##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077433_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94604 Sequences flagged as poor quality 0 Sequence length 50 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.601581328485054 31.0 30.0 34.0 25.0 34.0 2 29.594330049469367 31.0 30.0 34.0 25.0 34.0 3 29.69955815821741 31.0 30.0 34.0 25.0 34.0 4 33.10072512790157 37.0 35.0 37.0 27.0 37.0 5 32.702919538285904 37.0 33.0 37.0 25.0 37.0 6 32.57967950615196 37.0 33.0 37.0 23.0 37.0 7 32.372870068918864 37.0 33.0 37.0 19.0 37.0 8 32.31355968035178 37.0 33.0 37.0 19.0 37.0 9 33.58430933152932 39.0 34.0 39.0 17.0 39.0 10 33.50737812354657 39.0 34.0 39.0 17.0 39.0 11 33.443237072428225 39.0 34.0 39.0 17.0 39.0 12 33.41092342818485 39.0 34.0 39.0 17.0 39.0 13 33.31155130861274 38.0 33.0 39.0 17.0 39.0 14 34.33699420743309 39.0 34.0 41.0 17.0 41.0 15 34.35646484292419 39.0 34.0 41.0 17.0 41.0 16 34.31845376516849 39.0 34.0 41.0 17.0 41.0 17 34.3307154031542 39.0 34.0 41.0 17.0 41.0 18 34.322766479218636 39.0 34.0 41.0 17.0 41.0 19 34.42336476258932 39.0 34.0 41.0 18.0 41.0 20 34.446883852691215 39.0 34.0 41.0 18.0 41.0 21 34.36669696841571 39.0 34.0 41.0 18.0 41.0 22 34.350281172043466 39.0 34.0 41.0 18.0 41.0 23 34.25304426874128 39.0 34.0 41.0 18.0 41.0 24 34.1866094456894 39.0 34.0 41.0 17.0 41.0 25 34.14627288486745 39.0 34.0 41.0 16.0 41.0 26 34.047249587755275 39.0 34.0 41.0 16.0 41.0 27 33.936789142108154 39.0 33.0 41.0 13.0 41.0 28 33.854287345143966 39.0 33.0 41.0 10.0 41.0 29 33.73501120460023 39.0 33.0 41.0 10.0 41.0 30 33.51859329415247 39.0 33.0 41.0 9.0 41.0 31 33.29742928417403 38.0 33.0 41.0 8.0 41.0 32 33.12938142150438 38.0 33.0 41.0 2.0 41.0 33 32.89927487209843 38.0 33.0 41.0 2.0 41.0 34 32.83891801615154 38.0 33.0 41.0 2.0 41.0 35 32.721238002621455 38.0 33.0 41.0 2.0 41.0 36 32.5291848124815 38.0 32.0 41.0 2.0 41.0 37 32.44852226121517 38.0 32.0 41.0 2.0 41.0 38 32.26483023973616 38.0 32.0 40.0 2.0 41.0 39 32.165162149591985 38.0 32.0 40.0 2.0 41.0 40 32.057703691175846 38.0 31.0 40.0 2.0 41.0 41 31.977284258593716 38.0 31.0 40.0 2.0 41.0 42 31.822597353177457 38.0 31.0 40.0 2.0 41.0 43 31.773561371612193 38.0 31.0 40.0 2.0 41.0 44 31.614012092511945 38.0 31.0 41.0 2.0 41.0 45 31.446302481924654 38.0 30.0 41.0 2.0 41.0 46 31.18937888461376 38.0 30.0 41.0 2.0 41.0 47 30.921504376136316 38.0 30.0 41.0 2.0 41.0 48 30.66634603188026 38.0 30.0 41.0 2.0 41.0 49 30.479810578833877 38.0 29.0 40.0 2.0 41.0 50 30.337744704240837 38.0 29.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.4231644404766115 1101 2 -0.2965278107970519 1101 3 -0.38469565994189026 1101 4 -0.6755519566536634 1101 5 -0.35478586966003434 1101 6 -0.09300919857268042 1101 7 0.009480881551475306 1101 8 -0.33169811127768156 1101 9 0.0931254447491412 1101 10 -0.2727892830640215 1101 11 -0.26947906617149187 1101 12 -0.21551254386087493 1101 13 -0.07566147041490723 1101 14 -0.01573796384844428 1101 15 -0.10052827116489738 1101 16 -0.151644850729312 1101 17 0.4804850715199507 1101 18 0.18675675829037885 1101 19 0.4560328757758896 1101 20 0.12989995472242555 1101 21 0.2686024440075627 1101 22 0.38298403152526816 1101 23 0.03719910022075368 1101 24 0.04946958678898028 1101 25 0.03625608483169884 1101 26 -0.4308490264899021 1101 27 -0.023764758339680725 1101 28 0.18345227207402814 1101 29 -0.1759133199627172 1101 30 0.09045329747306852 1101 31 0.4078535069753215 1101 32 0.04690403764430329 1101 33 -0.20450193743349843 1101 34 -0.24108085791018397 1101 35 -0.20885793398844754 1101 36 -0.49163476759530056 1101 37 -0.8667011241870206 1101 38 -0.33349052186980543 1101 39 -0.16115539704981785 1101 40 -0.5564907483783124 1101 41 -0.6003343682768758 1101 42 -0.030719917059943924 1101 43 0.3735213974885738 1101 44 0.20851478210422414 1101 45 0.5850582607332626 1101 46 0.4945558347596304 1101 47 0.6323333642022568 1101 48 0.0854919367018745 1101 49 -0.05181562342907853 1101 50 -0.11553151213248825 1102 1 1.7216968133774486 1102 2 1.7416530730672868 1102 3 1.7972159536244021 1102 4 1.8455169025446878 1102 5 1.821062023454047 1102 6 1.4217903903276294 1102 7 1.9403647458885729 1102 8 2.20093292674904 1102 9 1.983310439610392 1102 10 2.283068887542349 1102 11 1.8327819821327225 1102 12 2.3329972197568054 1102 13 2.5016252716200356 1102 14 2.4540462088134305 1102 15 3.1026063639841226 1102 16 2.6335555603703753 1102 17 2.256076027326735 1102 18 2.312604651404463 1102 19 2.5989927935559507 1102 20 2.673836234270219 1102 21 2.55411117987601 1102 22 2.6762522740740877 1102 23 2.751536201967923 1102 24 2.022748106830093 1102 25 2.081322888531595 1102 26 2.106920757682758 1102 27 2.2640050258329794 1102 28 2.0474810490524433 1102 29 1.9726478311164186 1102 30 2.001758538994139 1102 31 2.010525654971211 1102 32 2.228250389134544 1102 33 2.22561111498171 1102 34 2.246020889263505 1102 35 1.8102582016744506 1102 36 1.7615512550151848 1102 37 1.908530119389546 1102 38 1.6748342468866184 1102 39 1.8401806769481297 1102 40 1.865297535280476 1102 41 1.906859876327438 1102 42 1.5725688804734546 1102 43 1.7852891261627732 1102 44 1.1218241346222122 1102 45 1.5067437694691321 1102 46 1.5210203774112223 1102 47 1.6517064371724537 1102 48 1.8616995420461677 1102 49 1.4343097619768805 1102 50 1.2972125783094448 1103 1 0.9692902878936103 1103 2 1.445625106707979 1103 3 0.9774773782874853 1103 4 1.0578484457608184 1103 5 0.8924978325532464 1103 6 1.8498479442844626 1103 7 1.7433843020746096 1103 8 1.477658025543242 1103 9 2.0827632717109097 1103 10 2.0896855660305462 1103 11 1.326094374472369 1103 12 1.0979683414510006 1103 13 1.7800729360237284 1103 14 1.7758113319262065 1103 15 2.0017856121349027 1103 16 1.888596597962838 1103 17 1.884609820010894 1103 18 1.4755897562783105 1103 19 1.6620690930797082 1103 20 1.8889542806781634 1103 21 1.9350008343496157 1103 22 1.9003884580846275 1103 23 2.117581393983329 1103 24 2.3271355827648392 1103 25 2.1311253001234505 1103 26 1.9670382974537617 1103 27 1.7438408754631354 1103 28 1.8299311453134663 1103 29 2.371269778628836 1103 30 2.1936726133684417 1103 31 2.0730446538566056 1103 32 2.2482118847268 1103 33 2.101333072626865 1103 34 2.1081143131159763 1103 35 1.7889287863334857 1103 36 1.561986962786996 1103 37 2.101508131346179 1103 38 2.4459863392971926 1103 39 2.364397922869699 1103 40 1.8642335976981457 1103 41 1.9771304204555165 1103 42 1.7952358173464944 1103 43 1.5251310554362547 1103 44 1.6740077398507047 1103 45 1.8862655041940286 1103 46 1.9075337019628513 1103 47 1.9676452354296181 1103 48 2.016578455816564 1103 49 1.447178340353613 1103 50 1.8299413248896457 1104 1 0.8976430129395361 1104 2 0.9832858537992202 1104 3 0.670169764488751 1104 4 0.5335577741951951 1104 5 0.9125019150190568 1104 6 0.7049204080525797 1104 7 0.812275912607376 1104 8 0.06012383489208872 1104 9 -0.2730236235738417 1104 10 0.20930181424446914 1104 11 0.8278294224413401 1104 12 0.8387317625573445 1104 13 0.26843790846708515 1104 14 0.3871605868761989 1104 15 0.417380631526612 1104 16 0.31419365858745607 1104 17 0.33276250423216425 1104 18 0.4112909197810666 1104 19 1.1643144492748547 1104 20 0.8995686917824699 1104 21 0.8510041003222639 1104 22 0.7418867230366573 1104 23 1.2443419573636092 1104 24 1.2420161706399142 1104 25 1.1922602256184476 1104 26 1.5582814082927072 1104 27 1.3385540822400301 1104 28 1.0254812575812764 1104 29 0.6523475496025029 1104 30 1.4480102332908729 1104 31 0.8538162398945772 1104 32 0.7528046587623152 1104 33 1.430073631924678 1104 34 1.066269038570148 1104 35 1.0180571798831863 1104 36 0.5981167851997284 1104 37 0.9341270331628735 1104 38 0.9224100992482036 1104 39 1.1531303172358989 1104 40 1.4960972433401096 1104 41 1.2837236027376164 1104 42 1.3947459214493705 1104 43 1.253749140759794 1104 44 1.222282898046256 1104 45 1.7243957348533456 1104 46 1.5841003482171878 1104 47 1.8017950619248246 1104 48 1.4969826199316856 1104 49 0.5941471094901729 1104 50 0.947822525134594 1105 1 1.3928454117401365 1105 2 1.451501745853193 1105 3 1.5778659164127902 1105 4 1.7027731664990426 1105 5 1.9540811254138575 1105 6 1.7222617373879103 1105 7 1.713175462832261 1105 8 1.6601738099045846 1105 9 2.088445641793477 1105 10 2.449131899201987 1105 11 2.764511081611758 1105 12 2.3899061753509514 1105 13 2.1711365591417433 1105 14 2.6566758292550077 1105 15 2.4706539948449517 1105 16 2.5330842133100973 1105 17 2.2664456626529557 1105 18 2.411140994743583 1105 19 2.3777077526231807 1105 20 2.2882743389588853 1105 21 2.444957123810518 1105 22 2.0186983172395543 1105 23 2.1719281642601587 1105 24 2.2120311631436635 1105 25 2.447832439511501 1105 26 2.2605302838549264 1105 27 2.3003731726948047 1105 28 2.4420729617292025 1105 29 2.49132805934763 1105 30 2.08799024328588 1105 31 2.388149073477784 1105 32 2.7914853184324855 1105 33 2.2235768803004916 1105 34 1.6035004228779925 1105 35 2.147792312316973 1105 36 1.7637839516165243 1105 37 1.8175353290149516 1105 38 1.7438493796080223 1105 39 1.6186475586152085 1105 40 2.1183860989123247 1105 41 2.2238035627576096 1105 42 1.643171708555819 1105 43 2.020565732463943 1105 44 1.9895492648628483 1105 45 2.5121518567923786 1105 46 2.243570613084753 1105 47 1.6912003592761486 1105 48 2.1650485869481813 1105 49 2.1767558051423457 1105 50 2.8440743991975594 1106 1 1.074214798988958 1106 2 1.4466890442903058 1106 3 0.9123238672934662 1106 4 0.9174593485878546 1106 5 1.180774253669874 1106 6 1.3354594550758279 1106 7 1.4007964005648361 1106 8 1.258486883583572 1106 9 1.566044252560033 1106 10 1.9233566573265222 1106 11 1.7608395360895557 1106 12 1.2250835506920552 1106 13 1.6095389406847858 1106 14 1.9432548419069562 1106 15 2.2392970114661424 1106 16 1.892548366125773 1106 17 2.06183142301019 1106 18 1.6507341478073414 1106 19 2.0162589806063096 1106 20 1.8033326371479177 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1.3507084083771588 1107 9 1.5520420315180772 1107 10 1.2146028549992565 1107 11 1.6638862262541991 1107 12 1.3469994407987613 1107 13 1.7485954806876833 1107 14 1.6909734455475345 1107 15 1.5429520739932983 1107 16 1.1417396171517993 1107 17 1.6196509392093077 1107 18 1.338194917446664 1107 19 1.0074230962936213 1107 20 1.4153745472439994 1107 21 1.717116346212741 1107 22 1.4844701293200941 1107 23 1.4289057157528688 1107 24 2.0389230863095946 1107 25 2.0001582900090753 1107 26 1.6312315104228503 1107 27 1.6742218188415308 1107 28 1.8888213995908032 1107 29 1.3217376867486905 1107 30 1.4190247260444977 1107 31 1.978706814741379 1107 32 2.215600106675943 1107 33 2.110454917791813 1107 34 1.9846621909872297 1107 35 2.2279589404123215 1107 36 2.3087966801515805 1107 37 2.208370783770796 1107 38 2.3281585672960645 1107 39 2.250445753477507 1107 40 2.1773539304328082 1107 41 2.2687931485016506 1107 42 2.1524056821730397 1107 43 2.1378071117742863 1107 44 2.526971157943148 1107 45 1.792920197454258 1107 46 2.218668969946595 1107 47 1.8388050804055176 1107 48 2.320151572368225 1107 49 2.6519235903772476 1107 50 2.372100319123504 1108 1 0.24504284243655405 1108 2 0.25253848238688903 1108 3 0.44723711316735404 1108 4 0.6736543925526846 1108 5 0.13699751035863983 1108 6 0.5524623233694328 1108 7 0.6689113203936756 1108 8 0.46918891019757325 1108 9 -0.04128165142266482 1108 10 0.13612761702935217 1108 11 0.6469541737911584 1108 12 0.8357479603408109 1108 13 0.9298473997998471 1108 14 0.7561574609881632 1108 15 0.402319534624084 1108 16 0.7904653266749833 1108 17 0.10471476339670005 1108 18 0.3932460206619979 1108 19 0.5192448272231331 1108 20 -0.21580499392369745 1108 21 0.15805155698608786 1108 22 -0.15907946053822286 1108 23 -0.020363924989332816 1108 24 0.2866311180681578 1108 25 0.3928854032257263 1108 26 0.08712482971925084 1108 27 0.17884962074528943 1108 28 -0.47863923119394514 1108 29 0.18715857546212078 1108 30 0.8046456410771796 1108 31 0.8259674192068829 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18 -0.49199782046419926 2212 19 -1.101293131550122 2212 20 -0.5028839280614577 2212 21 -0.7440349186298008 2212 22 -0.7787375801963421 2212 23 -0.8005298375081864 2212 24 -1.056757519438122 2212 25 -0.5741834756078603 2212 26 -0.6808490264899021 2212 27 -1.0750468096217318 2212 28 -1.0447528561311046 2212 29 -0.7297594738088691 2212 30 -1.2348214278016556 2212 31 -1.786651987530174 2212 32 -1.6666490759088077 2212 33 -1.451754684686243 2212 34 -0.9802749971043205 2212 35 -1.148052073182587 2212 36 -1.0498765258370604 2212 37 -1.2007670582529535 2212 38 -1.144479532858817 2212 39 -1.9878953237897434 2212 40 -1.249164741052308 2212 41 -0.940077958020467 2212 42 -1.5032473895874183 2212 43 -2.105782631815458 2212 44 -1.8761006025111584 2212 45 -2.4651248894498856 2212 46 -1.6840155938117967 2212 47 -2.009241727372835 2212 48 -1.6214677702578335 2212 49 -1.5014493230627792 2212 50 -1.3908062374072117 2213 1 -0.8436469381114264 2213 2 -1.4182402043637516 2213 3 -0.9154430582389566 2213 4 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2214 25 -0.974865072491987 2214 26 -1.0859123176291448 2214 27 -0.8465495684662656 2214 28 -0.4481932975462257 2214 29 -0.7373057250260047 2214 30 -0.6505955271200605 2214 31 -1.1800307606557823 2214 32 -1.4070742625365256 2214 33 -0.8699630585908196 2214 34 -0.26467941125557104 2214 35 -0.37865901897940546 2214 36 -0.16550456868029073 2214 37 -0.19346423449443506 2214 38 -0.19316502458228513 2214 39 0.03287715267893532 2214 40 0.1756973167211413 2214 41 -0.3091951277705469 2214 42 -0.19166024255723357 2214 43 0.26438939025529606 2214 44 0.34712237704093596 2214 45 0.034063685688053624 2214 46 -1.0004712900143282 2214 47 -0.6934351710599493 2214 48 -0.7250867251426101 2214 49 -0.4650163467563857 2214 50 -0.21480818482688235 2215 1 -0.18387872619089762 2215 2 -0.1411706679399103 2215 3 -0.5829099456561764 2215 4 -0.4728733852250926 2215 5 0.07110698748282118 2215 6 -0.3501520557155402 2215 7 -0.22087626130566917 2215 8 -0.42991239699196626 2215 9 -0.10508884096514493 2215 10 -0.630824997349734 2215 11 -0.1775147804572086 2215 12 -0.4458696867180123 2215 13 -1.249768613272046 2215 14 -1.1907379638484414 2215 15 -0.8487425568791878 2215 16 -0.8320019935864593 2215 17 -1.120407785622909 2215 18 -1.29985038456676 2215 19 -0.6466456956526869 2215 20 -0.6272429024204271 2215 21 -0.5546118417067234 2215 22 -0.6063016827604457 2215 23 -1.2869080426363908 2215 24 -1.3362446989253058 2215 25 -1.2592796294540136 2215 26 -1.4245990264899007 2215 27 -1.1862647583396821 2215 28 -0.9165477279259733 2215 29 -0.9946633199627186 2215 30 -1.2247252739554995 2215 31 -1.3010750644532507 2215 32 -1.5414888194985537 2215 33 -1.7589662231477838 2215 34 -1.535723715053038 2215 35 -1.7160007911313073 2215 36 -1.534491910452445 2215 37 -1.3175939813298783 2215 38 -1.0156333790126624 2215 39 -1.7343696827641004 2215 40 -1.578812176949743 2215 41 -1.550334368276875 2215 42 -1.5691127742028002 2215 43 -1.3996928882257151 2215 44 -1.4102352178957744 2215 45 -1.6354774535524506 2215 46 -1.4027655938117967 2215 47 -2.085523778654885 2215 48 -1.8573652061552686 2215 49 -1.8607441948576522 2215 50 -1.2994600835610584 2216 1 -2.964051139984001 2216 2 -2.215739633457151 2216 3 -2.2566168422078974 2216 4 -3.7075716610871616 2216 5 -3.41833266768959 2216 6 -3.5294624005431245 2216 7 -3.469583157857393 2216 8 -3.3415503280264502 2216 9 -3.108106082344456 2216 10 -3.8474198249359404 2216 11 -3.6820406425261716 2216 12 -3.3807834798214635 2216 13 -3.8357599925823926 2216 14 -4.014013825917406 2216 15 -4.060380487913669 2216 16 -4.389329579793355 2216 17 -4.5076922683815255 2216 18 -4.142307281118484 2216 19 -4.406775005997513 2216 20 -4.822070488627325 2216 21 -4.649870462396375 2216 22 -4.915784441381135 2216 23 -3.9453132150501844 2216 24 -5.175899871339098 2216 25 -4.745271008764359 2216 26 -4.06878006097266 2216 27 -3.9547992410983 2216 28 -4.442409796891489 2216 29 -4.196602975135129 2216 30 -4.555113204989986 2216 31 -4.148057823073941 2216 32 -3.979204336739933 2216 33 -4.395388636940886 2216 34 -4.568913370225452 2216 35 -4.038414584234754 2216 36 -3.856905703555892 2216 37 -3.5376370847781544 2216 38 -3.576840275564386 2216 39 -4.062386924143411 2216 40 -5.176441487294571 2216 41 -4.531368851035495 2216 42 -4.663724843168318 2216 43 -3.741503233053301 2216 44 -3.741485217895775 2216 45 -3.468882625966245 2216 46 -3.080567317949729 2216 47 -3.8363858476204022 2216 48 -3.41943417167251 2216 49 -3.226692470719719 2216 50 -3.245580773216229 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4002.0 3 580.0 4 1003.0 5 542.0 6 419.0 7 293.0 8 208.0 9 227.0 10 204.0 11 208.0 12 282.0 13 284.0 14 326.0 15 355.0 16 372.0 17 320.0 18 382.0 19 400.0 20 443.0 21 598.0 22 814.0 23 922.0 24 1033.0 25 1176.0 26 1190.0 27 1161.0 28 1464.0 29 1665.0 30 1864.0 31 2175.0 32 2605.0 33 3289.0 34 4009.0 35 5575.0 36 6604.0 37 7820.0 38 11215.0 39 28575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.51637013309653 24.15717864958295 17.937902381783008 22.388548835537513 2 12.726869126771877 24.98335147933998 39.73129816179191 22.558481232096234 3 14.208701534818823 31.2079827491438 33.70576296985328 20.877552746184094 4 10.126635800511616 21.85788883955942 39.865964778757316 28.149510581171644 5 9.40632798950975 41.236622816293725 37.0267755171101 12.330273677086417 6 23.213813667748724 43.36755733666057 19.205270003066413 14.213358992524295 7 20.744821460670593 36.42054286107028 25.882651496727394 16.951984181531728 8 21.708147427628933 36.08403290266647 24.06800448288258 18.139815186822016 9 22.30975014010341 18.08338532139194 24.318780201537436 35.28808433696721 10 14.274082690070847 30.54668499524162 35.34630432483875 19.832927989848788 11 25.41001808203534 28.61508528164621 27.636963486977763 18.337933149340692 12 18.707173673970054 31.929405295660267 30.99462820404365 18.36879282632603 13 25.229988368404356 25.204610341545948 30.693666067463255 18.871735222586445 14 18.512996214284204 27.13025823234566 32.50005287312565 21.856692680244485 15 20.44115936511965 32.86489230085969 27.364146813437806 19.329801520582855 16 18.81100172364567 30.402783211902673 31.14722894878763 19.638986115664025 17 15.40893805122565 33.264947864892875 31.66705441932277 19.659059664558704 18 15.949661590524537 29.110617597292727 34.879441624365484 20.060279187817258 19 17.587089405444278 29.56598066794983 35.97157300281309 16.875356923792804 20 15.611649253415678 29.651875978173514 35.92487627426928 18.811598494141535 21 17.978193508814602 28.454192620480335 35.735360991550245 17.832252879154815 22 16.918358714043993 29.121192893401016 35.76459390862944 18.19585448392555 23 17.473880123514235 28.926441351888666 35.699631995262465 17.90004652933463 24 18.023581663406123 29.64838999629884 35.300586897900914 17.02744144239412 25 17.484587972802927 28.80330763780943 35.66390677706225 18.048197612325392 26 17.274015481578616 28.333192335349604 37.429677255615246 16.963114927456537 27 15.824371054217822 29.748421687129216 36.0796506033015 18.34755665535146 28 17.31594864962037 26.71890531480659 37.45109235877588 18.514053676797158 29 16.073770145086925 28.839727591895436 37.99543166532718 17.091070597690454 30 17.411257150712164 28.32792293620666 35.52146006703958 18.739359846041598 31 18.022628740615417 26.85206725177117 36.63423918790314 18.491064819710267 32 16.777338380527418 28.85888299109692 37.68794806183517 16.675830566540487 33 17.654398003742983 28.670818486524208 35.77296805777303 17.90181545195978 34 17.383857393584403 27.931318855596203 36.79664206720095 17.88818168361845 35 17.53417705458813 29.030143474905106 36.697645404468126 16.738034066038633 36 17.090474629674663 28.211336554626293 34.095306569111536 20.60288224658751 37 18.275625099117196 27.602685415234973 36.64640270656024 17.475286779087593 38 16.52816544024359 28.32085765034255 37.65647466801996 17.494502241393896 39 16.884091677943168 28.870306156968876 34.75346752368065 19.492134641407308 40 16.856949553892342 27.058226563491054 38.8747938602055 17.210030022411097 41 17.240322205543457 28.514344912154595 35.57580498530624 18.669527896995707 42 17.66633544049557 27.951965157825747 35.34324192900484 19.03845747267384 43 17.42563582165282 27.2837783556373 36.168368533434816 19.122217289275067 44 16.814832824177333 29.212164776270868 35.17933214236636 18.79367025718544 45 17.04210314901534 27.473282523440556 35.34529233306202 20.13932199448209 46 19.21926365971481 27.26763421878799 33.956639852858785 19.556462268638416 47 18.142996067819542 28.07703691175849 34.69620734852649 19.083759671895482 48 16.983425647964147 28.871929305314787 32.98909137034375 21.15555367637732 49 19.264513128408947 27.3371104815864 34.5270813073443 18.871295082660353 50 17.099699801276902 27.533719504460702 35.42133525009513 19.945245444167263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1786.0 1 1064.0 2 342.0 3 371.5 4 401.0 5 390.5 6 380.0 7 280.0 8 180.0 9 219.5 10 259.0 11 386.0 12 513.0 13 860.5 14 1208.0 15 1440.5 16 1673.0 17 1917.5 18 2162.0 19 2001.0 20 1840.0 21 1727.5 22 1615.0 23 1691.5 24 1768.0 25 2097.5 26 2427.0 27 2952.5 28 3478.0 29 3846.0 30 4214.0 31 4708.0 32 5202.0 33 6185.0 34 7168.0 35 7037.5 36 6907.0 37 7229.0 38 7551.0 39 7605.5 40 7660.0 41 7327.0 42 6994.0 43 5989.5 44 4985.0 45 4792.5 46 4600.0 47 4659.0 48 4718.0 49 4125.0 50 3532.0 51 2998.0 52 2464.0 53 2804.5 54 3145.0 55 2469.0 56 1793.0 57 1524.0 58 1255.0 59 970.0 60 685.0 61 600.5 62 516.0 63 407.5 64 299.0 65 253.5 66 208.0 67 195.5 68 183.0 69 152.5 70 122.0 71 101.5 72 81.0 73 78.0 74 75.0 75 77.0 76 79.0 77 67.0 78 55.0 79 43.0 80 31.0 81 26.5 82 22.0 83 19.0 84 16.0 85 12.0 86 8.0 87 4.5 88 1.0 89 2.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011627415331275634 2 0.001057037757388694 3 0.0 4 0.002114075514777388 5 0.04228151029554776 6 0.032768170479049515 7 0.032768170479049515 8 0.023254830662551268 9 0.032768170479049515 10 0.03593928375121559 11 0.03699632150860429 12 0.03805335926599298 13 0.03593928375121559 14 0.04016743478077037 15 0.03699632150860429 16 0.03911039702338168 17 0.04439558581032514 18 0.046509661325102536 19 0.048623736839879916 20 0.04228151029554776 21 0.047566699082491226 22 0.046509661325102536 23 0.04228151029554776 24 0.041224472538159064 25 0.03699632150860429 26 0.04228151029554776 27 0.04333854805293645 28 0.04016743478077037 29 0.04228151029554776 30 0.0348822459938269 31 0.03593928375121559 32 0.03171113272166082 33 0.02854001944949474 34 0.023254830662551268 35 0.024311868419939958 36 0.02642594393471735 37 0.020083717390385183 38 0.02114075514777388 39 0.012684453088664328 40 0.008456302059109551 41 0.006342226544332164 42 0.006342226544332164 43 0.002114075514777388 44 0.003171113272166082 45 0.003171113272166082 46 0.001057037757388694 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 94604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.535960424506364 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40268442054499 41.3143207475371 2 6.698248084843053 6.63608304088622 3 2.616136397584448 3.887784871675616 4 1.506519002197896 2.9850746268656714 5 1.0776092012888632 2.6690203374064523 6 0.710581908968696 2.1119614392626103 7 0.5953524102170156 2.064394740180119 8 0.4651857542197469 1.843473848885882 9 0.3414207370420161 1.5221343706397192 >10 2.415551714572264 21.8119741237157 >50 0.12589889678424343 4.250348822459938 >100 0.02774043488466381 2.836032303073866 >500 0.017071036852100804 6.0673967274111025 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 0.980931038856708 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.9037672825673334 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 811 0.8572576212422307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.7589531098050822 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 683 0.721956788296478 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 634 0.670161938184432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.628937465646273 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 516 0.5454314828125661 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 477 0.5042070102744071 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 308 0.32556762927571775 RNA PCR Primer, Index 26 (100% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.245232759714177 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 224 0.23677645765506744 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 210 0.22197792905162572 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.22092089129423703 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 207 0.21880681577945965 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 177 0.18709568305779883 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 166 0.17546826772652319 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 122 0.12895860640142065 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.12684453088664327 No Hit AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG 119 0.12578749312925458 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 112 0.11838822882753372 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 99 0.10464673798148069 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 98 0.10358970022409199 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 97 0.10253266246670331 RNA PCR Primer, Index 26 (100% over 27bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.001057037757388694 0.0 13 0.0 0.0 0.0 0.001057037757388694 0.0 14 0.0 0.0 0.0 0.001057037757388694 0.0 15 0.0 0.0 0.0 0.001057037757388694 0.0 16 0.0 0.0 0.0 0.001057037757388694 0.0 17 0.0 0.0 0.0 0.001057037757388694 0.0 18 0.0 0.0 0.0 0.001057037757388694 0.0 19 0.0 0.0 0.0 0.001057037757388694 0.0 20 0.0 0.0 0.0 0.001057037757388694 0.0 21 0.0 0.0 0.0 0.002114075514777388 0.0 22 0.0 0.0 0.0 0.006342226544332164 0.0 23 0.0 0.0 0.0 0.012684453088664328 0.0 24 0.0 0.0 0.0 0.02431186841993996 0.0 25 0.0 0.0 0.0 0.02431186841993996 0.0 26 0.0 0.0 0.0 0.02959705720688343 0.0 27 0.0 0.0 0.0 0.032768170479049515 0.0 28 0.0 0.0 0.0 0.04333854805293645 0.0 29 0.0 0.0 0.0 0.05813707665637816 0.0 30 0.0 0.0 0.0 0.08879117162065028 0.0 31 0.0 0.0 0.0 0.1205023043423111 0.0 32 0.0 0.0 0.0 0.15115639930658323 0.0 33 0.0 0.0 0.0 0.18392456978563274 0.0 34 0.0 0.0 0.0 0.23466238214029006 0.0 35 0.0 0.0 0.0 0.2917424210392795 0.0 36 0.0 0.0 0.0 0.3498794976956577 0.0 37 0.0 0.0 0.0 0.40801657435203587 0.0 38 0.0 0.0 0.0 0.479895141854467 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCCGC 30 0.005715928 29.336153 7 TTATACC 30 0.005715928 29.336153 4 GTGGTAC 30 0.005715928 29.336153 1 CTTACAC 30 0.005715928 29.336153 3 GGCTGAA 30 0.00573073 29.320648 43 GGGGGTT 30 0.00573073 29.320648 37 TTATACA 160 0.0 28.877777 2 TATACAC 160 0.0 28.877777 3 TACTCCC 40 6.9800543E-4 27.502645 5 CATCTCG 185 0.0 24.975372 36 GCATCTC 185 0.0 24.975372 35 ATGGGAT 45 0.0013879377 24.446795 5 ATACACA 190 0.0 24.318127 4 CTCGTAT 190 0.0 24.305273 39 TCTCGTA 190 0.0 24.305273 38 ATCTCCG 195 0.0 23.694586 10 GCCCACG 195 0.0 23.694586 18 CTTATAC 195 0.0 23.694586 1 CCGAGCC 195 0.0 23.694586 14 CGAGCCC 195 0.0 23.694586 15 >>END_MODULE