FastQCFastQC Report
Mon 6 Feb 2017
SRR2077428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583450
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45000.023.6648561
GGTATCA20700.023.1716611
CGTGCCG1801.4551915E-1118.33537310
TGCGATA2150.017.39762313
TATCAAC61800.017.1598222
TCAACGC62250.016.8593834
ATCAACG63050.016.680363
CAACGCA63000.016.5555085
TATATCG800.00198666616.5031175
AACGCAG65200.016.130326
GCGATAC2600.016.07902314
GCACCGT4050.015.7545366
CGCACGA2403.6379788E-1215.58084635
TATGCGA900.004357566614.66829912
GGTACGA1351.9559771E-514.668015518
GTACATG64850.014.6230621
ACACCGT3200.014.4388286
TACCGAT2753.6379788E-1214.4010457
TACCGTA950.00623936513.8957467
TATACTG4950.013.780385
Produced by FastQC (version 0.11.5)