FastQCFastQC Report
Mon 6 Feb 2017
SRR2077423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255218
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.026.9559821
GTATCAA43050.022.026391
GCGATAC2950.017.15398614
CTAGCGG1053.7722944E-516.76460529
TGCGATA3100.016.32395613
TCAACGC57050.016.0421874
TATCAAC57850.016.0104892
CAACGCA57500.015.8008835
ATCAACG57800.015.7562063
TCTAGCG850.002978190815.53191428
AACGCAG59200.015.309046
CGATACT3550.014.25494215
GCGTTAA1554.5892175E-614.188416540
GTACATG79800.013.7298461
GTATGGA9850.013.6248851
GAACCGT2451.538865E-913.4678296
GTATAAG6600.013.3338411
TACATGG79400.013.2998472
TTATACT8450.013.2782044
ACGCAGA68700.013.0966157
Produced by FastQC (version 0.11.5)