FastQCFastQC Report
Mon 6 Feb 2017
SRR2077428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583450
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36400.023.752831
GGTATCA16400.021.597641
GTGTACG3100.019.1613581
CGTACAA1801.4551915E-1118.33446125
TATCAAC47250.017.7390582
TCAACGC47400.017.728994
CTAACGT750.00129188217.599044
ATCAACG48450.017.4806483
CAACGCA48500.017.4629655
AACGCAG48750.017.2828246
CGTGCCG2500.016.7210310
ACGTCGT1105.6222463E-516.00098623
AACGTCG1158.220335E-515.30499522
GTACATG65050.014.6779921
ACGCAGA56850.014.5116427
AGAGTAC52700.014.36113811
CGCACGA1707.598028E-714.235620535
TACATGG66350.014.1909092
CGCAGAG59050.014.0827588
CCGTATA1100.001018373114.000329
Produced by FastQC (version 0.11.5)