FastQCFastQC Report
Mon 6 Feb 2017
SRR2077424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454853
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42700.024.2663861
GGTATCA19900.024.0999161
TCAACGC57050.017.7756674
TATCAAC58300.017.6217022
ATCAACG57550.017.6207223
CAACGCA57350.017.489635
GCGATAC2400.017.41800914
AACGCAG60300.016.633526
GTACATG93200.015.2013451
TACATGG93400.014.7679492
TGCGATA2701.8189894E-1214.66843313
TCGATCG1057.1775017E-414.66291141
ACGCAGA70000.014.2988237
ACATGGG97250.014.1596583
GTCCTAA5350.013.5697631
CGCAGAG73300.013.5350398
CGACAGG4450.013.34876334
GAGTACT31350.012.91383712
CGCACGA3253.6379788E-1212.86141235
AGAGTAC72600.012.75911211
Produced by FastQC (version 0.11.5)