.4015923248357112
2213	38	-1.2806290000722171
2213	39	-1.2923849062677917
2213	40	-1.5003196365739342
2213	41	-1.6396834888497551
2213	42	-1.3267040139443935
2213	43	-1.3336590570199292
2213	44	-1.288612656319394
2213	45	-1.3880347574057623
2213	46	-1.2781979583197653
2213	47	-1.3056627831210292
2213	48	-1.2727171953448249
2213	49	-1.5416895566083397
2213	50	-1.4097419705276302
2214	1	-0.8565508255233532
2214	2	-0.8623612992331644
2214	3	-1.0214391874031286
2214	4	-1.1725308516366368
2214	5	-0.9794483730109391
2214	6	-0.9473171644068046
2214	7	-1.0398981587631226
2214	8	-0.9857576001637298
2214	9	-1.1836047480355703
2214	10	-1.3833322914334332
2214	11	-1.2107446159361572
2214	12	-1.1623666783664248
2214	13	-1.2458876957705627
2214	14	-1.7220621630559592
2214	15	-1.4532246864399099
2214	16	-1.1716843100183212
2214	17	-1.8180434301612465
2214	18	-1.788518714899432
2214	19	-1.5083609056638423
2214	20	-1.4170095239927178
2214	21	-1.518341927085629
2214	22	-1.4597218546201631
2214	23	-1.242153449038696
2214	24	-1.2415054895579019
2214	25	-1.2432433365934656
2214	26	-1.4250100676755508
2214	27	-1.3465024241157622
2214	28	-1.2577768604240518
2214	29	-1.274452376292075
2214	30	-1.1696000626513197
2214	31	-1.1187393182138905
2214	32	-1.1283103754442507
2214	33	-1.2691283085565672
2214	34	-1.3329358295573712
2214	35	-1.266137738566698
2214	36	-1.3784988859452838
2214	37	-1.474543096401753
2214	38	-1.3287321597254333
2214	39	-1.271178854970323
2214	40	-1.275916810541922
2214	41	-1.270399643358843
2214	42	-1.119221275993759
2214	43	-1.2603968499378482
2214	44	-1.1973006351810298
2214	45	-1.1839502824298975
2214	46	-1.243501425338394
2214	47	-1.1622291029871121
2214	48	-1.2682997714543731
2214	49	-1.3186920969700395
2214	50	-1.1911446731846596
2215	1	-0.7105270371544172
2215	2	-0.9330579931248799
2215	3	-0.7973916244236143
2215	4	-0.7274859285001369
2215	5	-0.8123175854716109
2215	6	-0.8188907774531557
2215	7	-0.8852259839433785
2215	8	-0.9742729785265496
2215	9	-0.9809756768094715
2215	10	-1.3907071069434949
2215	11	-1.0629310245743255
2215	12	-1.0739881872774362
2215	13	-1.1006984692696449
2215	14	-1.2145180526273478
2215	15	-1.2469517476286143
2215	16	-1.1236041336898595
2215	17	-1.0626522301245345
2215	18	-1.1234319663543175
2215	19	-1.3229217283868167
2215	20	-1.2814825239280339
2215	21	-1.24693817942633
2215	22	-1.5033855141068457
2215	23	-1.4578468782566034
2215	24	-1.2615425247089078
2215	25	-1.1964679811349654
2215	26	-1.3136039861289248
2215	27	-1.3297544335281586
2215	28	-1.3305661670254523
2215	29	-1.3506412137838026
2215	30	-1.1833772126527435
2215	31	-1.300766212951622
2215	32	-1.4034473361239534
2215	33	-1.4036764098318457
2215	34	-1.3389857319611167
2215	35	-1.3090758963410067
2215	36	-1.3419169218272557
2215	37	-1.3601178522307507
2215	38	-1.3156878520237854
2215	39	-1.4674050464042878
2215	40	-1.412844981929453
2215	41	-1.5188838959532376
2215	42	-1.4299112601691348
2215	43	-1.6552845460814893
2215	44	-1.5746897971351075
2215	45	-1.5412342885818973
2215	46	-1.415644030384506
2215	47	-1.435978818486916
2215	48	-1.5427524216035735
2215	49	-1.5558796050184682
2215	50	-1.5862769577004592
2216	1	-1.4517554312276673
2216	2	-1.7323686592570517
2216	3	-1.4968689414342862
2216	4	-2.67381342952611
2216	5	-1.7823187001110519
2216	6	-1.7000538238702099
2216	7	-1.7901386421173981
2216	8	-1.6475867831517483
2216	9	-1.7243406550532754
2216	10	-2.122937819804548
2216	11	-1.880363486405983
2216	12	-1.9423954442864257
2216	13	-2.0814302147074955
2216	14	-2.059825597154969
2216	15	-2.1745908061912473
2216	16	-2.2323076560272526
2216	17	-2.1018134593415354
2216	18	-1.8663354285994416
2216	19	-2.202615630894556
2216	20	-2.373742005540862
2216	21	-2.3928573017414294
2216	22	-2.6496371091082835
2216	23	-4.299714393686912
2216	24	-3.034233420914333
2216	25	-2.684339713771344
2216	26	-2.6937052847452776
2216	27	-2.7324495963768243
2216	28	-3.111966335326798
2216	29	-2.7486376186877806
2216	30	-2.479523745978234
2216	31	-2.379263774178874
2216	32	-2.5135922515334173
2216	33	-2.710691675632667
2216	34	-2.862192993330403
2216	35	-2.9569650210373766
2216	36	-2.829596164216344
2216	37	-2.714137278891542
2216	38	-2.8951999500838035
2216	39	-2.8075182329726687
2216	40	-2.9918868126894154
2216	41	-2.936478187771062
2216	42	-3.196330352008207
2216	43	-3.136618135403179
2216	44	-3.070058631478652
2216	45	-3.1449700272232057
2216	46	-3.120039655532185
2216	47	-3.1997038912877613
2216	48	-3.264682161857273
2216	49	-3.5674176585171686
2216	50	-3.506434382864935
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106922.0
3	5339.0
4	11021.0
5	8926.0
6	7145.0
7	6161.0
8	5636.0
9	5125.0
10	5028.0
11	4917.0
12	5069.0
13	5326.0
14	5607.0
15	6023.0
16	6510.0
17	7193.0
18	7719.0
19	8945.0
20	11627.0
21	15968.0
22	20439.0
23	24019.0
24	25019.0
25	26057.0
26	28364.0
27	32055.0
28	37382.0
29	42561.0
30	49123.0
31	59055.0
32	72288.0
33	90426.0
34	114571.0
35	149305.0
36	198579.0
37	276818.0
38	452810.0
39	1038737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.425128413043815	18.157740212120878	13.076282562687396	24.340848812147904
2	15.470218584182978	20.482751173274362	39.4955641274843	24.551466115058357
3	18.285043004042016	25.619523469539345	29.684241308226987	26.411192218191648
4	12.35442126036542	16.85057560703366	38.493527523286566	32.30147560931436
5	13.49986868712956	38.04732562807969	34.81793462128395	13.634871063506795
6	31.324717378043566	37.656435121950565	17.8090648953198	13.20978260468607
7	27.58790534330771	32.62374678996164	22.222032221257688	17.56631564547295
8	25.962781448736887	34.91843875906865	20.434490286256565	18.6842895059379
9	26.647691682438168	15.997937074052066	19.436839994473825	37.91753124903593
10	14.325741618322066	29.001563102071803	34.259535308299334	22.413159971306797
11	34.09533677366569	23.632333759183442	24.199217374684384	18.073112092466477
12	23.252808887022745	25.256909784940973	30.557055402366235	20.93322592567005
13	27.51553971913645	22.57525885558583	26.263307482708026	23.645893942569693
14	21.933357904052794	21.660609503636813	27.6666748270967	28.739357765213697
15	23.400197685131925	29.401923656779168	24.038364465778905	23.159514192310002
16	23.148504650454583	28.662710907137136	25.49422399186489	22.694560450543392
17	22.191624322490384	28.14985159273249	26.912917520174766	22.745606564602358
18	22.216953179261896	27.887061976801082	27.760560229688537	22.135424614248482
19	23.00736326843802	27.457519347192395	27.890039264504864	21.645078119864728
20	23.369648158415416	27.005256207849467	27.535539189790658	22.08955644394446
21	23.48673843877223	26.83471329952902	27.5735089422212	22.105039319477545
22	23.148349689484114	26.584935661484195	27.460390233464615	22.80632441556708
23	23.010849045371877	26.806623119342852	27.36321144353107	22.819316391754203
24	22.824326417937776	26.80810665979889	27.5493101706817	22.818256751581632
25	22.967057292540098	26.74728496012128	27.486567344392494	22.799090402946128
26	22.886177580068548	26.97381440989005	27.548236348548162	22.591771661493233
27	22.813319528280402	27.073332132602296	27.370563374282288	22.742784964835014
28	22.740804205938552	26.96992346007686	27.470170363054724	22.819101970929857
29	22.948609059328824	27.16000681616612	27.320213555314933	22.571170569190123
30	23.130095657239796	27.04992616338368	27.277858775019848	22.54211940435668
31	23.347308877640035	26.709723551190166	27.169672230388876	22.77329534078092
32	22.831906670383002	27.047698204185703	27.36643754865529	22.753957576776
33	22.972105922833553	26.89512662798182	27.21147438109775	22.92129306808688
34	23.09137656569681	26.818364582715038	27.38916001755881	22.70109883402935
35	23.24735376530402	26.910491224610134	27.23117884649055	22.610976163595296
36	23.279826245922397	26.85869063891521	27.123179230014504	22.738303885147886
37	23.26923851056692	26.680442804428044	27.386279771888628	22.664038913116404
38	23.086948851367715	26.74448088340747	27.329915419718493	22.838654845506323
39	23.03976387735277	27.12546765301479	27.14619075514917	22.68857771448327
40	23.257097633116253	26.660037961354536	27.390726966646323	22.692137438882888
41	23.02213637941181	26.900789519129365	27.26806932477588	22.809004776682947
42	23.08476240490462	27.00594834483901	27.142500719314448	22.766788530941923
43	23.050307612358136	26.748998373929222	27.18341072536167	23.017283288350963
44	23.057387068849327	26.991495505015994	27.171938586321325	22.779178839813348
45	23.23925174171255	26.81632145346621	27.021715959448162	22.922710845373075
46	23.27663116928139	26.700151307842223	26.888305442690267	23.134912080186115
47	23.031123066793434	27.0376697862594	26.89555823455815	23.035648912389018
48	22.98070420320386	27.09693297117181	26.89538814060728	23.02697468501705
49	23.230600956573287	26.860435796120598	26.860502858185193	23.048460389120923
50	23.289968134927662	26.98375498227036	26.780641968553358	22.945634914248622
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2168.0
1	1729.5
2	1291.0
3	1316.0
4	1341.0
5	1310.0
6	1279.0
7	1257.0
8	1235.0
9	1408.0
10	1581.0
11	1916.5
12	2252.0
13	3830.5
14	5409.0
15	7873.0
16	10337.0
17	11543.0
18	12749.0
19	13907.5
20	15066.0
21	17852.5
22	20639.0
23	25581.0
24	30523.0
25	37467.0
26	44411.0
27	52602.0
28	60793.0
29	68289.0
30	75785.0
31	83839.0
32	91893.0
33	102429.0
34	112965.0
35	123228.5
36	133492.0
37	141671.5
38	149851.0
39	158175.0
40	166499.0
41	174572.0
42	182645.0
43	188650.0
44	194655.0
45	199287.5
46	203920.0
47	206684.0
48	209448.0
49	208317.5
50	207187.0
51	202697.0
52	198207.0
53	190409.5
54	182612.0
55	171467.0
56	160322.0
57	149417.5
58	138513.0
59	125017.0
60	111521.0
61	98081.5
62	84642.0
63	72688.5
64	60735.0
65	51019.5
66	41304.0
67	33591.5
68	25879.0
69	21155.5
70	16432.0
71	12946.0
72	9460.0
73	7899.0
74	6338.0
75	4891.5
76	3445.0
77	2770.0
78	2095.0
79	1640.5
80	1186.0
81	948.5
82	711.0
83	585.0
84	459.0
85	340.5
86	222.0
87	171.5
88	121.0
89	94.5
90	68.0
91	54.0
92	40.0
93	34.5
94	29.0
95	20.0
96	11.0
97	15.5
98	20.0
99	24.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06153196495091016
2	0.06665962869681935
3	0.06320767205741644
4	0.0759765602089942
5	0.07996474312247911
6	0.061263851813869154
7	0.046048431286792244
8	0.056974041621213105
9	0.055063735519795966
10	0.0644812094583612
11	0.05177934959104369
12	0.06800019438202436
13	0.0650174357324432
14	0.06521852058522394
15	0.07728361175206908
16	0.07275920256450216
17	0.0835172421882724
18	0.06830182166119549
19	0.06659260041255909
20	0.08003177140673935
21	0.07845660672662347
22	0.07252460356959128
23	0.06964238734640049
24	0.05942057399671226
25	0.0762446733460352
26	0.08569566142673055
27	0.07701549861502809
28	0.09675532832967192
29	0.08938221706104434
30	0.0748035652344398
31	0.07299380155941303
32	0.07983068655395861
33	0.07661332890946658
34	0.06153196495091016
35	0.08073556839147199
36	0.08680162811702467
37	0.09434231009630288
38	0.07604358849325445
39	0.054829136524885094
40	0.06149845080878003
41	0.054292910250803086
42	0.060627083113396776
43	0.039043975581596044
44	0.03877586244455504
45	0.0422948473682182
46	0.03867532001816466
47	0.031737892597228715
48	0.04531112015992948
49	0.05037175562157841
50	0.041524022099225316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2983815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.79957485805627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.35986137726341	33.35932172566115
2	13.899428635902748	13.565724164038942
3	6.338163250043253	9.278990159492007
4	3.5473301428418003	6.924328114073913
5	2.1752504113518976	5.30756476418923
6	1.409841581511876	4.127980187699354
7	0.968887919650231	3.30969229948265
8	0.6789055942386872	2.6504243494083193
9	0.4894108925197916	2.149473913728093
>10	2.0678072880234977	16.122824715425686
>50	0.047914270202062986	1.5793211346449498
>100	0.016784235978100748	1.307399440503215
>500	1.3813349088417134E-4	0.04823082202374022
>1k	2.762669817542799E-4	0.26872420962870236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3493	0.11706489846052788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.702828426025072E-5	0.0	0.0	0.0	3.351414213012536E-5
3	6.702828426025072E-5	0.0	0.0	0.0	3.351414213012536E-5
4	6.702828426025072E-5	0.0	0.0	0.0	3.351414213012536E-5
5	6.702828426025072E-5	0.0	0.0	0.0	6.702828426025072E-5
6	1.0054242639037608E-4	0.0	0.0	0.0	6.702828426025072E-5
7	1.3405656852050145E-4	0.0	0.0	0.0	6.702828426025072E-5
8	1.3405656852050145E-4	0.0	0.0	0.0	6.702828426025072E-5
9	1.3405656852050145E-4	0.0	0.0	0.0	6.702828426025072E-5
10	1.3405656852050145E-4	0.0	0.0	0.0	6.702828426025072E-5
11	1.3405656852050145E-4	0.0	0.0	0.0	6.702828426025072E-5
12	1.675707106506268E-4	0.0	0.0	0.0	3.351414213012536E-4
13	2.681131370410029E-4	0.0	0.0	0.0	3.6865556343137893E-4
14	2.681131370410029E-4	0.0	0.0	0.0	4.69197989821755E-4
15	2.681131370410029E-4	0.0	0.0	3.351414213012536E-5	6.367687004723818E-4
16	3.351414213012536E-4	0.0	0.0	3.351414213012536E-5	6.702828426025072E-4
17	3.6865556343137893E-4	0.0	0.0	6.702828426025072E-5	7.373111268627579E-4
18	4.021697055615043E-4	0.0	0.0	6.702828426025072E-5	8.043394111230086E-4
19	6.702828426025072E-4	0.0	0.0	1.0054242639037608E-4	8.043394111230086E-4
20	6.702828426025072E-4	0.0	0.0	1.0054242639037608E-4	9.048818375133847E-4
21	6.702828426025072E-4	0.0	0.0	1.3405656852050145E-4	9.719101217736354E-4
22	6.702828426025072E-4	0.0	0.0	2.0108485278075216E-4	0.001038938406033886
23	6.702828426025072E-4	0.0	0.0	3.351414213012536E-4	0.0010724525481640116
24	8.713676953832594E-4	0.0	0.0	4.021697055615043E-4	0.0011059666902941368
25	8.713676953832594E-4	0.0	0.0	4.69197989821755E-4	0.0011059666902941368
26	8.713676953832594E-4	0.0	0.0	6.032545583422564E-4	0.0012065091166845129
27	8.713676953832594E-4	0.0	0.0	6.702828426025072E-4	0.0012735374009447637
28	8.713676953832594E-4	0.0	0.0	0.0013405656852050144	0.001307051543074889
29	9.719101217736354E-4	0.0	0.0	0.0025470748018895273	0.0013405656852050144
30	0.001038938406033886	0.0	0.0	0.003921154629224667	0.0013405656852050144
31	0.001038938406033886	0.0	0.0	0.00750716783714808	0.0013405656852050144
32	0.001038938406033886	0.0	0.0	0.010892096192290741	0.0013405656852050144
33	0.0011059666902941368	0.0	0.0	0.01481325082151541	0.0013405656852050144
34	0.0011059666902941368	0.0	0.0	0.019773343856773963	0.0015416505379857665
35	0.001307051543074889	0.0	0.0	0.024934521744813268	0.0015416505379857665
36	0.0015081363958556412	0.0	0.0	0.031235180465276836	0.0015416505379857665
37	0.0015081363958556412	0.0	0.0	0.040552111977451684	0.0015416505379857665
38	0.0016086788222460173	0.0	0.0	0.05164529302252318	0.0016086788222460173
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5345	0.0	23.298706	1
GGTATCA	2600	0.0	21.748177	1
TATCAAC	6725	0.0	18.123281	2
TCAACGC	6810	0.0	17.734953	4
CAACGCA	6890	0.0	17.560667	5
ATCAACG	6895	0.0	17.515734	3
AACGCAG	7110	0.0	17.047094	6
GTATAGA	650	0.0	15.57068	1
TTATGCG	100	4.947634E-4	15.399625	18
ACGCAGA	8200	0.0	14.78207	7
GTACATG	7810	0.0	14.705594	1
GTATTAC	365	0.0	14.4671	1
CGCAGAG	8355	0.0	14.323526	8
AGAGTAC	7715	0.0	14.230509	11
TTAGGCG	140	2.7564574E-5	14.14441	30
GAGTACT	4100	0.0	14.006181	12
TACATGG	7930	0.0	13.98224	2
TAGCCCG	95	0.0062450655	13.893931	5
AGTACTT	4375	0.0	13.427535	13
CAGAGTA	8495	0.0	13.260576	10
>>END_MODULE