FastQCFastQC Report
Mon 6 Feb 2017
SRR2077431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947359
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.025.6335091
GTATCAA44900.024.7962681
CGTTACG1451.2732926E-1121.24248524
GCGATAC1452.9649527E-1019.72717314
TGCGATA1303.5861376E-818.6182513
TATCAAC64600.017.2339732
ATCAACG65600.017.038633
TCAACGC65650.016.958914
TATACCG1307.3137926E-716.9256825
CAACGCA66400.016.6682285
AACGCAG68800.016.0534346
TACCGTA1401.5874357E-615.7148387
ATAGCGT900.004358982714.667686
CGCGCAA900.004359474414.66743121
CGAATCG1351.9606669E-514.66444437
GTACATG71300.014.1337731
ATACCGA1453.836979E-513.6561166
TACATGG72300.013.6030612
TATACTG7500.013.495415
ACGCAGA82100.013.345177
Produced by FastQC (version 0.11.5)