FastQCFastQC Report
Mon 6 Feb 2017
SRR2077426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490431
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50400.023.1788431
GGTATCA24000.022.5502661
TCAACGC64850.017.6059824
TATCAAC65950.017.3459322
CAACGCA66050.017.2192555
ATCAACG66800.017.18963
AACGCAG67850.016.6959236
GTATAAT7300.016.2741641
GTACATG86150.014.6583251
ACGCAGA76450.014.6459747
TACATGG85350.014.6146562
TAATACC4750.014.357244
GCACCGT3400.014.2330126
CGCAGAG81150.013.7435018
TTATACT8100.013.5796274
ACATGGG89900.013.4088833
GTATAAA10050.013.3533911
ATTATAC7150.013.2292183
TATACTG9350.013.175685
AGAGTAC77100.013.15506511
Produced by FastQC (version 0.11.5)