##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077428_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2583450
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.374252646654668	31.0	29.0	34.0	16.0	34.0
2	28.337831194720238	31.0	28.0	34.0	16.0	34.0
3	28.463677253285336	31.0	28.0	34.0	16.0	34.0
4	31.805100543846407	35.0	32.0	37.0	17.0	37.0
5	31.854544504441733	35.0	32.0	37.0	17.0	37.0
6	31.869822137064777	35.0	32.0	37.0	17.0	37.0
7	31.705975343049023	35.0	32.0	37.0	17.0	37.0
8	31.60092240995568	35.0	32.0	37.0	15.0	37.0
9	32.75096595637616	38.0	32.0	39.0	11.0	39.0
10	32.64268633029476	37.0	32.0	39.0	11.0	39.0
11	32.6938299560665	37.0	32.0	39.0	10.0	39.0
12	32.61065513170373	37.0	32.0	39.0	10.0	39.0
13	32.61403588225048	37.0	32.0	39.0	10.0	39.0
14	33.382317830807644	38.0	32.0	41.0	10.0	41.0
15	33.48092357119356	38.0	32.0	41.0	10.0	41.0
16	33.36531227621978	38.0	32.0	41.0	10.0	41.0
17	33.16742534208132	38.0	32.0	41.0	10.0	41.0
18	32.959095395691804	38.0	32.0	41.0	8.0	41.0
19	33.32257717393408	39.0	32.0	41.0	10.0	41.0
20	33.36105827478759	39.0	32.0	41.0	10.0	41.0
21	33.36163386169657	39.0	32.0	41.0	8.0	41.0
22	33.25481545994697	39.0	32.0	41.0	8.0	41.0
23	32.9740687839904	38.0	32.0	41.0	8.0	41.0
24	33.12429851555091	38.0	32.0	41.0	8.0	41.0
25	33.01301979910585	38.0	32.0	41.0	5.0	41.0
26	33.054717915965085	38.0	32.0	41.0	2.0	41.0
27	33.028288141825854	38.0	32.0	41.0	2.0	41.0
28	32.90594592502274	38.0	32.0	40.0	2.0	41.0
29	32.863373008960885	38.0	32.0	40.0	2.0	41.0
30	32.64128781280846	38.0	31.0	40.0	2.0	41.0
31	32.56414290967505	38.0	31.0	40.0	2.0	41.0
32	32.57701832820453	38.0	31.0	40.0	2.0	41.0
33	32.565071512899415	38.0	31.0	40.0	2.0	41.0
34	32.551971975459175	38.0	31.0	40.0	2.0	41.0
35	32.48433915887669	38.0	31.0	40.0	2.0	41.0
36	32.35281503415975	38.0	31.0	40.0	2.0	41.0
37	32.25219609436993	38.0	31.0	40.0	2.0	41.0
38	32.13918519808783	38.0	31.0	40.0	2.0	41.0
39	32.06979388027637	38.0	30.0	40.0	2.0	41.0
40	32.02459192165515	38.0	31.0	40.0	2.0	41.0
41	31.906362035262923	38.0	30.0	40.0	2.0	41.0
42	31.71343590934603	38.0	30.0	40.0	2.0	41.0
43	31.603917242447114	38.0	30.0	40.0	2.0	41.0
44	31.522832259188295	38.0	30.0	40.0	2.0	41.0
45	31.42310553716929	38.0	30.0	40.0	2.0	41.0
46	31.260842671621283	38.0	30.0	40.0	2.0	41.0
47	31.066810660163735	38.0	30.0	40.0	2.0	41.0
48	30.873558613481972	37.0	30.0	40.0	2.0	41.0
49	30.720739708529294	37.0	30.0	40.0	2.0	41.0
50	30.512674911455612	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9597639116579799
1101	2	1.0149043066425598
1101	3	1.043554897954806
1101	4	1.1287453669689675
1101	5	0.9681295543267261
1101	6	1.0559100707846483
1101	7	1.12809321967514
1101	8	1.1580817035736146
1101	9	1.4445482313364337
1101	10	1.3650804178026092
1101	11	1.485656531969454
1101	12	1.3455934154703044
1101	13	1.34637400703771
1101	14	1.6707359010172667
1101	15	1.5657291049020046
1101	16	1.517775299492186
1101	17	1.7060938279371243
1101	18	1.7690422936961312
1101	19	1.3287781090256132
1101	20	1.4261254156322707
1101	21	1.5080374248469184
1101	22	1.3757100125598285
1101	23	1.4659365120184944
1101	24	1.3324687202602234
1101	25	1.4385808783685903
1101	26	1.3569815961095344
1101	27	1.5148265881681908
1101	28	1.6784479705979578
1101	29	1.6348924469609543
1101	30	1.70339823563409
1101	31	1.6897637528435538
1101	32	1.76375657033946
1101	33	1.7328698760082375
1101	34	1.6567908901752588
1101	35	1.475350452829268
1101	36	1.5749017364167273
1101	37	1.7228792077856596
1101	38	1.7625840716278063
1101	39	1.669332645417363
1101	40	1.6439648182336235
1101	41	1.68963034072123
1101	42	1.7988123830379266
1101	43	1.9138446277138605
1101	44	1.5723366910748595
1101	45	1.6636116596831272
1101	46	1.5811784560292637
1101	47	1.6244748868656096
1101	48	1.5545050769048991
1101	49	1.6868099753099663
1101	50	1.592326134069971
1102	1	0.538744417647461
1102	2	0.5433115616800777
1102	3	0.4795594990696692
1102	4	0.5053130315995382
1102	5	0.5105944509774183
1102	6	0.5968389810462966
1102	7	0.6833051980543594
1102	8	0.6409091878368471
1102	9	0.861961087939477
1102	10	0.8875207458887004
1102	11	0.7535496440915423
1102	12	0.715726749367775
1102	13	0.6998024467534165
1102	14	1.013442924275516
1102	15	0.9745776209665493
1102	16	1.078333613060515
1102	17	1.1920545103939375
1102	18	1.3618016914396378
1102	19	1.1375958381637474
1102	20	0.9280111148747423
1102	21	0.9697586915385443
1102	22	1.067030569484622
1102	23	1.2888171093296705
1102	24	1.0250887617404416
1102	25	1.0085647948750491
1102	26	0.8776121034025479
1102	27	0.9701844560105215
1102	28	0.9805113355430564
1102	29	0.9185519746653625
1102	30	1.194852592491273
1102	31	1.1669058599572963
1102	32	1.0811493731300956
1102	33	0.9021301504138322
1102	34	0.8456719191753876
1102	35	1.0203911101761918
1102	36	1.0273291219228682
1102	37	0.9356600506020385
1102	38	1.0640107295843677
1102	39	1.1145496182722852
1102	40	1.0337617147397076
1102	41	1.0738978480012378
1102	42	1.0405474341589738
1102	43	1.0609167866570282
1102	44	0.95710196596049
1102	45	0.9334948466924899
1102	46	1.0630792214527318
1102	47	1.100786717613179
1102	48	1.127071100644553
1102	49	1.0521040125455983
1102	50	1.0184427683620427
1103	1	0.26044087089900003
1103	2	0.28059437193586945
1103	3	0.4051532562844713
1103	4	0.395556257133439
1103	5	0.3369803382641514
1103	6	0.2265157126964894
1103	7	0.5073083400653182
1103	8	0.4439832052266901
1103	9	0.5280011386784764
1103	10	0.5552476411770542
1103	11	0.5463185635289989
1103	12	0.4507903627756775
1103	13	0.36157210757328073
1103	14	0.770175126441174
1103	15	0.5894681019963244
1103	16	0.740275114207364
1103	17	0.8676327202979195
1103	18	0.6243523006436078
1103	19	0.5536057379487218
1103	20	0.6980338080699582
1103	21	0.635247145347499
1103	22	0.65150017661135
1103	23	0.9226762996405711
1103	24	0.7577253857840631
1103	25	0.5259577029870073
1103	26	0.6346814269587
1103	27	0.567267093633923
1103	28	0.6611638783003286
1103	29	0.7574976757828651
1103	30	0.7798851205595128
1103	31	0.7336207165838644
1103	32	0.6877473362560238
1103	33	0.8138251377318682
1103	34	0.7332211438178504
1103	35	0.759270296649305
1103	36	0.7473437428924257
1103	37	0.8020601058978656
1103	38	0.6461056358150543
1103	39	0.6885864074484616
1103	40	0.7087519423653355
1103	41	0.8278463853813989
1103	42	0.8537607115191008
1103	43	0.878554534748023
1103	44	0.7626341601560185
1103	45	0.7879300628582726
1103	46	0.668460874363614
1103	47	0.5366656165306551
1103	48	0.6605348849398354
1103	49	0.5377171653787265
1103	50	0.7157678450265301
1104	1	0.6575687969309918
1104	2	0.6128255079397285
1104	3	0.7068332166639166
1104	4	0.7850775456809735
1104	5	0.7450845721263626
1104	6	0.7699335565349301
1104	7	0.852837312414156
1104	8	0.8094498377861328
1104	9	1.0697624713637168
1104	10	1.0561705142896685
1104	11	1.041791221602601
1104	12	1.0292114282034248
1104	13	1.0286191224451358
1104	14	1.3338413067104256
1104	15	1.2634283211097141
1104	16	1.250829623798552
1104	17	1.5240068158660378
1104	18	1.423525263765363
1104	19	1.1333335664725936
1104	20	1.1746802710857978
1104	21	1.0445152667643995
1104	22	1.1411447006719726
1104	23	1.41611354772904
1104	24	1.1120358453331676
1104	25	1.2634448094339348
1104	26	0.9532184965476631
1104	27	0.9637263890780687
1104	28	1.1283668375694091
1104	29	1.1581520494371347
1104	30	1.36314784798072
1104	31	1.4070925989773926
1104	32	1.3473492064864487
1104	33	1.2113225927038727
1104	34	0.9970100574229761
1104	35	1.0937834606431736
1104	36	1.1892224564393317
1104	37	1.1004203686884253
1104	38	1.1844726179817044
1104	39	1.0433210842609881
1104	40	1.1234133858173898
1104	41	1.2132966089941775
1104	42	1.266825476395745
1104	43	1.19914710141067
1104	44	1.2161823085704526
1104	45	1.0992491923744936
1104	46	1.1910738836807795
1104	47	1.1141693511674795
1104	48	0.9800151903150223
1104	49	0.9861672352150421
1104	50	1.0584780109366925
1105	1	0.7922107723382297
1105	2	0.8209359354143864
1105	3	0.7190919044715613
1105	4	0.7825495751325597
1105	5	0.7834362987853645
1105	6	0.6739606427201963
1105	7	0.8551881320216204
1105	8	0.9550795277030133
1105	9	1.158970736132737
1105	10	1.1345739027128303
1105	11	1.1133590522017656
1105	12	1.1247553802110062
1105	13	1.1662711512892798
1105	14	1.4883528656278173
1105	15	1.2296517837112049
1105	16	1.1959042407671134
1105	17	1.2370475210139062
1105	18	1.573510458835294
1105	19	1.3767343611591372
1105	20	1.3123654552289992
1105	21	1.2816708252563345
1105	22	1.3329854356838169
1105	23	1.497164640891917
1105	24	1.3671180747099996
1105	25	1.3268207400662746
1105	26	1.2034865523003546
1105	27	1.2024021906346505
1105	28	1.3459356006311154
1105	29	1.1484331301401554
1105	30	1.4760345624950801
1105	31	1.4982532752438473
1105	32	1.3616284314607583
1105	33	1.4700572896880288
1105	34	1.2940132298042712
1105	35	1.2950574227582834
1105	36	1.3348016268768106
1105	37	1.2981271715386669
1105	38	1.383098088826273
1105	39	1.3166608719872102
1105	40	1.4283113310237816
1105	41	1.5174013681227407
1105	42	1.522437585533794
1105	43	1.4352519152333763
1105	44	1.2638469836200912
1105	45	1.300648636406109
1105	46	1.3544402670390454
1105	47	1.4919843903385264
1105	48	1.5854720322195455
1105	49	1.5202443483904098
1105	50	1.6299853996676887
1106	1	0.3244997415249351
1106	2	0.23453387976485374
1106	3	0.3048116511092296
1106	4	0.3459239995311485
1106	5	0.3592998617682319
1106	6	0.32355838306908424
1106	7	0.40948269885906186
1106	8	0.5335712792233736
1106	9	-0.8407592477616248
1106	10	-0.49544397602348056
1106	11	0.16875715794226664
1106	12	0.428074981569047
1106	13	0.6040588924150896
1106	14	0.7564856506354118
1106	15	0.6617348685963549
1106	16	0.7128243364953306
1106	17	0.8385416419077174
1106	18	-0.8231249688983411
1106	19	0.13012779392893492
1106	20	0.3347734555226083
1106	21	0.47168542471496977
1106	22	0.6630920408068306
1106	23	-0.9832247542672405
1106	24	0.25087995434621035
1106	25	0.5132276574356283
1106	26	0.5993221794598256
1106	27	0.6160715117050088
1106	28	0.7569053564440864
1106	29	0.7535790222490562
1106	30	-0.3038203294674986
1106	31	0.4746986001997655
1106	32	0.5516038273453603
1106	33	0.6799036545753552
1106	34	0.6420144194957089
1106	35	0.6186688655308359
1106	36	0.8186795443257608
1106	37	0.7234200544098286
1106	38	0.6202543306787263
1106	39	0.8333123815273851
1106	40	0.8014242568592032
1106	41	0.9130880455328594
1106	42	1.023198301693725
1106	43	1.0034743560741113
1106	44	0.9350774706071263
1106	45	0.8843990290376524
1106	46	0.8754039314030706
1106	47	0.9050303864977707
1106	48	0.9763145063010974
1106	49	0.989363967193448
1106	50	1.1078083310654883
1107	1	0.42246647480659405
1107	2	0.35311887386595586
1107	3	0.2661958157184401
1107	4	0.3706132635394077
1107	5	0.4007659875164755
1107	6	0.46244287408782725
1107	7	0.52201782464142
1107	8	0.32640179680890924
1107	9	0.5455221419548906
1107	10	0.7015157691535947
1107	11	0.5275890940859753
1107	12	0.5171968987633591
1107	13	0.4399867012132077
1107	14	0.8049472325277449
1107	15	0.7019359956796407
1107	16	0.48981305272371145
1107	17	0.8582167850280058
1107	18	0.9217709270458343
1107	19	0.7591650093204123
1107	20	0.834088289571298
1107	21	0.7071436154769302
1107	22	0.8255111294714155
1107	23	0.8808398501185266
1107	24	0.6640363756744563
1107	25	0.724339814102791
1107	26	0.6170585675942846
1107	27	0.6097235814943076
1107	28	0.622546371830623
1107	29	0.6172389964421185
1107	30	0.8124757986685367
1107	31	0.7567001434405043
1107	32	0.8551394600917064
1107	33	0.6884239431331451
1107	34	0.5614038513549247
1107	35	0.604964076191429
1107	36	0.5398991044315764
1107	37	0.5038950596735461
1107	38	0.6243418601879114
1107	39	0.4999402123213912
1107	40	0.6655140330069607
1107	41	0.5756787491541786
1107	42	0.642918730460579
1107	43	0.5101466961893131
1107	44	0.5995796402012843
1107	45	0.5878834948846254
1107	46	0.6642092607591046
1107	47	0.6921767908762462
1107	48	0.516039428345092
1107	49	0.5132501461317389
1107	50	0.4872126542567976
1108	1	0.21240550813960013
1108	2	0.1622028094736372
1108	3	0.1792464512063887
1108	4	0.053778410409613286
1108	5	0.18222147817562728
1108	6	-0.03596942081223986
1108	7	0.17270462924083674
1108	8	0.23504186711152997
1108	9	0.30153008596166586
1108	10	0.1684711364098419
1108	11	0.32744723258937114
1108	12	0.15534146416142391
1108	13	0.31978290419603184
1108	14	0.1355610797860507
1108	15	0.18980200826644023
1108	16	0.35841387112350986
1108	17	0.15849028617152072
1108	18	0.5000335859178193
1108	19	0.3909986343468148
1108	20	0.282951042128758
1108	21	0.3584307537530762
1108	22	0.3812524757867948
1108	23	0.05712984014259348
1108	24	0.3404606719149186
1108	25	0.3996186706066922
1108	26	0.24214304599699688
1108	27	0.3512612973049585
1108	28	0.46025599011712615
1108	29	0.4025281251642454
1108	30	0.4658243136708009
1108	31	0.6631824257158456
1108	32	0.631806977097753
1108	33	0.6234739546438348
1108	34	0.5691471543397384
1108	35	0.4377624660575208
1108	36	0.5212681982394187
1108	37	0.41728660125588846
1108	38	0.37337660647715865
1108	39	0.5635844706195314
1108	40	0.6324928957032192
1108	41	0.5360836292133655
1108	42	0.5765588377988848
1108	43	0.6665645215544949
1108	44	0.6280106040506013
1108	45	0.4462131616849163
1108	46	0.5137124047926704
1108	47	0.6020674230102436
1108	48	0.6041242181438626
1108	49	0.4599025792430673
1108	50	0.5345007086477054
1109	1	0.006378298566172447
1109	2	-0.13705207891392845
1109	3	-0.08146904757089857
1109	4	0.05506419310874833
1109	5	-0.08820593242919017
1109	6	0.028027246128949912
1109	7	-0.07359291408166158
1109	8	0.0069205829580418765
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2211	8	0.04904719096727206
2211	9	0.36617675444760067
2211	10	0.0451120563875449
2211	11	0.15380799187650496
2211	12	0.1563682858879929
2211	13	0.09609929017869234
2211	14	0.3378089156135857
2211	15	0.050145845075228124
2211	16	0.09448040950067593
2211	17	0.3683016639066139
2211	18	0.5781048474514847
2211	19	0.3505944371431866
2211	20	0.3484160868839652
2211	21	0.20344535287306797
2211	22	0.1478603602465114
2211	23	0.4585916160967187
2211	24	0.45812998475109623
2211	25	0.3538793280936403
2211	26	0.2788952435089769
2211	27	0.23538279260623796
2211	28	0.2835696825375962
2211	29	0.3176237334213212
2211	30	0.29757290293017746
2211	31	0.21728295945969478
2211	32	0.2128226115654499
2211	33	0.33153112714617805
2211	34	0.17275510118608395
2211	35	0.08128433698397686
2211	36	0.2853772656050495
2211	37	0.15317338850104534
2211	38	0.19140707385302846
2211	39	0.07649876919586518
2211	40	0.10568472250097827
2211	41	0.25717220018716347
2211	42	0.2262757429959521
2211	43	-0.010005979726550152
2211	44	0.07815189126274191
2211	45	0.023201300638596933
2211	46	-0.1513199789290134
2211	47	-0.04055198786585734
2211	48	0.08279089868729272
2211	49	0.08111173106291858
2211	50	0.22206907449470847
2212	1	0.11857538021241254
2212	2	0.024154343082798846
2212	3	-0.15914143582890006
2212	4	0.016684582837566353
2212	5	0.17521228608086048
2212	6	0.16894234448607648
2212	7	0.11698362113182625
2212	8	0.12678605752398298
2212	9	-0.09057956462724803
2212	10	0.2325198655693157
2212	11	0.17024245798954496
2212	12	0.2114700131582481
2212	13	0.05050359434160612
2212	14	0.03560639794331166
2212	15	0.004509347720613732
2212	16	-0.09569353796008784
2212	17	0.23294557829107276
2212	18	0.4164972580322157
2212	19	0.2164035417671215
2212	20	0.026446377376935004
2212	21	0.1487330453961988
2212	22	0.18830994964061887
2212	23	0.45098935458534584
2212	24	0.4083005597678451
2212	25	0.08368253178306162
2212	26	0.2403056737369056
2212	27	0.1829879872532416
2212	28	0.017289350475827803
2212	29	0.05901655400314354
2212	30	0.2772924424459333
2212	31	0.16420584200385946
2212	32	0.14075136465362448
2212	33	0.2959523843998113
2212	34	0.0020892739795499438
2212	35	0.2718874827087632
2212	36	0.13896186246650188
2212	37	-0.9800719106435842
2212	38	-0.3408354828639375
2212	39	0.01779381056577023
2212	40	0.08133410001606123
2212	41	-0.666308405160521
2212	42	-0.22686855855157262
2212	43	-0.0567058440645134
2212	44	0.05318407060117991
2212	45	0.07013020712362561
2212	46	0.1735988738935923
2212	47	0.17008759156798803
2212	48	0.10392951498266001
2212	49	0.23976331673446083
2212	50	0.29223703291856395
2213	1	-0.8843924243834742
2213	2	-0.7490148731982877
2213	3	-0.7634056247828411
2213	4	-0.8749415891962471
2213	5	-0.7454516206779687
2213	6	-0.8522564643084358
2213	7	-0.7691581351598415
2213	8	-0.7655468472959264
2213	9	-0.9272500258922456
2213	10	-1.0951506621172342
2213	11	-0.993539581460869
2213	12	-0.8602525641249699
2213	13	-0.9370811779541661
2213	14	-3.0384083765256413
2213	15	-1.846539169706137
2213	16	-1.4038549912611629
2213	17	-0.9360907981225424
2213	18	-1.0950083305601588
2213	19	-1.0998688109994248
2213	20	-1.2787768404353983
2213	21	-1.1400853562746605
2213	22	-1.2266910995958042
2213	23	-1.8128437118063871
2213	24	-1.5829489765418678
2213	25	-1.3498544880854482
2213	26	-1.3856501009634314
2213	27	-1.371837650569514
2213	28	-1.2525311799839933
2213	29	-1.5847013522326563
2213	30	-1.3644382952883483
2213	31	-1.4300352911391059
2213	32	-1.2584259169593786
2213	33	-1.365208058011305
2213	34	-1.3785516012461017
2213	35	-1.3138889176950457
2213	36	-1.2062250930378475
2213	37	-1.408965584678601
2213	38	-1.2454172813790443
2213	39	-1.3501374531500154
2213	40	-1.530553502765379
2213	41	-1.768657854362754
2213	42	-1.488233382631261
2213	43	-1.5872454715547661
2213	44	-1.5980986092538494
2213	45	-1.5337169017927152
2213	46	-1.7198175052627889
2213	47	-1.5798020222862412
2213	48	-1.655307422770882
2213	49	-1.8469841020225495
2213	50	-1.6914139898644045
2214	1	-0.5928482360224123
2214	2	-0.5412231383857389
2214	3	-0.6413688501503572
2214	4	-0.8621771086763665
2214	5	-0.7227112763100472
2214	6	-0.6412499492278236
2214	7	-0.8129447267737397
2214	8	-0.5823203016142422
2214	9	-0.7543704099867092
2214	10	-0.9937716399484202
2214	11	-0.7430519639746187
2214	12	-0.7224767220536279
2214	13	-0.6946525626991225
2214	14	-1.2396060698948759
2214	15	-1.0696267543211988
2214	16	-0.6657073461273768
2214	17	-1.2219335022761122
2214	18	-1.278874309043207
2214	19	-0.8833833263301614
2214	20	-0.9063161667083577
2214	21	-1.0385670282102737
2214	22	-0.8454555287661591
2214	23	-0.7143130891533858
2214	24	-0.7330879357344244
2214	25	-0.858629166546514
2214	26	-0.9514807261089473
2214	27	-0.8713391166235098
2214	28	-0.8026521605950521
2214	29	-0.809840044370187
2214	30	-0.5399152477526172
2214	31	-0.5737884181035611
2214	32	-0.5892481209698026
2214	33	-0.6909883881063053
2214	34	-0.8301358997100436
2214	35	-0.7382194232436383
2214	36	-0.7383280117337421
2214	37	-0.88113798265843
2214	38	-0.8381360058467884
2214	39	-0.7484834111582188
2214	40	-0.7910921967894744
2214	41	-0.660740513108852
2214	42	-0.6038580255818182
2214	43	-0.726745021059493
2214	44	-0.6801127376202949
2214	45	-0.7486122377751094
2214	46	-0.6405899945598179
2214	47	-0.6720981751259245
2214	48	-0.745440336212507
2214	49	-0.705542765765415
2214	50	-0.7089654082639143
2215	1	-0.3219185784789609
2215	2	-0.6149148792012262
2215	3	-0.4119398830902874
2215	4	-0.12241672082273425
2215	5	-0.3414283735321284
2215	6	-0.3808710799285251
2215	7	-0.5104199796171862
2215	8	-0.4942326654407765
2215	9	-0.5734499127188855
2215	10	-0.966959209974739
2215	11	-0.578040473387766
2215	12	-0.6147321151799368
2215	13	-0.5910748143450348
2215	14	-0.5591963693613948
2215	15	-0.7712722348089116
2215	16	-0.6276983790149018
2215	17	-0.5018162342768449
2215	18	-0.6834505910793709
2215	19	-0.8100726709493173
2215	20	-0.7735634832941471
2215	21	-0.7023021820688413
2215	22	-1.0749155666426091
2215	23	-0.9517195819058379
2215	24	-0.6834328405136034
2215	25	-0.6791910841324835
2215	26	-0.6716520872764846
2215	27	-0.7006781654896699
2215	28	-0.6164891181706551
2215	29	-0.6534467746833599
2215	30	-0.4366948137869002
2215	31	-0.7736602416273115
2215	32	-0.8446886022005948
2215	33	-0.7577528342577722
2215	34	-0.7421714237605421
2215	35	-0.5611522687277208
2215	36	-0.6781758470898573
2215	37	-0.7196139104498513
2215	38	-0.656266351290629
2215	39	-0.8905588368751971
2215	40	-0.7037184406983776
2215	41	-0.7194016529136746
2215	42	-0.7415273849277888
2215	43	-0.8305640361745077
2215	44	-0.7204763485283223
2215	45	-0.8526103780182943
2215	46	-0.6842665780577697
2215	47	-0.8000360050427773
2215	48	-0.8916442498625905
2215	49	-0.8806144626100831
2215	50	-0.8134180059900551
2216	1	-1.584357186209047
2216	2	-1.9560911326844028
2216	3	-1.7659086643136774
2216	4	-3.428165383571894
2216	5	-2.1888868719622288
2216	6	-2.001906450839332
2216	7	-1.8916742141350014
2216	8	-1.8740395846099283
2216	9	-1.9140382767252824
2216	10	-2.3429000308582637
2216	11	-2.042672198856561
2216	12	-2.1710745990261593
2216	13	-2.265700397291493
2216	14	-2.164163639998815
2216	15	-2.252049830683667
2216	16	-2.443774651742892
2216	17	-2.2077607723090757
2216	18	-1.9966902311750125
2216	19	-2.2294817233927446
2216	20	-2.429590141313831
2216	21	-2.457947107369968
2216	22	-2.6986359843341283
2216	23	-4.537606807304773
2216	24	-3.0975442969768245
2216	25	-2.915700602405831
2216	26	-2.942777223374218
2216	27	-2.8471311883751795
2216	28	-3.295341969170991
2216	29	-2.8449550670885166
2216	30	-2.687785572849755
2216	31	-2.4312989822256945
2216	32	-2.5835958132054664
2216	33	-2.6750515480188675
2216	34	-2.9208225996713075
2216	35	-3.1626449280180857
2216	36	-3.0225644024898735
2216	37	-2.8279529776827914
2216	38	-2.967172887408921
2216	39	-3.155039240119926
2216	40	-3.1663545054769457
2216	41	-3.1585247748967866
2216	42	-3.248178708469993
2216	43	-3.228562575677781
2216	44	-3.286569097979317
2216	45	-3.2546859178616465
2216	46	-3.361484938136119
2216	47	-3.3287345021020123
2216	48	-3.2400689433296357
2216	49	-3.651316476461073
2216	50	-3.6487601813920207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	171453.0
3	8311.0
4	16097.0
5	12402.0
6	9876.0
7	8531.0
8	7419.0
9	6692.0
10	6467.0
11	6403.0
12	6462.0
13	6537.0
14	7068.0
15	7348.0
16	8115.0
17	8673.0
18	9744.0
19	10739.0
20	13299.0
21	17366.0
22	21513.0
23	24846.0
24	26924.0
25	28874.0
26	31009.0
27	35354.0
28	40056.0
29	45024.0
30	51696.0
31	61679.0
32	73853.0
33	90385.0
34	112725.0
35	141890.0
36	184195.0
37	247364.0
38	379754.0
39	637307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12585656364405	17.268966019424997	12.56555432817368	25.039623088757267
2	15.93329764677903	19.692741866138235	38.63072085548572	25.74323963159701
3	18.84664053757906	24.546972515654794	28.8258139582624	27.780572988503742
4	12.895808234779139	15.951717806383137	36.93134585065214	34.22112810818558
5	14.292288936417982	37.35009256809343	33.984906306523385	14.3727121889652
6	32.98873319846791	36.18107755226616	17.193133715464942	13.637055533800984
7	28.865658786779886	31.536961318820566	21.527690104430896	18.069689789968653
8	26.76413039436829	34.55064230473688	19.61120888382123	19.074018417073592
9	27.118973599076373	15.403449970623468	18.389631575298615	39.087944855001545
10	14.926547855736558	28.439751501792003	33.41804334968271	23.215657292788723
11	35.42307632724512	22.81587426995709	23.178959272513204	18.58209013028458
12	23.746248069564245	24.44370332238306	29.869297830746056	21.940750777306636
13	28.436778216633684	21.564286967606392	25.468012904610234	24.53092191114969
14	22.84384388106552	20.57996904394118	26.284761655389964	30.291425419603335
15	24.059111720423317	28.512557250533334	23.16971647742306	24.25861455162029
16	24.288703625800583	27.56688413258061	24.53812703511141	23.606285206507398
17	23.174687418283245	27.47457216739465	25.88918481787454	23.46155559644756
18	23.341733671825914	27.076256119167567	26.46416505464352	23.117845154363
19	24.10355687714948	26.741360738202392	26.41952240570377	22.73555997894436
20	24.254125518655723	26.195489094623024	26.392433969814437	23.157951416906812
21	24.374232026118474	26.098599711481995	26.34078748990884	23.186380772490693
22	24.30588804031032	25.652122040369203	26.30315947027625	23.738830449044226
23	24.23475830354639	25.872807812623467	26.202827009514778	23.689606874315366
24	23.78779891806389	26.058261256547137	26.105049357961125	24.048890467427853
25	24.013213961430356	25.86228535834813	26.30605629569028	23.818444384531237
26	23.689564867042705	26.25368034463522	26.461794148639434	23.59496063968264
27	23.810115951326168	26.19050386065387	26.245899556369572	23.75348063165039
28	23.970865318935182	26.064799442382135	26.26542098947725	23.698914249205437
29	23.98687259943762	26.284167697854382	26.103125486698648	23.625834216009352
30	24.05300814645805	26.29419443809583	26.100236685015243	23.552560730430873
31	24.284889756581293	25.844218224639366	25.990408900044937	23.880483118734407
32	23.725276606822028	26.204298184517143	26.202283822113202	23.868141386547624
33	24.09103234553554	25.92763896959132	26.121557234166183	23.859771450706955
34	24.20753900671586	25.920879200898288	26.147775252837263	23.723806539548587
35	24.144083837132456	26.07231154116432	26.07405479072095	23.709549830982272
36	24.148351094905216	26.104745493230354	25.918555044343233	23.828348367521198
37	24.133094667999472	25.828393425854895	26.228777770028906	23.809734136116727
38	24.201551961101806	26.04245270593777	26.116750364515624	23.6392449684448
39	24.21841414038917	26.241501723086884	25.93294174967099	23.607142386852953
40	24.183574347835517	25.959384382756024	26.22756272212634	23.62947854728212
41	23.996031025237148	26.040819867491706	26.158440635980956	23.80470847129019
42	24.21564906192387	26.039281453824348	26.031612623359724	23.713456860892062
43	24.119809665132692	25.853122449074423	26.10126409737314	23.925803788419746
44	24.19558258233722	26.0601233165754	25.839558343423846	23.904735757663527
45	24.062813908838358	26.102345220918245	25.751154564936385	24.08368630530701
46	24.18729379327437	25.98553261357828	25.775298564104155	24.051875029043202
47	24.145529564322786	26.06350210619204	25.817008521956165	23.973959807529013
48	23.911092901613333	26.340950729410434	25.831209573204568	23.91674679577166
49	24.265366796697556	25.939438627499655	25.886034698225203	23.90915987757759
50	24.22799796624005	25.945193034875576	25.804394269072016	24.022414729812358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1534.0
1	1264.5
2	995.0
3	948.0
4	901.0
5	877.5
6	854.0
7	830.0
8	806.0
9	905.5
10	1005.0
11	1245.5
12	1486.0
13	2433.5
14	3381.0
15	4834.0
16	6287.0
17	7029.5
18	7772.0
19	8585.0
20	9398.0
21	11038.0
22	12678.0
23	15889.5
24	19101.0
25	23658.0
26	28215.0
27	33355.5
28	38496.0
29	44331.5
30	50167.0
31	56408.5
32	62650.0
33	70807.0
34	78964.0
35	86937.0
36	94910.0
37	102409.5
38	109909.0
39	117400.5
40	124892.0
41	133889.0
42	142886.0
43	148651.0
44	154416.0
45	161740.5
46	169065.0
47	174868.0
48	180671.0
49	182597.0
50	184523.0
51	187045.0
52	189567.0
53	185671.5
54	181776.0
55	176021.5
56	170267.0
57	159904.5
58	149542.0
59	136698.5
60	123855.0
61	109227.0
62	94599.0
63	81006.0
64	67413.0
65	56863.0
66	46313.0
67	38353.0
68	30393.0
69	24416.5
70	18440.0
71	14529.0
72	10618.0
73	8621.5
74	6625.0
75	5104.5
76	3584.0
77	2762.5
78	1941.0
79	1540.0
80	1139.0
81	868.0
82	597.0
83	482.0
84	367.0
85	275.0
86	183.0
87	132.0
88	81.0
89	73.0
90	65.0
91	52.0
92	39.0
93	32.5
94	26.0
95	17.5
96	9.0
97	13.0
98	17.0
99	24.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06278426135593876
2	0.06626797499467765
3	0.06398420716483771
4	0.07586754146586928
5	0.08155760707580949
6	0.06259072170934216
7	0.04892682265962182
8	0.05798447812034296
9	0.05639745301825079
10	0.06584218777216513
11	0.0545781803402427
12	0.06886140625907217
13	0.06723567322766069
14	0.06680988600514816
15	0.07869322030617973
16	0.07339023398943273
17	0.08225434980355725
18	0.06998393620933248
19	0.06653893049991291
20	0.07807389343707058
21	0.07671911591089434
22	0.07269349126168495
23	0.06897753004703013
24	0.06181656312295574
25	0.07501596702084422
26	0.0839962066229267
27	0.07826743308366718
28	0.09564729334804235
29	0.0884089105653293
30	0.07586754146586928
31	0.0731579864135168
32	0.07695136348681027
33	0.07548046217267608
34	0.06390679130619907
35	0.07989316611507867
36	0.08600901894753141
37	0.09406026824595018
38	0.0751707987381215
39	0.05635874508893147
40	0.0613907759004432
41	0.055081383421393876
42	0.060771449031334066
43	0.0407981575025644
44	0.03835955795544717
45	0.0422690588166986
46	0.04219164295805996
47	0.03201145754707852
48	0.04486249008109311
49	0.04811395614391608
50	0.040294954421413225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2583450.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.06342491837261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77290295761811	50.30752476188375
2	11.22975248565619	14.163733054621746
3	3.9095025663822467	7.396398646896955
4	1.7362696671351006	4.379804471656889
5	0.9596395384262281	3.0259077990122085
6	0.5685087350469633	2.1511264756843897
7	0.3599071997263715	1.5887886467268033
8	0.27651183477000935	1.3950226664847951
9	0.1974910402685155	1.1209015251022347
>10	0.9189912308144718	10.08205126849739
>50	0.051835700160535916	2.2258803360225734
>100	0.01782099374474107	1.7413411288579643
>500	7.42328786225923E-4	0.29956928082179507
>1k	1.2372146436808685E-4	0.12194993773026917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	3.870792931932106E-5	0.0	3.870792931932106E-5	0.0
17	0.0	3.870792931932106E-5	0.0	3.870792931932106E-5	0.0
18	0.0	3.870792931932106E-5	0.0	3.870792931932106E-5	3.870792931932106E-5
19	0.0	7.741585863864212E-5	0.0	7.741585863864212E-5	3.870792931932106E-5
20	0.0	7.741585863864212E-5	0.0	7.741585863864212E-5	3.870792931932106E-5
21	0.0	7.741585863864212E-5	0.0	7.741585863864212E-5	3.870792931932106E-5
22	0.0	7.741585863864212E-5	0.0	1.1612378795796319E-4	3.870792931932106E-5
23	0.0	7.741585863864212E-5	0.0	1.5483171727728425E-4	3.870792931932106E-5
24	0.0	7.741585863864212E-5	0.0	2.3224757591592637E-4	3.870792931932106E-5
25	0.0	7.741585863864212E-5	0.0	3.096634345545685E-4	3.870792931932106E-5
26	0.0	7.741585863864212E-5	0.0	4.6449515183185274E-4	7.741585863864212E-5
27	0.0	7.741585863864212E-5	0.0	5.419110104704949E-4	7.741585863864212E-5
28	0.0	7.741585863864212E-5	0.0	0.0010451140916216688	7.741585863864212E-5
29	0.0	7.741585863864212E-5	0.0	0.0017031488900501267	7.741585863864212E-5
30	0.0	7.741585863864212E-5	0.0	0.002516015405755869	7.741585863864212E-5
31	0.0	7.741585863864212E-5	0.0	0.004412703942402601	7.741585863864212E-5
32	0.0	7.741585863864212E-5	0.0	0.0064642241963266175	7.741585863864212E-5
33	0.0	7.741585863864212E-5	0.0	0.009406026824595019	7.741585863864212E-5
34	0.0	7.741585863864212E-5	0.0	0.013354235615165766	7.741585863864212E-5
35	0.0	7.741585863864212E-5	0.0	0.01741856819369448	7.741585863864212E-5
36	0.0	7.741585863864212E-5	0.0	0.022373183146567575	7.741585863864212E-5
37	0.0	7.741585863864212E-5	0.0	0.029340610424045364	7.741585863864212E-5
38	0.0	7.741585863864212E-5	0.0	0.0371209042172289	7.741585863864212E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3640	0.0	23.75283	1
GGTATCA	1640	0.0	21.59764	1
GTGTACG	310	0.0	19.161358	1
CGTACAA	180	1.4551915E-11	18.334461	25
TATCAAC	4725	0.0	17.739058	2
TCAACGC	4740	0.0	17.72899	4
CTAACGT	75	0.001291882	17.59904	4
ATCAACG	4845	0.0	17.480648	3
CAACGCA	4850	0.0	17.462965	5
AACGCAG	4875	0.0	17.282824	6
CGTGCCG	250	0.0	16.72103	10
ACGTCGT	110	5.6222463E-5	16.000986	23
AACGTCG	115	8.220335E-5	15.304995	22
GTACATG	6505	0.0	14.677992	1
ACGCAGA	5685	0.0	14.511642	7
AGAGTAC	5270	0.0	14.361138	11
CGCACGA	170	7.598028E-7	14.2356205	35
TACATGG	6635	0.0	14.190909	2
CGCAGAG	5905	0.0	14.082758	8
CCGTATA	110	0.0010183731	14.00032	9
>>END_MODULE