FastQCFastQC Report
Mon 6 Feb 2017
SRR2077423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255218
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.026.9832081
GTATCAA48950.024.140051
CGTACAC2750.016.8023413
TATCAAC70300.016.6825242
ATCAACG70650.016.5373363
TCAACGC71050.016.4132654
TACCGTA1351.0862932E-616.2970647
CAACGCA71700.016.2654695
AACGCAG74550.015.7300256
TATAGCG900.00435529814.6696135
TACACCG2407.2759576E-1114.6696135
GCGATAC1951.5157639E-814.66871114
CGTGCCG3750.014.66735810
TTGCGCG1707.589806E-714.23727818
GTACATG76200.013.6013661
TACCGAT3250.013.53917
GTATTAC3750.013.496251
ACCGTGC6400.013.4068828
TACATGG75850.013.3441872
TTATACT8750.013.3275714
Produced by FastQC (version 0.11.5)