##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077430_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965308
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.578429289638716	31.0	30.0	34.0	16.0	34.0
2	28.529620194596987	31.0	29.0	34.0	16.0	34.0
3	28.64877240408079	31.0	30.0	34.0	16.0	34.0
4	31.969401492189007	35.0	33.0	37.0	18.0	37.0
5	32.04714889650586	35.0	33.0	37.0	18.0	37.0
6	32.04606536656563	36.0	33.0	37.0	18.0	37.0
7	31.87576838561121	36.0	33.0	37.0	17.0	37.0
8	31.811101241422477	36.0	33.0	37.0	17.0	37.0
9	32.992258814261454	38.0	33.0	39.0	13.0	39.0
10	32.877077861726335	38.0	33.0	39.0	13.0	39.0
11	32.93781354247181	38.0	33.0	39.0	13.0	39.0
12	32.84818305552071	38.0	33.0	39.0	11.0	39.0
13	32.851258621364124	38.0	33.0	39.0	11.0	39.0
14	33.66571364593492	39.0	33.0	41.0	10.0	41.0
15	33.74981654519531	39.0	33.0	41.0	10.0	41.0
16	33.643838683873646	39.0	33.0	41.0	10.0	41.0
17	33.45510483228049	39.0	32.0	41.0	10.0	41.0
18	33.26322358419429	38.0	32.0	41.0	10.0	41.0
19	33.60301358240021	39.0	33.0	41.0	10.0	41.0
20	33.64312981990404	39.0	33.0	41.0	10.0	41.0
21	33.63625532322443	39.0	33.0	41.0	10.0	41.0
22	33.53485438949343	39.0	33.0	41.0	10.0	41.0
23	33.27087742656075	39.0	32.0	41.0	8.0	41.0
24	33.421952120993836	39.0	33.0	41.0	8.0	41.0
25	33.321510278190324	39.0	32.0	41.0	8.0	41.0
26	33.354083623016564	39.0	33.0	41.0	8.0	41.0
27	33.33401285802352	39.0	33.0	41.0	2.0	41.0
28	33.20736429402949	39.0	32.0	41.0	2.0	41.0
29	33.165641478052194	39.0	32.0	41.0	2.0	41.0
30	32.938748015383226	38.0	32.0	41.0	2.0	41.0
31	32.863169019879216	38.0	32.0	40.0	2.0	41.0
32	32.87834484647126	38.0	32.0	40.0	2.0	41.0
33	32.85370187515091	38.0	32.0	40.0	2.0	41.0
34	32.83783202284552	38.0	32.0	40.0	2.0	41.0
35	32.76339624753989	38.0	32.0	40.0	2.0	41.0
36	32.63067748780228	38.0	32.0	40.0	2.0	41.0
37	32.53359280047806	38.0	31.0	40.0	2.0	41.0
38	32.42437109399766	38.0	31.0	40.0	2.0	41.0
39	32.35327729868196	38.0	31.0	40.0	2.0	41.0
40	32.30943767055564	38.0	31.0	40.0	2.0	41.0
41	32.183307433831494	38.0	31.0	40.0	2.0	41.0
42	31.995236245273677	38.0	31.0	40.0	2.0	41.0
43	31.888223752810838	38.0	31.0	40.0	2.0	41.0
44	31.815914569414037	38.0	31.0	40.0	2.0	41.0
45	31.71679670374882	38.0	31.0	40.0	2.0	41.0
46	31.55481353033142	38.0	30.0	40.0	2.0	41.0
47	31.366115762679627	38.0	30.0	40.0	2.0	41.0
48	31.175079957967267	38.0	30.0	40.0	2.0	41.0
49	31.040272713660773	38.0	30.0	40.0	2.0	41.0
50	30.83193078088347	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.950874799365149
1101	2	0.9471993570186754
1101	3	1.0311952318267963
1101	4	1.1026949214902046
1101	5	0.8890596862678706
1101	6	1.0422847889508873
1101	7	1.0605310320517596
1101	8	1.0484316806582328
1101	9	1.3575258194222357
1101	10	1.4091027727474312
1101	11	1.3445758209849927
1101	12	1.2943270840640722
1101	13	1.2073141273653647
1101	14	1.4584004230076957
1101	15	1.4358052649899875
1101	16	1.3884603368178858
1101	17	1.5652916526001803
1101	18	1.7993473814703478
1101	19	1.3888700669169864
1101	20	1.4168478160275697
1101	21	1.4574588871225984
1101	22	1.312860651321742
1101	23	1.422052450815059
1101	24	1.4854341724739584
1101	25	1.3579625700371878
1101	26	1.3640248040205307
1101	27	1.4746188038217554
1101	28	1.6261058520480631
1101	29	1.4693446535928274
1101	30	1.6420097966382414
1101	31	1.72183410997134
1101	32	1.6398176022257047
1101	33	1.6247255929222248
1101	34	1.5435414770998293
1101	35	1.5721948345489523
1101	36	1.5489249863697623
1101	37	1.656736306488419
1101	38	1.624959547042927
1101	39	1.6943083932505445
1101	40	1.5436255551660452
1101	41	1.6557215754140984
1101	42	1.7258777682516708
1101	43	1.8246290967173024
1101	44	1.5624234194773479
1101	45	1.6869686285450207
1101	46	1.7065776944689297
1101	47	1.7038757733583303
1101	48	1.751612371811781
1101	49	1.8279055859750564
1101	50	1.8687188797637901
1102	1	0.38175450347980444
1102	2	0.3439057832784975
1102	3	0.4015353150445513
1102	4	0.35290148644628516
1102	5	0.2517881190001887
1102	6	0.4921980575592997
1102	7	0.5332333898880997
1102	8	0.48255502786118853
1102	9	0.5092750565928341
1102	10	0.5624412840742963
1102	11	0.5445299587566055
1102	12	0.5225231081047568
1102	13	0.6266679877444687
1102	14	0.8915491978574437
1102	15	0.8226631475604904
1102	16	0.9268784597952262
1102	17	0.9054477321193701
1102	18	0.9466657356081214
1102	19	0.838512970291923
1102	20	0.7866334840812002
1102	21	0.6660979994665652
1102	22	0.9208957877063924
1102	23	1.0728555945968452
1102	24	0.9682791100606423
1102	25	0.9339392690663146
1102	26	0.7962918405438728
1102	27	0.776600495916071
1102	28	0.7892599898196764
1102	29	0.6520131695428972
1102	30	0.8472225566937226
1102	31	0.9773041053481322
1102	32	0.9782930529885334
1102	33	0.73726669324585
1102	34	0.6365959384960433
1102	35	0.8307723657556139
1102	36	0.7517018703272313
1102	37	0.7699061631689545
1102	38	0.8135061952167604
1102	39	0.9125641491451333
1102	40	0.8004662394009046
1102	41	0.8893929577534436
1102	42	0.8447500752327173
1102	43	0.8822020971796221
1102	44	0.8250830588670865
1102	45	0.7973978472227863
1102	46	0.7942877268313886
1102	47	0.8014672666546723
1102	48	0.7693754786078983
1102	49	0.7903237089524033
1102	50	0.5643382972395159
1103	1	0.582715013033944
1103	2	0.5609685676279064
1103	3	0.7205354970281697
1103	4	0.6494106766191692
1103	5	0.5830467177263046
1103	6	0.6946865015993353
1103	7	0.6965549549472421
1103	8	0.8272207239485425
1103	9	0.9118293787038425
1103	10	0.9526345506803011
1103	11	0.8403184937884589
1103	12	0.9023010880865598
1103	13	0.9704667138591176
1103	14	1.1992121641904774
1103	15	1.1151901721282798
1103	16	1.2665570767197067
1103	17	1.3377090605998063
1103	18	1.0393765636336028
1103	19	0.9817752086904719
1103	20	1.0198025468655487
1103	21	1.0124426764456373
1103	22	1.1216328213733604
1103	23	1.3826867137961187
1103	24	1.1309062256202438
1103	25	0.8481478222965251
1103	26	1.0473629961398743
1103	27	0.9287231528769411
1103	28	1.153695658609486
1103	29	1.1720532059396191
1103	30	1.2035286531450424
1103	31	1.070846598523751
1103	32	1.1333929619967194
1103	33	1.1582519525725132
1103	34	1.0608667301247934
1103	35	0.9980939308147541
1103	36	1.0813404508549809
1103	37	0.9856775917403837
1103	38	1.0536907266089344
1103	39	1.1962806186628754
1103	40	1.1427344832589554
1103	41	1.2534442771346974
1103	42	1.2144603573073312
1103	43	1.231584080801099
1103	44	1.1063711389398776
1103	45	1.072035517832429
1103	46	1.099250056221745
1103	47	1.0495025714772446
1103	48	0.9690872165514861
1103	49	0.9471306243300184
1103	50	1.104301536547979
1104	1	0.6180850054103075
1104	2	0.5614360278087425
1104	3	0.5093764990857323
1104	4	0.5726412547860562
1104	5	0.5616962271082997
1104	6	0.6737944539557006
1104	7	0.657788344843059
1104	8	0.6660682839744467
1104	9	0.8031497026674828
1104	10	0.8946358786688862
1104	11	0.8931423139689656
1104	12	0.7945282561099063
1104	13	0.7525694034458894
1104	14	0.9380058259140753
1104	15	0.9791053612027056
1104	16	1.0941003388171069
1104	17	1.177255454127092
1104	18	1.282736005878391
1104	19	1.1462537682327252
1104	20	1.1373276925754041
1104	21	0.9604408566094236
1104	22	1.0018224286330337
1104	23	1.210984552125801
1104	24	1.1018062657878005
1104	25	1.1582630785901245
1104	26	1.1108679023199386
1104	27	1.0668892990048704
1104	28	1.200766296125984
1104	29	1.1643066058363303
1104	30	1.3441327240038916
1104	31	1.2910088672528985
1104	32	1.304561264056744
1104	33	1.1887717125508743
1104	34	1.0121014726015787
1104	35	1.1099064712897189
1104	36	1.13648230790767
1104	37	1.1466814527442466
1104	38	1.1544899790005445
1104	39	1.04679992778091
1104	40	1.1255812973159607
1104	41	1.216838517599001
1104	42	1.2929377200203618
1104	43	1.3130623288398553
1104	44	1.2341484385324648
1104	45	1.1802374868624206
1104	46	1.2769554230990856
1104	47	1.2734600594474692
1104	48	1.2407250281574491
1104	49	1.195772100200795
1104	50	1.3148219523231717
1105	1	0.5733292128284262
1105	2	0.634555886798168
1105	3	0.5168586467852734
1105	4	0.5625950045224144
1105	5	0.6006059562354196
1105	6	0.4640758724979861
1105	7	0.5596895771790678
1105	8	0.6150235101035832
1105	9	0.8540769677251845
1105	10	0.8480903364711736
1105	11	0.8278925324148361
1105	12	0.8312181232345566
1105	13	0.732834702189443
1105	14	1.0294427477857795
1105	15	0.76907040986341
1105	16	0.928906298623069
1105	17	0.8267439894851947
1105	18	1.067844776856731
1105	19	0.8580159484731666
1105	20	0.9219016478015334
1105	21	0.8190250260631586
1105	22	0.9078937173130157
1105	23	1.2483711862515676
1105	24	0.8925554597978618
1105	25	0.9272151410204472
1105	26	0.8920604661211939
1105	27	0.8958597410187963
1105	28	0.9395547501318262
1105	29	0.8237449739703493
1105	30	1.0655946541614725
1105	31	1.119910762459142
1105	32	0.9681546233022758
1105	33	1.0126463396863699
1105	34	0.9324433027260142
1105	35	0.919629572317163
1105	36	1.0048423067024501
1105	37	0.8369914952282045
1105	38	0.908220130556785
1105	39	0.9887008588122796
1105	40	1.0292843314537805
1105	41	1.1391636855763423
1105	42	1.2478196085979008
1105	43	1.0401375646107383
1105	44	0.9154441991760507
1105	45	0.825436197278524
1105	46	0.8495999740045086
1105	47	0.9982815364428568
1105	48	1.0252613203024339
1105	49	0.9252555133798559
1105	50	0.8098617245368587
1106	1	0.4217407256038932
1106	2	0.41394155276403666
1106	3	0.45026907525651794
1106	4	0.3718163903985925
1106	5	0.44349207130753854
1106	6	0.47608430176332206
1106	7	0.4274083512482214
1106	8	0.487361740159713
1106	9	-0.8922239056504822
1106	10	-0.39808192193144976
1106	11	0.14277098326532922
1106	12	0.4400978486655873
1106	13	0.5288143335161664
1106	14	0.6884654266055108
1106	15	0.6469712067347047
1106	16	0.7655476582937908
1106	17	0.7437385689486931
1106	18	-1.0773830168758707
1106	19	0.021789455167173344
1106	20	0.2124998563488063
1106	21	0.5369809316811853
1106	22	0.5977692697112502
1106	23	-1.0272851870326534
1106	24	0.1591228283207471
1106	25	0.3099558731731804
1106	26	0.5166564243372065
1106	27	0.5787706880114527
1106	28	0.6866105749378022
1106	29	0.6197111161094639
1106	30	-0.5315898963864285
1106	31	0.43838319565504236
1106	32	0.5338993236884662
1106	33	0.6341774461714422
1106	34	0.7076989634463615
1106	35	0.6754034984559851
1106	36	0.746329558558628
1106	37	0.6354899123409581
1106	38	0.5423445259741015
1106	39	0.6865236103948575
1106	40	0.6525635677294517
1106	41	0.7082735936554059
1106	42	0.7931876111126002
1106	43	0.7156039077338292
1106	44	0.6610242379353082
1106	45	0.6091118679779193
1106	46	0.71270362438052
1106	47	0.7458251381494883
1106	48	0.8012825662652148
1106	49	0.9221490125514329
1106	50	0.7022587211741609
1107	1	0.34144250925561437
1107	2	0.3448957070001306
1107	3	0.3744995489648808
1107	4	0.35724353999809466
1107	5	0.3432222653416517
1107	6	0.322476062393406
1107	7	0.45923857556158154
1107	8	0.15726252699795396
1107	9	0.5155810364402811
1107	10	0.5770792976976722
1107	11	0.45602409819220924
1107	12	0.5073166542641587
1107	13	0.38708379588710784
1107	14	0.7256788648066248
1107	15	0.6799733023145507
1107	16	0.5054220263583105
1107	17	0.772602630571825
1107	18	0.780412340749848
1107	19	0.6269887715287012
1107	20	0.5964081144607078
1107	21	0.5611549853723332
1107	22	0.6533715387187229
1107	23	0.8456747301086622
1107	24	0.6821457516150886
1107	25	0.7664041464872895
1107	26	0.7257657269738615
1107	27	0.6600556360736505
1107	28	0.636791345565662
1107	29	0.5008260521118757
1107	30	0.7713794829579683
1107	31	0.8759077905664512
1107	32	0.7566377307673591
1107	33	0.6808229100898728
1107	34	0.616073127182986
1107	35	0.5453931775081315
1107	36	0.6377347798918507
1107	37	0.7060530194328862
1107	38	0.6393614358232185
1107	39	0.5792653200023992
1107	40	0.7054538527982714
1107	41	0.5540720926374547
1107	42	0.5777377639667449
1107	43	0.5434952007361389
1107	44	0.6015786736456263
1107	45	0.6242497918503354
1107	46	0.7790648495987575
1107	47	0.8124844496913717
1107	48	0.665538256019456
1107	49	0.6012379318861392
1107	50	0.6985672830197238
1108	1	-0.004279019831418651
1108	2	0.0585973650779934
1108	3	-0.021161772999299444
1108	4	-0.055028452200197364
1108	5	0.04304201043250089
1108	6	-0.023768282096249038
1108	7	0.023914706652870876
1108	8	0.035389485489890404
1108	9	0.030139462211195678
1108	10	-0.004602273806675328
1108	11	0.08101211640710915
1108	12	-0.11248215521705163
1108	13	0.044016295072630385
1108	14	-0.21251179438772283
1108	15	0.050098296584131674
1108	16	0.16909680356177148
1108	17	-0.045201518869447455
1108	18	0.3410519575396691
1108	19	0.042095866053237785
1108	20	0.01841039398184563
1108	21	0.14083108987735926
1108	22	0.08993748940996227
1108	23	-0.19062472556261412
1108	24	0.026753531562405897
1108	25	0.12579087453518412
1108	26	0.11320470609558697
1108	27	0.028835221382450982
1108	28	0.11114845722756428
1108	29	0.14498348939090988
1108	30	0.24542014615462193
1108	31	0.21260795162176294
1108	32	0.15016252260764418
1108	33	0.1327846103407495
1108	34	0.04097905849354788
1108	35	0.10702582740187694
1108	36	0.2882319646932956
1108	37	0.0817877166274883
1108	38	0.0881794893087573
1108	39	0.2924486176742036
1108	40	0.33301240402764876
1108	41	0.30741028622832545
1108	42	0.29312111139080343
1108	43	0.37668690829177365
1108	44	0.3092877865135364
1108	45	0.20702470573597154
1108	46	0.1963686885555198
1108	47	0.22422514762262935
1108	48	0.15858882066152802
1108	49	0.14512058210323175
1108	50	0.2606313490318506
1109	1	-0.04291581609784245
1109	2	-0.06505588614763624
1109	3	0.031582386363783854
1109	4	0.00867576403836523
1109	5	-0.026146191080719916
1109	6	-0.023400105572122243
1109	7	0.02542249988153955
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2211	4	0.21990186966962
2211	5	0.03397453586201493
2211	6	0.0349134929435877
2211	7	0.010646887865533472
2211	8	0.08378406767383595
2211	9	0.2792647521431846
2211	10	-0.05708028288334077
2211	11	0.09822908585901757
2211	12	0.12157101102150847
2211	13	0.030230772500701164
2211	14	0.290128035413332
2211	15	0.021488269979059282
2211	16	0.0118583192800088
2211	17	0.29471441297949497
2211	18	0.5478021205346693
2211	19	0.3267619802983148
2211	20	0.27329271337649175
2211	21	0.25253155279848016
2211	22	0.18342080367403213
2211	23	0.4332139509945492
2211	24	0.38995285926226586
2211	25	0.3773852132860327
2211	26	0.19233220673505969
2211	27	0.24623609310576455
2211	28	0.186444235082611
2211	29	0.254838802925903
2211	30	0.3958559822845231
2211	31	0.3977702752438148
2211	32	0.3365843878831427
2211	33	0.333033863780237
2211	34	0.160926532492212
2211	35	0.18029316626657277
2211	36	0.30554440811731354
2211	37	0.22093413847234444
2211	38	0.3252622948548307
2211	39	0.20509197541722557
2211	40	0.03126002266682093
2211	41	0.23315859539449235
2211	42	0.3294093896887489
2211	43	0.1631351182569034
2211	44	0.21688991430672644
2211	45	0.30319099178314346
2211	46	-0.10747493018372367
2211	47	0.17189571034213458
2211	48	0.2786302369642968
2211	49	0.3642267108685182
2211	50	0.38189605751203004
2212	1	0.03540510933327923
2212	2	-0.023113169061026895
2212	3	-0.15757495650208497
2212	4	-0.009892573082701972
2212	5	0.07082387367613308
2212	6	0.07354855777678893
2212	7	-0.022873439168009213
2212	8	0.06025484765861577
2212	9	-0.06200622098126729
2212	10	0.11004563379156451
2212	11	0.030226298780220873
2212	12	0.019865555131410417
2212	13	-0.06498219556504381
2212	14	-0.054488756532663274
2212	15	-0.013481113919652898
2212	16	-0.0997929128512638
2212	17	0.25729054576505206
2212	18	0.33557360859637697
2212	19	0.06980523400906691
2212	20	-0.021559543943411086
2212	21	0.1277527313375657
2212	22	0.17880419508848178
2212	23	0.3843463545545944
2212	24	0.28851836936593145
2212	25	0.04329280165812577
2212	26	0.16371931832924957
2212	27	-0.0015011880458999372
2212	28	-0.06632730900589934
2212	29	-0.05593015985498084
2212	30	0.26702947895805806
2212	31	0.07767335906093109
2212	32	-0.01989786231625601
2212	33	-0.02387979280398156
2212	34	-0.12151782525216959
2212	35	-0.11809081699465196
2212	36	-0.06682857147976762
2212	37	-1.2369036547643049
2212	38	-0.6374060274887619
2212	39	-0.19531376050502303
2212	40	-0.16081819482515414
2212	41	-0.9450855857126612
2212	42	-0.33579480763561165
2212	43	-0.1938666532087474
2212	44	-0.06933319664773663
2212	45	0.036378621472458406
2212	46	-0.028036563232610945
2212	47	0.12329449588477459
2212	48	0.11241297317075194
2212	49	0.11909060139010919
2212	50	0.2661681114444505
2213	1	-0.5230554860114474
2213	2	-0.5738851544726273
2213	3	-0.6120310075121651
2213	4	-0.7701514405488297
2213	5	-0.6534110155735888
2213	6	-0.6667170441282799
2213	7	-0.5711172808172229
2213	8	-0.6415393777764429
2213	9	-0.6481777714964281
2213	10	-0.7805298972324977
2213	11	-0.8366929626750519
2213	12	-0.6336709946336967
2213	13	-0.8253855203225413
2213	14	-2.3497281217939125
2213	15	-1.5212371058857599
2213	16	-1.2051493327497411
2213	17	-0.8159413016940675
2213	18	-0.983023230207543
2213	19	-0.9451731519804341
2213	20	-1.0685130573047203
2213	21	-1.065370480474087
2213	22	-1.0964572443761966
2213	23	-1.550010746504718
2213	24	-1.2909008615039212
2213	25	-1.142263389028507
2213	26	-1.2364802680053018
2213	27	-1.0995239771011782
2213	28	-1.061684536359472
2213	29	-1.2907789427152494
2213	30	-1.122689095652202
2213	31	-1.0700721943366034
2213	32	-1.2100675577551172
2213	33	-1.269009229360318
2213	34	-1.1449424926098501
2213	35	-1.2582041317408326
2213	36	-1.0691082675228039
2213	37	-1.2036653024921655
2213	38	-1.0933137095863543
2213	39	-1.0927958558001585
2213	40	-1.3451111606114594
2213	41	-1.6149208705212672
2213	42	-1.2763183058020857
2213	43	-1.3865833142069164
2213	44	-1.2930582034188767
2213	45	-1.2520460298866922
2213	46	-1.3062381555942757
2213	47	-1.215957571077908
2213	48	-1.3352957459702068
2213	49	-1.4778880484809918
2213	50	-1.4076737692746697
2214	1	-0.8808189916747295
2214	2	-0.7651606469743193
2214	3	-0.9527920732957647
2214	4	-1.058627650541272
2214	5	-0.9492205692086699
2214	6	-0.9001217370052181
2214	7	-0.9973164068163705
2214	8	-0.9364903574831906
2214	9	-1.0638819609242063
2214	10	-1.230581442225727
2214	11	-1.1697929784816274
2214	12	-1.17415189418805
2214	13	-1.2464931140255757
2214	14	-1.6080386349816607
2214	15	-1.3802477753706341
2214	16	-1.1972927608950599
2214	17	-1.6534183129419269
2214	18	-1.7760480670882188
2214	19	-1.2920274350762995
2214	20	-1.3089680199999947
2214	21	-1.2765482296306772
2214	22	-1.1479789027647058
2214	23	-1.0053113339592983
2214	24	-0.9264370160131534
2214	25	-1.1227947714705095
2214	26	-1.241990669926608
2214	27	-1.1815389923294717
2214	28	-1.1707978745073078
2214	29	-1.156261233563093
2214	30	-0.9789843584112887
2214	31	-0.920534395461992
2214	32	-1.014684624245426
2214	33	-1.0837103725729875
2214	34	-1.0791660253693252
2214	35	-1.0712016001885658
2214	36	-1.152304145997551
2214	37	-1.1524105131784488
2214	38	-1.0947442648731176
2214	39	-1.0588130145001244
2214	40	-1.1156665844198272
2214	41	-0.963234182963749
2214	42	-0.8496908651237405
2214	43	-0.9012123138747654
2214	44	-0.9337785350921344
2214	45	-0.8713216905473864
2214	46	-0.8517631306783144
2214	47	-0.9125918816440617
2214	48	-1.0390888055778476
2214	49	-1.020297452124911
2214	50	-0.906082833267039
2215	1	-0.26873141426471037
2215	2	-0.41524434025005874
2215	3	-0.34822594058690015
2215	4	-0.13255725501570836
2215	5	-0.31393477011476634
2215	6	-0.24839034528912052
2215	7	-0.2731584717599169
2215	8	-0.2865462074243794
2215	9	-0.2936552151699914
2215	10	-0.7249841482756523
2215	11	-0.3918876549623249
2215	12	-0.5251815859804054
2215	13	-0.49462089468541137
2215	14	-0.4872459772624538
2215	15	-0.5959741435309169
2215	16	-0.37778958264046025
2215	17	-0.48148021440002964
2215	18	-0.4523104597123293
2215	19	-0.7762946448802879
2215	20	-0.7020450219498997
2215	21	-0.6553780650589403
2215	22	-1.005766034703143
2215	23	-0.9430674398458194
2215	24	-0.6945436191938228
2215	25	-0.5258501801416458
2215	26	-0.613137486588812
2215	27	-0.6616609195758656
2215	28	-0.6864657477184721
2215	29	-0.8316874553662714
2215	30	-0.6015236139210103
2215	31	-0.7266835708617165
2215	32	-0.8587813257354924
2215	33	-0.8674647120731436
2215	34	-0.7368383879100051
2215	35	-0.7598624838302044
2215	36	-0.773035348100322
2215	37	-0.6954152726688569
2215	38	-0.5869235097679812
2215	39	-0.7984032102503029
2215	40	-0.7326244160270861
2215	41	-0.8653657110446993
2215	42	-0.8501096253921929
2215	43	-0.8757116944204384
2215	44	-0.8780231175607618
2215	45	-0.7813757031332678
2215	46	-0.7389024490151428
2215	47	-0.8618556224602791
2215	48	-0.9204979700187543
2215	49	-0.9659856646851495
2215	50	-0.9743592176565734
2216	1	-1.0799565762741032
2216	2	-1.346486721095829
2216	3	-1.1991764533135232
2216	4	-2.5382184096986578
2216	5	-1.6555683408970836
2216	6	-1.4157469444162025
2216	7	-1.3586442869194855
2216	8	-1.422043899722489
2216	9	-1.4198044941211698
2216	10	-1.7124834097226227
2216	11	-1.50613929676069
2216	12	-1.6609894504548208
2216	13	-1.6536086730897566
2216	14	-1.547283968028431
2216	15	-1.7008375805537455
2216	16	-1.8216108781287979
2216	17	-1.5772083834758206
2216	18	-1.4636603015313483
2216	19	-1.849221883238581
2216	20	-1.862670438704825
2216	21	-1.915556772786072
2216	22	-1.9067341722191067
2216	23	-3.6020038511332437
2216	24	-2.55313774380852
2216	25	-2.058719668677096
2216	26	-2.2786555207475416
2216	27	-2.2919369314136127
2216	28	-2.487069050085502
2216	29	-2.2262398078234753
2216	30	-1.9410698630161605
2216	31	-1.8811727473921707
2216	32	-1.9988871468195626
2216	33	-2.231657932819509
2216	34	-2.478530279260916
2216	35	-2.498178856234567
2216	36	-2.345975930654877
2216	37	-2.185909019988461
2216	38	-2.413904630543808
2216	39	-2.553433751176591
2216	40	-2.5223038458279703
2216	41	-2.5701194201886466
2216	42	-2.9030789703167557
2216	43	-2.6663881607647255
2216	44	-2.6843934439268438
2216	45	-2.6288213546737005
2216	46	-2.678515298511499
2216	47	-2.7393643414053805
2216	48	-2.7032042132135174
2216	49	-3.1427063525704284
2216	50	-3.0182277805388864
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	193264.0
3	9715.0
4	17965.0
5	13557.0
6	11069.0
7	9343.0
8	8330.0
9	7353.0
10	7165.0
11	6818.0
12	7104.0
13	7299.0
14	7615.0
15	8216.0
16	8771.0
17	9330.0
18	10285.0
19	11929.0
20	14276.0
21	18722.0
22	22852.0
23	26897.0
24	29133.0
25	31052.0
26	33711.0
27	38131.0
28	43377.0
29	49227.0
30	56557.0
31	66587.0
32	80087.0
33	97922.0
34	123141.0
35	156641.0
36	204984.0
37	278176.0
38	436908.0
39	801799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89675681457664	17.556757347723345	12.620122509015411	24.92636332868461
2	15.885908696856731	19.753248063613825	38.82248013333419	25.538363106195256
3	18.736915773688608	24.673054626096512	29.11571888839248	27.474310711822405
4	12.732814864512784	16.145576703191676	37.247847175442885	33.87376125685266
5	14.067851525849326	37.36828960424443	34.37953519139773	14.184323678508509
6	32.522747327094166	36.45038779700594	17.369897774110985	13.656967101788911
7	28.449926433103723	31.799860254948687	21.752083102083727	17.99813020986387
8	26.676557683275114	34.54976866223607	19.833501595339772	18.940172059149045
9	26.927051964024518	15.461292276338408	18.931701580970987	38.67995417866609
10	14.795093847196833	28.636344769707307	33.613227593751866	22.955333789343996
11	35.19066807973512	22.81370001582448	23.43922236697064	18.556409537469765
12	23.61442994002317	24.622356546444163	30.017942767397688	21.74527074613498
13	28.1950914001644	21.723078355917355	25.748383979224226	24.33344626469401
14	22.591347888163778	20.87951247380937	26.636203591250364	29.89293604677649
15	23.84945216829309	28.682790845228475	23.450976951075667	24.01678003540277
16	23.980863743604402	27.776425997689635	24.854691139998334	23.38801911870763
17	22.919918766827248	27.555353346413174	26.300269100916722	23.224458785842856
18	23.05292628009568	27.086381822992315	26.97467886242515	22.886013034486854
19	23.835776476912603	26.728741040053965	26.943533400127627	22.4919490829058
20	23.95799442445679	26.297056302186345	26.88377084922408	22.861178424132785
21	24.174653312034863	26.10717567897314	26.82294470373784	22.89522630525416
22	23.93273992828517	25.939405188778743	26.71308162834845	23.41477325458764
23	23.872447827711223	25.990994228895616	26.58106358271483	23.55549436067833
24	23.49574967201343	26.174137921725936	26.75712392223344	23.57298848402719
25	23.748835670990996	26.01337122183674	26.716423451273673	23.521369655898592
26	23.478543506010897	26.29940198178336	26.900424127831034	23.321630384374707
27	23.531647154552214	26.315620727559043	26.654125454644245	23.498606663244495
28	23.577389184461502	26.250201271054134	26.742771035736624	23.42963850874774
29	23.6697836685483	26.34432519789542	26.654011441008063	23.331879692548224
30	23.847859469128093	26.241001670575926	26.649364674912672	23.261774185383306
31	23.943855428842955	25.990200922266137	26.49422773710015	23.571715911790754
32	23.513678340880382	26.316778879563035	26.568114617466463	23.60142816209012
33	23.64670008960407	26.14255315917632	26.572956444996432	23.63779030622318
34	23.85622602251357	26.161810345147572	26.565595519017148	23.416368113321706
35	23.828861317618134	26.257017179411978	26.520407154190124	23.393714348779763
36	23.885700746005185	26.251639544012683	26.399510718888152	23.463148991093977
37	23.907506123231553	25.914636616249588	26.660259889660313	23.51759737085855
38	23.850966222785907	26.141963319342036	26.53608650325528	23.470983954616774
39	23.86833872299993	26.311234350621653	26.397747947157875	23.42267897922054
40	23.953861592877114	26.117168851344925	26.577095195642475	23.35187436013549
41	23.70235676238898	26.30634874252693	26.50188518046377	23.48940931462032
42	23.93120545670073	26.29151736650887	26.430539869595542	23.346737307194854
43	23.796495122556006	25.948980400822496	26.480936388279293	23.77358808834221
44	23.733421137682573	26.308614294880922	26.310436055200686	23.64752851223582
45	23.812340064471833	26.24462523848	26.250293092134676	23.69274160491349
46	23.963117938323656	26.034200012077658	26.245930116245763	23.756751933352923
47	23.851395570068615	26.23108373420412	26.200116044501716	23.71740465122555
48	23.77880044400509	26.445766263035903	26.179835556170357	23.59559773678866
49	23.980398163981153	26.16714870484013	26.10810296497339	23.744350166205322
50	23.869933412076918	26.30707096470337	26.129611130172865	23.693384493046846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1638.0
1	1402.5
2	1167.0
3	1187.5
4	1208.0
5	1219.5
6	1231.0
7	1191.0
8	1151.0
9	1290.5
10	1430.0
11	1831.5
12	2233.0
13	3627.0
14	5021.0
15	7044.0
16	9067.0
17	10137.0
18	11207.0
19	11915.5
20	12624.0
21	14912.5
22	17201.0
23	21116.0
24	25031.0
25	30596.5
26	36162.0
27	42327.5
28	48493.0
29	55253.0
30	62013.0
31	69230.0
32	76447.0
33	85403.0
34	94359.0
35	104259.0
36	114159.0
37	122998.5
38	131838.0
39	140327.5
40	148817.0
41	157742.0
42	166667.0
43	173743.5
44	180820.0
45	188734.5
46	196649.0
47	202424.0
48	208199.0
49	210110.0
50	212021.0
51	211052.0
52	210083.0
53	204782.0
54	199481.0
55	191982.0
56	184483.0
57	172243.0
58	160003.0
59	146579.0
60	133155.0
61	117443.5
62	101732.0
63	87776.0
64	73820.0
65	62094.0
66	50368.0
67	41438.5
68	32509.0
69	26693.0
70	20877.0
71	16630.5
72	12384.0
73	10222.0
74	8060.0
75	6402.5
76	4745.0
77	3744.0
78	2743.0
79	2222.5
80	1702.0
81	1356.5
82	1011.0
83	787.5
84	564.0
85	423.5
86	283.0
87	225.0
88	167.0
89	138.5
90	110.0
91	84.0
92	58.0
93	46.0
94	34.0
95	29.0
96	24.0
97	25.0
98	26.0
99	29.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05972398145487754
2	0.06498481776597911
3	0.06124153039077223
4	0.07500064074288404
5	0.07985679733774705
6	0.059791428074250634
7	0.04515551167028855
8	0.056520267034655416
9	0.05473293162126835
10	0.06498481776597911
11	0.05210251346571755
12	0.06734544944403752
13	0.06259046277823417
14	0.0653894974822177
15	0.07631584982065943
16	0.07206671280015432
17	0.08360008471295394
18	0.07054916386425963
19	0.06680587648905273
20	0.07985679733774705
21	0.07894626797621022
22	0.07361798504573555
23	0.07071778041269237
24	0.05975770476456409
25	0.07513553398163024
26	0.08505018702947552
27	0.07678797615627111
28	0.09729174844569265
29	0.08875975109499586
30	0.0761472332722267
31	0.07324702863918352
32	0.07887882135683713
33	0.07665308291752493
34	0.06242184622980143
35	0.08096966655740315
36	0.08747826532690701
37	0.095099733316067
38	0.07672052953689802
39	0.05436197521471631
40	0.06036472433892196
41	0.05618303393778994
42	0.05931930173863895
43	0.038882976068590515
44	0.03851201966203848
45	0.04124360774664891
46	0.03901786930733671
47	0.031295231389117086
48	0.046470720748063944
49	0.05071985776856907
50	0.042390200275991566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2965308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.770974669263026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18151211928406	49.075297168442596
2	12.113847353754112	15.207960107796364
3	4.243618924203936	7.991282879916313
4	1.951111609595755	4.898927096913605
5	1.0642778055972202	3.340287758810097
6	0.6173742296738471	2.3251909279387695
7	0.4058298008967714	1.7832032506486302
8	0.2605968548107695	1.308633485777314
9	0.19537132475434468	1.10372836295298
>10	0.915674399366194	9.871883115292471
>50	0.03816060176155583	1.634793425601874
>100	0.01235520153411229	1.2223795616638924
>500	1.079099069454245E-4	0.0450783747846325
>1k	1.618648603838272E-4	0.19135448346044373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0116992905964574E-4	0.0	0.0	0.0	0.0
8	1.3489323874619433E-4	0.0	0.0	0.0	0.0
9	1.3489323874619433E-4	0.0	0.0	0.0	0.0
10	1.3489323874619433E-4	0.0	0.0	1.0116992905964574E-4	0.0
11	1.3489323874619433E-4	0.0	0.0	1.0116992905964574E-4	0.0
12	1.6861654843274292E-4	0.0	0.0	1.0116992905964574E-4	0.0
13	1.6861654843274292E-4	0.0	0.0	1.0116992905964574E-4	0.0
14	2.0233985811929147E-4	0.0	0.0	1.0116992905964574E-4	0.0
15	2.3606316780584006E-4	0.0	0.0	1.3489323874619433E-4	0.0
16	2.3606316780584006E-4	0.0	0.0	1.3489323874619433E-4	0.0
17	2.3606316780584006E-4	0.0	0.0	1.3489323874619433E-4	0.0
18	2.3606316780584006E-4	3.3723309686548584E-5	0.0	1.6861654843274292E-4	0.0
19	2.6978647749238867E-4	3.3723309686548584E-5	0.0	1.6861654843274292E-4	0.0
20	2.6978647749238867E-4	3.3723309686548584E-5	0.0	1.6861654843274292E-4	0.0
21	2.6978647749238867E-4	3.3723309686548584E-5	0.0	1.6861654843274292E-4	3.3723309686548584E-5
22	2.6978647749238867E-4	3.3723309686548584E-5	0.0	1.6861654843274292E-4	3.3723309686548584E-5
23	2.6978647749238867E-4	3.3723309686548584E-5	0.0	2.0233985811929147E-4	3.3723309686548584E-5
24	3.0350978717893725E-4	3.3723309686548584E-5	0.0	3.3723309686548584E-4	3.3723309686548584E-5
25	3.0350978717893725E-4	3.3723309686548584E-5	0.0	3.709564065520344E-4	3.3723309686548584E-5
26	3.3723309686548584E-4	3.3723309686548584E-5	0.0	5.058496452982288E-4	3.3723309686548584E-5
27	3.3723309686548584E-4	3.3723309686548584E-5	0.0	5.395729549847773E-4	3.3723309686548584E-5
28	3.3723309686548584E-4	3.3723309686548584E-5	0.0	0.0010116992905964575	3.3723309686548584E-5
29	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.0016187188649543318	3.3723309686548584E-5
30	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.002326908368371852	6.744661937309717E-5
31	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.005631792717653613	6.744661937309717E-5
32	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.008228487563517854	6.744661937309717E-5
33	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.01143220198373997	6.744661937309717E-5
34	4.0467971623858295E-4	3.3723309686548584E-5	0.0	0.015984848791424026	6.744661937309717E-5
35	5.058496452982288E-4	3.3723309686548584E-5	0.0	0.021582918199391093	6.744661937309717E-5
36	5.395729549847773E-4	3.3723309686548584E-5	0.0	0.027686837252656384	6.744661937309717E-5
37	5.395729549847773E-4	3.3723309686548584E-5	0.0	0.03702819403583034	6.744661937309717E-5
38	6.070195743578745E-4	3.3723309686548584E-5	0.0	0.04707774032242182	6.744661937309717E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3965	0.0	24.079765	1
GGTATCA	1710	0.0	22.513739	1
CCGTATA	60	0.007411919	18.334473	9
TCAACGC	5290	0.0	17.840511	4
TATCAAC	5290	0.0	17.83961	2
ATCAACG	5320	0.0	17.78036	3
CAACGCA	5410	0.0	17.404125	5
AACGCAG	5580	0.0	16.91189	6
GTACATG	6830	0.0	15.653854	1
TACATGG	6960	0.0	14.981415	2
GTATAGG	440	0.0	14.4994335	1
ACGCAGA	6560	0.0	14.286509	7
CGCAGAG	6690	0.0	13.844704	8
ACATGGG	7440	0.0	13.482661	3
AGAGTAC	6355	0.0	13.4680805	11
GTCTTAG	665	0.0	13.232565	1
CAGAGTA	6885	0.0	12.655022	10
ATACCGT	210	5.937691E-7	12.569877	6
GAGTACT	3520	0.0	12.376396	12
GCAGAGT	7115	0.0	12.369005	9
>>END_MODULE