FastQCFastQC Report
Mon 6 Feb 2017
SRR2077430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965308
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39650.024.0797651
GGTATCA17100.022.5137391
CCGTATA600.00741191918.3344739
TCAACGC52900.017.8405114
TATCAAC52900.017.839612
ATCAACG53200.017.780363
CAACGCA54100.017.4041255
AACGCAG55800.016.911896
GTACATG68300.015.6538541
TACATGG69600.014.9814152
GTATAGG4400.014.49943351
ACGCAGA65600.014.2865097
CGCAGAG66900.013.8447048
ACATGGG74400.013.4826613
AGAGTAC63550.013.468080511
GTCTTAG6650.013.2325651
CAGAGTA68850.012.65502210
ATACCGT2105.937691E-712.5698776
GAGTACT35200.012.37639612
GCAGAGT71150.012.3690059
Produced by FastQC (version 0.11.5)