FastQCFastQC Report
Mon 6 Feb 2017
SRR2077427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715061
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.1195404328488819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.026.7546771
GTATCAA62400.024.6825471
GCGATAC3850.018.8599914
TGCGATA3950.017.82547413
TATCAAC90050.016.7605272
GCACCGT4900.016.612966
ATCAACG91200.016.5978783
TCAACGC91450.016.4805494
CAACGCA91500.016.3998475
GTGCGAT4850.015.87847112
AACGCAG95250.015.7760086
CGCACGA3400.015.52778235
ACCGTGC6950.015.5116698
CGTGCCG3300.015.33440710
GCGCGTT1901.0797521E-815.05105136
ACCGTCG1655.422171E-714.6674978
TATACTG9800.014.5936465
TCATTCG5200.014.38562324
GTACATG92850.014.2893311
GGTGCGA5150.014.09845411
Produced by FastQC (version 0.11.5)