##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077433_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94604
Sequences flagged as poor quality	0
Sequence length	50
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.74113145321551	31.0	28.0	34.0	10.0	34.0
2	27.804955393006637	31.0	28.0	34.0	10.0	34.0
3	27.927233520781364	31.0	28.0	34.0	10.0	34.0
4	31.0	35.0	32.0	37.0	10.0	37.0
5	31.309743774047607	35.0	32.0	37.0	10.0	37.0
6	31.24439769988584	37.0	32.0	37.0	8.0	37.0
7	31.136125322396516	37.0	32.0	37.0	8.0	37.0
8	30.912836666525727	36.0	32.0	37.0	8.0	37.0
9	31.940710752188068	38.0	32.0	39.0	7.0	39.0
10	31.999059236395922	38.0	32.0	39.0	7.0	39.0
11	32.098843600693414	38.0	32.0	39.0	7.0	39.0
12	32.125322396516005	38.0	32.0	39.0	7.0	39.0
13	32.06285146505433	38.0	32.0	39.0	7.0	39.0
14	32.97898608938311	39.0	32.0	41.0	7.0	41.0
15	33.06510295547757	39.0	32.0	41.0	7.0	41.0
16	33.05149887953998	39.0	32.0	41.0	7.0	41.0
17	32.93079573802376	39.0	32.0	41.0	2.0	41.0
18	32.78959663439178	38.0	31.0	41.0	2.0	41.0
19	33.08133905543106	39.0	32.0	41.0	2.0	41.0
20	33.108864318633465	39.0	32.0	41.0	2.0	41.0
21	33.123092046847916	39.0	32.0	41.0	2.0	41.0
22	33.04664707623356	39.0	32.0	41.0	2.0	41.0
23	32.81874973574056	39.0	31.0	41.0	2.0	41.0
24	32.94572111115809	39.0	32.0	41.0	2.0	41.0
25	32.81930996575198	39.0	31.0	41.0	2.0	41.0
26	32.794670415627245	39.0	31.0	41.0	2.0	41.0
27	32.74820303581244	39.0	31.0	41.0	2.0	41.0
28	32.62291235042916	39.0	31.0	41.0	2.0	41.0
29	32.55304215466577	39.0	31.0	41.0	2.0	41.0
30	32.291731850661705	38.0	31.0	41.0	2.0	41.0
31	32.11747917635618	38.0	30.0	41.0	2.0	41.0
32	32.04559003847618	38.0	30.0	41.0	2.0	41.0
33	31.99085662339859	38.0	30.0	41.0	2.0	41.0
34	31.956862289120966	38.0	30.0	41.0	2.0	41.0
35	31.87140078643609	38.0	30.0	41.0	2.0	41.0
36	31.737685510126422	38.0	30.0	41.0	2.0	41.0
37	31.64583949938692	38.0	30.0	41.0	2.0	41.0
38	31.554289459219483	38.0	30.0	40.0	2.0	41.0
39	31.50869942074331	38.0	30.0	40.0	2.0	41.0
40	31.499196651304384	38.0	30.0	40.0	2.0	41.0
41	31.395797217876623	38.0	30.0	40.0	2.0	41.0
42	31.225814976110946	38.0	30.0	40.0	2.0	41.0
43	31.156494439981397	38.0	30.0	40.0	2.0	41.0
44	31.07016616633546	38.0	30.0	40.0	2.0	41.0
45	30.991913661155976	38.0	30.0	40.0	2.0	41.0
46	30.80749228362437	38.0	29.0	40.0	2.0	41.0
47	30.599763223542347	38.0	28.0	40.0	2.0	41.0
48	30.410627457612787	38.0	28.0	40.0	2.0	41.0
49	30.325451355122404	38.0	28.0	40.0	2.0	41.0
50	30.13488858822037	38.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.5425141770234347
1101	2	-0.6082033024237887
1101	3	-0.2827493179137477
1101	4	-0.8634590473618324
1101	5	-0.7623906060420396
1101	6	-1.0252707959458611
1101	7	-1.572329603397101
1101	8	-0.937322600004844
1101	9	-0.6929944139246516
1101	10	-0.3149559648626763
1101	11	-0.6556742667661588
1101	12	-1.2692743270160136
1101	13	-0.7535270864238832
1101	14	-0.5150125323335288
1101	15	-0.4641001051828155
1101	16	-0.5980094644557852
1101	17	-0.3638233406741236
1101	18	-0.7394918882660448
1101	19	-0.7792456054417656
1101	20	-0.7509759170648636
1101	21	-1.0148486864189508
1101	22	-1.1570726859481262
1101	23	-0.9470712721047754
1101	24	-0.6388258078967155
1101	25	-0.24138006722544247
1101	26	-0.39823413170849165
1101	27	-0.040653923619522914
1101	28	-0.3408664119191087
1101	29	-0.44019751315707367
1101	30	-0.27878763848423915
1101	31	-0.13392735962013091
1101	32	-0.3319210711914984
1101	33	0.1729980116081009
1101	34	-0.5578782159224289
1101	35	-0.8801965236333196
1101	36	-0.6071179780328357
1101	37	-0.6886022787363579
1101	38	-0.5416480701401234
1101	39	-0.7601318987726913
1101	40	-1.2511318994328562
1101	41	-1.0626698900136375
1101	42	-1.0672468655130878
1101	43	-1.1506453171193378
1101	44	-1.7031502734703352
1101	45	-1.6908010872234698
1101	46	-1.1472187437600248
1101	47	-1.5280322452533177
1101	48	-1.0997604814899091
1101	49	-1.1568989669987708
1101	50	-1.5807820263202146
1102	1	1.373518710951899
1102	2	0.7675932032288344
1102	3	1.2118549986330152
1102	4	1.3035502023812278
1102	5	0.8602198770000982
1102	6	0.933208135194942
1102	7	0.9356354531291053
1102	8	0.7678675336025549
1102	9	1.1796160691174862
1102	10	1.7976853506563373
1102	11	0.6494644793797804
1102	12	0.2693176565399966
1102	13	1.48604125889986
1102	14	1.488379040122794
1102	15	1.3674004086918004
1102	16	1.6213636085144145
1102	17	1.2802157137965864
1102	18	1.4983806708295333
1102	19	1.309038053345489
1102	20	1.3882841034901219
1102	21	1.4464565551021167
1102	22	1.4608615381012058
1102	23	1.087974976610539
1102	24	0.989026196214283
1102	25	1.2012201383244019
1102	26	1.2377884787745472
1102	27	1.2595516262263189
1102	28	0.9133473599205573
1102	29	1.215146782834708
1102	30	1.56314453006663
1102	31	1.707902034007823
1102	32	1.9467531323028524
1102	33	2.0299867063665786
1102	34	1.1574352475924776
1102	35	1.472372849439651
1102	36	1.638822412511871
1102	37	1.2754264982420587
1102	38	1.3432440161908126
1102	39	1.7703408658727362
1102	40	1.8732771447603582
1102	41	1.6379981469853355
1102	42	1.279258787107672
1102	43	0.842211825737806
1102	44	0.7093368796642991
1102	45	0.9730221399091086
1102	46	1.2892663538761546
1102	47	1.2356368194948857
1102	48	1.6005992307403076
1102	49	1.5858554009354506
1102	50	2.128776041511234
1103	1	1.6218721408840082
1103	2	1.6772091724253073
1103	3	1.8545746257482243
1103	4	1.8307059425778043
1103	5	1.8426395750444797
1103	6	1.8658760853418634
1103	7	1.7641895112910255
1103	8	1.9989953074398237
1103	9	1.7535850629365157
1103	10	2.049027938557849
1103	11	2.6483498781030548
1103	12	2.2748906629236245
1103	13	2.6506982656887956
1103	14	2.2741222765195914
1103	15	3.4009904380767395
1103	16	2.8668798715603145
1103	17	2.523198792122656
1103	18	1.9919970453355589
1103	19	1.5408751189043102
1103	20	1.6101910849472105
1103	21	1.9693565449693793
1103	22	1.8303518613355791
1103	23	2.5829086071708787
1103	24	2.455942803773304
1103	25	2.2724026289516175
1103	26	2.1242004759373856
1103	27	2.660352112597785
1103	28	2.7600390206764587
1103	29	2.593705907366065
1103	30	2.403908538578129
1103	31	2.5419277711645805
1103	32	2.6225054881646415
1103	33	2.516358172573895
1103	34	2.3093250033934645
1103	35	1.7675901966886158
1103	36	2.1156184404782294
1103	37	1.8888625099960343
1103	38	1.7048308030993162
1103	39	1.9126045197383768
1103	40	2.2729123661607034
1103	41	2.269825079805276
1103	42	2.078326575130774
1103	43	2.2717892905265415
1103	44	2.158318539406931
1103	45	1.5941083695169738
1103	46	1.9560005721353484
1103	47	1.8149255012255558
1103	48	1.6699578283692418
1103	49	1.8537650169046458
1103	50	2.0230409113055394
1104	1	1.099928887158761
1104	2	0.6923142959198998
1104	3	0.8223231458543694
1104	4	1.1976175571930305
1104	5	1.2527232655935663
1104	6	1.4852882102653204
1104	7	1.4292231916339553
1104	8	1.6789299879868729
1104	9	1.6011049649573366
1104	10	1.969930163501715
1104	11	1.415547265324939
1104	12	1.917164596379429
1104	13	0.9682120440108974
1104	14	1.412109620875583
1104	15	1.3413864372602475
1104	16	1.0250754216725824
1104	17	0.8104002618103507
1104	18	0.9715847162888167
1104	19	1.1884562579122715
1104	20	1.2904319504299622
1104	21	1.421693756645226
1104	22	0.31238901177444234
1104	23	0.558311750669553
1104	24	1.2636586641529703
1104	25	1.5053073033749698
1104	26	0.9844780836124158
1104	27	1.0713543579539788
1104	28	1.3451584328013837
1104	29	1.133612010652449
1104	30	1.1056844112051962
1104	31	1.6343956217463287
1104	32	1.5555530488913192
1104	33	1.7979462517737304
1104	34	1.3323909352162921
1104	35	0.9459939525571599
1104	36	1.149196721760127
1104	37	1.0505281560462514
1104	38	1.1032794660917595
1104	39	0.7287914966724465
1104	40	1.4777500881447807
1104	41	1.5662120975640015
1104	42	1.536583362230182
1104	43	1.805979941679837
1104	44	1.3089615277719027
1104	45	1.4580601964204192
1104	46	1.5654106558258967
1104	47	1.2422576098191485
1104	48	1.1154569098144371
1104	49	0.7596641799991595
1104	50	0.7616610378619804
1105	1	2.3633971530258258
1105	2	2.1009099980688255
1105	3	2.250009302775908
1105	4	2.542944893524865
1105	5	2.536131561445643
1105	6	2.732364672034432
1105	7	2.9101827118738335
1105	8	2.999869518221754
1105	9	3.214148443218207
1105	10	3.6416942814427387
1105	11	3.936936570672266
1105	12	3.8940261655948234
1105	13	3.146472913576119
1105	14	3.7101106203758363
1105	15	3.5987077765905795
1105	16	3.798788565100871
1105	17	3.735437743069717
1105	18	3.872084466413753
1105	19	3.5493258231296636
1105	20	4.098777777516418
1105	21	3.6292399835317894
1105	22	3.2811046539533493
1105	23	3.829776018535618
1105	24	3.475952517226439
1105	25	3.9682258441046088
1105	26	4.1877264594245105
1105	27	3.2445677512573283
1105	28	3.957902060987294
1105	29	3.8600487922616438
1105	30	4.230571967427089
1105	31	3.479373132990709
1105	32	3.1323646430942205
1105	33	3.4308797850071144
1105	34	3.358377941713041
1105	35	4.098867515775545
1105	36	3.883029805218392
1105	37	4.049328755746398
1105	38	3.9824898608943577
1105	39	3.359326229306131
1105	40	3.4698040611582783
1105	41	3.703093656784393
1105	42	3.540881213304644
1105	43	3.773246308496425
1105	44	3.0645837166774506
1105	45	3.0584599965203694
1105	46	2.8089388917079567
1105	47	2.570243616815649
1105	48	2.8881705529928468
1105	49	2.5174852694544327
1105	50	2.4596120623497377
1106	1	2.200856840448303
1106	2	2.235219102509408
1106	3	1.8953082360430855
1106	4	2.04455739662583
1106	5	2.6515867834749187
1106	6	2.8828484229647238
1106	7	2.5111750214744326
1106	8	2.6959250875593916
1106	9	2.4673858532901463
1106	10	1.876822041303818
1106	11	2.7429896592358958
1106	12	2.9671593831587018
1106	13	2.946472913576116
1106	14	3.3660768818494162
1106	15	3.2307097612097806
1106	16	2.779636989809376
1106	17	3.1435250663145737
1106	18	1.4480209585993151
1106	19	2.8198294202519634
1106	20	2.6616653984541543
1106	21	2.849334252943841
1106	22	2.691077365439334
1106	23	1.3685505161788853
1106	24	2.2048535343437763
1106	25	2.5177669009143315
1106	26	2.8061338025155536
1106	27	2.3962422737083315
1106	28	2.3737790145968205
1106	29	2.5532762792375863
1106	30	2.5487560408579952
1106	31	2.4992689404826436
1106	32	2.500709966835224
1106	33	2.5048068502514695
1106	34	2.495564743995356
1106	35	2.5515095400871317
1106	36	2.6747936355334545
1106	37	2.663915706875155
1106	38	2.5806540881332545
1106	39	2.7415638874554737
1106	40	2.4560109577099993
1106	41	2.7603003052587134
1106	42	3.0706256935824925
1106	43	2.9357370055939214
1106	44	2.5942289659952387
1106	45	2.929856672283208
1106	46	3.2820721092718337
1106	47	2.544989337480498
1106	48	3.0826136192295124
1106	49	3.1110352570505597
1106	50	3.128776041511234
1107	1	1.1423372131970808
1107	2	1.250042143405551
1107	3	2.034364776045983
1107	4	1.828295506808825
1107	5	1.2383764121746381
1107	6	1.6186409969592184
1107	7	1.6527423045607144
1107	8	1.6586984929961126
1107	9	2.007437033822228
1107	10	2.0614582249743307
1107	11	1.7286977370689343
1107	12	1.6046949922553182
1107	13	2.053855463911688
1107	14	1.8793307083184416
1107	15	1.9304828286618658
1107	16	1.7883280235595578
1107	17	1.8865601684342153
1107	18	2.4713901826831375
1107	19	1.9895942795055035
1107	20	2.2207402861949674
1107	21	2.279830124702812
1107	22	2.3326205067652026
1107	23	2.382842438345847
1107	24	2.0226315267149815
1107	25	1.760681294231894
1107	26	1.865763950745965
1107	27	2.029432365929857
1107	28	1.9610031949840518
1107	29	1.7085560822408183
1107	30	2.7664664363000355
1107	31	2.939035248241808
1107	32	3.012901555845893
1107	33	2.561636554273484
1107	34	1.852476530002786
1107	35	1.7770901494251632
1107	36	1.9252885416220025
1107	37	2.250995036699887
1107	38	2.3107009231484703
1107	39	2.215563211486181
1107	40	1.966078071803956
1107	41	2.604875651692975
1107	42	2.681219098053546
1107	43	2.8757688727176713
1107	44	3.095363628734841
1107	45	2.749179737321114
1107	46	2.416252013670462
1107	47	2.925994600384268
1107	48	2.456279786966462
1107	49	2.452592883432679
1107	50	2.6295727196049974
1108	1	0.8478686427711501
1108	2	1.1887621504240187
1108	3	0.313655910844421
1108	4	1.1771571991367686
1108	5	1.1195888524080075
1108	6	0.5631512395349993
1108	7	0.6898085852116758
1108	8	0.930137717455473
1108	9	0.5958477896234342
1108	10	0.43312993616439854
1108	11	0.8516086463991073
1108	12	0.37624388026689815
1108	13	0.8549696456022602
1108	14	0.9808791576758082
1108	15	0.44752454467712965
1108	16	0.17710724889622753
1108	17	0.474225212080313
1108	18	0.8955688026769977
1108	19	0.7271036009074408
1108	20	0.36900540879882016
1108	21	0.43235019313286926
1108	22	0.3552989293646647
1108	23	0.6847681303228654
1108	24	0.6387652285178476
1108	25	0.805818812326379
1108	26	0.6913550372924391
1108	27	0.45299687003127076
1108	28	0.6371447925626796
1108	29	0.8721741021557179
1108	30	0.6142095603953095
1108	31	0.8583228738065785
1108	32	0.7820378457085937
1108	33	0.999281858480181
1108	34	0.9743346692316344
1108	35	1.0211573512499683
1108	36	1.011089304880329
1108	37	0.44865262322442945
1108	38	1.2557375507749065
1108	39	1.2819315932908033
1108	40	1.109605728951827
1108	41	0.9627736207239899
1108	42	0.9514739561489094
1108	43	0.4827347015548007
1108	44	0.9611354408153794
1108	45	0.9985546550734483
1108	46	1.1290651031120582
1108	47	1.1454971665113902
1108	48	0.9097166426930947
1108	49	0.84860990321598
1108	50	0.7841105973959372
1109	1	0.2539143944051361
1109	2	0.1429245171250848
1109	3	0.20409278734940983
1109	4	0.4880447120366611
1109	5	-0.028931959425499088
1109	6	-0.0564738034646588
1109	7	-0.28473561843469497
1109	8	-0.49164590827552246
1109	9	-0.2819041883607447
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2211	12	-0.3424208007953986
2211	13	-0.8066916433859106
2211	14	-0.13201072401525948
2211	15	-0.2768487489441185
2211	16	0.15596883572504794
2211	17	-0.0751253298242176
2211	18	0.03166543542292288
2211	19	-0.05700329079439115
2211	20	-0.051156748891258985
2211	21	0.015621476329691575
2211	22	0.22855118385295725
2211	23	0.20681661216285363
2211	24	0.20366967130762248
2211	25	0.04731794362446351
2211	26	0.023736935199281106
2211	27	0.034572116163481326
2211	28	0.165191454265333
2211	29	0.2074516730997118
2211	30	0.05972954053926571
2211	31	-0.002643453652680705
2211	32	0.06527603549928074
2211	33	-0.13830397754199097
2211	34	-0.024424328038158194
2211	35	-0.07088368457364425
2211	36	0.47299052106300365
2211	37	0.5329167086054163
2211	38	0.28746585391051127
2211	39	0.44086267627251985
2211	40	0.44968184378594955
2211	41	0.20902992915453567
2211	42	0.4646699518467656
2211	43	1.0004396738390753
2211	44	0.6067050610685456
2211	45	0.885871607170742
2211	46	0.8838843303810258
2211	47	0.5181702863922517
2211	48	0.9996066071176841
2211	49	1.0812565483719325
2211	50	1.0121655324501262
2212	1	0.2827605482512894
2212	2	0.38044138622090173
2212	3	-0.5199288050932331
2212	4	0.10174241783963112
2212	5	0.2075727639213305
2212	6	0.16923469855963447
2212	7	0.4615531804856836
2212	8	0.43868472600248154
2212	9	0.07825100732076606
2212	10	-0.010560360467074759
2212	11	0.359527198435309
2212	12	0.026696368954681304
2212	13	0.08108829819150287
2212	14	-0.14028725760825012
2212	15	-0.2413894824721936
2212	16	-0.62291788936421
2212	17	0.018594241743457474
2212	18	0.20263265385849394
2212	19	-0.19811007430623562
2212	20	-0.18504185113079785
2212	21	-0.15550802707829092
2212	22	0.05904453016908917
2212	23	0.14303861800511442
2212	24	0.08333536426445676
2212	25	0.10257597673059848
2212	26	-0.3374282709026346
2212	27	-0.3012766342422353
2212	28	-0.5291448001974999
2212	29	-0.4625418355013977
2212	30	-0.4804359901325945
2212	31	-0.6738540995468689
2212	32	-1.144558433828859
2212	33	-0.7753536367435494
2212	34	-1.0826034906477027
2212	35	-0.5604163038530992
2212	36	-1.1966784175932759
2212	37	-2.406550996685077
2212	38	-1.3685711470632
2212	39	-0.9125128511536431
2212	40	-1.1080916063925663
2212	41	-1.7824501097938565
2212	42	-1.3469171951834191
2212	43	-0.9141984306724495
2212	44	-0.974761995082055
2212	45	-1.0006911971135821
2212	46	-1.1583909049321868
2212	47	-0.8254681426892141
2212	48	-0.7446322763617061
2212	49	-0.9702689303687357
2212	50	-0.452943198481389
2213	1	0.5991130699018257
2213	2	0.4673406900076209
2213	3	0.6295023377153903
2213	4	0.9288777549087435
2213	5	0.6550171517630687
2213	6	1.0839061198535695
2213	7	1.4827791950891829
2213	8	0.9220435305533385
2213	9	0.9744133438804568
2213	10	0.7298879329613541
2213	11	1.4995764427891842
2213	12	1.2836585017446325
2213	13	0.588857019536384
2213	14	-0.7612755408481888
2213	15	0.4341496582987361
2213	16	0.3594645185148906
2213	17	0.366403717036377
2213	18	0.5935733908257248
2213	19	0.5321072800833022
2213	20	0.37532493440155434
2213	21	0.31405386798029156
2213	22	0.39377878046625625
2213	23	-0.003930307109506259
2213	24	0.8380899915356395
2213	25	0.8597079176373725
2213	26	0.5919740045261364
2213	27	1.2969998133719614
2213	28	0.4889822162738895
2213	29	0.32124997974737113
2213	30	1.2754223898979689
2213	31	1.1696677207961415
2213	32	0.7138215967366008
2213	33	0.4489204335570136
2213	34	0.6824245276915768
2213	35	0.3908063145880618
2213	36	0.4323332991667854
2213	37	0.19219242325039687
2213	38	0.2265638503896774
2213	39	0.868392226109048
2213	40	0.5818387722795393
2213	41	0.3722875366656453
2213	42	0.2717786784793432
2213	43	0.44486083236032314
2213	44	0.755837427570345
2213	45	0.4483663678380232
2213	46	0.7707566583213392
2213	47	0.4544180858725113
2213	48	0.40454415427167945
2213	49	-0.1481004807168418
2213	50	-0.42127398469297006
2214	1	-0.4343767448353688
2214	2	-0.5401201197836407
2214	3	-0.17198982424286058
2214	4	0.44124258012460515
2214	5	-0.6723363564941209
2214	6	0.043626129913089784
2214	7	-1.0528901820589454
2214	8	-0.7182448423194927
2214	9	-1.3149654808324307
2214	10	-0.5164026194738902
2214	11	-0.38641567725440495
2214	12	-0.1145726995295746
2214	13	-0.19909670667705015
2214	14	-0.707960091103871
2214	15	-0.4287474831213345
2214	16	0.13698149395289505
2214	17	-0.4928468488115598
2214	18	-0.3290940582479607
2214	19	-0.21523113889565337
2214	20	-0.9688782678786012
2214	21	-1.0666570046829662
2214	22	-0.5562589427293219
2214	23	-0.4260947802422095
2214	24	-0.7963303286923775
2214	25	-0.6931883854894565
2214	26	-0.9825921787247687
2214	27	-0.8768202888998111
2214	28	-0.44873259636757723
2214	29	-0.30520655474839486
2214	30	-0.5731818518657974
2214	31	-0.38239028909571715
2214	32	-0.5233315594374268
2214	33	-0.9357723319723732
2214	34	-0.9041711634811982
2214	35	-0.3493646972318736
2214	36	-0.8308069472914248
2214	37	-0.897463038230029
2214	38	-0.6872176903932896
2214	39	-0.4768588427148224
2214	40	-0.39209030811278467
2214	41	-0.392235893630275
2214	42	-0.2631781494190548
2214	43	-0.44892741476852294
2214	44	-0.41861139462765706
2214	45	-0.6014701649811549
2214	46	-0.426242251897456
2214	47	-0.41853857436723985
2214	48	-0.7788744055405452
2214	49	-0.6719080085900906
2214	50	-0.7030243409675947
2215	1	-1.0773356055948646
2215	2	-1.5564175881380713
2215	3	-1.201499317913747
2215	4	-1.152744761647547
2215	5	-1.2614977488991848
2215	6	-0.7413422245172896
2215	7	-1.56072246053996
2215	8	-1.7337511714334148
2215	9	-1.1358515567817946
2215	10	-1.134598822005536
2215	11	-0.9574599810518727
2215	12	-1.546060041301729
2215	13	-1.428527086423884
2215	14	-1.5176911037620968
2215	15	-1.1551715337542454
2215	16	-0.7471166073129254
2215	17	-0.716501912102693
2215	18	-0.8537776025517623
2215	19	-1.4944241768703392
2215	20	-1.1581187742077184
2215	21	-1.3862772578475244
2215	22	-1.637429828805267
2215	23	-1.3934998435333448
2215	24	-1.6540043793252863
2215	25	-1.150308638654014
2215	26	-1.1678769888513507
2215	27	-2.1379753521909493
2215	28	-1.714080697633399
2215	29	-1.6955546560142167
2215	30	-1.7823590670556726
2215	31	-1.8044630739058434
2215	32	-1.6988853569057838
2215	33	-1.9109305598204713
2215	34	-1.6239496444938553
2215	35	-1.6435893807761772
2215	36	-1.6490822637471219
2215	37	-1.9948522787363565
2215	38	-1.3603980701401248
2215	39	-1.827096184486976
2215	40	-1.9502390422900007
2215	41	-1.4367770328707792
2215	42	-1.156532579798803
2215	43	-1.3390381742621926
2215	44	-0.9763645591846206
2215	45	-0.899729658652042
2215	46	-0.8748973151885941
2215	47	-1.3467822452533156
2215	48	-1.237260481489912
2215	49	-1.3997561098559146
2215	50	-1.671853454891643
2216	1	-0.8193614676638283
2216	2	-1.4818486226208307
2216	3	-1.205163111017196
2216	4	-1.8363654513027186
2216	5	-1.3828339557957356
2216	6	-1.3141870521034953
2216	7	-0.6018862536434071
2216	8	-1.1139235852265195
2216	9	-0.8824032809197284
2216	10	-1.4031333047641539
2216	11	-1.371684118982909
2216	12	-1.0042496964741403
2216	13	-2.0104236381480227
2216	14	-0.9088548968655417
2216	15	-1.1806025682370027
2216	16	-1.1512114348991318
2216	17	-0.7214588086544182
2216	18	-1.0365362232414164
2216	19	-0.6196396941117186
2216	20	-0.6684636017939241
2216	21	-0.6190358785371828
2216	22	-0.636136725356991
2216	23	-2.5598791538781747
2216	24	-1.453357827601149
2216	25	-1.504187948998844
2216	26	-1.6243425060927308
2216	27	-1.9588805246047443
2216	28	-1.3403738010816753
2216	29	-1.0675374146349057
2216	30	-1.5487383774005004
2216	31	-0.837868246319637
2216	32	-1.4555663913885404
2216	33	-1.6811891805101276
2216	34	-2.21058757552834
2216	35	-1.5597531738796242
2216	36	-1.5686943327126386
2216	37	-1.1282574511501515
2216	38	-1.705441173588401
2216	39	-1.9613634258662849
2216	40	-1.7595062836693103
2216	41	-1.7917339294225023
2216	42	-1.7746360280746636
2216	43	-2.1922709328828844
2216	44	-1.849209386770827
2216	45	-2.3674020724451452
2216	46	-2.0686473151885956
2216	47	-2.393549486632626
2216	48	-1.8635535849381881
2216	49	-2.313549213304192
2216	50	-2.8300431100640573
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7446.0
3	296.0
4	601.0
5	400.0
6	322.0
7	261.0
8	272.0
9	236.0
10	272.0
11	374.0
12	433.0
13	483.0
14	464.0
15	379.0
16	339.0
17	315.0
18	301.0
19	354.0
20	545.0
21	793.0
22	1113.0
23	1374.0
24	1322.0
25	1171.0
26	999.0
27	1017.0
28	1140.0
29	1180.0
30	1317.0
31	1602.0
32	2104.0
33	2708.0
34	3297.0
35	5020.0
36	6428.0
37	7880.0
38	12317.0
39	27729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.123903821945774	24.84529212021199	15.490886780277787	20.53991727756445
2	14.002200406228843	27.60557718348003	37.68618821936357	20.706034190927554
3	15.72288455638891	33.66832044514498	30.43763421523098	20.171160783235127
4	11.208219149093757	23.486155050734837	39.73188306123097	25.573742738940442
5	10.148132472754206	43.24727542059041	34.30430642260078	12.300285684054597
6	23.216476960182796	45.88181779714805	17.616256928870648	13.2854483137985
7	21.98271759019324	38.900229515479076	24.117105778078628	14.99994711624906
8	20.826149045327128	39.75247262918496	21.437562807425824	17.983815518062094
9	23.015411303032547	23.041854856623054	21.91430173152389	32.02843210882051
10	14.176143877281142	33.420788151282736	33.23142025919069	19.171647712245438
11	24.84636295363916	33.61610306639447	25.185898181741255	16.351635798225107
12	18.9824806398375	35.84803859337311	28.11243705302357	17.057043713765815
13	23.728221890768303	30.29100948874996	27.164059111632977	18.81670950884876
14	18.758264659522474	31.512022765500536	28.7657755820965	20.96393699288049
15	20.273387079709686	37.68700141771937	23.989081444804164	18.050530057766775
16	18.44187719520968	35.80783716304854	27.77707248952647	17.973213152215312
17	16.197227806581314	37.13046238493281	27.681726801396678	18.990583007089196
18	16.032416076850648	35.613249965616106	30.01237820166947	18.341955755863776
19	18.164601826087875	34.69249568869751	31.0371459706514	16.10575651456321
20	16.793820432781335	35.162160732236394	30.64176498597958	17.4022538490027
21	18.493476811732215	34.03908622459237	30.16855537567851	17.298881587996913
22	18.78749404856372	32.42871501878008	30.687192509125538	18.096598423530658
23	18.259627592044012	34.80321625052899	30.028565382987725	16.908590774439272
24	18.15373874779187	34.758877476543574	29.550334789553297	17.53704898611126
25	18.60743154598163	33.484277794235894	30.565195310846843	17.34309534893563
26	17.465557742365565	33.9988995407699	31.6551330074281	16.88040970943644
27	16.858878049812727	34.65412531476818	30.208223120384282	18.27877351503481
28	18.020382462192966	32.78443905897792	31.910300233879756	17.28487824494936
29	17.2252145479942	34.464185564173924	30.689622332038812	17.62097755579306
30	17.651664233267738	34.35324488457225	29.757294906790243	18.23779597536977
31	19.36432697801384	32.241784285925895	31.065239012209833	17.328649723850432
32	16.762773375091257	34.465100039148055	31.2369726915874	17.535153894173288
33	17.888165899592657	34.39559858223563	29.93598899645559	17.78024652171613
34	18.677273304277918	32.090720602547286	31.353404138281217	17.87860195489358
35	17.644320297951584	34.97333671914677	30.20568816658202	17.17665481631962
36	17.97557827016274	33.501576619473894	29.19708801557573	19.325757094787633
37	19.042226690655095	31.94306275796381	31.13239496242989	17.88231558895121
38	17.514178093787034	33.935373285931945	31.262696800406296	17.287751819874725
39	16.67847812060376	34.38719708909362	30.21546207465544	18.71886271564718
40	17.562621646780062	32.91867648303292	32.200431581619696	17.31827028856732
41	17.406572948730155	33.71624408974074	31.01682903352056	17.860353928008546
42	17.54818372225866	33.825925063998135	29.956417796771532	18.66947341697167
43	17.946657078195393	32.612787918526195	30.508259480953487	18.93229552232492
44	17.119832481994987	33.725689266791456	30.733843078777877	18.420635172435674
45	18.040484802335175	32.63108911309939	29.82634261903251	19.502083465532923
46	18.187106053555567	32.51300816447396	30.381995854308556	18.917889927661914
47	18.560974578063533	33.677720908591006	29.711095131339622	18.05020938200584
48	17.176791606734916	33.90197986293257	29.099331584736444	19.821896945596073
49	18.928552541117984	32.17409699085092	30.341107409170238	18.55624305886086
50	17.237221993083537	32.3688357287139	30.460991782734226	19.93295049546834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2263.0
1	1449.5
2	636.0
3	647.5
4	659.0
5	551.0
6	443.0
7	385.5
8	328.0
9	323.5
10	319.0
11	375.5
12	432.0
13	650.0
14	868.0
15	1137.5
16	1407.0
17	1487.0
18	1567.0
19	1442.5
20	1318.0
21	1290.5
22	1263.0
23	1474.5
24	1686.0
25	2142.0
26	2598.0
27	3020.5
28	3443.0
29	3953.5
30	4464.0
31	5000.0
32	5536.0
33	6540.0
34	7544.0
35	7393.5
36	7243.0
37	7551.0
38	7859.0
39	7852.0
40	7845.0
41	7493.0
42	7141.0
43	6163.5
44	5186.0
45	4944.0
46	4702.0
47	4741.0
48	4780.0
49	4194.5
50	3609.0
51	3021.0
52	2433.0
53	2125.0
54	1817.0
55	1583.5
56	1350.0
57	1161.0
58	972.0
59	840.0
60	708.0
61	636.0
62	564.0
63	475.0
64	386.0
65	336.5
66	287.0
67	260.5
68	234.0
69	205.5
70	177.0
71	154.5
72	132.0
73	121.5
74	111.0
75	96.5
76	82.0
77	73.0
78	64.0
79	56.0
80	48.0
81	36.5
82	25.0
83	21.0
84	17.0
85	16.0
86	15.0
87	10.0
88	5.0
89	5.0
90	5.0
91	5.0
92	5.0
93	5.5
94	6.0
95	5.5
96	5.0
97	7.0
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07504968077459727
2	0.08033486956154073
3	0.07716375628937465
4	0.09830451143714852
5	0.09936154919453723
6	0.07610671853198596
7	0.06025115217115555
8	0.07293560525981987
9	0.06659337871548772
10	0.08350598283370682
11	0.06659337871548772
12	0.08456302059109552
13	0.07504968077459727
14	0.07927783180415204
15	0.09090524713542768
16	0.08456302059109552
17	0.09936154919453723
18	0.08773413386326159
19	0.08984820937803899
20	0.10464673798148069
21	0.10041858695192592
22	0.09407636040759376
23	0.08879117162065028
24	0.0708215297450425
25	0.09301932265020507
26	0.10358970022409199
27	0.09513339816498245
28	0.11733119107014503
29	0.10887488901103548
30	0.09090524713542768
31	0.09513339816498245
32	0.09619043592237116
33	0.09407636040759376
34	0.07610671853198596
35	0.09724747367975983
36	0.10358970022409199
37	0.1205023043423111
38	0.09724747367975983
39	0.06659337871548772
40	0.07187856750243118
41	0.0687074542302651
42	0.07399264301720858
43	0.048623736839879916
44	0.049680774597268614
45	0.0528518878694347
46	0.05073781235465731
47	0.04228151029554776
48	0.05496596338421208
49	0.062365227685932945
50	0.049680774597268614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	94604.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.134159232167775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.50153078810243	44.05416261468859
2	6.110987206261025	6.371823601539046
3	2.485756574278705	3.887784871675616
4	1.4395490764583039	3.001987230983891
5	0.9651061414002149	2.515749862585092
6	0.7339672756026845	2.2958860090482434
7	0.5312138845522192	1.9386072470508646
8	0.41767198556395857	1.7419982241765677
9	0.36495610389083755	1.7124011669696841
>10	2.2830031832282396	21.289797471565684
>50	0.13178970418280245	4.4363874677603485
>100	0.028385474747065147	3.2324214620946266
>500	0.004055067821009306	1.3847194621791892
>1k	0.002027533910504653	2.13627330768255
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2021	2.13627330768255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.6934167688469832	No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA	654	0.6913026933322058	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	493	0.5211196143926261	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	455	0.4809521796118557	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.3467083844234916	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	240	0.25368906177328654	No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC	239	0.25263202401589785	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	206	0.21774977802207096	No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	191	0.20189421166124052	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.19660902287429705	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	160	0.16912604118219104	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124	0.13107268191619806	No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	117	0.12367341761447719	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	115	0.12155934209969979	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	103	0.10887488901103548	No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG	101	0.10676081349625809	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	98	0.10358970022409199	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	98	0.10358970022409199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.001057037757388694	0.0
13	0.0	0.0	0.0	0.001057037757388694	0.0
14	0.0	0.0	0.0	0.001057037757388694	0.0
15	0.0	0.0	0.0	0.001057037757388694	0.0
16	0.0	0.0	0.0	0.001057037757388694	0.0
17	0.0	0.0	0.0	0.001057037757388694	0.0
18	0.0	0.0	0.0	0.001057037757388694	0.0
19	0.0	0.0	0.0	0.001057037757388694	0.0
20	0.0	0.0	0.0	0.001057037757388694	0.0
21	0.0	0.0	0.0	0.002114075514777388	0.0
22	0.0	0.0	0.0	0.006342226544332164	0.0
23	0.0	0.0	0.0	0.012684453088664328	0.0
24	0.0	0.0	0.0	0.023254830662551268	0.0
25	0.0	0.0	0.0	0.023254830662551268	0.0
26	0.0	0.0	0.0	0.02959705720688343	0.0
27	0.0	0.0	0.0	0.03171113272166082	0.0
28	0.0	0.0	0.0	0.041224472538159064	0.0
29	0.0	0.0	0.0	0.056023001141600776	0.0
30	0.0	0.0	0.0	0.08350598283370682	0.0
31	0.0	0.0	0.0	0.11310304004059026	0.0
32	0.0	0.0	0.0	0.14481417276225106	0.0
33	0.0	0.0	0.0	0.18075345651346666	0.0
34	0.0	0.0	0.0	0.22937719335334658	0.0
35	0.0	0.0	0.0	0.28434315673755867	0.0
36	0.0	0.0	0.0	0.34248023339393685	0.0
37	0.0	0.0	0.0	0.400617310050315	0.0
38	0.0	0.0	0.0	0.4703818020379688	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCACAT	25	0.0023471264	35.183914	42
AGTTTTA	35	3.194273E-4	31.430841	16
TAAGTCC	30	0.005716567	29.335451	21
ATATAGT	30	0.005716567	29.335451	4
AGGGGTC	30	0.005716567	29.335451	13
CCGCGAT	30	0.005716567	29.335451	11
ACCCTGG	30	0.0057313866	29.319927	6
ACCTGAT	40	6.959376E-4	27.516552	35
TGAACTT	45	0.001388121	24.446207	10
ATAAGGC	45	0.001388121	24.446207	3
TAAGGCT	55	1.5777329E-4	24.001734	4
CATGGGG	95	2.6175258E-8	23.159567	4
CCTGATG	50	0.0025540798	22.01324	36
TTTAAGT	70	3.1733136E-5	22.001589	19
TATAAGG	50	0.002561955	22.001589	2
CTATGAT	50	0.0025698503	21.989946	9
CTTTCCA	50	0.0025698503	21.989946	9
GGGATGG	50	0.0025698503	21.989946	5
AAGGCTA	60	2.854547E-4	21.989944	5
GTACATG	245	0.0	20.654554	1
>>END_MODULE