FastQCFastQC Report
Mon 6 Feb 2017
SRR2077425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163379
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.12945018601944314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.024.7621121
GTATCAA49850.022.8611411
TCAACGC64550.017.450194
CAACGCA64400.017.4214085
ATCAACG65400.017.2887543
TATCAAC64950.017.2405572
AACGCAG66900.016.7695886
CGGCACG2800.014.92964522
GTATTAG5100.014.66702651
ACGCAGA77150.014.2863517
GTACATG66600.014.2706181
CGCAGAG78850.013.9227588
TACATGG65900.013.9207732
AGAGTAC74950.013.62115811
TATACTG6300.013.6183035
GAGTACT45200.013.48369712
TCTATAC5150.013.2413663
ACATGGG68400.013.1214683
TTAGACG1200.00196273112.8334444
TATGCCG1557.1829614E-512.7734965
Produced by FastQC (version 0.11.5)