FastQCFastQC Report
Mon 6 Feb 2017
SRR2077427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715061
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.025.9824261
GTATCAA52750.025.2319011
TATCAAC69900.018.662812
ATCAACG70600.018.384043
TCAACGC71350.018.1310884
CAACGCA72500.017.843255
GCGATAC2800.017.28642514
AACGCAG75300.017.2082796
TGCGATA3250.016.24638413
ACGCAGA88900.014.6252347
GTACATG98100.014.554351
GGTGCGA3500.014.45734611
GAGTACT43650.014.414865512
CGTGCCG4350.014.16111910
CGCAGAG91450.014.0249698
TACATGG99600.014.003272
AGAGTAC89600.013.97117911
TACCGAT4450.013.8425197
CGAATCG3550.013.63362837
GTACTTT47550.013.27919814
Produced by FastQC (version 0.11.5)