FastQCFastQC Report
Mon 6 Feb 2017
SRR2077429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425733
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.1051453805652688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60550.022.6721651
GGTATCA29400.019.8299261
ATCAACG74650.017.7706663
TCAACGC75000.017.6882514
TATCAAC77050.017.5026592
CAACGCA76500.017.283155
AACGCAG77700.017.0157326
TATACCG1906.184564E-1016.2100475
CGCGACA1004.945899E-415.40044528
ACGCAGA87150.015.0198567
GTACATG75850.014.4732981
AGAGTAC81800.014.41606411
GAGTACT50050.014.37425112
CGCAGAG90300.014.3253688
TACATGG75050.013.8359022
ATACCGT2104.0259692E-813.6182476
CAGAGTA91450.013.40005910
GTACTTT54000.013.20057514
ACATGGG76150.013.1738023
CTATACT7200.013.1390764
Produced by FastQC (version 0.11.5)