FastQCFastQC Report
Mon 6 Feb 2017
SRR2077432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973159
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.12091516128131727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51750.024.1932471
GGTATCA23600.023.308821
ACCGGTC1203.1638592E-718.334428
TATCAAC71900.017.3818952
ATCAACG73750.016.9757083
TCAACGC73650.016.9392934
CAACGCA75000.016.6064435
AACGCAG77350.016.184546
ATACCGT2353.6379788E-1215.9158376
GTATTAC3300.014.6690171
GTACATG67500.014.6364181
CGTGCCG2153.5470293E-914.32667210
ACGCAGA89250.013.9277627
CGTTACG950.00623881913.896028524
TACATGG69550.013.888172
CGCAGAG92000.013.5355858
TATACCG1000.00875127413.2030045
GTGCCGA2502.075467E-913.20122711
AGAGTAC89900.013.06906811
ACATGGG70800.013.0214213
Produced by FastQC (version 0.11.5)