FastQCFastQC Report
Mon 6 Feb 2017
SRR2077431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947359
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.024.7024881
GTATCAA38700.024.0472281
TCAACGC49600.018.6723584
CAACGCA50600.018.3465025
ATCAACG51100.018.079663
TATCAAC51800.017.8799292
AACGCAG52750.017.4730286
TAACGGA1206.41515E-616.49936918
ACGCAGA60850.015.1481497
GTATAGA6450.015.0081991
GTATAAT7350.014.9664311
CGCAGAG61900.014.7848238
GTACATG73200.014.5168261
GTATTAC3650.014.4661841
TACATGG73350.014.1864742
AGAGTAC60750.014.05216211
TATACCG1100.001019164813.998995
GTATAAG6950.013.6119161
ACATGGG74650.013.4957663
GAGTACT30300.013.21558712
Produced by FastQC (version 0.11.5)