FastQCFastQC Report
Mon 6 Feb 2017
SRR2077433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94604
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20212.13627330768255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.6934167688469832No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA6540.6913026933322058No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT4930.5211196143926261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.4809521796118557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.3467083844234916No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2400.25368906177328654No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC2390.25263202401589785No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.21774977802207096No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT1910.20189421166124052No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.19660902287429705No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1600.16912604118219104No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.13107268191619806No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA1170.12367341761447719No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1150.12155934209969979No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1030.10887488901103548No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG1010.10676081349625809No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG980.10358970022409199No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC980.10358970022409199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT250.002347126435.18391442
AGTTTTA353.194273E-431.43084116
TAAGTCC300.00571656729.33545121
ATATAGT300.00571656729.3354514
AGGGGTC300.00571656729.33545113
CCGCGAT300.00571656729.33545111
ACCCTGG300.005731386629.3199276
ACCTGAT406.959376E-427.51655235
TGAACTT450.00138812124.44620710
ATAAGGC450.00138812124.4462073
TAAGGCT551.5777329E-424.0017344
CATGGGG952.6175258E-823.1595674
CCTGATG500.002554079822.0132436
TTTAAGT703.1733136E-522.00158919
TATAAGG500.00256195522.0015892
CTATGAT500.002569850321.9899469
CTTTCCA500.002569850321.9899469
GGGATGG500.002569850321.9899465
AAGGCTA602.854547E-421.9899445
GTACATG2450.020.6545541
Produced by FastQC (version 0.11.5)