##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077427_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3715061
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.694629509448163	31.0	30.0	34.0	25.0	34.0
2	29.73003431168425	31.0	30.0	34.0	25.0	34.0
3	29.80192734385788	31.0	30.0	34.0	25.0	34.0
4	33.20003655390854	37.0	35.0	37.0	26.0	37.0
5	33.264734280271576	37.0	35.0	37.0	27.0	37.0
6	33.34195643086345	37.0	35.0	37.0	27.0	37.0
7	33.21021350658845	37.0	35.0	37.0	26.0	37.0
8	33.14588697197704	37.0	35.0	37.0	26.0	37.0
9	34.45429644358464	39.0	35.0	39.0	26.0	39.0
10	34.35434869037144	39.0	35.0	39.0	25.0	39.0
11	34.423495065087764	39.0	35.0	39.0	26.0	39.0
12	34.3479248389192	39.0	35.0	39.0	25.0	39.0
13	34.36163686141358	39.0	35.0	39.0	25.0	39.0
14	35.30258022681189	40.0	36.0	41.0	25.0	41.0
15	35.390102342868666	40.0	36.0	41.0	25.0	41.0
16	35.29431091441029	40.0	36.0	41.0	25.0	41.0
17	35.12541193805431	40.0	35.0	41.0	24.0	41.0
18	34.95150469938448	39.0	34.0	41.0	22.0	41.0
19	35.286243213772266	40.0	35.0	41.0	25.0	41.0
20	35.31566291912838	40.0	35.0	41.0	25.0	41.0
21	35.31604945383131	40.0	35.0	41.0	25.0	41.0
22	35.234744193971515	40.0	35.0	41.0	24.0	41.0
23	34.98499136353347	39.0	35.0	41.0	23.0	41.0
24	35.13027780701313	40.0	35.0	41.0	24.0	41.0
25	35.04202891957898	39.0	35.0	41.0	24.0	41.0
26	35.07836102825768	39.0	35.0	41.0	24.0	41.0
27	35.06381671794891	39.0	35.0	41.0	24.0	41.0
28	34.95598726373537	39.0	35.0	41.0	24.0	41.0
29	34.9206163774969	39.0	35.0	41.0	23.0	41.0
30	34.703698808714044	39.0	34.0	41.0	22.0	41.0
31	34.61869751263842	39.0	34.0	41.0	21.0	41.0
32	34.63666491613462	39.0	34.0	41.0	22.0	41.0
33	34.629094111779054	39.0	34.0	41.0	22.0	41.0
34	34.62309609451904	39.0	34.0	41.0	22.0	41.0
35	34.55931975275776	39.0	34.0	41.0	21.0	41.0
36	34.4389604908237	39.0	34.0	41.0	19.0	41.0
37	34.349238680064744	39.0	34.0	41.0	19.0	41.0
38	34.25783560485279	39.0	34.0	41.0	18.0	41.0
39	34.19388295373885	39.0	34.0	41.0	17.0	41.0
40	34.15076414626839	39.0	34.0	41.0	16.0	41.0
41	34.031519805462146	39.0	33.0	41.0	15.0	41.0
42	33.858951441174185	39.0	33.0	41.0	15.0	41.0
43	33.75955684173154	39.0	33.0	41.0	12.0	41.0
44	33.697439153758175	38.0	33.0	41.0	9.0	41.0
45	33.61170489528974	38.0	33.0	41.0	8.0	41.0
46	33.462151765475724	38.0	33.0	40.0	2.0	41.0
47	33.28598669039351	38.0	33.0	40.0	2.0	41.0
48	33.105559235770286	38.0	33.0	40.0	2.0	41.0
49	32.97697830533603	38.0	33.0	40.0	2.0	41.0
50	32.78029055242969	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8793160370926643
1101	2	0.874525242088172
1101	3	0.9851312313898326
1101	4	0.9460261437386066
1101	5	0.7744491043803094
1101	6	0.8462976259402666
1101	7	0.8966390525990775
1101	8	0.9409522826032699
1101	9	1.2603833560628246
1101	10	1.1619862025056804
1101	11	1.1196230710952975
1101	12	1.190535427250694
1101	13	1.1198436298388401
1101	14	1.4674624412062371
1101	15	1.2994361244868529
1101	16	1.2000191470947783
1101	17	1.4409132078067088
1101	18	1.5347622389459445
1101	19	1.237175308777914
1101	20	1.292571008103046
1101	21	1.2955732430838935
1101	22	1.1107893202694186
1101	23	1.2938030753780012
1101	24	1.2118198748615896
1101	25	1.2160740901761073
1101	26	1.1469977608145427
1101	27	1.2348547308612652
1101	28	1.3959589255989187
1101	29	1.3865932547849695
1101	30	1.4519768056423388
1101	31	1.4858138492434492
1101	32	1.3877961790509445
1101	33	1.384978471046665
1101	34	1.2812773305198988
1101	35	1.298017258610166
1101	36	1.3878703887345765
1101	37	1.4781969777894304
1101	38	1.3986599161504003
1101	39	1.3128350680864855
1101	40	1.3014525783811663
1101	41	1.3390509517953078
1101	42	1.5016007790725112
1101	43	1.4737017969025672
1101	44	1.2834990247487852
1101	45	1.4365354768484693
1101	46	1.30337790694783
1101	47	1.2994322043793005
1101	48	1.3198560893354028
1101	49	1.3814769555659936
1101	50	1.3849614725532646
1102	1	0.5917711897117499
1102	2	0.644184677977119
1102	3	0.5985170016608485
1102	4	0.6089579205228404
1102	5	0.5685932783605523
1102	6	0.6412496047447007
1102	7	0.6805754107157611
1102	8	0.6661057296419628
1102	9	0.9207043253182192
1102	10	0.9765945813763537
1102	11	0.7530011138176036
1102	12	0.7500323086328251
1102	13	0.7669049534575407
1102	14	1.1779581082592827
1102	15	1.0790649261648326
1102	16	1.1549087535417613
1102	17	1.192062128709729
1102	18	1.1745362081461437
1102	19	1.0820127542710978
1102	20	0.952540097908205
1102	21	0.9171482831015609
1102	22	1.0515101901706174
1102	23	1.2364558324569828
1102	24	1.052449394097664
1102	25	0.9856603903361787
1102	26	0.9529214788694063
1102	27	0.9113773339412461
1102	28	0.9207704286071419
1102	29	0.7656532536810303
1102	30	1.0270170992891892
1102	31	1.088914528163805
1102	32	1.0673007879817789
1102	33	0.8880291968842755
1102	34	0.8331772035673097
1102	35	0.9481395194701321
1102	36	1.006040615593328
1102	37	1.024975141944637
1102	38	1.1536394933430927
1102	39	1.1657469740311797
1102	40	1.0109257251493915
1102	41	1.1083408452655306
1102	42	1.1284965123616928
1102	43	1.2248921153879735
1102	44	1.1202636656937486
1102	45	1.1867628875676886
1102	46	1.1278405663285227
1102	47	1.1707152534492309
1102	48	1.10731565769661
1102	49	1.0957911356178798
1102	50	1.1594647015646942
1103	1	0.5498032283425793
1103	2	0.5598104960676871
1103	3	0.6119871977313025
1103	4	0.5601955855821501
1103	5	0.5331540357852518
1103	6	0.5789123242303518
1103	7	0.6666665146378463
1103	8	0.6437142601647992
1103	9	0.8099861689637322
1103	10	0.7723616366572372
1103	11	0.7845908714932435
1103	12	0.8015060246121237
1103	13	0.7742807562883911
1103	14	1.005349984662331
1103	15	0.9149174019697028
1103	16	0.9597275358838928
1103	17	1.0787770839139696
1103	18	0.8539087284415672
1103	19	0.8138174366315383
1103	20	0.901139018198208
1103	21	0.9182498653650129
1103	22	0.9323994927674804
1103	23	1.0220363122225962
1103	24	0.9187024545193765
1103	25	0.6900099532299606
1103	26	0.7970097275282484
1103	27	0.7541143173997042
1103	28	0.858158710498266
1103	29	0.9489097458443183
1103	30	1.022787070872198
1103	31	0.9596519795870222
1103	32	0.8807703058424323
1103	33	0.930769503570744
1103	34	0.8290828849307843
1103	35	0.7912671178614161
1103	36	0.9093813753239459
1103	37	0.8543872018712548
1103	38	0.8529250671424933
1103	39	0.8819236002087152
1103	40	0.8811908034487104
1103	41	0.9674522547889524
1103	42	0.924583275741071
1103	43	0.9439612385305267
1103	44	0.9285514572391875
1103	45	0.9314895011983495
1103	46	0.9673082055480364
1103	47	0.8626380460458947
1103	48	0.9432263239020955
1103	49	0.8608911037420199
1103	50	0.994479656090725
1104	1	0.7663993218870537
1104	2	0.712596378086932
1104	3	0.6878591200595707
1104	4	0.7377929010559328
1104	5	0.7194657950821224
1104	6	0.7010085145979517
1104	7	0.7225066276469789
1104	8	0.7748204146774071
1104	9	0.9929554983241076
1104	10	1.1074735870666785
1104	11	1.0223497211850798
1104	12	0.9076838010956365
1104	13	0.8726392052295324
1104	14	1.13181155739575
1104	15	1.1582642872509865
1104	16	1.1437442332263004
1104	17	1.27582573058654
1104	18	1.349522731057192
1104	19	1.1525283777112492
1104	20	1.172327958545118
1104	21	1.022227149029483
1104	22	1.04779999749816
1104	23	1.2719589771110762
1104	24	1.1220775262729674
1104	25	1.2434152071475992
1104	26	1.0897967908390598
1104	27	1.0863638568522944
1104	28	1.1801484859097329
1104	29	1.1960835222245123
1104	30	1.3857417673534798
1104	31	1.3887423209161227
1104	32	1.2951973638028846
1104	33	1.2862552126574585
1104	34	1.2056095685468478
1104	35	1.3133870985856362
1104	36	1.3415737093339715
1104	37	1.264240391021211
1104	38	1.2956740346229125
1104	39	1.2556286751392918
1104	40	1.302971746912398
1104	41	1.4538734398547248
1104	42	1.4589103089075621
1104	43	1.399169344099164
1104	44	1.4317651132329345
1104	45	1.31864418935465
1104	46	1.415964256055986
1104	47	1.492861604652191
1104	48	1.5344814758058263
1104	49	1.3832369776863658
1104	50	1.51198042483513
1105	1	0.6816694287179281
1105	2	0.7778378315767469
1105	3	0.7163595364346946
1105	4	0.7172521023049754
1105	5	0.6993360014583132
1105	6	0.622326520197177
1105	7	0.6560098772965262
1105	8	0.6539784953604055
1105	9	0.8182210927546976
1105	10	0.923135863961349
1105	11	0.9223841051943538
1105	12	0.8834497269431978
1105	13	0.8785361028942233
1105	14	1.1155353375849515
1105	15	0.9555687091628187
1105	16	1.0767515195905943
1105	17	1.020537365766586
1105	18	1.2166295771619033
1105	19	1.051732415948834
1105	20	1.0764494711529906
1105	21	1.0326313174293773
1105	22	1.1021060536522995
1105	23	1.2960053418236228
1105	24	1.13672319064176
1105	25	1.135183963865103
1105	26	1.098587716025385
1105	27	1.0771697811609897
1105	28	1.1235222705472125
1105	29	1.04760455377685
1105	30	1.2612852791550466
1105	31	1.3310371369456462
1105	32	1.1333079905456316
1105	33	1.1742197674727066
1105	34	1.0756989268963508
1105	35	1.0894728197641825
1105	36	1.0743124958589618
1105	37	1.0741085215329704
1105	38	1.1025396155884408
1105	39	1.0487943523640126
1105	40	1.16329877170174
1105	41	1.2344914383206742
1105	42	1.3006148260366501
1105	43	1.2236592545163916
1105	44	1.2038401681551818
1105	45	1.0985283416926421
1105	46	1.015093515084594
1105	47	1.14195587855248
1105	48	1.1899706945354112
1105	49	1.1712923415517338
1105	50	1.1813053200600834
1106	1	0.38260535019908914
1106	2	0.38487559485300693
1106	3	0.40060765773994333
1106	4	0.48481125152068216
1106	5	0.41426716559512755
1106	6	0.4361205687197156
1106	7	0.39114011362150336
1106	8	0.5093889471737398
1106	9	-0.7385545446921
1106	10	-0.27283234686560576
1106	11	0.24902581166310966
1106	12	0.5470260417248269
1106	13	0.5689411146844563
1106	14	0.7752479624855511
1106	15	0.6847678124457559
1106	16	0.7165978020227186
1106	17	0.8134315064952915
1106	18	-0.7903122767023447
1106	19	0.16474401680005002
1106	20	0.3983857412393945
1106	21	0.47381855032095643
1106	22	0.741369379365068
1106	23	-0.8018529781928692
1106	24	0.28633049800767196
1106	25	0.5034449799704817
1106	26	0.5694225201103293
1106	27	0.6472119809897663
1106	28	0.7622112335739644
1106	29	0.6370699252133107
1106	30	-0.2960734400152205
1106	31	0.5418032418906691
1106	32	0.6150075278272453
1106	33	0.6394429490494673
1106	34	0.6803097039079091
1106	35	0.656049155327743
1106	36	0.6535728745995755
1106	37	0.6698314117720088
1106	38	0.7276820382526878
1106	39	0.7349590368559547
1106	40	0.7230941337050822
1106	41	0.752824214604388
1106	42	0.9479125416332081
1106	43	0.7969898752602944
1106	44	0.8750179522977461
1106	45	0.758564039744563
1106	46	0.8850884789810607
1106	47	0.874333711634165
1106	48	0.9804197234018304
1106	49	0.9384272267799858
1106	50	0.9356339080807174
1107	1	0.1850986235695551
1107	2	0.32404568374045084
1107	3	0.2548214664617241
1107	4	0.20285760350706994
1107	5	0.12129290429490425
1107	6	0.24477771801463177
1107	7	0.3362747254689893
1107	8	0.05189373101634942
1107	9	0.3754113936678536
1107	10	0.4183281519852997
1107	11	0.36162209520508526
1107	12	0.3398423260719312
1107	13	0.2747384196911469
1107	14	0.5203212047708092
1107	15	0.43176293136415467
1107	16	0.3251850888653607
1107	17	0.5862584710349026
1107	18	0.5788935218750666
1107	19	0.42573736545676155
1107	20	0.42500150820700355
1107	21	0.39185201273702575
1107	22	0.4774037338105259
1107	23	0.6613315544944456
1107	24	0.551384214617471
1107	25	0.49026386130233135
1107	26	0.4740801099317693
1107	27	0.4709479039861719
1107	28	0.4926563158947488
1107	29	0.374968331054923
1107	30	0.629932056080392
1107	31	0.5761536708597959
1107	32	0.4758305219867438
1107	33	0.4988498074475771
1107	34	0.4096439039452662
1107	35	0.37756594134097554
1107	36	0.4185741161865195
1107	37	0.3302215260847774
1107	38	0.3690123596454171
1107	39	0.33852144550947827
1107	40	0.3944284252939596
1107	41	0.368217280426407
1107	42	0.4551100513338824
1107	43	0.4881565674822852
1107	44	0.4306936142840385
1107	45	0.45335931157790554
1107	46	0.5294846723634876
1107	47	0.6100577536599303
1107	48	0.42476034200984003
1107	49	0.4338209376937172
1107	50	0.5643687216358728
1108	1	0.1202989707056652
1108	2	0.1824814116680855
1108	3	0.14552998199095413
1108	4	0.07819183662024898
1108	5	0.16056206896428193
1108	6	0.07688369837289599
1108	7	0.1553007525911525
1108	8	0.21853976666614017
1108	9	0.30821876577465446
1108	10	0.09328421126327413
1108	11	0.15931870094276235
1108	12	0.14296265996366486
1108	13	0.2064359119951007
1108	14	0.130951688849251
1108	15	0.19221712664597135
1108	16	0.336826150271591
1108	17	0.22450574982302385
1108	18	0.5471470683985444
1108	19	0.3040586678470589
1108	20	0.1316150725007148
1108	21	0.25306192338303646
1108	22	0.2494559146377071
1108	23	0.18093134478765904
1108	24	0.34131236091999284
1108	25	0.3726320754634713
1108	26	0.28512813498713996
1108	27	0.26970082589446776
1108	28	0.35437018675362
1108	29	0.2634621636677821
1108	30	0.44269004385584765
1108	31	0.6395349210471366
1108	32	0.4158588006662285
1108	33	0.47292583023175894
1108	34	0.25045680167879425
1108	35	0.2949969030781219
1108	36	0.2510525796240515
1108	37	0.26338070972151684
1108	38	0.175584355885384
1108	39	0.25855446891751654
1108	40	0.369512490101215
1108	41	0.3158794710743891
1108	42	0.34433270805720895
1108	43	0.31557837476301387
1108	44	0.3146314374251915
1108	45	0.21027921911284864
1108	46	0.30100273683525813
1108	47	0.3337111496580505
1108	48	0.44014444341181047
1108	49	0.28581474206558966
1108	50	0.36161243804726695
1109	1	0.08990000746478444
1109	2	-0.002403893893291098
1109	3	0.07048483588338073
1109	4	0.11175773495104835
1109	5	-0.04501068050436885
1109	6	0.07449363165261502
1109	7	0.06079075131732026
1109	8	0.03339135556015549
1109	9	0.16464695270552454
1109	10	-0.04282202729912399
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2211	6	-0.29467772622815147
2211	7	-0.2616588949100489
2211	8	-0.2793809786140926
2211	9	-0.265556563795478
2211	10	-0.3659370321795379
2211	11	-0.17078998380505794
2211	12	-0.2606036156070459
2211	13	-0.27860395453998166
2211	14	-0.17939871745869596
2211	15	-0.30176686161687627
2211	16	-0.29989453988823556
2211	17	-0.07311294013756964
2211	18	0.09227689268500683
2211	19	-0.256086779253323
2211	20	-0.18724886404312713
2211	21	-0.26227199453571615
2211	22	-0.2793662781034243
2211	23	-0.1270739264369638
2211	24	-0.13248934585624994
2211	25	-0.11177286993471824
2211	26	-0.2025149287066057
2211	27	-0.23329978627457137
2211	28	-0.25577596604243524
2211	29	-0.3264177854916781
2211	30	-0.10033045311906363
2211	31	-0.07916421066607171
2211	32	-0.10680363510594759
2211	33	-0.17409242397786073
2211	34	-0.3353556464298748
2211	35	-0.33823349238846845
2211	36	-0.32592616909727923
2211	37	-0.3324643005327417
2211	38	-0.237826813631564
2211	39	-0.37877587203188057
2211	40	-0.34511176150266465
2211	41	-0.2635132546614045
2211	42	-0.2594091246865915
2211	43	-0.35209799308164946
2211	44	-0.35910518722593565
2211	45	-0.4250004463276653
2211	46	-0.4807574778461543
2211	47	-0.35579179988634735
2211	48	-0.2527203115047598
2211	49	-0.33147211397465526
2211	50	-0.22160931630750724
2212	1	-0.44636646709175665
2212	2	-0.3908375027978188
2212	3	-0.5522147372471444
2212	4	-0.5160635478768398
2212	5	-0.4227506499356437
2212	6	-0.3365414814980312
2212	7	-0.44696355944866895
2212	8	-0.3487462905894958
2212	9	-0.5829821518566334
2212	10	-0.43387451772799324
2212	11	-0.45701433177311657
2212	12	-0.43847866518414946
2212	13	-0.516117460458382
2212	14	-0.5305732330137971
2212	15	-0.49604040823058426
2212	16	-0.5728500167690243
2212	17	-0.22251863845365705
2212	18	-0.10222313206485012
2212	19	-0.47870107811348106
2212	20	-0.501310063671454
2212	21	-0.4129076383558328
2212	22	-0.3119772428822998
2212	23	-0.10419025951713934
2212	24	-0.2685515255140203
2212	25	-0.3421153150772298
2212	26	-0.387865112933099
2212	27	-0.48139296389637565
2212	28	-0.5690386548249506
2212	29	-0.4776295010093108
2212	30	-0.30557274094446996
2212	31	-0.426703489749201
2212	32	-0.47675599480205477
2212	33	-0.5014945478781527
2212	34	-0.5425062233462228
2212	35	-0.5410895933636368
2212	36	-0.4910974815876088
2212	37	-1.7208799350242643
2212	38	-1.0980217009864006
2212	39	-0.6951196304645606
2212	40	-0.6474545601892743
2212	41	-1.5808265175860399
2212	42	-1.0776953561986033
2212	43	-0.7773958072610299
2212	44	-0.7240487302832719
2212	45	-0.6123201235777458
2212	46	-0.5819357154612277
2212	47	-0.46786271602224616
2212	48	-0.5585215451295795
2212	49	-0.6063427196922291
2212	50	-0.4397105621760531
2213	1	-0.882606466633117
2213	2	-0.8400487767891249
2213	3	-0.8164708550483191
2213	4	-0.8659305129926409
2213	5	-0.7637556894597282
2213	6	-0.7658018524501955
2213	7	-0.7966824824232788
2213	8	-0.8500934203276742
2213	9	-0.964824666788644
2213	10	-0.9915315570571579
2213	11	-1.0497447878217372
2213	12	-0.9464491728486593
2213	13	-1.0434077362813738
2213	14	-2.8669265304778193
2213	15	-1.7478347398946639
2213	16	-1.5346275245395944
2213	17	-1.2176654062022294
2213	18	-1.292997944859323
2213	19	-1.2233158735370395
2213	20	-1.3455002785338124
2213	21	-1.3332767320775574
2213	22	-1.4057127910072822
2213	23	-1.921112473553947
2213	24	-1.522454218531358
2213	25	-1.3853292878666181
2213	26	-1.506209516880439
2213	27	-1.4626117370970064
2213	28	-1.2760480291949179
2213	29	-1.59716854352601
2213	30	-1.3322072480089275
2213	31	-1.3186446703790011
2213	32	-1.3546125392799055
2213	33	-1.3351842214520957
2213	34	-1.3509879451880025
2213	35	-1.3879769043307206
2213	36	-1.3122972714740655
2213	37	-1.4008703346795244
2213	38	-1.285626596517261
2213	39	-1.2729665960963246
2213	40	-1.4532667460102928
2213	41	-1.6361415469627616
2213	42	-1.3972442283790514
2213	43	-1.512931958686103
2213	44	-1.4262314769899191
2213	45	-1.4301436090929087
2213	46	-1.54037209305217
2213	47	-1.4715874180747512
2213	48	-1.5956928426069723
2213	49	-1.8614751060336054
2213	50	-1.6565189049926836
2214	1	-0.699221932882736
2214	2	-0.7055812378535329
2214	3	-0.7590651801802757
2214	4	-0.929455859526378
2214	5	-0.7453867834730019
2214	6	-0.7223894261089754
2214	7	-0.861917806521916
2214	8	-0.7463988447791365
2214	9	-1.0445565744230905
2214	10	-1.1629691134939861
2214	11	-0.8649744682380458
2214	12	-0.9450579178798861
2214	13	-1.0106406330147877
2214	14	-1.4071986839673585
2214	15	-1.3301861306841758
2214	16	-1.0145211789460902
2214	17	-1.7098294732840884
2214	18	-1.671108312012862
2214	19	-1.1491037199544039
2214	20	-1.2137950020981094
2214	21	-1.2467626891995351
2214	22	-1.1350255534094202
2214	23	-0.8686804537440338
2214	24	-0.9328111901663263
2214	25	-1.060562715222531
2214	26	-1.1824208140197356
2214	27	-1.0304057438554608
2214	28	-1.0439951717461895
2214	29	-0.8905370422366872
2214	30	-0.820376111638943
2214	31	-0.7555441127599138
2214	32	-0.8848236362721167
2214	33	-0.8984866716247808
2214	34	-1.1091071676863962
2214	35	-0.9830015619861143
2214	36	-1.0178160946092092
2214	37	-1.1340355211886788
2214	38	-0.9759320884601763
2214	39	-0.9873374476988985
2214	40	-1.0952371242417343
2214	41	-1.02012151829053
2214	42	-0.9108728484812687
2214	43	-0.9648804254663403
2214	44	-1.0097527391267391
2214	45	-1.0119288279979628
2214	46	-0.9118404861038059
2214	47	-0.8727777114065915
2214	48	-1.0760372352303875
2214	49	-1.0558799477312277
2214	50	-0.9991667578333754
2215	1	-0.7013882233221409
2215	2	-0.868554060117269
2215	3	-0.7781273736446153
2215	4	-0.7455798615463252
2215	5	-0.7120116877255853
2215	6	-0.7167021529350848
2215	7	-0.8292980530279976
2215	8	-0.847725463959101
2215	9	-1.0021070087065027
2215	10	-1.2978579094150504
2215	11	-0.9914957212859221
2215	12	-1.051400358343777
2215	13	-1.0814007838858544
2215	14	-1.1529893341944373
2215	15	-1.1803161607476298
2215	16	-1.0551723655560963
2215	17	-1.0658319932848102
2215	18	-1.0762501881632645
2215	19	-1.259709819606286
2215	20	-1.2599173756580555
2215	21	-1.2642643574114985
2215	22	-1.5044483383611293
2215	23	-1.4287244057148811
2215	24	-1.3256263369278543
2215	25	-1.145226734316637
2215	26	-1.2039541544079029
2215	27	-1.1936843547187692
2215	28	-1.2102082455932859
2215	29	-1.272856682067399
2215	30	-1.0587847936884387
2215	31	-1.1370581857796225
2215	32	-1.295867446232478
2215	33	-1.3342596610112807
2215	34	-1.2346977860022363
2215	35	-1.2969671691302835
2215	36	-1.2465193743934435
2215	37	-1.223658886354663
2215	38	-1.1966958307160809
2215	39	-1.2543883834054341
2215	40	-1.2777077667267775
2215	41	-1.4455184211460406
2215	42	-1.2622265264157448
2215	43	-1.5149737192268162
2215	44	-1.5178286944488164
2215	45	-1.4589077605506588
2215	46	-1.3985491271950607
2215	47	-1.5698373815803883
2215	48	-1.6787142151115049
2215	49	-1.7430558526642557
2215	50	-1.7129899706807734
2216	1	-1.2012386185154504
2216	2	-1.5708994971070567
2216	3	-1.3367809049414063
2216	4	-2.422759251194332
2216	5	-1.6441266747941867
2216	6	-1.496815832233775
2216	7	-1.479480949992773
2216	8	-1.4531732924631449
2216	9	-1.4842524892038114
2216	10	-1.8525336575344937
2216	11	-1.7200753025196818
2216	12	-1.78319489089872
2216	13	-1.802935479861155
2216	14	-1.7420755617743922
2216	15	-1.9022994421560568
2216	16	-2.048886444818656
2216	17	-1.8612389985044402
2216	18	-1.7115770988367274
2216	19	-1.9695690053541384
2216	20	-2.0970126762097863
2216	21	-2.2011733389157158
2216	22	-2.3932555835082
2216	23	-4.1513109659619865
2216	24	-2.8604220742085857
2216	25	-2.486558904057027
2216	26	-2.6161020901541647
2216	27	-2.5146042207359613
2216	28	-2.963473459549789
2216	29	-2.5971603900021734
2216	30	-2.3500675149693393
2216	31	-2.2551017212872324
2216	32	-2.3344324867949737
2216	33	-2.437731306702318
2216	34	-2.516893796286297
2216	35	-2.756454976211323
2216	36	-2.6100441450566514
2216	37	-2.4749048170657524
2216	38	-2.6100729938476555
2216	39	-2.784501159477827
2216	40	-2.906905485507906
2216	41	-2.9383931614684755
2216	42	-3.30341023628419
2216	43	-3.2194784013738484
2216	44	-3.2243403454319974
2216	45	-3.1109585295015556
2216	46	-3.2250997059595754
2216	47	-3.2879613678360826
2216	48	-3.2668901686831298
2216	49	-3.537876702180391
2216	50	-3.6260820705038164
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147681.0
3	7607.0
4	14909.0
5	11790.0
6	9570.0
7	8386.0
8	7487.0
9	6911.0
10	6614.0
11	6550.0
12	6755.0
13	6948.0
14	7399.0
15	7880.0
16	8571.0
17	9419.0
18	10341.0
19	11834.0
20	15086.0
21	20007.0
22	25197.0
23	28981.0
24	31326.0
25	32955.0
26	35937.0
27	41886.0
28	47657.0
29	54495.0
30	63061.0
31	75842.0
32	92433.0
33	114270.0
34	146217.0
35	189706.0
36	251399.0
37	349622.0
38	566199.0
39	1236133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07905965525433	17.455018711117265	12.805654867556965	24.66026676607144
2	15.983977693104567	19.664550794139053	39.00886554719178	25.34260596556459
3	18.821608512568407	24.755529770341006	28.942484786094948	27.480376930995643
4	12.777274477049891	16.362814005103765	37.125248608559815	33.734662909286534
5	14.319527774956567	37.34788018940942	34.060505517437015	14.272086518196994
6	32.695384938596476	36.27253224375023	17.502703532571	13.52937928508229
7	28.786684074232067	31.698950375512208	21.545147641145622	17.969217909110103
8	26.784055969369398	34.395408641467235	19.743444994839596	19.077090394323772
9	27.056084973004246	15.693493466587988	18.66045460384903	38.58996695655874
10	14.8872082026872	28.63121280103603	33.40886814412505	23.07271085215172
11	35.1340789690911	22.9090567300993	23.413167476104025	18.543696824705574
12	23.835294162012747	24.539247685026186	29.8676268922421	21.75783126071897
13	28.2974593210437	21.800946973726987	25.55511951105795	24.346474194171364
14	22.740637627694422	20.863638004252575	26.494583003598045	29.901141364454958
15	23.927005907111162	28.633595320861087	23.37441300642002	24.064985765607723
16	24.090673880092243	27.78047898129938	24.749477216104037	23.379369922504342
17	22.938773398967	27.596826005760906	26.0431539937865	23.421246601485592
18	22.962096803954065	27.168424140408266	26.783662780533835	23.085816275103834
19	23.901240833011446	26.835781544864844	26.729950066073965	22.533027556049742
20	24.04310625136549	26.359031442843918	26.791139204700816	22.80672310108978
21	24.286843415350067	26.155683809220044	26.641475094340432	22.915997681089458
22	24.12811840371348	25.827542698412525	26.580926839202267	23.46341205867173
23	24.003604156902043	25.993082604690255	26.421944951756014	23.581368286651692
24	23.553352511808338	26.147779477882583	26.463898419620758	23.83496959068832
25	23.803343410923507	25.988464513063285	26.58808247514881	23.620109600864396
26	23.511952469767582	26.409231895775314	26.70221430039708	23.37660133406002
27	23.700236980549384	26.29872587597542	26.513993759359543	23.48704338411565
28	23.78962649776892	26.209740324300263	26.50507067747614	23.495562500454675
29	23.801722228778065	26.30696106530104	26.393902312200712	23.497414393720188
30	23.803769299192233	26.327877631659053	26.492362215202732	23.375990853945986
31	24.080149988282166	25.934611789488425	26.292557962874668	23.692680259354738
32	23.646697935689545	26.289065962085477	26.49218620145487	23.572049900770107
33	23.840944178099075	26.147782125925435	26.35483021975491	23.65644347622058
34	24.1228912475917	26.08443871154026	26.345759304394857	23.446910736473185
35	23.932353375380792	26.149543264594033	26.392350638308876	23.525752721716295
36	23.96992484444071	26.171166947934243	26.287715180474642	23.571193027150404
37	23.960168008042054	25.91604070495696	26.47217868623617	23.651612600764814
38	23.950646616922135	26.094731190703435	26.410746068266867	23.543876124107562
39	24.049276049297596	26.251373567180902	26.212186503490475	23.487163880031027
40	23.999790990449394	26.058831340335693	26.505616188974802	23.435761480240114
41	23.834268338993642	26.097969560919903	26.434598531102964	23.633163568983488
42	24.041510032614717	26.084475054952662	26.344310039402156	23.529704873030465
43	23.88380024262139	25.965120146057536	26.400492244059343	23.75058736726173
44	23.984339804379502	26.146274025418332	26.14837440243601	23.72101176776616
45	23.918189221154407	26.158584794490153	26.104161684838225	23.81906429951722
46	24.018004165785893	26.04367477787636	26.01111863603499	23.927202420302756
47	23.913645796226245	26.121263476015727	26.101473028581985	23.863617699176043
48	23.787721045366244	26.39445809179674	26.122872489684596	23.694948373152425
49	24.014316743129456	26.043841659406404	26.1229661727893	23.818875424674836
50	24.14322676269191	26.06111053064144	26.091755738840348	23.703906967826303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2278.0
1	1849.5
2	1421.0
3	1411.5
4	1402.0
5	1365.0
6	1328.0
7	1316.5
8	1305.0
9	1390.5
10	1476.0
11	1851.5
12	2227.0
13	3880.5
14	5534.0
15	7947.0
16	10360.0
17	11493.5
18	12627.0
19	13727.0
20	14827.0
21	17502.5
22	20178.0
23	25132.5
24	30087.0
25	36992.5
26	43898.0
27	51819.0
28	59740.0
29	68596.0
30	77452.0
31	86686.5
32	95921.0
33	106272.5
34	116624.0
35	128918.5
36	141213.0
37	151159.0
38	161105.0
39	172303.5
40	183502.0
41	195283.0
42	207064.0
43	214652.5
44	222241.0
45	231486.0
46	240731.0
47	249547.5
48	258364.0
49	261182.5
50	264001.0
51	265949.5
52	267898.0
53	262948.5
54	257999.0
55	249985.5
56	241972.0
57	225861.5
58	209751.0
59	191637.0
60	173523.0
61	152403.5
62	131284.0
63	112576.0
64	93868.0
65	78889.0
66	63910.0
67	52382.0
68	40854.0
69	32727.0
70	24600.0
71	19247.5
72	13895.0
73	11164.5
74	8434.0
75	6419.5
76	4405.0
77	3463.5
78	2522.0
79	1998.5
80	1475.0
81	1106.5
82	738.0
83	588.5
84	439.0
85	327.5
86	216.0
87	177.5
88	139.0
89	112.0
90	85.0
91	61.5
92	38.0
93	33.5
94	29.0
95	28.0
96	27.0
97	23.0
98	19.0
99	27.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06177556707682593
2	0.0669437190937107
3	0.06295993524736201
4	0.07806062942169725
5	0.08349795602279478
6	0.06261000828788545
7	0.046647955444069425
8	0.058491637149430384
9	0.055719138932039065
10	0.06524791921317039
11	0.053511907341494525
12	0.06998539189531477
13	0.06438656054368959
14	0.0669437190937107
15	0.07873356588222913
16	0.07332315673955286
17	0.08465540673490961
18	0.07079291564795302
19	0.06877410626635741
20	0.08139839426593534
21	0.08002560388645032
22	0.07388842336639963
23	0.07216570602743805
24	0.05970292277838776
25	0.07709160091853134
26	0.08705106053440306
27	0.0791911626753908
28	0.09754886931870028
29	0.09041574283706243
30	0.07695701362642499
31	0.07458827728535278
32	0.08005252134487159
33	0.07752228025327175
34	0.0640635510426343
35	0.08250201006120761
36	0.08863919058125828
37	0.09614916148079398
38	0.0778991246711696
39	0.056553580143098595
40	0.062233163869987604
41	0.05574605639046034
42	0.0624485035373578
43	0.03919181946137627
44	0.038814975043478424
45	0.04212582242929524
46	0.039730168629801776
47	0.030901242267623603
48	0.04530208252300568
49	0.05184302491937548
50	0.04287951126509094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3715061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.68661585616543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67477572164692	42.897469413344744
2	13.142548883160204	14.900132398211575
3	4.661580933975622	7.927477430601029
4	2.2200013555943214	5.033774561789673
5	1.2310243136945918	3.4891301190002513
6	0.7294978880191846	2.481165992761651
7	0.46990702198307477	1.8646207190288473
8	0.340031876349794	1.5420205082793534
9	0.23725896389231166	1.2104466970135768
>10	1.1851153189393917	11.990608255893576
>50	0.07338530483464249	2.881533035505589
>100	0.033963168092757	3.2272970526208367
>500	5.742630471412386E-4	0.2475386543425382
>1k	3.349867699944221E-4	0.306785161606693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.6917458421274912E-5	2.6917458421274912E-5	0.0	0.0	0.0
6	2.6917458421274912E-5	2.6917458421274912E-5	0.0	0.0	2.6917458421274912E-5
7	5.3834916842549824E-5	2.6917458421274912E-5	0.0	0.0	2.6917458421274912E-5
8	5.3834916842549824E-5	2.6917458421274912E-5	0.0	0.0	2.6917458421274912E-5
9	5.3834916842549824E-5	2.6917458421274912E-5	0.0	2.6917458421274912E-5	5.3834916842549824E-5
10	5.3834916842549824E-5	2.6917458421274912E-5	0.0	2.6917458421274912E-5	5.3834916842549824E-5
11	5.3834916842549824E-5	2.6917458421274912E-5	0.0	2.6917458421274912E-5	5.3834916842549824E-5
12	5.3834916842549824E-5	2.6917458421274912E-5	0.0	2.6917458421274912E-5	5.3834916842549824E-5
13	5.3834916842549824E-5	2.6917458421274912E-5	0.0	5.3834916842549824E-5	5.3834916842549824E-5
14	5.3834916842549824E-5	2.6917458421274912E-5	0.0	8.075237526382474E-5	1.0766983368509965E-4
15	5.3834916842549824E-5	2.6917458421274912E-5	0.0	8.075237526382474E-5	1.0766983368509965E-4
16	5.3834916842549824E-5	2.6917458421274912E-5	0.0	8.075237526382474E-5	1.3458729210637457E-4
17	5.3834916842549824E-5	2.6917458421274912E-5	0.0	8.075237526382474E-5	1.3458729210637457E-4
18	5.3834916842549824E-5	5.3834916842549824E-5	0.0	8.075237526382474E-5	1.3458729210637457E-4
19	5.3834916842549824E-5	5.3834916842549824E-5	0.0	8.075237526382474E-5	1.3458729210637457E-4
20	5.3834916842549824E-5	8.075237526382474E-5	0.0	1.0766983368509965E-4	1.3458729210637457E-4
21	5.3834916842549824E-5	8.075237526382474E-5	0.0	1.0766983368509965E-4	1.3458729210637457E-4
22	5.3834916842549824E-5	8.075237526382474E-5	0.0	1.6150475052764947E-4	1.3458729210637457E-4
23	5.3834916842549824E-5	8.075237526382474E-5	0.0	2.4225712579147422E-4	1.3458729210637457E-4
24	5.3834916842549824E-5	8.075237526382474E-5	0.0	2.9609204263402405E-4	1.3458729210637457E-4
25	5.3834916842549824E-5	8.075237526382474E-5	0.0	2.9609204263402405E-4	1.3458729210637457E-4
26	5.3834916842549824E-5	8.075237526382474E-5	0.0	3.2300950105529895E-4	1.3458729210637457E-4
27	5.3834916842549824E-5	1.0766983368509965E-4	0.0	4.306793347403986E-4	1.3458729210637457E-4
28	5.3834916842549824E-5	1.0766983368509965E-4	0.0	6.998539189531477E-4	1.3458729210637457E-4
29	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.0015881300468552199	1.3458729210637457E-4
30	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.0023687363410721924	1.3458729210637457E-4
31	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.004199123513718887	1.3458729210637457E-4
32	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.006110263061629405	1.3458729210637457E-4
33	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.00899043111270582	1.3458729210637457E-4
34	5.3834916842549824E-5	1.0766983368509965E-4	0.0	0.012570453082735385	1.3458729210637457E-4
35	5.3834916842549824E-5	1.3458729210637457E-4	0.0	0.016850328971718096	1.3458729210637457E-4
36	5.3834916842549824E-5	1.3458729210637457E-4	0.0	0.02185697623807523	1.3458729210637457E-4
37	5.3834916842549824E-5	1.6150475052764947E-4	0.0	0.028532505926551408	1.3458729210637457E-4
38	5.3834916842549824E-5	1.6150475052764947E-4	0.0	0.034723521363444636	1.3458729210637457E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	25.982426	1
GTATCAA	5275	0.0	25.231901	1
TATCAAC	6990	0.0	18.66281	2
ATCAACG	7060	0.0	18.38404	3
TCAACGC	7135	0.0	18.131088	4
CAACGCA	7250	0.0	17.84325	5
GCGATAC	280	0.0	17.286425	14
AACGCAG	7530	0.0	17.208279	6
TGCGATA	325	0.0	16.246384	13
ACGCAGA	8890	0.0	14.625234	7
GTACATG	9810	0.0	14.55435	1
GGTGCGA	350	0.0	14.457346	11
GAGTACT	4365	0.0	14.4148655	12
CGTGCCG	435	0.0	14.161119	10
CGCAGAG	9145	0.0	14.024969	8
TACATGG	9960	0.0	14.00327	2
AGAGTAC	8960	0.0	13.971179	11
TACCGAT	445	0.0	13.842519	7
CGAATCG	355	0.0	13.633628	37
GTACTTT	4755	0.0	13.279198	14
>>END_MODULE