FastQCFastQC Report
Mon 6 Feb 2017
SRR2077434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983815
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42660.1429713303271148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64600.023.3974881
CGCGATA500.00257929122.00297712
GGTATCA29900.021.7068021
TATCAAC88150.017.0712072
ATCAACG89850.016.700083
TCAACGC90300.016.51944
CAACGCA91150.016.2943045
AACGCAG93250.016.0203956
GTACATG77050.014.7340471
GCGATAC1201.1805797E-414.66865114
TACATGG77050.014.3052542
GAGTACT70050.013.97762312
CGTATAC950.006239847713.8956853
ACGCAGA107950.013.7371567
ACATGGG76950.013.7241333
TCGTTAC2256.721166E-913.69028223
TGCGATA1453.8346192E-513.6570213
TAGCCCT10200.013.5891624
CGCAGAG109500.013.5427048
AGTACTT73100.013.42452413
Produced by FastQC (version 0.11.5)