##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077427_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3715061 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.696266629269346 31.0 31.0 34.0 27.0 34.0 2 30.645958168654566 31.0 31.0 34.0 27.0 34.0 3 30.8249258895076 31.0 31.0 34.0 27.0 34.0 4 34.295513855627135 37.0 35.0 37.0 30.0 37.0 5 33.93826453993622 37.0 35.0 37.0 30.0 37.0 6 33.88819106873346 37.0 35.0 37.0 29.0 37.0 7 33.713653423187395 37.0 35.0 37.0 28.0 37.0 8 33.686401111583365 37.0 35.0 37.0 28.0 37.0 9 35.17019720537563 39.0 35.0 39.0 28.0 39.0 10 35.03068051910857 39.0 35.0 39.0 28.0 39.0 11 34.973743096008384 39.0 35.0 39.0 27.0 39.0 12 34.895922031966634 39.0 35.0 39.0 27.0 39.0 13 34.86638092887304 39.0 35.0 39.0 27.0 39.0 14 36.03441235554409 40.0 36.0 41.0 27.0 41.0 15 36.03597276060878 40.0 36.0 41.0 27.0 41.0 16 35.97441926256393 40.0 36.0 41.0 27.0 41.0 17 35.947385251547686 40.0 36.0 41.0 27.0 41.0 18 35.93797275468693 40.0 36.0 41.0 27.0 41.0 19 35.97306881367493 40.0 36.0 41.0 27.0 41.0 20 35.96551658236567 40.0 36.0 41.0 27.0 41.0 21 35.90889759279861 40.0 36.0 41.0 27.0 41.0 22 35.90792237328001 40.0 36.0 41.0 27.0 41.0 23 35.84217325099103 40.0 36.0 41.0 27.0 41.0 24 35.82428229307675 40.0 36.0 41.0 27.0 41.0 25 35.802716294564206 40.0 36.0 41.0 27.0 41.0 26 35.72123741709759 40.0 36.0 41.0 27.0 41.0 27 35.66663293011878 40.0 36.0 41.0 27.0 41.0 28 35.59935005104896 40.0 35.0 41.0 26.0 41.0 29 35.519940587785776 40.0 35.0 41.0 26.0 41.0 30 35.391494244643624 40.0 35.0 41.0 25.0 41.0 31 35.31517867405138 39.0 35.0 41.0 25.0 41.0 32 35.229736739181405 39.0 35.0 41.0 25.0 41.0 33 35.13564837831734 39.0 35.0 41.0 24.0 41.0 34 35.10729918028264 39.0 35.0 41.0 24.0 41.0 35 35.041892717239364 39.0 35.0 41.0 24.0 41.0 36 34.93493350445659 39.0 35.0 41.0 24.0 41.0 37 34.84482408229636 39.0 35.0 41.0 24.0 41.0 38 34.769039054809596 39.0 34.0 41.0 23.0 41.0 39 34.67679292479989 39.0 34.0 41.0 23.0 41.0 40 34.58044915009471 39.0 34.0 41.0 22.0 41.0 41 34.52150664551672 39.0 34.0 41.0 22.0 41.0 42 34.40720865686997 39.0 34.0 41.0 21.0 41.0 43 34.508695012006534 39.0 34.0 41.0 21.0 41.0 44 34.441259780122046 39.0 34.0 41.0 19.0 41.0 45 34.37268647809552 39.0 34.0 41.0 19.0 41.0 46 34.221072278490176 39.0 34.0 41.0 15.0 41.0 47 34.06872753906329 39.0 34.0 41.0 13.0 41.0 48 33.90829141163496 39.0 34.0 41.0 7.0 41.0 49 33.7519736553451 39.0 34.0 41.0 2.0 41.0 50 33.610238701329536 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6173485878547993 1101 2 0.8023719809100207 1101 3 0.6763179790906229 1101 4 0.688148104533397 1101 5 0.6826628182477847 1101 6 0.6636316294710198 1101 7 0.6813799630870676 1101 8 0.5795824626039661 1101 9 0.7556713588729664 1101 10 0.8375765814909641 1101 11 0.8716806647122013 1101 12 0.7699650229212267 1101 13 0.8192947068228591 1101 14 0.8691287448433869 1101 15 0.8531836670372215 1101 16 0.9217085276682866 1101 17 0.9896743403881345 1101 18 0.9363403653148197 1101 19 0.9330861244629176 1101 20 0.9616375957937464 1101 21 1.019952551092338 1101 22 1.0032186989463625 1101 23 1.061089763556467 1101 24 1.0278842093569125 1101 25 1.039758882387062 1101 26 1.0451571567976998 1101 27 1.0953261603216404 1101 28 1.08669835758689 1101 29 1.1654109633947556 1101 30 1.2308181380732535 1101 31 1.248497373068254 1101 32 1.1446613480425256 1101 33 1.208050510917218 1101 34 1.2346277712429057 1101 35 1.1747091776756307 1101 36 1.1439591374430051 1101 37 1.1656715028095377 1101 38 1.0876142888724303 1101 39 1.065138753924174 1101 40 1.1529408868359354 1101 41 1.1178267832824886 1101 42 1.1584768909140806 1101 43 1.1756495489172352 1101 44 1.1929044990595798 1101 45 1.1738176522121933 1101 46 1.12759847786932 1101 47 1.129993696515129 1101 48 1.2276209363779955 1101 49 1.2407630156201677 1101 50 1.2423811781836136 1102 1 0.6254454030007963 1102 2 0.6818983925675788 1102 3 0.5766331526844866 1102 4 0.6120712154237182 1102 5 0.6048828325989462 1102 6 0.5827559326117182 1102 7 0.63493132368761 1102 8 0.6633234241318107 1102 9 0.7395861902468113 1102 10 0.6729889014114789 1102 11 0.7115023791240844 1102 12 0.6900207716655018 1102 13 0.7323848983556687 1102 14 0.7713418595974844 1102 15 0.7485758401343148 1102 16 0.7870466079244025 1102 17 0.8115728335161378 1102 18 0.7840198928304076 1102 19 0.8430394831867716 1102 20 0.8342141813211512 1102 21 0.8236082336004813 1102 22 0.7542533625220074 1102 23 0.8405030218221796 1102 24 0.7853865552199082 1102 25 0.8156166402939959 1102 26 0.8633138604462047 1102 27 0.8300184382926119 1102 28 0.8220173949551111 1102 29 0.8505768297081602 1102 30 0.8889298563489092 1102 31 0.856973390068859 1102 32 0.9551992406296037 1102 33 0.915218916282349 1102 34 0.9232379164104287 1102 35 0.8522468494755984 1102 36 0.8754916759427616 1102 37 0.7452017782414515 1102 38 0.8136141784788791 1102 39 0.7849789592561791 1102 40 0.8615024588401354 1102 41 0.8682586980586677 1102 42 0.8963465713247487 1102 43 0.8991509564350366 1102 44 0.9393779770084691 1102 45 0.8110658169193741 1102 46 0.8993170295059087 1102 47 0.9418778384812398 1102 48 0.9156664185218375 1102 49 0.9617088123856377 1102 50 0.9197380805405189 1103 1 0.5371676489603594 1103 2 0.6431873302875744 1103 3 0.5700432593668872 1103 4 0.5195960209470556 1103 5 0.5945544773255236 1103 6 0.6862421005228967 1103 7 0.6846273839668982 1103 8 0.6525413465039165 1103 9 0.7549791110929505 1103 10 0.7605363591420513 1103 11 0.738488855620723 1103 12 0.7932093750280629 1103 13 0.7914889832132772 1103 14 0.8221732091671612 1103 15 0.8590545545198225 1103 16 0.951778477680989 1103 17 0.9623237134933476 1103 18 0.9445958285903799 1103 19 0.8169877493434257 1103 20 0.8223886100989546 1103 21 0.9414062898512014 1103 22 0.8884996001531604 1103 23 0.8811280938819692 1103 24 0.8566911934967152 1103 25 0.8699111325522324 1103 26 0.879524125829704 1103 27 0.8144850941079227 1103 28 0.8673716578427886 1103 29 0.8840997226798706 1103 30 1.0428128787230477 1103 31 0.9752910906663601 1103 32 0.9800116138891468 1103 33 0.9476928221603913 1103 34 0.9107975140963731 1103 35 1.0073029035153525 1103 36 0.9414472396368296 1103 37 0.9494914552101292 1103 38 0.9119661964921306 1103 39 0.864561656767826 1103 40 0.8989444888577012 1103 41 0.872804738330764 1103 42 0.7756209207519973 1103 43 0.7928980316552838 1103 44 0.753762060780943 1103 45 0.8288432216817654 1103 46 0.9267083972516019 1103 47 0.9232848525269617 1103 48 0.9605936127000874 1103 49 0.9674269687401207 1103 50 0.905764924084302 1104 1 0.4875608068143116 1104 2 0.6178941996954634 1104 3 0.4832658770530571 1104 4 0.5622069846160898 1104 5 0.6371644538428285 1104 6 0.6168854124959182 1104 7 0.6253261524786637 1104 8 0.5766772226742916 1104 9 0.6097908423382563 1104 10 0.6672296920673659 1104 11 0.7541623571614195 1104 12 0.7089436028524858 1104 13 0.7872436384974293 1104 14 0.8835341327331534 1104 15 0.8318347999027083 1104 16 0.7796411971227641 1104 17 0.908579422315178 1104 18 0.8541047485756437 1104 19 0.8000974845843913 1104 20 0.8557580567329524 1104 21 0.8721926401094109 1104 22 0.894849848495852 1104 23 0.8225330707390199 1104 24 0.8731278949423853 1104 25 0.7214327529025653 1104 26 0.8067704841858472 1104 27 0.7868223716059788 1104 28 0.8354595433029175 1104 29 0.8536266450221248 1104 30 0.8307577489315179 1104 31 0.802275376691604 1104 32 0.8949826089035327 1104 33 0.8985252954377856 1104 34 0.8696989326827662 1104 35 0.9096742229407013 1104 36 0.9378905977756418 1104 37 0.9237482492407523 1104 38 0.9027236923053721 1104 39 0.8987585339791551 1104 40 0.9059833413398835 1104 41 0.8974790235054044 1104 42 0.9088967360447242 1104 43 0.981039246342192 1104 44 0.8979274429164263 1104 45 0.9200979622722727 1104 46 0.9710575705860691 1104 47 0.9838984035206693 1104 48 1.0148557965791412 1104 49 1.0187792925693202 1104 50 1.0659395380627288 1105 1 0.42410764240344534 1105 2 0.5305047955807609 1105 3 0.43587060601649696 1105 4 0.5065903286493452 1105 5 0.5441769483025141 1105 6 0.5793669280853351 1105 7 0.5672019484604647 1105 8 0.539588498305001 1105 9 0.5993555760465341 1105 10 0.6547581550540613 1105 11 0.6881493874169422 1105 12 0.6469127662470626 1105 13 0.6940038232573755 1105 14 0.757595204824959 1105 15 0.8412462402400394 1105 16 0.802516261342312 1105 17 0.8073855998953618 1105 18 0.6702735684507246 1105 19 0.6967530986755861 1105 20 0.7928904624193791 1105 21 0.8283186442787738 1105 22 0.7727390069532376 1105 23 0.7768741520960774 1105 24 0.6663180978240675 1105 25 0.634104101885832 1105 26 0.7135221731299879 1105 27 0.714835400660867 1105 28 0.6902682952755868 1105 29 0.7081923846029312 1105 30 0.7762986077943737 1105 31 0.7310999966040299 1105 32 0.8451820602020703 1105 33 0.7669049109299806 1105 34 0.7325384747743229 1105 35 0.6926324179164709 1105 36 0.8365234927729688 1105 37 0.7753104774848509 1105 38 0.7808494512181667 1105 39 0.7436874536560225 1105 40 0.7796976879675626 1105 41 0.8030953188007288 1105 42 0.8419660578942754 1105 43 0.8056902566630058 1105 44 0.8034573373679379 1105 45 0.8146249737568567 1105 46 0.879641310075506 1105 47 0.8217773990587673 1105 48 0.8226733898107028 1105 49 0.7816719735843023 1105 50 0.8744973893587016 1106 1 0.35941963472069105 1106 2 0.4788254667778915 1106 3 0.4281701021405091 1106 4 0.44431718183153635 1106 5 0.4198756314934755 1106 6 0.37900697871825884 1106 7 0.46213992219679056 1106 8 0.4414212618539253 1106 9 0.4338646200165712 1106 10 0.4632621541702804 1106 11 0.4713790845210184 1106 12 0.4474515340095593 1106 13 0.5077213067556627 1106 14 0.5693292479316909 1106 15 0.5647309363761153 1106 16 0.5328682151332913 1106 17 0.6688370946243296 1106 18 0.5980974701449853 1106 19 0.5512828027017633 1106 20 0.5216991465806871 1106 21 0.5281937807871842 1106 22 0.5703179583842939 1106 23 0.5909853985762368 1106 24 0.6064598118712041 1106 25 0.5535719155903109 1106 26 0.5111032183800717 1106 27 0.5232818041282314 1106 28 0.5367962042425916 1106 29 0.5361327745126587 1106 30 0.6052757818855881 1106 31 0.5332655992512443 1106 32 0.6186688110933929 1106 33 0.6769039749616255 1106 34 0.6515830245243208 1106 35 0.62561930232782 1106 36 0.6696149900082702 1106 37 0.6742163945393074 1106 38 0.5917097780195633 1106 39 0.5988068333730752 1106 40 0.6347638784310305 1106 41 0.5761838065618079 1106 42 0.6956672346049473 1106 43 0.6458856443546921 1106 44 0.5363548750836316 1106 45 0.5338947809333092 1106 46 0.47268704955222773 1106 47 0.5737254085947043 1106 48 0.5895875177608616 1106 49 0.7209072514973656 1106 50 0.6989191007619979 1107 1 0.20534070351748568 1107 2 0.3803613522890714 1107 3 0.2741276259780143 1107 4 0.2717280734019454 1107 5 0.17596694226435972 1107 6 0.215156532931978 1107 7 0.13328890321707831 1107 8 0.14725931433673622 1107 9 0.1272927205124219 1107 10 0.2659972449554431 1107 11 0.3095080646625874 1107 12 0.2781145279458528 1107 13 0.28480115497637115 1107 14 0.17699038051468818 1107 15 0.2553564444739962 1107 16 0.2900725850655519 1107 17 0.23726696191724983 1107 18 0.12720418336985517 1107 19 0.16302282203060514 1107 20 0.1192834191218779 1107 21 0.2198067023300183 1107 22 0.08529345302385138 1107 23 0.20366426550941696 1107 24 0.1844835789020891 1107 25 0.23929285633149533 1107 26 0.2205408152147328 1107 27 0.3057438144652096 1107 28 0.22267314435057273 1107 29 0.22189403348641434 1107 30 0.2669207283691861 1107 31 0.3112425358118358 1107 32 0.21233099342212824 1107 33 0.3138221019991363 1107 34 0.27838050867887176 1107 35 0.21257611800545817 1107 36 0.18940438963667106 1107 37 0.1363026463071364 1107 38 0.17730990816620817 1107 39 0.10097425264163462 1107 40 0.03527180231278493 1107 41 0.010558585903801543 1107 42 0.027636125363294184 1107 43 0.14446038716805987 1107 44 0.09805552204869628 1107 45 0.06904745074994878 1107 46 0.21995725225973217 1107 47 0.2616952788868119 1107 48 0.2825654337632315 1107 49 0.2588195280373995 1107 50 0.36805348854435493 1108 1 0.10636328858349486 1108 2 0.28284818223220043 1108 3 0.14581561058524883 1108 4 0.11577093111718284 1108 5 0.11043369259405722 1108 6 0.1367005249984814 1108 7 0.080081581621819 1108 8 0.1839180879168154 1108 9 0.13567871208438476 1108 10 0.08115261566411647 1108 11 0.14962293281427463 1108 12 0.04689172737285929 1108 13 0.038304906588983556 1108 14 0.0427937526158928 1108 15 0.028070327674996065 1108 16 0.08468827877204177 1108 17 0.0388679977321047 1108 18 -0.04138038761676199 1108 19 0.03581153624016764 1108 20 0.02282577485286197 1108 21 0.0595869041779693 1108 22 -0.061294795917774536 1108 23 0.1412408680393611 1108 24 0.05549764060192075 1108 25 0.024727828592403966 1108 26 -0.002450422419499887 1108 27 0.031664393035129024 1108 28 -0.02367040816582744 1108 29 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-2.1182500895381366 2216 37 -2.079553016078556 2216 38 -2.0218608617787694 2216 39 -2.085952090370519 2216 40 -2.177936129946282 2216 41 -2.154915068593361 2216 42 -2.126800112908896 2216 43 -1.9563189601218411 2216 44 -1.8923640812553302 2216 45 -1.746834197719771 2216 46 -1.6726380283187154 2216 47 -1.7067065561901131 2216 48 -1.6924114617299537 2216 49 -1.6654489975982614 2216 50 -1.7045951064013671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80295.0 3 13303.0 4 23271.0 5 13913.0 6 10638.0 7 8422.0 8 7184.0 9 6407.0 10 6066.0 11 6002.0 12 6358.0 13 6481.0 14 6963.0 15 7777.0 16 8403.0 17 9179.0 18 10024.0 19 11185.0 20 13104.0 21 16202.0 22 20074.0 23 24430.0 24 28816.0 25 32492.0 26 35654.0 27 40573.0 28 47870.0 29 55387.0 30 64194.0 31 76549.0 32 93697.0 33 115290.0 34 145823.0 35 187525.0 36 243016.0 37 330072.0 38 525622.0 39 1376800.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75895058678978 17.903488810033455 14.045641807462742 26.291918795714025 2 14.871968939197359 19.950412324402777 38.06345349008731 27.114165246312556 3 17.646501471851778 24.44493314756633 29.484060502044656 28.42450487853724 4 12.325504146985132 16.504306339616708 36.44038688684862 34.729802626549535 5 13.935222796345299 37.380115642611386 34.24121014098403 14.443451420059278 6 32.80887991644788 36.53041162987748 17.092212922199764 13.568495531474877 7 28.46956538128594 31.562826051543574 21.637073246911303 18.330535320259187 8 26.90119207494704 34.14599014160803 19.71831858092572 19.2344992025192 9 26.75897172715491 14.989645702906124 18.636173075488763 39.6152094944502 10 14.50440238024718 28.613559869678774 33.60960176633727 23.272435983736774 11 35.268043713351396 22.430558846718913 23.44554465043537 18.85585278949432 12 23.628939900221912 24.322248704169304 30.24729638648432 21.80151500912447 13 28.46644394425998 21.330081984715683 25.727373018204553 24.476101052819786 14 22.52873061792405 20.4117518911165 26.958403919487218 30.101113571472233 15 23.851162550229706 28.4107527582412 23.639728582549587 24.09835610897951 16 23.95022725081715 27.471632021172375 25.03397048297598 23.544170245034493 17 22.708760160368477 27.28507306417109 26.417227331579706 23.588939443880726 18 22.742620374369974 26.71896830124535 27.402505350989355 23.135905973395325 19 23.71669299574659 26.430428480865054 27.3194860147073 22.533392508681054 20 23.77417298636206 25.91189562316492 27.419843479989048 22.894087910483975 21 24.021899245160057 25.705356589836185 27.285969348597096 22.986774816406662 22 23.74490263332864 25.513318254759675 27.250522898328423 23.491256213583263 23 23.657113619992057 25.524582769508346 27.122169935168543 23.69613367533106 24 23.2425276795495 25.724073748940373 27.184392816404262 23.849005755105864 25 23.48904353932887 25.58638391558829 27.30204695810651 23.62252558697633 26 23.30371184213361 25.935608263857475 27.390104267649022 23.370575626359898 27 23.424976067149316 25.944125713777915 27.19994398837759 23.43095423069518 28 23.512043009884035 25.813335703023917 27.243578609772552 23.431042677319503 29 23.605655952025117 25.891706452750828 27.045650997277583 23.45698659794647 30 23.51796611155302 25.95024818820189 27.124328906318578 23.407456793926514 31 23.596119188132565 25.560032301503675 27.107924881501468 23.73592362886229 32 23.4257742187805 25.89786222318557 27.082814450019587 23.59354910801434 33 23.6176154313798 25.72727679030574 27.01326100547144 23.64184677284302 34 23.749720338291723 25.72222720296023 27.04112662678307 23.486925831964978 35 23.605572433341123 25.755219579332113 27.078974036449555 23.56023395087721 36 23.641366023132136 25.771849701162587 26.95653765911453 23.630246616590746 37 23.667192904618116 25.56057264452878 27.09882253360959 23.673411917243516 38 23.7174541039261 25.729935359159157 27.00099882348824 23.551611713426503 39 23.825055908055308 25.907578809023146 26.761564763293627 23.505800519627922 40 23.714456768813804 25.683487438798398 27.14096585200922 23.461089940378574 41 23.59600408098439 25.711009236053723 26.93126663956778 23.761720043394106 42 23.814133409066134 25.774177179751884 26.831215013399053 23.58047439778293 43 23.66068688687158 25.613276467820416 26.879452936825228 23.846583708482775 44 23.76204335392063 25.904139855802775 26.560171546459244 23.773645243817352 45 23.6942821580159 25.89376274573563 26.50278548832722 23.909169607921253 46 23.902928270219373 25.790505907630255 26.35671997988708 23.949845842263283 47 23.76242580853555 25.905869369187496 26.396306913769667 23.935397908507287 48 23.66580315064909 26.186392408811308 26.360091562845554 23.787712877694045 49 23.87336826147439 25.843432777325564 26.36234823916517 23.920850722034874 50 24.012657706533616 25.880035498858835 26.303906085760914 23.803400708846638 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 750.0 1 536.0 2 322.0 3 552.0 4 782.0 5 870.0 6 958.0 7 950.0 8 942.0 9 1213.5 10 1485.0 11 2169.0 12 2853.0 13 5319.5 14 7786.0 15 10858.5 16 13931.0 17 15749.0 18 17567.0 19 18999.5 20 20432.0 21 22146.0 22 23860.0 23 28109.5 24 32359.0 25 38342.5 26 44326.0 27 51429.5 28 58533.0 29 67030.5 30 75528.0 31 84241.5 32 92955.0 33 103633.0 34 114311.0 35 126320.0 36 138329.0 37 148689.5 38 159050.0 39 170070.5 40 181091.0 41 193253.0 42 205415.0 43 213303.5 44 221192.0 45 231351.0 46 241510.0 47 250424.5 48 259339.0 49 262263.0 50 265187.0 51 267188.5 52 269190.0 53 264485.0 54 259780.0 55 250737.5 56 241695.0 57 226425.5 58 211156.0 59 192043.0 60 172930.0 61 151652.5 62 130375.0 63 111427.5 64 92480.0 65 77439.5 66 62399.0 67 51300.5 68 40202.0 69 31724.0 70 23246.0 71 18287.0 72 13328.0 73 10934.0 74 8540.0 75 6310.5 76 4081.0 77 3120.0 78 2159.0 79 1744.0 80 1329.0 81 1004.0 82 679.0 83 520.0 84 361.0 85 274.0 86 187.0 87 132.5 88 78.0 89 61.5 90 45.0 91 30.0 92 15.0 93 10.0 94 5.0 95 3.5 96 2.0 97 3.0 98 4.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014212418046433153 2 6.729364605318728E-4 3 3.4992695947657385E-4 4 0.001964974464753069 5 0.03709225770451683 6 0.026163769585479214 7 0.030039883598142803 8 0.023283601534402797 9 0.030793572433938498 10 0.031251169227100174 11 0.03803436874926146 12 0.03822279095821038 13 0.035531045116082884 14 0.04075303204981022 15 0.03693075295398918 16 0.04153363834402719 17 0.043417860433516434 18 0.04654028561038433 19 0.048532177533558665 20 0.044952155563529106 21 0.04551742219037588 22 0.04371395247615046 23 0.042691089056142016 24 0.040080095589278344 25 0.03911106708611245 26 0.04147980342718464 27 0.04161439071929102 28 0.04026851779822727 29 0.0390572321692699 30 0.03625781649345731 31 0.035046530864499936 32 0.031116581934993802 33 0.02322976661756025 34 0.020241928732798734 35 0.020914865193330606 36 0.02244916032334328 37 0.017361760681722318 38 0.018599963769100964 39 0.012920380042211958 40 0.0077791454837484505 41 0.0072946312321655014 42 0.006621694771633628 43 0.0026109934668636665 44 0.004010701304769962 45 0.002503323633178567 46 9.421110447446219E-4 47 2.9609204263402405E-4 48 4.5759679316167355E-4 49 1.3458729210637455E-4 50 3.2300950105529895E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3715061.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.83422182126549 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9927598581958 41.87162388911398 2 13.476653217682474 15.049168903287088 3 4.906270978902764 8.21813466453883 4 2.226548148901025 4.972703329658716 5 1.2658337058141742 3.5338419959631557 6 0.7328926882681708 2.4552295756769174 7 0.47661905825658984 1.8628157956058766 8 0.33505794369988307 1.4966159641213082 9 0.24920445578681844 1.252272317692407 >10 1.2205313371551474 12.141836801265741 >50 0.0801212466848096 3.070265925031785 >100 0.03653746657850136 3.4294692759309013 >500 5.334417430458335E-4 0.21252455125259515 >1k 4.36452330772435E-4 0.4334970108606757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4441 0.1195404328488819 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6917458421274912E-5 0.0 4 0.0 0.0 0.0 2.6917458421274912E-5 0.0 5 0.0 2.6917458421274912E-5 0.0 2.6917458421274912E-5 0.0 6 0.0 2.6917458421274912E-5 0.0 2.6917458421274912E-5 2.6917458421274912E-5 7 0.0 2.6917458421274912E-5 0.0 2.6917458421274912E-5 2.6917458421274912E-5 8 0.0 2.6917458421274912E-5 0.0 2.6917458421274912E-5 2.6917458421274912E-5 9 0.0 2.6917458421274912E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 10 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 11 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 12 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 13 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 14 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 15 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 16 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 17 0.0 5.3834916842549824E-5 0.0 5.3834916842549824E-5 5.3834916842549824E-5 18 0.0 5.3834916842549824E-5 0.0 8.075237526382474E-5 5.3834916842549824E-5 19 0.0 5.3834916842549824E-5 0.0 8.075237526382474E-5 5.3834916842549824E-5 20 0.0 1.0766983368509965E-4 0.0 8.075237526382474E-5 5.3834916842549824E-5 21 0.0 1.3458729210637457E-4 0.0 1.3458729210637457E-4 5.3834916842549824E-5 22 0.0 1.3458729210637457E-4 0.0 1.884222089489244E-4 5.3834916842549824E-5 23 0.0 1.3458729210637457E-4 0.0 2.9609204263402405E-4 5.3834916842549824E-5 24 0.0 1.3458729210637457E-4 0.0 3.4992695947657385E-4 5.3834916842549824E-5 25 0.0 1.6150475052764947E-4 0.0 3.4992695947657385E-4 5.3834916842549824E-5 26 0.0 1.6150475052764947E-4 0.0 3.768444178978488E-4 5.3834916842549824E-5 27 0.0 1.6150475052764947E-4 0.0 4.8451425158294845E-4 5.3834916842549824E-5 28 0.0 1.6150475052764947E-4 0.0 7.536888357956976E-4 5.3834916842549824E-5 29 0.0 1.6150475052764947E-4 0.0 0.0016150475052764948 5.3834916842549824E-5 30 0.0 1.6150475052764947E-4 0.0 0.0023418188826509175 5.3834916842549824E-5 31 0.0 1.6150475052764947E-4 0.0 0.004091453680033787 5.3834916842549824E-5 32 0.0 1.6150475052764947E-4 2.6917458421274912E-5 0.005975675769523031 5.3834916842549824E-5 33 0.0 1.6150475052764947E-4 2.6917458421274912E-5 0.008802008903756896 5.3834916842549824E-5 34 0.0 1.6150475052764947E-4 2.6917458421274912E-5 0.01222052612325881 5.3834916842549824E-5 35 0.0 1.6150475052764947E-4 2.6917458421274912E-5 0.01636581472013515 5.3834916842549824E-5 36 0.0 1.6150475052764947E-4 2.6917458421274912E-5 0.021157122319122083 8.075237526382474E-5 37 0.0 1.884222089489244E-4 2.6917458421274912E-5 0.027751899632334435 8.075237526382474E-5 38 0.0 1.884222089489244E-4 2.6917458421274912E-5 0.03402366744449149 8.075237526382474E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2755 0.0 26.754677 1 GTATCAA 6240 0.0 24.682547 1 GCGATAC 385 0.0 18.85999 14 TGCGATA 395 0.0 17.825474 13 TATCAAC 9005 0.0 16.760527 2 GCACCGT 490 0.0 16.61296 6 ATCAACG 9120 0.0 16.597878 3 TCAACGC 9145 0.0 16.480549 4 CAACGCA 9150 0.0 16.399847 5 GTGCGAT 485 0.0 15.878471 12 AACGCAG 9525 0.0 15.776008 6 CGCACGA 340 0.0 15.527782 35 ACCGTGC 695 0.0 15.511669 8 CGTGCCG 330 0.0 15.334407 10 GCGCGTT 190 1.0797521E-8 15.051051 36 ACCGTCG 165 5.422171E-7 14.667497 8 TATACTG 980 0.0 14.593646 5 TCATTCG 520 0.0 14.385623 24 GTACATG 9285 0.0 14.289331 1 GGTGCGA 515 0.0 14.098454 11 >>END_MODULE