FastQCFastQC Report
Mon 6 Feb 2017
SRR2077432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973159
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.10426620305203993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45950.024.5137981
GGTATCA21250.022.3625341
TATCAAC62100.017.890042
ATCAACG62050.017.8338473
TCAACGC62450.017.8259034
CAACGCA63950.017.4413035
AACGCAG65650.016.8891336
TATCGTA1201.181688E-414.66703112
ACGCAGA74850.014.548977
GAGTACT39350.014.08929512
AGAGTAC71350.014.06037811
CGCAGAG77050.014.0478968
GTACATG72850.013.7708711
TACATGG73900.013.3663692
ATACCGT2653.4924597E-1013.2826786
GTACTTT42000.013.2527114
CAGAGTA79300.013.15039110
AGTACTT42350.013.09123413
ACATGGG76600.012.7231253
GTCCTAA4700.012.6383991
Produced by FastQC (version 0.11.5)