FastQCFastQC Report
Mon 6 Feb 2017
SRR2077424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454853
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48100.025.6181851
GGTATCA21100.022.942761
TATATCG1605.2750693E-1119.253395
CGCTATC3300.017.99691633
TATCAAC70300.017.4959182
CGCTACG4150.017.4950328
ATCAACG71550.017.2825163
TCAACGC71300.017.2816454
CAACGCA72000.017.1141245
CGAATCG2450.017.05829837
AACGCAG74350.016.6290116
TAGCGTA2403.6379788E-1215.5833687
AGCGTAT2550.015.5296738
ACCGTCG2152.1645974E-1015.3490958
ACCGTGC6150.015.0246428
ACGCGTA900.00436909514.66266743
GTACATG86950.014.576671
CGTCGTA2451.0004442E-1014.36779610
ATAGCGT2451.0004442E-1014.3669636
TATACAG8800.014.2525085
Produced by FastQC (version 0.11.5)