FastQCFastQC Report
Mon 6 Feb 2017
SRR2077425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163379
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.13640477476774046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62300.024.651161
GGTATCA27150.024.63621
TATCAAC87650.017.4705542
ATCAACG89250.017.182283
TCAACGC89000.017.0577664
CAACGCA90350.016.8272465
AACGCAG92900.016.4116846
GCGATAC1653.1128366E-816.00123614
ACGCGTA850.002980773315.5298756
GTATAAT7200.014.6682631
TAACGCA1201.1810406E-414.6680324
TGCGATA1809.057658E-814.66779913
CGCGTGA900.004360672614.66687210
GTACCGT2358.221832E-1014.0429736
TACGCGT950.006238929513.896035
ACGCAGA108450.013.8959997
GAGTACT73800.013.68398312
GTACATG71650.013.6037141
CGCAGAG111050.013.5904658
AGAGTAC106750.013.41679411
Produced by FastQC (version 0.11.5)