FastQCFastQC Report
Mon 6 Feb 2017
SRR2077430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965308
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.10518300291234503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.026.2957781
GTATCAA46350.024.2099691
CGCATTA600.007410265518.33518212
TATCAAC64800.017.078882
TACACCG2251.8189894E-1216.6255825
ATCAACG66100.016.5432723
TCAACGC66150.016.4645214
CAACGCA67550.016.05955
AACGCAG69200.015.7065216
CGTGCCG2203.0559022E-1015.0012610
TATACTG7150.013.8489515
GTACATG67200.013.6533931
CGCACGA1801.4497218E-613.44262535
TGGACCG4100.013.4173455
TCTACAC8700.013.403653
GCACCGT4300.013.3034436
ACGCAGA81650.013.0955747
TACCGTC1851.968354E-613.08173757
CGCAGAG83400.012.8473848
GAGTACT52500.012.78224112
Produced by FastQC (version 0.11.5)