FastQCFastQC Report
Mon 6 Feb 2017
SRR2077426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490431
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36900.10571760335614713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56300.023.5677221
GGTATCA26150.021.0366691
TATCAAC79000.016.9069022
ATCAACG80250.016.6163733
TCAACGC80400.016.4761334
CAACGCA82500.016.1100885
AACGCAG85450.015.6540066
GTACATG79650.013.3435091
ACGCAGA100150.013.3347287
GTATTAG5200.013.1179831
TACATGG79650.013.01180652
CGCAGAG103000.012.9230388
AGAGTAC97450.012.89160311
GAGTACT63200.012.60249712
ATTATAC6950.012.3477693
GTATACG1250.002663015212.3224411
AGTACTT65250.012.13911713
CAGAGTA107700.012.03222710
ATAACGC1651.2852479E-412.0023773
CTTAGAC7550.011.9493873
Produced by FastQC (version 0.11.5)