FastQCFastQC Report
Mon 6 Feb 2017
SRR2077429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425733
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40010.11679252294326499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62600.022.4575921
GGTATCA32000.021.5881941
ATCAACG85450.016.246283
TATCAAC85500.016.236782
TCAACGC85650.016.2347414
CAACGCA88200.015.8161845
AACGCAG91550.015.3326786
GTATAAT8300.014.5787831
TATATCG1100.001017634114.0018825
CTCGTAT4300.013.298676539
GTACATG74900.013.2474211
ACCGTGC3001.2732926E-1113.2010038
ACGCAGA105750.013.0445817
TACATGG75050.012.8984832
AGAGTAC100150.012.83048211
CGCAGAG109250.012.6673258
AATAGCG1404.3583688E-412.5731195
CCTAATA6650.012.5718342
TCTACAC9700.012.4746283
TCGTATG4600.012.43082740
Produced by FastQC (version 0.11.5)